COLLEGE OF PHARMACY

3/F St. Theresa’s Building,

ADVANCED RESEARCH METHOD 1

INSTRUCTION: You can work in groups of 3. Submit your answers in 8.5”x13” .doc/x format at
[email protected] on or before 5:00PM today.
Perform the appropriate statistical analysis for the studies below using the steps in hypothesis
testing:
a. State the null and alternative hypothesis (2 points)
b. Level of significance (1 point)
c. Test statistics (1 point)
d. Critical Region (2 points)
e. Stata Output (4 points)
f. Statistical Decision (2 points)
g. Conclusion (3 points)
h. Linear regression model and interpretation (if applicable) (5 points)

1. The following are the weights (kg) and blood glucose levels (mg/100 ml) of 16 apparently healthy
adult males:
Weight (X) Glucose (Y)
64.0 108
75.3 109
73.0 104
82.1 102
76.2 105
95.7 121
59.4 79
93.4 107
82.1 101
78.9 85
76.7 99
82.1 100
83.9 108
73.0 104
64.4 102
77.6 87

A. What is the predicted glucose level for a man who weighs 95 kg? (3 points)

2. The objective of a study by Witteman et al. (A-26) was to investigate skin reactivity with purified
major allergens and to assess the relation with serum levels of immunoglobulin E (IgE) antibodies
and to determine which additional factors contribute to the skin test result. Subjects consisted of
patients with allergic rhinitis, allergic asthma, or both, who were seen in a European medical
center. As part of their study, the researchers collected, from 23 subjects, the following
measurements on specific IgE (IU/ml) and skin test (ng/ml) in the presence of Lol p 5, a purified
allergen from grass pollen. We wish to know the nature and strength of the relationship between
the two variables. (Note: The authors converted the measurements to natural logarithms before
investigating this relationship.)
IgE Skin Test
24.87 0.06
12.90 0.04
9.87 0.05
8.74 0.05
6.88 0.04
5.90 0.05
4.85 0.04
 3.53 0.06
2.25 4.33
2.14 0.55
1.94 0.05
1.29 0.45
0.94 0.40
0.91 0.48
0.55 4.46
0.30 4.10
0.14 7.43
0.11 4.46
0.10 6.63
0.10 49.13
0.10 36.47
0.10 52.86
0.10 47.50
Source: Data provided courtesy of Dr. Jaring S. van der Zee.

3. Garland et al. (A-27) conducted a series of experiments to delineate the complex maternal-fetal
pharmacokinetics and the effects of zidovudine (AZT) in the chronically instrumented maternal
and fetal baboon (Papio species) during both steady-state intravenous infusion and oral bolus
dosage regimens. Among the data collected were the following measurements on dosage
(mg/kg/h) and steady-state maternal plasma AZT concentration (ng/ml):
AZT
Dosage
Concentration
2.5 832
2.5 672
2.5 904
2.5 554
2.5 996
1.9 878
2.1 815
1.9 805
1.9 592
0.9 391
1.5 710
1.4 591
1.4 660
1.5 694
1.8 668
1.8 601
2.0 771
1.8 757
0.9 213
0.6 394
0.9 391
1.3 430
1.1 440
1.4 352
1.1 337
0.8 181
0.7 174
1.0 470
1.1 426
0.8 170
1.0 360
0.9 320
Source: Data provided courtesy of Dr. Marianne Garland.