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De La Salle Santiago Zobel: I. Materials

1. The document describes a lab experiment where students observe plant and animal cells under a microscope. They stain onion skin and Elodea leaf cells, as well as cells collected from their own wrists, with different dyes. 2. The procedures explain how to prepare slides of each cell type and switch between magnifications to view structures. Students are asked discussion questions about the cells' appearance and the functions of various organelles. 3. The goal is to compare and contrast plant and animal cells, noting both their similarities like membranes and nuclei, as well as differences such as chloroplasts only in plant cells.

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Noel Beth Borces Copon
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43 views

De La Salle Santiago Zobel: I. Materials

1. The document describes a lab experiment where students observe plant and animal cells under a microscope. They stain onion skin and Elodea leaf cells, as well as cells collected from their own wrists, with different dyes. 2. The procedures explain how to prepare slides of each cell type and switch between magnifications to view structures. Students are asked discussion questions about the cells' appearance and the functions of various organelles. 3. The goal is to compare and contrast plant and animal cells, noting both their similarities like membranes and nuclei, as well as differences such as chloroplasts only in plant cells.

Uploaded by

Noel Beth Borces Copon
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 2

Class No.

: DE LA SALLE SANTIAGO ZOBEL


___________ Br. Rafael Donato FSC Night High School
First Semester, AY 2019 – 2020

Science Grade 12

MODULE NO.: 1 TYPE: □F ■S ASSESSMENT NO.: 1

TITLE: Experiment 1: K X P U
Plant and Animal Cell Staining Lab
TOPIC: Unit 1: Cells X 30 20

NAME: GR. & SEC:


TEACHER: Mrs. Juliana V. Corpuz/Mrs. Rowena P. Guevara DATE:

Task 1: Plant and Animal Cell Staining Lab


Source: https://microscopy4kids.org/Comparing_Plant_and_Animal_Cells#GRADE_LEVELS
http://anatomycorner.com/cell/cheekcell.html
In this lab, students will obtain cells from their own wrists, stain them with methylene blue, and examine them
under the microscope. They will compare these with cells from the aquatic plant, Elodea. They will be able to
see common structures like the plasma membrane, cytoplasm and nucleus, as well as organelles specific to
plants, such as chloroplasts in the Elodea cells.

I. Materials:

 Light Microscope (40x, 100x, 400x magnification)


 Microscope slides, coverslips
 Medicine dropper
 Methylene Blue
 Elodea Leaf /Onion skin
 Tissue
 Clear tape
 Forceps/Tweezers
 Salt solution (6 g salt dissolved in 100 ml of water or approx. 2 teaspoons salt in a cup of water)
 Markers/Colored Pencils

II. Procedure A: Plant Cell Observation Using Onion Skin


1. Peel off a small section of onion skin
2. Place the onion skin in the center of the slide
3. Place the two drops of water on the onion skin. This is called a “wet mount”
4. Starting at one edge, gently lower a cover slip over the onion skin
5. Gently tap the slide with a pencil to remove any air bubbles
6. Place a drop of iodine at one edge of the cover slip. Touch the opposite edge of the cover slip with a
paper towel to draw the stain under the slip
7. Place the slide on the stage under low power. Use the coarse adjustment knob to focus
8. Rotate the nosepiece to medium power. Use the fine adjustment knob to focus. Observe what you see
9. Repeat step 8, but this time switch to high power and draw what you see (use a pencil)
10. After you draw your diagrams, rotate the nosepiece back to low power. Remove the slide and dispose
of the piece of onion, and wash the slide and cover slip.

Procedure B. Plant Cell Observation Using Elodea Leaf

1. Pick off an entire healthy-looking Elodea leaf, with fingers or small scissors and place it on the
microscope slide.
2. Add a drop of water (hypotonic solution) and a coverslip and observe the chloroplasts (green
structures) and the cell walls.
3. Add a drop of salt solution (hypertonic solution) to the side of the coverslip and observe the cell
shrinking (optional).
4. Illustrate your plant cell.

J. Corpuz/ R. Guevara Page 1 of 2


Procedure C: Animal Cell Observation Using Wrist Cells

1. Wash the underside of a wrist that will be sampled for epidermal cells with soap and water.
2. Stick a clean piece of clear tape on the underside of the washed wrist.
3. Gently remove the piece of tape from the wrist being careful to avoid getting fingerprints on the tape.
A pair of forceps/tweezers may help to remove the tape and avoid fingerprinting the tape.
4. Place the tape, sticky-side up, on a clean microscope slide.
5. Stain the top, sticky side of the tape with 2 or 3 drops of 1% methylene blue solution.
6. Use a dissecting needle to gently place a cover slip over the sticky tape. Lower the coverslip down onto
the tape and then remove the dissecting needle. This should help prevent staining your fingers.
Caution: Use methylene blue carefully. It will stain most items including skin, clothing, and table tops.
7. Examine the slide under a microscope. Look for cells with low power first, and then switch to high power
for details.
8. Sketch your skin cells and label appropriate parts.

Discussion: Use your laboratory activity to answer the following questions in complete sentences.

1. Why is methylene blue necessary?

2.The light microscope used in the lab is not powerful enough to view other organelles in the wrist cell.
What parts of the cell were visible?

3. List 2 organelles that were NOT visible but should have been in the wrist cell.

4. What color were the Elodea cells?

5. What were the green bodies inside the Elodea cells? Its function? Where were these green bodies mostly
located?

6. Did anyone notice a large space inside the cell? What is this large space? Its function?

7. Describe what happened to the Elodea cells in the presence of 6% salt solution.

  
Conclusion

J. Corpuz/ R. Guevara Page 2 of 2

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