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Human Cheek Cell Exp

The lesson plan is for a 9th grade biology class to prepare a stained temporary mount of human cheek cells. Students will scrape cheek cells using a toothpick, stain them with methylene blue, and view them under a microscope. They should observe irregularly shaped cells without cell walls but with distinct nuclei and lightly stained cytoplasm, identifying the cells as animal cells. Students will then answer questions about the lab procedure and their observations.

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Archna Verma
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5K views

Human Cheek Cell Exp

The lesson plan is for a 9th grade biology class to prepare a stained temporary mount of human cheek cells. Students will scrape cheek cells using a toothpick, stain them with methylene blue, and view them under a microscope. They should observe irregularly shaped cells without cell walls but with distinct nuclei and lightly stained cytoplasm, identifying the cells as animal cells. Students will then answer questions about the lab procedure and their observations.

Uploaded by

Archna Verma
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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LESSON PLAN

CLASS :IX (PRACTICAL) SUBJECT: BIOLOGY

NAME OF THE TEACHER: Ms Archna Verma

AIM /OBJECTIVE OF ACTIVITY: To Prepare Stained Temporary Mount of Human


Cheek Cells

THEORY:

Human Cheek Cell

As in all animal cells, the cells of the human cheek do not possess a cell wall. A cell
membrane that is semi-permeable surrounds the cytoplasm. Unlike plant cells, the
cytoplasm in an animal cell is denser, granular and occupies a larger space. The vacuole in
an an animal cell is smaller in size, or absent. The nucleus is present at the centre of the
cytoplasm.The absence of a cell wall and a prominent vacuole are indicators that help
identify animal cells, such as cells seen in the human cheek.

.Materials Required:

Slide, cover slip, methylene blue, glycerine, toothpick, dropper and microscope.

Lab Procedure

 Gently scrape the inner side of the cheek using a toothpick, which will collect some
cheek cells.
 Place the cells on a glass slide that has water on it.
 Mix the water and the cheek cells using a needle and spread them.
 Take a few drops of Methylene blue solution using a dropper and add this to the
mixture on the slide.
 After 2-3 minutes remove any excess water and stain from the slide using a blotting
paper.
 Take a few drops of glycerine using a dropper and add this to the test mixture.
 Take a clean cover slip and lower it carefully on the mixture with the aid of a needle.
 Using a brush and needle, press the cover slip gently to spread the epithelial cells.
 Remove any extra liquid around the cover slip using a blotting paper.
 Place this glass side on the stage of the compound microscope and view it.
Observations

 A large number of flat and irregular-shaped cells are observed.


 The cells do not have a cell wall. However, each cell has a thin cell membrane.
 A deeply stained nucleus is observed at the centre of each cell.
 No prominent vacuoles are observed in the cells.
 Large irregularly shaped cells with distinct cell walls.
 A distinct nucleus at the central part of each individual cell (dark blue in color).
 A lightly stained cytoplasm in each cell.

Conclusion

As the cells observed do not have a cell wall, nor a prominent vacuole, the cells of the
specimen on the slide are animal cells.
OBSERVATION SHEET:

The Human Cheek Cell

Sketch the cell at low and high power. Label the nucleus, cytoplasm, and cell membrane
of a single cell. Draw your cells to scale.

Low Power High Power

2. Why is methylene blue necessary?

3. The light microscope used in the lab is not powerful enough to view other organelles in the
cheek cell. What parts of the cell were visible.

4. List 2 organelles that were NOT visible but should have been in the cheek cell.

5. Is the cheek cell a eukaryote or prokaryote? How do you know?

6. Keeping in mind that the mouth is the first site of chemical digestion in a human. Your
saliva starts the process of breaking down the food you eat. Keeping this in mind, what
organelle do you think would be numerous inside the cells of your mouth?

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