Scribd
Upload
42 views

Ez-Cfu™ One Step: For Growth Promotion Testing

PRODUCTO DE MICROBIOLOGICS PARA ANALISIS CUANTITATIVO

Uploaded by

Luis Vignolo
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
42 views

Ez-Cfu™ One Step: For Growth Promotion Testing

PRODUCTO DE MICROBIOLOGICS PARA ANALISIS CUANTITATIVO

Uploaded by

Luis Vignolo
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 3

EZ-CFU™ ONE STEP

For Growth promotion Testing


• Quantitative
• Refrigerated storage
• Eight hour stability
• No dilutions required
• Peel-off Certificate of Assay
• Online Certificate of Analysis
• Traceable to reference culture
• Product warranty

EZ-CFU™
ONE STEP

EZ-CFU™ ONE STEP PRODUCT DETAILS


The Growth Promotion Test (GPT) is an important function in the United States (USP), European (Ph. Eur.) and
Japanese (JP) Pharmacopeias. EZ-CFU™ One Step quantitative microorganism preparations are used to perform
Growth Promotion Tests of culture media with ease. No dilutions are required. EZ-CFU™ One Step is designed to
deliver less than 100 CFU with each 0.1 mL inoculum. Each 2 mL vial of hydrated suspension offers 19 inocula.
EZ-CFU™ One Step can be used for up to eight hours after hydration if the suspension is refrigerated between uses.*
For added convenience, a peel-off Certificate of Assay is included for quality control documentation.

*Catalog numbers 0318Z and 0320Z are an exception to the eight hour stability and must be
used within 30 minutes of hydration.
EZ-CFU™ One Step kits include:
• 20 lyophilized pellets of a single quantitiative microorganism
• 10 vials of hydrating fluid (2 mL in each vial)
• Peel-Off Certificate of Assay

EZ-CFU™ One Step Instructions for Use

1 2 3
With a sterile forceps, transfer two (2)
pellets into the 2 mL vial of hydrating fluid.
(do not remove the desiccant from vial).
TWO PELLETS MUST BE USED TO OBTAIN
Remove the hydrating fluid vial and THE CHALLENGE CONCENTRATION OF
vial of lyophilized strain preparation While the pellets are <100 CFU PER 0.1mL. IMMEDIATELY
from refrigerated storage. Allow equilibrating, prewarm stopper and recap the pellet vial and return
unopened vial of lyophilized pellets the hydrating fluid to 35˚C the remaining lyophilized material to
to equilibrate to room temperature. (At least 30 minutes). refrigerated storage 2˚- 8˚C.

4 5
EZ-CFU™
ONE STEP

IMMEDIATELY
following
incubation,
vortex the
hydrated
material to
IMMEDIATELY recap the vial with the achieve a
hydrated material and place into a homogeneous
34˚C-38˚C incubator for thirty (30) suspension.
minutes to assure complete hydration.

6 With a sterile pipette,


transfer 0.1 mL of the
hydrated suspension to the
material being challenged
(0.1 mL contains <100 CFU).
Note: Remaining suspension
can be refrigerated and used
for up to 8 hours, with the exception
of cat. no 0320Z and 0318Z which
must be used within 30 min.

7 Proceed with the challenge procedure according to laboratory protocol. Discard any remaining
hydrated material in accordance with the laboratory protocol for disposal of biohazard materials.

Visit www.microbiologics.com or call 320.253.1640 to place your order!


EZ-CFU™ One Step
CAT# PRODUCT DESCRIPTION BSL COMMENT

0392Z Aspergillus brasiliensis ATCC® 16404™* 1


0998Z Bacillus cereus ATCC® 10876™* 1
0486Z Bacillus subtilis subsp. spizizenii ATCC® 6633™* 1
0582Z Bacillus subtilis subsp. spizizenii NCIMB 8054 1
0320Z Bacteroides fragilis ATCC® 25285™* 2
0805Z Brevundimonas diminuta ATCC® 19146™* 1
0488Z Burkholderia cepacia ATCC® 25416™* 2
0443Z Candida albicans ATCC® 10231™* 1
0332Z Candida albicans ATCC® 14053™* 1
0896Z Candida albicans ATCC® 2091™* 1
0379Z Candida albicans NCYC 1363 1
0318Z Clostridium perfringens ATCC® 13124™* 2
0487Z Clostridium sporogenes ATCC® 11437™* 1
0317Z Clostridium sporogenes ATCC® 19404™* 1
0580Z Clostridium sporogenes NCIMB 12343 1
0306Z Enterobacter aerogenes ATCC® 13048™* 1
0366Z Enterococcus faecalis ATCC® 29212™* 2
0497Z Enterococcus faecalis ATCC® 7080™* 2
0483Z Escherichia coli ATCC® 8739™* 1
0581Z Escherichia coli NCIMB 8545 2
0872Z Geobacillus stearothermophilus ATCC® 12980™* 1
0871Z Geobacillus stearothermophilus ATCC® 7953™* 1
0688Z Kocuria rhizophila ATCC® 9341™* 1
0813Z Lactobacillus fermentum ATCC® 9338™* 1
0687Z Listeria monocytogenes ATCC® 19115™* 2 serotype 4b
0242Z Micrococcus luteus ATCC® 4698™* 1
0353Z Pseudomonas aeruginosa ATCC® 27853™* 2
0484Z Pseudomonas aeruginosa ATCC® 9027™* 2
0902Z Salmonella enterica subsp. enterica serovar Choleraesuis 2
ATCC® 10708™*
0421Z Salmonella enterica subsp. enterica serovar Typhimurium 2
ATCC® 13311™*
0363Z Salmonella enterica subsp. enterica serovar Typhimurium 2
ATCC® 14028™*
0890Z Salmonella enterica subsp. enterica serovar Abony NCTC 6017 2 serovar Abony
0360Z Staphylococcus aureus subsp. aureus ATCC® 25923™* 2
0827Z Staphylococcus aureus subsp. aureus ATCC® 6538P™* 2
0485Z Staphylococcus aureus subsp. aureus ATCC® 6538™* 2
0579Z Staphylococcus aureus subsp. aureus NCIMB 9518 2
0371Z Staphylococcus epidermidis ATCC® 12228™* 1
0385Z Streptococcus pyogenes ATCC® 19615™* 2 group A

IMPORTANT: The mean assay value provided with the enumerated microorganism preparations is an average of the values obtained during the enumeration
phase of the quality control process. The quality control specification requires all values to fall within the target log range stated for a specific product. For
more information, please see mean assay value listed in the Product Overiew section at the beginning of this catalog.
* The ATCC Licensed Derivative Emblem, the ATCC Licensed Derivative word mark and the ATCC catalog marks are trademarks of ATCC.
Microbiologics, Inc. is licensed to use these trademarks and to sell products derived from ATCC® cultures.

LIT.014 REVISION 2011.AUG.01 Microbiologics® Retail Products: 39th Edition Page 65

You might also like