MATERIAL SAFETY DATA SHEET

Ethidium Bromide Solution

SDS/MSDS

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifiers
Product name : Ethidium Bromide Solution

1.2 Relevant identified uses of the substance or mixture and uses advised against
Identified uses : Laboratory chemicals, Industrial & for professional use only.
1.3 Details of the supplier of the safety data sheet
Company : Central Drug House (P) Ltd
7/28 Vardaan House
New Delhi-10002
INDIA
Telephone : +91 11 49404040
Email : [email protected]

1.4 Emergency telephone number

Emergency Phone # : +91 11 49404040 (9:00am - 6:00 pm) [Office hours]

SECTION 2: Hazards identification

2.1 Classification of the substance or mixture
Classification according to Regulation (EC) No 1272/2008
Acute toxicity, Inhalation (Category 3), H331
Germ cell mutagenicity (Category 2), H341
For the full text of the H-Statements mentioned in this Section, see Section 16.
2.2 Label elements
Labelling according Regulation (EC) No 1272/2008
Pictogram

Signal word Danger

Hazard statement(s)
H331 Toxic if inhaled.
H341 Suspected of causing genetic defects.
Precautionary statement(s)
P261 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P280 Wear protective gloves/ protective clothing/ eye protection/ face

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 protection.
P304 + P340 + P311 IF INHALED: Remove person to fresh air and keep comfortable for
breathing. Call a POISON CENTER or doctor/ physician.
P403 + P233 Store in a well-ventilated place. Keep container tightly closed.
Supplemental Hazard none
Statements

2.3 Other hazards

This substance/mixture contains no components considered to be either persistent, bioaccumulative and
toxic (PBT), or very persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher.

SECTION 3: Composition/information on ingredients

3.2 Mixtures
Synonyms : EtBr
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
Homidium bromide

Formula : C21H20BrN3
Molecular weight : 394,31 g/mol

Hazardous ingredients according to Regulation (EC) No 1272/2008

Component Classification Concentration
3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide
CAS-No. 1239-45-8 Acute Tox. 4; Acute Tox. 2; >= 1 - < 10 %
EC-No. 214-984-6 Muta. 2; H302, H330, H341
Index-No. 612-278-00-6

For the full text of the H-Statements mentioned in this Section, see Section 16.

SECTION 4: First aid measures

4.1 Description of first aid measures
General advice
Consult a physician. Show this safety data sheet to the doctor in attendance.
If inhaled
If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
In case of skin contact
Wash off with soap and plenty of water. Take victim immediately to hospital. Consult a physician.
In case of eye contact
Flush eyes with water as a precaution.
If swallowed
Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
4.2 Most important symptoms and effects, both acute and delayed
The most important known symptoms and effects are described in the labelling (see section 2.2) and/or in
section 11
4.3 Indication of any immediate medical attention and special treatment needed
No data available

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SECTION 5: Firefighting measures
5.1 Extinguishing media
Suitable extinguishing media
Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.
5.2 Special hazards arising from the substance or mixture
Carbon oxides, Nitrogen oxides (NOx), Hydrogen bromide gas
5.3 Advice for firefighters
Wear self-contained breathing apparatus for firefighting if necessary.
5.4 Further information
No data available

SECTION 6: Accidental release measures

6.1 Personal precautions, protective equipment and emergency procedures
Wear respiratory protection. Avoid breathing vapours, mist or gas. Ensure adequate ventilation.
Evacuate personnel to safe areas.
For personal protection see section 8.
6.2 Environmental precautions
Prevent further leakage or spillage if safe to do so. Do not let product enter drains.
6.3 Methods and materials for containment and cleaning up
Soak up with inert absorbent material and dispose of as hazardous waste. Keep in suitable, closed
containers for disposal.
6.4 Reference to other sections
For disposal see section 13.

SECTION 7: Handling and storage

7.1 Precautions for safe handling
Avoid contact with skin and eyes. Avoid inhalation of vapour or mist.
For precautions see section 2.2.
7.2 Conditions for safe storage, including any incompatibilities
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Containers which are
opened must be carefully resealed and kept upright to prevent leakage.
Recommended storage temperature 2 - 8 °C
Storage class (TRGS 510): Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous
materials causing chronic effects
7.3 Specific end use(s)
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated

SECTION 8: Exposure controls/personal protection

8.1 Control parameters
Components with workplace control parameters
8.2 Exposure controls
Appropriate engineering controls
Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling
the product.
Personal protective equipment
Eye/face protection
Face shield and safety glasses Use equipment for eye protection tested and approved under
appropriate government standards such as NIOSH (US) or EN 166(EU).

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 Skin protection
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique
(without touching glove's outer surface) to avoid skin contact with this product. Dispose of
contaminated gloves after use in accordance with applicable laws and good laboratory practices.
Wash and dry hands.
Body Protection
Complete suit protecting against chemicals, The type of protective equipment must be selected
according to the concentration and amount of the dangerous substance at the specific workplace.
Respiratory protection
Where risk assessment shows air-purifying respirators are appropriate use a full-face respirator with
multi-purpose combination (US) or type ABEK (EN 14387) respirator cartridges as a backup to
engineering controls. If the respirator is the sole means of protection, use a full-face supplied air
respirator. Use respirators and components tested and approved under appropriate government
standards such as NIOSH (US) or CEN (EU).
Control of environmental exposure
Prevent further leakage or spillage if safe to do so. Do not let product enter drains.

SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical properties
a) Appearance Form: liquid
b) Odour No data available
c) Odour Threshold No data available
d) pH 4,0 - 7,0
e) Melting point/freezing No data available
point
f) Initial boiling point and No data available
boiling range
g) Flash point Not applicable
h) Evaporation rate No data available
i) Flammability (solid, gas) No data available
j) Upper/lower No data available
flammability or

explosive limits
k) Vapour pressure No data available
l) Vapour density No data available
m) Relative density No data available
n) Water solubility No data available
o) Partition coefficient: n- No data available
octanol/water
p) Auto-ignition No data available
temperature
q) Decomposition No data available
temperature
r) Viscosity No data available
s) Explosive properties No data available
t) Oxidizing properties No data available
9.2 Other safety information
No data available

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SECTION 10: Stability and reactivity
10.1 Reactivity
No data available
10.2 Chemical stability
Stable under recommended storage conditions.
10.3 Possibility of hazardous reactions
No data available
10.4 Conditions to avoid
No data available
10.5 Incompatible materials
Strong oxidizing agents
10.6 Hazardous decomposition products
Other decomposition products - No data available
In the event of fire: see section 5

SECTION 11: Toxicological information

11.1 Information on toxicological effects
Acute toxicity
LD50 Oral - Rat - 1.503 - 2.177 mg/kg (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
LC50 Inhalation - Rat - 1 h - 0,0118 - 0,134 mg/l (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
LD50 Subcutaneous - Mouse - 110 mg/kg (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
LD50 Intravenous - Rat - 16 mg/kg (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
Skin corrosion/irritation
No data available (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
Serious eye damage/eye irritation
No data available (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
Respiratory or skin sensitisation
No data available (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
Germ cell mutagenicity
In vitro tests showed mutagenic effects (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
Carcinogenicity
IARC: No component of this product present at levels greater than or equal to 0.1% is identified as
probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
No data available (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
Specific target organ toxicity - single exposure
No data available (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
Specific target organ toxicity - repeated exposure
No data available
Aspiration hazard
No data available (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
Additional Information
RTECS: Not available
To the best of our knowledge, the chemical, physical, and toxicological properties have not been
thoroughly investigated. (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)

SECTION 12: Ecological information

12.1 Toxicity
No data available

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12.2 Persistence and degradability
No data available
12.3 Bioaccumulative potential
No data available
12.4 Mobility in soil
No data available (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
12.5 Results of PBT and vPvB assessment
This substance/mixture contains no components considered to be either persistent, bioaccumulative and
toxic (PBT), or very persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher.
12.6 Other adverse effects
No data available

SECTION 13: Disposal considerations

13.1 Waste treatment methods
Product
Offer surplus and non-recyclable solutions to a licensed disposal company.
Contaminated packaging
Dispose of as unused product.

SECTION 14: Transport information

14.1 UN number
ADR/RID: 2810 IMDG: 2810 IATA: 2810
14.2 UN proper shipping name
ADR/RID: TOXIC LIQUID, ORGANIC, N.O.S. (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
IMDG: TOXIC LIQUID, ORGANIC, N.O.S. (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)
IATA: Toxic liquid, organic, n.o.s. (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide)

14.3 Transport hazard class(es)

ADR/RID: 6.1 IMDG: 6.1 IATA: 6.1
14.4 Packaging group
ADR/RID: III IMDG: III IATA: III
14.5 Environmental hazards
ADR/RID: no IMDG Marine pollutant: no IATA: no
14.6 Special precautions for user
No data available

SECTION 15: Regulatory information

This safety datasheet complies with the requirements of Regulation (EC) No. 453/2010.
15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture
15.2 Chemical Safety Assessment
For this product a chemical safety assessment was not carried out

SECTION 16: Other information

Full text of H-Statements referred to under sections 2 and 3.
H302 Harmful if swallowed.
H330 Fatal if inhaled.
H331 Toxic if inhaled.
H341 Suspected of causing genetic defects.
Further information
The above information is believed to be correct but does not purport to be all inclusive and shall
be used only as a guide. The information in this document is based on the present state of our
knowledge and is applicable to the product with regard to appropriate safety precautions. It does
not represent any guarantee of the properties of the product. Central Drug House (P) Ltd and its
Affiliates shall not be held liable for any damage resulting from handling or from contact with the
above product. See www.cdhfinechemical.com for additional terms and conditions of sale.
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