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Data Pengamatan Farkin Darah

This document presents data and calculations from a pharmacokinetic study of paracetamol. It includes: 1) Calibration curve data from paracetamol standards used to determine the linear regression equation. 2) Absorbance readings from blood samples taken from subjects over time used to calculate pharmacokinetic parameters. 3) Determination of elimination constant, half-life, volume of distribution, clearance, and AUC from concentration-time data. 4) Results of an accuracy study showing recovery percentages between 74-91% and systematic error below 26%.

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Meidi Rani
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28 views8 pages

Data Pengamatan Farkin Darah

This document presents data and calculations from a pharmacokinetic study of paracetamol. It includes: 1) Calibration curve data from paracetamol standards used to determine the linear regression equation. 2) Absorbance readings from blood samples taken from subjects over time used to calculate pharmacokinetic parameters. 3) Determination of elimination constant, half-life, volume of distribution, clearance, and AUC from concentration-time data. 4) Results of an accuracy study showing recovery percentages between 74-91% and systematic error below 26%.

Uploaded by

Meidi Rani
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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DATA PENGAMATAN DAN PERHITUNGAN

A. Data Kurva Kalibrasi


Tabel 1: Data Kurva Kalibrasi pada λmax 435 nm
Konsentrasi (ppm) Serapan (A)
200 0,599
300 0,661
400 0,711
500 0,804
700 0,829

Gambar 1: Kurva Kalibrasi Larutan Standar Parasetamol

Berdasarkan data diatas, didapatkan persamaan regresi linear y = a + bx


Nilai a = 0,519
Nilai b = 0,0005
Harga r = 0,9593
Persamaan garis:
y = 0,519 + 0,0005x
B. Data Serapan Sampel

Tabel 2 : Data Serapan Sampel Darah pada λmax 435 nm

Jam ke - Serapan (A) Cp (ppm)


Data 1 Data 2 Data 1 Data 2
0,25 0,935 0,965 832 892
0,50 0,925 0,957 812 876
1,0 0,912 0,867 786 696
2,0 0,815 0,849 592 660
4,0 0,741 0,806 444 574
6,0 0,736 0,778 434 518
9,0 0,552 0,598 66 158
12,0 0,552 0,552 66 66

Perhitungan Analisis

Kurva hubungan antara konsentrasi obat dalam plasma terhadap waktu:

DATA 1
Gambar Semilog
Berdasarkan hasil plotting pada semilog:
a. Konstanta Eliminasi (K)
k = 0,21567 jam-1

b. Waktu Paruh (t1/2)

t = 3,2132 jam

c. Volume Distribusi
Diketahui dosis tunggal parasetamol = 500 mg

Vd = 574,712 ml

d. Klirens (Cl)
Cl = k x Vd
= 0,21567 jam-1 x 574,712 ml
Cl = 123,948 ml/jam

e. AUC (Area Under Curve)

[AUC] = 4033,94
[AUC] = 4,03394 mg jam/ml
DATA 2
Gambar Semilog
Berdasarkan hasil plotting pada semilog:
a. Konstanta Eliminasi (K)
k = 0,2015 jam-1

b. Waktu Paruh (t1/2)

t = 3,4392 jam

c. Volume Distribusi
Diketahui dosis tunggal parasetamol = 500 mg

Vd = 520,83 ml

d. Klirens (Cl)
Cl = k x Vd
= 0,2015 jam-1 x 520,83 ml
Cl = 104,947425 ml/jam

e. AUC (Area Under Curve)

[AUC] = 4764,267
[AUC] = 4,764267 mg jam/ml

C. Perhitungan UPK (Uji Perolehan Kembali)


Rumus yang digunakan:

Kesalahan sistematik = 100% - R%

Tabel 3 : Hasil Perhitungan UPK dan Kesalahan Sistematik

Konsentrasi Serapan (A) Kadar UPK (%) Kesalahan


(ppm) Terukur Sistematik
(ppm) (%)
250 0,612 186 74,4 25,6
500 0,714 390 78 22
750 0,860 682 90,93 9,07

a) Konsentrasi 250 ppm


Konsentrasi terukur:
A(250) = 0,519 + 0,0005 x
0,612 = 0,519 + 0,0005 x
x = 186 ppm
Nilai UPK

UPK = 74,4%
Kesalahan Sistematik = 100% - 74,4% = 25,6%

b) Konsentrasi 500 ppm


A(500) = 0,519 + 0,0005 x
0,714 = 0,519 + 0,0005 x
x = 390 ppm

Nilai UPK

UPK = 78%
Kesalahan Sistematik = 100% - 78% = 22%

c) Konsentrasi 750 ppm


A(750) = 0,519 + 0,0005 x
0,860 = 0,519 + 0,0005 x
x = 682 ppm
Nilai UPK

UPK = 90,93%
Kesalahan Sistematik = 100% - 90,93% = 9,07%

D. Data Hasil Perhitungan


a. Perhitungan Analisis Parameter Farmakokinetika
Parameter Data 1 Data 2
Konstanta eliminasi (k) 0,21567 jam-1 0,2015 jam-1
Waktu paruh (t1/2) 3,2132 jam 3,4392 jam
Volume distribusi 574,712 ml 520,83 ml
Klirens 123,948 ml/jam 104,947425 ml/jam
[AUC] 4,03394 mg jam/ml 4,764267 mg jam/ml

b. Perhitungan UPK
Parameter 250 ppm 500 ppm 750 ppm
Konsentrasi Terukur 186 ppm 390 ppm 682 ppm
UPK (%) 74,4 78 90,93
Kesalahan Sistematik (%) 25,6 22 9,07

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