Labgard Class II, Type A2 Laminar Flow Biological Safety Cabinet Models NU-440-300/400/600 Bench/Console Operation & Maintenance Manual June, 2005 (Series 30 and Higher) Revision 3 ®Q® @® NU-440-400/600 Only Sliding Window Only Manufactured by: NuAire, Ine. 2100 Fernbrook Lane Plymouth, Minnesota USA 55447 Toll Free 1-800-328-3352 In MN 763-553-1270 Fax 763-553-0459 oMo137Congratulations! You have just purchased one of the finest Laminar Flow Biological Safety Cabinets available. With proper care, maintenance (certification), and laboratory procedure, this cabinet will give you years of product and personnel protection from particulate contaminants as prescribed in NSF/ANSI 49:2002. Please read this manual carefully to familiarize yourself with proper installation, maintenance and operation of the cabinet. Acknowledgment ‘Nusite, ln,ainovleges that some matral in thie manual elect information supplied by the Nato! Isis of Hel personnel both in verbal and writen specifications. In patil, NoAineseknowledges that information a Section & was obtained om the ollowing Sources: 1. Testnicl Rspon No, FPS 56500000001, Prepared by Dow Chemical Co forthe Nations) Cancer Instins, May 1, 1972. 2, Stolar MH, Power LA, Vielo CS: Recommendations for handling cytotoxic drs in hospitals. Am J Hosp Ph 1983;40: 1163-1171, 3. Anderon RW,, Director of Phare, University of Texas, MD. Anderson Hospital and Tuma Instat t Houten M0137ABOUT THIS OPERATION & MAINTENANCE MANUAL ‘The information contained in this manual is intended to reflect our current production standard configuration model along with the more frequently purchased options. Any unique additions/modifications/shop drawings are appended in the back fap of this manual, along with any modifications and/or additions to procedures as outlined in this manual. A copy of the original factory test report is also appended to this manual. In case this manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A replacement copy can be obtained by calling or writing NuAire, Inc. stating the ‘model number and serial number and a brief description of the information desired. M0137Labgard Class I, Type A2 Laminar Flow Biological Safety Cabinet Models NU-440-300/400/600 ‘operation & Maintenance Manual ‘TABLE OF CONTENTS Section No. 1 General Description Section NO. 2 nuns : ee Models & Options Section No. 3. seem Warranty Section NO. 4senensr = = Shipments Section No. 5. ' sewundnstallation Instractions 5 : : Location pases . Set-up Instructions 53 . . ss Certification Testing Methods and Equipment Section No. 6. Operating th NU-0 Electronic Airflow Control System rm Operating Guidelines ‘Operating Sequence z Ergonomics ssnnsesCleaning Procedure Antineoplastic Decontamin: General Maintenance Decontamination Fluorescent Lamp Replacement HEPA Filter/Motor Replacement Procedure Section No, 7 Thon Th oso Sliding Window Replacement & Adjustment TS cr cone = Airflow Control System Setup & Calibration 76. : Filter Integrity Check TT crn . : Cabinet Leak Tightness Test 78. : Cleanliness Classification Test for Pharmacy Application 79) Main Control Board Description & Replacement 710. Airflow Probe Description & Replacement Section NO. 8 Error Indicators, Troubleshooting, Diagnostics & Airflow Probe Performance Verification, Option Mode Section No. 9 Remote Contacts Section No. 10. (Optional Equipment 101 .-Ulraviolet Light Section No. 11 Insert [Blectrical/Environmental Requirements Replacement Parts List MANUAL DRAWINGS ACDOS1BL neni --NU-#10 Airflow Sehematic BCD.05004 “NU-440-300 Specification Drawing BCD-05005... - svueesNU-640-400 Specification Drawing BCD.05006, “us usNU-640-600 Specification Drawing BCD-05154 vanes Dain Valve Installation BCD-05166, "Front Panel ‘ASSEMBLY DRAWINGS BCD-05147, rn Base Stand Assembly BCD-05146, nrnnsneneBase Stand Storage Cabinet Assembly BCD.OSIAS, cesar COMKOI Center & Front Decorative Panel Assembly BCD-05144.. ener Stiding Window Assembly & Adjustment BCD.05153, vse Base Cabinet Assembly ELECTRICAL SCHEMATICS BCD-05123 vronesean .NU-440-300/400/600 Electical Schematic BCD.08S5, VO Board BCD-084S4 on snes onttO] Board Display omo137 4Labgard Class I, Type A2 Laminar Flow Biological Safety Cabinet Models NU-440-300/400/600 MANUFACTURED BY: ‘Nudire, Inc. - Plymouth, Minnesota, U.S.A. 1.0 General Description ‘The LABGARD Model NU-440 Laminar Flow Biological Safety Cabinet (LFBSC) is a bench/table top model, optionally available with a base support stand, for operation as a console model. ‘The Laminar Flow Biological Safety Cabinet, (LEBSC) is a product resulting from the development of the “laminar flow” principle and the application of environmental controls as required in the field of biological research or chemical containment. The LFBSC, when used with proper technique, is an effective laboratory aid in obtaining the optimum control over product quality while reducing the potential for exposure of both product and personnel to airborne biotogical or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product operations, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia. ‘The NU-440 bench LFBSC is known as Class Il, Type A2 Biological Safety Cabinet. This is possible since ‘NuAire's cabinet conforms to the following requirements: 1. Maintains inflow velocity of 100 LFPM (,51 M/S) through the work access opening. 2, Has HEPA filtered dowaflow air that is mixed with the inflow air from a common exhaust plenum, 3. Exhaust airflow can either be room re-circulated or exhausted outside using a canopy exhaust transition. 4, Hasall biologically contaminated ducts and plenums under negative pressure or surrounded by negative pressure, (Type Al permits positive pressure contaminated ducts and plenums). M0137 5DATE __ RF TCHR) &/20/01 | JR [DSH BF DESCRIPTION we ACD-05181 |B) DRAWING NUMBER B [8061 [ADDED ARMREST REV] Eco. oMo1372.0 Models & Features ‘The model NU-440, Class I, Type A2 Laminar Flow Biological Safety Cabinet is manufactured in three sizes: 3 ft, 4 ft, and 6 ft M0137 7aurea oe | [Ae [eae — OL TO isla at tor M0137£0 om mexoenes uo 1 = 1 wn we FE we 2. Lag 2 eel Siva tr Git omo137Hi 900S0-d9@ Ta La DOl_rere7e wit] aauaKrTp ue att cal“ S3uaia a a GAS 5 Vas BRST Paes we « cae Bitte casnased co SS ———————— oltre, FRI ro aT TER 0M0137,3.0 Warranty NuAire, Inc. warrants that it will repair F.O.B. its factory or furnish without charge F.O.B. its factory a similar part to replace any material in its equipment within 36 months after the date of sale if proved to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories are warranted only to the extent guaranteed by the original manufacturer, and this warranty shall not apply to any portion of the equipment modified by the user. Claims under this warranty should be directed to NuAie, Inc. setting forth in detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment. This warranty shall not apply to any NuAjre product or part thereof which has been subject to misuse, abuse, accident, shipping damage, improper installation or service, or damage by fire, flood or acts of God. Ifthe serial number of this product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety. ‘The warranty is for the sole benefit of the original purchaser and is not assignable or transferable, Prior to returning any item, for any reason, contact NuAire for a Return Authorization Number. This number must accompany all returns. Any product shipped to NuAire without this number will be returned refused shipment or collect freight 4.0 Shipments NuAire takes every reasonable precaution to assure that your LABGARD cabinet arrives without damage. Motor carriers are carefully selected and shipping cartons have been specially designed to insure your purchase, However, damage can occur in any shipment and the following outlines the steps you should take on receipt of a NuAire LABGARD cabinet to be sure that if damage has occurred, the proper claims and actions are taken immediately. 4.1 Damaged Shipments 4.1.1 Terms are factory, unless stated otherwise. ‘Therefore, it is important to check each shipment before acceptance, 4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis of your filing the claim against the carrer. 4.1.3. If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and request an inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date and that he failed to comply with the request. This along with other papers in the customer's possession will support the claim. oMo137 n5.0 5a Installation Instructions Location Within the laboratory, pharmacy, etc., the ideal location of the biological safety cabinet is away from personnel traffic lanes, air vents (in or out), doors andlor any other source of disruptive air currents, SEWORK ‘THE EXHAUST FILTER AREA IS ESPECIALLY SUSCEPTIBLE TO DISRUPTIVE AIR CURRENTS FROM AIR VENTS. The Electronic Airflow Control System's exhaust probe is located just above the exhaust HEPA filter and if disruptive air currents are present, the exhaust probe could be influenced by them and indicate disruptive readings on the front panel display. Ifdrafts or other disruptive air currents exceed the inflow velocity of the cabinet through the access opening, the potential exists for contaminated air to exit or enter the work zone area of the cabinet. It depends on the severity of the air current. REMEMBER: A BIOLOGICAL SAFETY CABINET IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE. Where space permits, « clear 12" (30Smm) area should be permitted on each side of the cabinet for maintenance purposes. The electrical outlet into which the cabinet is connected should be readily accessible for maintenance purposes. A MINIMUM CLEARANCE OF 6" (152MM) IS REQUIRED FROM THE TOP OF THE CABINET ‘TO THE CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX. HOWEVER, FOR CERTIFICATION OR COMMISSIONING, 18 INCHES (451MM) IS REQUIRED TO OBTAIN VALID EXHAUST MEASUREMENTS. Jf this cabinet is used in a pharmacy application, Per OSHA, NIOSH, and ASHP, itis strongly recommended that the eabinet be exhausted to the outside. In addition, if this cabinet is used in microbiological application with minute quantities of volatile toxie chemicals and tracer amounts of radionuclides, Per CDC/NIH and NSF it is strongly recommended that the eabinet be exhausted to the outside. NuAire offers two general categories of exhaust transitions, which will capture the exhaust efflux from the cabinet. These are: Canopy, Thimble or Air Gap Exhaust Transitions (with and without integral fan) Gas-Tight Exhaust Transitions CAUTION: THE EXHAUST SYSTEM SHOULD BE FITTED WITH A BACKDRAFT DAMPER TO PREVENT REVERSING OF AIRFLOW IN THE SYSTEM. ‘Both types of transitions have some common attributes, in addition to some that are unique. NuwAire strongly recommends a canopy or thimble exhaust for most applications. See separate instruction sheets for ¢ discussion of exhaust transitions and installation requirements. om0137 252 omo137 Set-Up Instructions Remove outer shipping protection (carton or crating). ‘The cabinet is fastened to the base skid and itis usually the best procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate ‘ease in handling. It can then be removed from the skid by removing the banding holding the cabinet to the skid. It may be necessary to remove the Control Center in order to gain passage through a doorway. It can easily be removed by following the instructions on drawing BCD-05145. 521 52.2 523 Base Stand Assembly ‘The base stand is shipped knocked down in a separate carton and is assembled per drawing BCD-05147 if accompanied with the unit. Remove the banding holding the cabinet to the base skid. Lift the cabinet from the base skid and place on the floor. Now lift the cabinet on top of the base and bolt the base stand to the cabinet using two 3/8" - 16 x 3/4 bolts and washers provided for the front base stand tabs and two 1/4" acorn nuts for the rear weld studs. Place the cabinet in its desired location. ‘The base stand storage cabinets will usually be shipped according to customer requirements. If't is shipped unassembled, it can be assembled per drawing BCD-05146. It is recommended that the upper and lower base stand braces be installed first, then the rear and bottom panels (the end panels are always prefastened). Once assembled, fasten the cabinet per the above instructions. Remove the plastic cap protecting the drain valve threads and install the drain valve on the bottom right front of the cabinet using the furnished Loctite 242 to the threads, and rotate the valve body until itis secure (see ACD-01855). Leveling Using a level placed on the work tray, adjust the leg levelers, first, end to end, then, front to back. The NSF approved leg levelers provide a + 3/4" (20mm) adjustment, Bench Installation (BCD-05154) Place the cabinet on the bench with approximately a 2" (SOmm) overhang clearance for installation of the rain valve. If the drain valve is not desired, place the cabinet in its desired location and using RTV caulk, seal all around the base of the cabinet and the bench. This provides a tight seal to prevent bench spills from migrating under the cabinet. Ifa drain valve is desired, remove the handle from the valve stem to gain clearance for valve body rotation. Add Loctite 242 (furnished) to the threads and rotate valve body until secure, with the valve stem (for handle) on the left side. Re-install handle to valve stem. Adjust the cabinet on bench to provide a 1-1/2” (38mm) overhang and seal the interface of the bench and cabinet, using RTV caulk as above. B524 Gas Serviee NuAire doesn't recommend the use of natural gas within the BSC, but if gas service is determined to be necessary for the application, appropriate safety measures must take place. All NuAire BSC’s have precautionary warning labels that say the following: CAUTION Use of toxic, explosive or flammable substances in this cabinet should be evaluated by your appropriate safety personnel (Once the determination has been made by the appropriate safety personnel, the application of natural gas, must be performed in accordance to national, state and local codes. IT IS ALSO STRONGLY RECOMMENDED THAT AN EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST OUTSIDE THE BSC ON THE GAS SUPPLY LINE. All NuAjre BSC’s meet the safety requirements of UL and CSA for Laboratory Equipment. To comply ‘with these safety requirements, NuA\ire uses only certified gas valves. In addition, if external piping is required, only black pipe is used for this application. As previously stated, NuAire doesn't recommend the use of natural gas within the BSC and ASSUMES NO RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The Bunsen burner flame within the BSC not only contributes to heat build-up; is also disrupts the laminar air stream, which must be maintained for maximum efficiency. IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS STRONGLY RECOMMENDED. DO ‘NOT USE CONSTANT FLAME GAS BURNERS. During se, the Bunsen burner should be placed to the rear of the workspace where resulting air turbulence will have a minimal effect. 5.2.5 Plumbing Services Ground key cocks with the type of service specified by the removable button on the handle, are located in the work zone. The ground key cocks are not recommended for pressure over 30 p.s.i (2.0 BAR). If necessary, reducing valves should be installed external to the cabinet if necessary. Ground key cocks should never be used for flammable gases or oxygen service. A special needle valve for oxygen service or certified valve is required and available upon request. Extemal connection is to 3/8 inch NPT coupling in the inner side walls. Connection to plant utilities should be made with proper materials for the individual service and according to national and/or local codes. Observe all labels pertaining to the type of service and operating pressure. M0137 “G|_¢SISO-G9d saan Ta Yi RO] Ware NLT WSN AWA Nived OPV LEY /SEV/OGH/ EBV /GR-AN @ e - 3anod “ANVW3S NOOTUS ALA SNISn “aD HONE HLA SOW-DI3INI_W3S QNY BNVIRGGAD «2/71 V HUA HONG SHL NO I3MIBVD HL 1SnraV ¢9 YOUS AWA DL SUNWH TIVISNI-3Y CS TWADKS SY 30IS 1577 NO_IGLS AWA HLIA ‘Sung3s WANT ADE SA-WA “SIVIDY ONY savauHl OL Caisivend 2v2 L191 ay cr ave SATWA NIVEL 40 NOLWT WISH 204 ‘NoTIWLDe A0GE AWA 804 SDMA 9 0 KGLS 3AWA WOR SHIM SADKON Ce anwn swan 271-1 a are ee eo forse NLS 2 = nor “2 SUNOS NE NADHS, SY SATWA Nava AL “THISNE CNY “V28 RGA aos! SINGINDD Un ¥ ~ NULWD 3ADAE C2 "IATA iva 20 MOLWTWLsME wos soMRHES TD SMHRGGAD SaMOH 2 ATRLVHDNDEGY LIA WONGE V NO) LSMIGW) SMI 3OVId Ct saree are ) 1 anoL “S1S34 11 OWN woe Hanae 3a OL aaT¥3S AVBL ODA 3 ATE Wd NIV ¥ SiH TNievo ALAS Wade seIvAN SH omo1375.2.6 Electrical Services ‘The NU-440 series Biological Safety Cabinets may be “hardwired” (optional) or connected via an electrical power cord which is standard. The unit requires 115 VAC, 60 Hz, single phase (current rating, varies per cabinet size, reference Electrical/Environmental Requirements). It is recommended that power to the unit be on its own branch circuit, protected with a circuit breaker respectively or fuse at the distribution panel PLEASE NOTE, THIS UNIT CONTAINS ELECTRONIC BALLASTS FOR THE FLUORESCENT LIGHTING, ELECTRONIC BALLASTS OPERATE WITH HIGH INRUSH CURRENT, IT IS NOT RECOMMENDED TO USE THIS PRODUCT WITH GROUND FAULT CIRCUIT INTERRUPTERS (GFCI'S) BECAUSE THE BALLASTS MAY CAUSE THE GFCI TOTRE. 5.2.7 inal Assembly REMOVE THE PROTECTIVE CARDBOARD COVER OVER THE EXHAUST HEPA FILTER, located under the protective screen if in place. Attach the exhaust sensor shroud over the exhaust sensor. ‘The shroud should be placed as close as possible to the exhaust HEPA filter without coming in contact. ‘The probe gasket should be tightly against the sensor shroud to prevent sneak airflow paths. The exterior surface and viewing glass are easily cleaned with any mild household detergent cleaner using a soft cloth Harsh chemicals, solvent-type cleaners and abrasive cleaners should not be used. Do not attempt to clean the HEPA filter media. Cabinet interior walls or work surface are easily cleaned with any mild household detergent cleaner using a soft cloth. Turn the cabinet on and let it operate for (60 minutes before using it as a LEBSC. PROBE GASKET EXHAUST SENSOR PROBE EXHAUST SENSOR SHROUD 14-20 KNURLED NUT (2) EXHAUST HEPA FILTER EXHAUST SENSOR SHROUD INSTALLATION oMo137 653 Certification Testing Methods and Equipment ‘After installation and prior to use, NuAjte recommends that the cabinet be certified or commissioned to factory standards. Ata minimum, the following tests should be performed HEPA filter leak test Downflow velocity test Inflow velocity test Airflow smoke patterns ‘The testing methods and equipment required are specified on the factory inspection report included with this rmanval (see insert in back cover). IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN ‘WHO IS FAMILIAR WITH THE METHODS AND PROCEDURES FOR CERTIFYING BIOLOGICAL SAFETY CABINETS (SEE INSERT). AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT A MINIMUM ON AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE OR MAINTENANCE ACTION OR ANY TIME THE OPERATOR FEELS IT IS NECESSARY. Note that the LABGARD cabinets, filters and seals provide premium performance; Quality Control in both design and manufacturing assure superior reliability. However, protection to both product and operator is so vital that certification to the performance requirements should be accomplished as stated to ensure biological safety established by the factory standards. oMo137 ”Labgard Class IT, Type A2 ‘Laminar Flow Biological Safety Cabinet Models NU-440-300, 400, 600 Catalog Number Catalog Namber NU-440.300 ‘NU-440-400 NU-480-600 Nominal 3 foot (0.9m) | Nominal 4 foot (1.2m) [Nominal 6 foot (1.8m) eriormance Specieions 1 Personal Protection [NSF Std. No. 49 NSF Std. No. 43 [NSF Std. No. 49 2. Product Protection NSE Sul No. 99 NSE Std No. 48 NSF Std. No, 38 TSE Sid No. 49 Cia Classi Type AT (Cassi, Type AP (Cass. Type 2 ‘Spicof Cabinet ‘Bench tp/console wise sand/storage eabinet ‘Bench toplconole wise stand/sorage cabinet Bench tplconsoe wide stand/trage eabinet ‘Cabinet Conainton ‘All welded stainless sed 16GA, Type 308 presse sight ds All welded stains steel T@GA, “Type 304 pes tight design ‘All welded stainless sel TOR, “Type 304 pressure tight design Die oes Sopa ND ‘Non-limmabie ‘Nonsfimmabie ‘Non anmabie ‘HEPA Filter Seal Type: Supply Filte-99.99% EA on 0 microns HEPEX Seal HEPEX Seal, HEPEX Seal Exhaust Fiie.99 99% EM on 0. eros Neoprene, Spinglonded | Neoprene, Springloaded Neoprene, Spinloaded Fumigation per RIHINSF Procedure Yes Yer Yes ‘Stand Series Service Coupling (8 inch NPT) (ne, Right Sidewalt (Ons, Right Sideall (One, Right Sidewalt {Gas ValvlService Coupling (/8inch NPT) One, Right Siewall (Ons, Right Sidewall One, Right Sidewal Duplex Out ‘One, Backwall Cener__| Two, Bacall ‘Two, Backval ‘Optional Savices (Gas Cocks 38° NPT Upto3 ea Siew Upto 3 ea. Sidewall Upto3 ea Sidewal ‘Remote Conzolled Vaiest® Upto 3 ea, Sidewal Upto 3. Sieall Upto3 ea Siewal ‘Uavoet Light ‘One, Backvall One, Backval One, Beekwal| StandatiCup Sinks LetorRiamt Work Surface | Left or Risk Work Surtace | Ler Riot Work Surtce ‘Cabinet Size Inches (a Height Gully Assembled) 63 (1600) 53,1600) 63 (1600) Height (Minin fr Transpo) eoqiszs) s0(isza) comiszs) wish 4158 4057) 53 5836) 7758972) Dept wth Cont Cee) 32 78 (835), 32780835), 32.78 (835) ‘Work Access Opening inches (rin Standard Opening Height Optional 8,203) oases 03) 10 (25498 209) Standard ino Velocity 105 FPM (33 mi) 108 FPM (33 mv) 103 FPM (33 ms) Work Zone ees Heit 23228) 28 12(78) 2229 with 338 873) 4638 (1178) 70378 0788) | Depa. 23 126599) 212897, 2312 (397) Viewing Window aches an oly closed Fully closed Folly closed Stand isterpered sliding wss 19172 (435) open 1912885) open 19 12.695) open Hinged Tempered Gls optional) 8.203) open (203) & 10/251) opening (203) & 10:25) openings ‘Required Exhaust CFMUICMH Standard Options ‘Gas Tigh (NU518/919) 200040) 33524270 459) 513 (72/410 697) Canopy (NU-918917) 282 479) 438 ca9y370 624) 647 (100,885 (23) Canopy (NU3165) 305,519) $62 (783394 (657, 69 (1139567 868) Plat Das Sate Pesure Eng/Meric 0.0 (aT Sm 005-0777 Sem O060,071272 54m “Heat Reject BTU Per Hour (ann-vened) 1H 1937 vente) 65, ne. ieee ULTOLC Lied ULLAL bed ULL eed Vols, AC 60 #2 us us us Amps Blowe/Lights 7 9 uns ‘Amps: Duplex 3 3 3 Amps: Tota 0 2 lari 12 Power Cord (ne) 16 0A-3 Wie, 158 TGA-3 Wire, 154 2/4 GAS Wie, 20 Sid. /154 Optional ‘Gated Shipping Welsh FA BOAO re 730 A351 Ke Net Weight 425 4/193 500 227 680 bs 08 ie "ISA Configuration dow sot include aceon cunet Remote controled valve handles project though ont fing. Decorative side panels are avallabe to cover plumbing, rated shipping weight does not include weight or szeessoris or options, M01376.0 Operating the NU-440 6.1 Electronic Airflow Control System 61d oMo137 Overview ‘The electronic airflow control system is designed to service the control requirements of the NU-440 Biological Safety Cabinet. The control system is a self-contained microprocessor driven module that will perform the following functions: + Control blower motor via solid state trae. ‘+ Monitor, display and control downflow via dual thermister airflow probe, ‘+ Monitor and display exhaust flow (inflow) via dual thermister airflow probe. ‘+ Alarm setpoints high/low for error conditions (downflow and exhaust flow). Clock display (24 hours) and timer function Control lights via solid state switch, Control outlets via solid state switch. Complete diagnostic functions ‘The NU-440, incorporating the use of the electronic airflow control system, offers improved cabinet performance. The control system uses 2 dual thermister airflow probe in the downflow stream to monitor and control airflow to setpoints. The control system automatically compensates for filter loading, voltage variances and other environmental effects. A second dual thermistor airflow probe in the exhaust airstream monitors for inflow velocity. Both downflow and inflow are displayed on the front panel. The control system also monitors the sliding window position with a micro switch for both window height and window closed positions. ‘The control system through the use of the front panel controls the on/off function of the fluorescent and ultraviolet lights (optional), outlets and blower. ‘The control system also allows contact closure outputs for interaction with HVAC systems to optimize environmental performance. All the above functions are shown in a system block diagram (see Figure 1). x omen RRLOW AR ROE Jerr | __ a roreacom aves [wien eo ecacenacesoon econ weoouon_[/—] ace] — Aan ey Woe cxcees MEADOMTCH wHoeuar paren reuore onsoe —S Jour [fac rena I wee rowen ———[ Fors} a yeavoo Figure 1. System Block Diagram 19omo137 6. 2 Front Panel ‘The control system front panel contains the following functions described in detail (see Drawing BCD- 05165) 6.1.2.1 Blower Switch ‘The blower switch indicates and controls ON/OFF power to the blower. 6.1.2.2 Light Switch ‘The light switch indicates and controls ON/OFF power to the fluorescent and optional ultraviolet lights. 6.1.2.3 Outlet Switch ‘The outlet switch indicates and controls ON/OFF power to the outlets. 6.1.2.4 Window Alarm LED ‘The window alarm LED indicates when the sliding window is raised above its proper operating height 6.1.2.5 Arrow Adjustment Keys The arrow adjustment Keys allow user interaction for various functions 6.1.2.6 Airflow Alarm LED The airflow alarm red LED indicates when a downflow or inflow high/low deviation from setpoint occurs in normal run mode. 6.1.2.7 Select Key The select key allows user interaction to select functional display for various other functions. 6.1.2.8 Set Time Key The set time key allows user interaction with the clock/timer as well as various other fictions. 6.1.2.9 Downflow Display ‘The downflow display indicates the present downflow airflow value in its respective unit of measure. 6.1.2.10 Inflow Display The inflow display indicates the present inflow airflow value in its respective unit of measure. 6.1.2.1 Clock/Timer Display ‘The clock/timer display indicates the present time as well as a user interactive timer function, 6.12.12 Audible Alarm Silence ‘The audible alarm silence key allows user interaction to silence any audible alarm for a period of fifteen minutes. After fifteen minutes, ifthe alarm condition still exists, the audible will again sound 20a SS OAS AEST] (cecal an MI on omo137omo137 614 ‘Run Mode Operations ‘The run mode of the control system is very simple and user friendly with all interaction performed on the front panel. Calibration and set up functions as well as the electronics system master reset performed ONLY BY A QUALIFIED TECHNICIAN during the certification or commissioning. Operation of the cabinet is initiated by plugging the power cord into the appropriate line power. In the power OFF condition (cabinet is unplugged), all calibration and running parameters will be stored in the microcomputers EEPROM memory. However, the clock will not maintain the correct time, only remembering the time when the power was turned off. When power is turned on, the downflow and inflow displays will indicate dashes and the clock display will indicate "POWR" and the time last known by the system. To clear the "POWR" indicator, which means a power interruption has occurred, press the [SET TIME] key. ‘The cabinet has a full counter-balanced and removable sliding glass window with two operational features. As the window is raised above its specified operating height, an audible and visual alarm alerts the operator of possible compromised personnel protection. Ifthe window is closed, it will perform an interlock function that will automatically turn off the blower or prevent it from being turned on. The fluorescent light may be tumed on or off in any window position. However, only one light may be turned on ata time. Once the window is at its specified operating height, the blower may be turned on. Once the blower is turned on, the display will continue to indicate dashes for approximately two minutes during a warm up period and the clock will alternate with time and "WM.UP". Also, during the warm up period, an airflow alarm will occur, both audible and visual. The audible may be silenced by entering into a cleaning cycle by pressing the cleaning key. After the warm up period, the displays will actively indicate the current running parameters. If an alarm condition occurs, the clock/timer will indicate the error message as well as the parameter LED will blink. Clock/Timer Operation 6.1.4.1 Time of Day Clock ‘The time of day clock is displayed in the HH.MM and 24 hour format. To enter the correct time of day, perform the following: « Press and hold (SET TIME] until, display blinks "S:TIM" and "00.00". Continue to hold [SET TIME] until the display blinks *S.CLK" and time, then release. © Press the [ ] or [¥] arrows to the correct time. «Press [SET TIME] to enter the corrected time. ‘Tho time of day clock may also run on @ 12-hour format. This can be altered in the Option Mode by performing the following: ‘+ Press and hold (SET TIME] and (SEL] simultaneously until downflow display indicates “Opt”, exhaust display indicates “OFF” and the clock display indicates “12HR” © Press[_ Jor [J to tum the 12 hour clock on or off. (Note, if 12-hour clock operation is desired, select YON"). # Press [SET TIME] to enter desired operation, # Press and hold [SET TIME] and [SEL} simultaneously until downflow and exhaust display indicates dashes “— The 12-hour clock uses decimal points for various points of information. The center decimal point “XX.XX” blinks elapsed time in seconds. The far right decimal point “XX.XX." indicates PM time of day. No far right decimal point indicates AM time of day.omo137 6.1.42 Timer ‘The timer function is available as a second function to the time of day clock. The timer can be set for an elapsed time of up to 12 hours and 00 minutes. WHEN THE TIMER ELAPSES TO ZERO, IT WILL SOUND THE AUDIBLE ALARM FOR 10 SECONDS. To set the timer, perform the following: + Press [SET TIME], display blinks "S.TIM" and "00.00". « Press the [ ] or [J arrows to the desired time. «Press [SET TIME] to start the timer. The timer will display MM.SS for times less than one hour and HH.MM for times in excess of ‘one hour. To view the clock when the timer is running, simply press the [SEL] key. The time of day will be displayed for a period of S seconds, then return to the timer, To reset timer, press the [SET TIME} key Ifthe timer is used when the ultraviolet light is turned on, the timer will act as an ultraviolet timer and run the ultraviolet light for the length of time set om the timer. 6.1.4.3 User Mode The user mode allows the cabinet user to select the operation of the following parameters. 12,HR - 12 or 24 hour clock (on/off) UV.SY - Ultraviolet light sync witimer (on/off) UV.TM - Ultraviolet light time limit (minutes - hours/0.00) UV.RT - Ultraviolet light run time (hours/0000) Default conditions printed in bold ‘To enter into the user mode, perform the following: * Press and hold (SEL] until downflow display indicates "USr", exhaust display indicates "off" and the clock display indicates "12.HR" ‘The parameters may be altered using the and W arrows to their desired condition. Pressing [SEL] will advance through the above parameters in a round-robin fashion. Once the parameter is selected and altered to the desired state, pressing [SET TIME] will enter the desired condition into memory. To exit the user mode, perform the following: ‘+ Press and hold [SET TIME] and [SEL] simultaneously until dowaflow and inflow/exhaust display indicates dashes ".--'. Clock display will indicate "WMLUP". 2362 oMo137 User Mode Parameter Descriptions 124HR - 12 or 24-Hour Clock ‘This parameter allows for the selection of a 12 or 24-hour clock. As the clock display indicates "12.HR", the exhaust display can be toggled “on” for a 12-hour clock and "off" for a 24 hour clock. UVSY - Ultraviolet Light Syne W/Timer This parameter allows for the selection of the ultraviolet light option to run in syne or not in syne withthe timer, As the clack display indicates "UV.SY’, the exhaust display can be toggled on" forthe ultraviolet light to run in syne with the timer and “off” for the ultraviolet light not to run in sync with the timer. UV-TM - Ultraviolet Light Time Limit This parameter allows for the selection of the ultraviolet light timer to limit, to a specified time period, or not limit the length of time once tuned on. As the clock display indicates "UV.TM", the exhaust display can be adjusted for length of time limit. When the exhaust display indicates "0.00", there is no time limit. When a time limit is entered, the display will start with minutes "0,00 - 99,50" and then go to hours "1.40 - 12.00." UV.RT - Ultraviolet Light Run Time This parameter allows the cabinet technician to review how many hours the ultraviolet light has been running. ‘The time is displayed in hours up to 9,999. However, once the time exceeds 7000 hours, a replace UV light message will appear in the clock display "RP.UV". To clear the run time back to zero and replacement message, press and W arrows simultaneously. 6.1.5 Remote Override (optional) ‘The optional feature remote override is used to remotely control the operation of the cabinet. A typical application would be in a Bio Safety Level three facility that had a room exhaust system failure. ‘The failure mode could signal the remote override contacts to close and not allow any usage of the cabinet. Once the remote override contacts are closed, the intemal blower and fluorescent lights will be tumed off and remain inoperable, If an exhaust motorized air-tight butterfly valve (NU-951-012) is present, the valve will close to seal the exhaust system. ‘The clock display will blink “RM.OV” and the time of day. Once the remote override contacts are broken, normal operation will resume. Operating Guidelines The intent herein is to present general operational guidelines that will sid in the use of the Laminar Flow Biological Safety Cabinet (LFBSC) to control airborne contaminants of low to moderate risk as stated in Technical Report No. FPS 56500000001 prepared by Dow Chemical U.S.A. for the National Cancer Institute, May 1, 1972. Procedure protocols defined in terms of the barrier or control concepts unique to LFBSC must be developed in order to obtain a maximum potential for safety and protection. The pre-planning necessary to develop these protocols is based on several fundamental considerations, each of which will contribute to optimum benefits from the equipment: Know your "safe working area” Minimize disruption of “air curtain" Minimize room activity Utilize unidirectional airflow Employ aseptic techniques 24624 6.2.2 Note: 623 624 oMo137 Know Your "Safe Working Area" ‘The LFBSC safe working area is basically the worktray or depressed area. All work should be performed on or above the worktray. The area on or above the front grill is a non-safe working area. Bose _ Sc FRONT Minimize Penetration of "Air Curtain" ‘The minimum number of items necessary should be placed into the cabinet to prevent overloading, but the work should also be planned to minimize the number of times an operator's hands and arms must center and leave the air curtain at the open face. The ideal situation is to have everything needed for the complete procedure placed in the hood before starting, so that nothing need pass in or out through the air barrier atthe face until the procedure is completed. This is especially important in working with moderate risk agents. Unnecessary raising of the hands inside the cabinet above the level of the work opening should be avoided. This presents an inclined plane from hands to elbows along which the downflow of air may run to, and possibly out, the open face. When working with agents of lower risk, itis not as important for all materials to be placed in the cabinet before starting, or for the procedure to be completely finished before materials are removed. Also, the time period for a unit may be continued over a more extended period during which entries and withdrawals from the cabinet may be made, ‘Minimize Room Activity Activity in the room itself should be held to a minimum, Unnecessary activity may create disruptive air currents as well as interfere with the work of the operator. A person walking past the front of a cabinet can cause draft velocities up to 175 fpm (.89 mis), which are sufficient to disrupt the air balance of the laminar flow unit. Utilize Unidirectional Air Flow ‘The operator must keep two important facts in mind: (1) The air, as supplied to the work area through filters from the top, is contaminant free and (2) Airborne contamination generated in the work area is ‘controlled by the unidirectional flow of parallel air streams in a top-to-bottom direction. A solid object placed in a laminar air stream will disrupt the parallel flow and consequently, the capability of controlling lateral movement of airbome particulates. A coue of turbulence extends below the object and laminarity of the air stream is not regained until a point is reached downstream, approximately equal to three to six times the diameter of the object. Within the parameters of this cone, particles may be carried laterally by multidirectional eddy currents. Transfer of viable materials and manipulations which may generate aerosols should not be performed above sterile or uninoculated ‘materials. Items should be localized on the work surface in "clean" and "dirty" groups. 2563 om0137 6.2.5 ‘Employ Aseptic Technique The operator must not assume an attitude of “let the cabinet do it" when performing procedures LFBSC. Properly balanced and properly used cabinets will do an excellent job of controlling airborne contamination and containing viable agents, but the cabinet will not eliminate contact transmission of contamination. Normal laboratory contamination control procedures and basic aseptic techniques are necessary to obtain maximum benefit from the cabinet. For example, open bottle, tube o flask mounts should be kept as parallel as possible to the downflow to minimize capture of chance particulates. This precaution is merely an extension of good aseptic technique as practiced on open bench tops. The good laboratory practices designed to minimize creation and/or release of aerosols to the environment should not be discontinued. ina Items of equipment in direct contact with the etiologic agent must remain in the cabinet until enclosed or until surface-decontaminated. Trays of discard pipettes must be covered before removal from the cabinet (aluminum foil may substitute for fabricated covers). If an accident occurs which spills or splatters suspensions of etiologic agent around the work area, all surfaces and items in the cabinet must be surface-decontaminated before being removed. Applying a bumer flame to flask and tube necks when mating surfaces of sterile assemblies is a conventional method of minimizing chance contamination. However, the efficiency of this operation is usually related to the removal of airborne contamination occurring while the item is uncovered. If the ‘manipulation is carried out in an environment free of airborne particulates, then the need for the flaming, ‘operation is essentially removed. This is one of the additional advantages of the LFBSC - use of the gas ‘burner is seldom necessary. The gas burner flame in one of these units not only contributes significantly to the heat build-up, italso disrupts the laminar air streams which must be maintained for maximum efficiency. IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS, It should also be only used from the center of the work surface to the right rear where resulting air turbulence will have a minimal effect. DO NOT USE GAS BURNER ON THE LEFT OF ‘THE WORK SURFACE DUE TO ITS INFLUENCE ON THE ELECTRONIC AIRFLOW CONTROL SYSTEM. If cabinet air is inadvertently tumed off, the flame could damage the HEPA filters, Operating Sequence 63.1 Note: Start Up ‘Turn on cabinet blower and lights, check air intake and exhaust portals of the cabinet to make sure they are unobstructed. The electronic airflow control system will automatically control airflows to specified setpoints. ‘Some cabinets are equipped with ultraviolet (UV) lights. Good procedure includes the decontamination or wipedown of cabinet surfaces with chemical disinfectant before work commences. This practice climinates the need for UV lights, whose primary utility in this application is inactivation of surface contamination since the filters effectively remove all aitbome contaminants, UV lights, therefore, are not recommended in the LEBSC. Allow blowers to operate for a minimum of 15 minutes before aseptic manipulations are begun in the cabinet. If the filtered air exhausted from the unit is discharged into the room, as in some installations, an additional advantage is obtained from purification (filtration) of the room air circulated through the equipment. Because of this characteristic contributing to the quality of the laboratory environment, some owners of LEBSC leave them in operation beyond the time of actual use632 63.3 63.4 63.5 63.6 63.7 M0137 Wipedown ‘The interior surfaces of the workspace should next be disinfected (see cleaning procedures) by wiping them thoroughly with 70% alcohol or similar non-corrosive anti microbial agents, USE OF CHLORINATED OR HALOGEN MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL. Materials & Equipment ‘The apparatus and materials should next be placed into the cabinet. Care must be exercised that no items bee placed over the front intake grills, Materials should be arranged so that clean, dirty (used), and virus tmaterials are well separated. Passage of contaminated materials over uninoculated cultures or clean ‘lassware should be avoided and transfer of viable materials should be performed as deeply into the cabinet (away from open face) as possible. Air Purge Additional purging of the workspace without user activity should be allowed for 2-3 minutes after materials and apparatus have been placed in it. This will rid the area of all "loose" contamination that may have been introduced with the items. Perform Work The work can now be performed. The technician performing the work is encouraged to wear @ long- sleeved gown with knit cuffs and rubber gloves. This will minimize the shedding of skin flora into the work area and concurrently protect the hands and arms from viable agent contamination, At a minimum, the hands and arms should be washed well with germicidal soap before and after work in the cabinet. For the preparation of antineoplastic drugs, the following procedures summarize those contained in OSHA Instruction PUB 8-1-1, "Work Practice Guidelines for Personnel Dealing with Cytotoxic (Antineoplastic) Drugs.” The above document should be thoroughily studied/reviewed prior to drug preparation in the cabinet. a. Asterile plastic-backed absorbent drape should be placed on the work surface during mixing procedures. The drape should be exchanged whenever significant spillage oceurs, or at the end of each production sequence. Vials should be vented with a filter needle to eliminate internal pressure or vacuum, ©. Before opening ampoules, care should be taken to insure that no liquid remains in the tip of the ampoule, A sterile gauze sponge should be wrapped around the neck of the ampoule while opening. 4. Final drug measurement should be performed prior to removing the needle from the stopper of the vial ©. Anon-splash collection vessel should be available in the biological safety cabinet to discard excess drug solutions. ‘Terminal Purging & Wipedown Following completion of work, allow the cabinet to run for 2-3 minute period without personnel activity to purge the unit. The decontamination of the interior surfaces should be repeated after removal of all materials, cultures, apparatus, etc. A careful check of grills and diffuser grids should be made for spilled or splashed nutrients which may support fungus growth and resulting spore liberation that contaminates the protected work environment. Paper Cateh/Prefilter ‘A permanent paper catch is installed behind the rear divider panel of the work zone. This area forms the return air path to the motor/blower; and if the airflow is blocked, it could seriously affect the performance of the cabinet. Therefore, THE PAPER CATCH SHOULD BE CHECKED AND ‘CLEANED ON NO LESS THAN A WEEKLY BASIS; daily basis if procedures dictate the use of paper products. Any paper removed must be properly disposed of as Contaminated Hazardous Waste. ‘The above procedures also applies to all units configured with a prefilter. 2763.8 Shut Down Tarn off blowers and lights. Do not use eabinet as a depository for excess lab equipment during periods of non-operation. If antineoplastic agents are being prepared in the cabinet, it is recommended to let the cabinet run 24 hours per day. This lessens the possibility that contaminants may escape. 64 Ergonomics Ergonomics, the study or accommodation of work practices is extremely important for proper cabinet usage and user health and safety. An evaluation of normal work practices should be performed with each user when working in @ cabinet, Evaluation criteria should be at a minimum: 1, Proper user posture b. Effective workzone layout for work practice ¢. Vision or sightlines For each of the above evaluation criterion, several aids may be supplied to accommodate the user. Ergonomic chair - A six-way articulating seat and back control for personalized adjustment to assure proper user posture. Be sure feet are resting on the floor, chair foot support or foot rest. Also be sure back is fully supported with proper chair adjustments. Forearm/armrest support - The cabinet is provided with a forearm support on the work access opening, Periodic mini-breaks during work practice should be taken resting forearm to avoid stress and fatigue Effective workzone layout - Always prepare your work procedure to minimize reach to avoid neck and shoulder stress and fatigue, Rotating tables are optional to maximum workzone and minimize reach. Vision and sightline - Always prepare your work procedure to eliminate glare and bright reflections on the window. Keep your window clean and sightlines clear to your effect workzone. 65 Cleaning Procedures Cleaning the cabinet is an important function in terms of both containment and sterility. Use the following, procedure to effectively clean or surface disinfect the cabinet workzone surfaces. a b, omo137 Raise the sliding window to a full-open position, if desired. Press the audible alarm silence or cleaning key on the front control panel to silence the audible alarm during the cleaning process. Apply appropriate disinfecting solution to cabinet surfaces. Most surface disinfectants require a specific contact time depending upon the microbiological agents used within the cabinet. CONSULT APPROPRIATE DISINFECTANT DOCUMENTATION FOR PROPER APPLICATION AND SAFETY PRECAUTIONS. NOTE:DISINFECTANTS THAT USE CHLORIDES AND HALOGENS WILL CAUSE. DAMAGE TO THE STAINLESS STEEL SURFACES IF LEFT ON FOR LONG PERIODS OF TIME. After the specified contact time, wipe up excess disinfectant, IF THE DISINFECTANT USED CONTAINS CHLORIDES OR HALOGENS, RE-WIPE ALL SURFACES WITH 70% ALCOHOL OR SIMILAR NON-CORROSIVE ANTI-MICROBIAL AGENT TO PREVENT DAMAGE TO STAINLESS STEEL SURFACES.66 ' pvaitable fom Lab Safety Supply, Janesville, WI oMo137 Antineoplastic Decontamination Procedures This procedure should be executed following 2 spillage and/or periodic maintenance, testing or relocation of the cabinet. In addition, ‘the cabinet is being relocated or tuned off for an extended period, the work access opening and exhaust HIEPA filter opening should be sealed with plastic. 6.6.1 6.6.2 Note: 6.6.3 Preparation Prior to beginning decontamination activity, personnel should put on a Tyvek! isolation gown, 2 pair of vinyl gloves and a full-faced HEPA filtered respirator. All protective garments should be contained in 4 mil plastic bags and labeled for disposal as chemotherapy waste after completion of the procedure, For the purpose of this procedure, the term CLEANING is defined as the operation of wiping down with a cloth wetted with a clean hot (above 60°C) detergent solution, followed by wiping down repeatedly with sterile water to rinse. All cloths shall be contained in 4 mil plastic bags and labeled for disposal as chemotherapy waste. Procedure a. Make sure that the cabinet remains in operational mode with internal blower on. b. Open the hinged or sliding view screen and secure in the full open position. CAUTION: — With the view screen in the full open position, personnel protection is compromised and a full faced HEPA filtered respirator must be worn, © Clean alll readily accessible surfaces of the cabinet. 4. Remove perforated metal diffuser screen from the underside of the supply HEPA filter and place on the cabinet work tray. Depending on the model, the diffuser screen is secured to the cabinet by #8-32 screws or 1/4" - 20 acom nuts, 3 places. It is purposely a tight fit and is secured to the back wall with projecting threadless studs. ©. Clean both sides of the perforated metal diffuser screen and remove it from the cabinet. f. Lift the cabinet work tray, clean both sides and remove it from the cabinet. & Remove the front perforated grill, place on the cabinet floor and clean both sides, Remove from cabinet fh, Clean work tray supports i. Working from top to bottom, clean all inside surfaces of the cabinet, Take care not to wet the HEPA filter. If liquid has collected in the plenum drain, aspirate it using an TV tubing into an evacuated container. Label the evacuated container for disposal as chemotherapy waste. Clean the plenum drain area and wipe dry. Ifthe cabinet requires maintenance and/or replacement of the HEPA filters, the operation should be halted at this point to allow trained personnel to complete replacement of the HEPA and/or ‘maintenance action required. Assembly Replace front (if removed) grill Replace the work tray and carefully tighten the thumbserews, Replace perforated metal diffuser screen over the underside of the supply HEPA filter. Wipe down all exposed surfaces of the work area with 70% isopropyl alcohol. Prepare for aseptic operat 1368, o other aheratry. industria, o hospital supply dsribulos, 297.0 General Maintenance CAUTION: ATTENTION ACCOMPANY'S POTENTIAL ELECTRICAL INFORMATION OR IMPORTANT HAZARD ONLY QUALIFIED SYMBOL PERSON TO ACCESS 408 7 All maintenance actions on this equipment must be performed by a qualified technician who is familiar with the proper maintenance procedures required for this equipment. This includes both certification as well as repair. 7.1 Decontamination ‘No maintenance should be performed on the interior of the Labgard cabinet (area behind access panels) unless the cabinet has been microbiologically decontaminated, is known to biologically clean, or known to be chemically inert. Surface disinfection is performed as specified in the cleaning procedures. If microbiological decontamination is necessary, use the following procedure: a Remove screws at each upper side of the control center and allow the control center to rotate down, resting on the safety straps. Remove control center by disconnecting safety straps and moving control center to the left off the slip hinges. Remove the front decorative panel via top/front fasteners. Remove left and right window farings via fasteners. Remove armrest via fasteners. Place decontamination equipment inside the work area. Reference decontamination procedure, per NSF Standard 49, Annex G, using the following chart to calculate chemical requirements. Cabinet Size 300 400 600 ‘Cabinet Dimensions | 60x 29-1/8 x 34-3/8 in. | 60x 29-1/8 x 46-378 | 60x 29-1/8x 70-318 (1.52x.739x.873 m) | (1.52x.739x 118m) |_(1.52x.739 x 1.88 m) ‘Cabinet Volume 34.8 cu. f, 46.9 cu. 71.2 cu. R (985 cu. m) (0.328 cum) (2.016 cu. m) Note, the outlets in the work area are energized as long as the cabinet is plugged in and switched on the ‘front panel. Unplug the cabinet before decontamination equipment is plugged into these outlets or nun the decontamination power cords under the front seal area. Use duct tape and plastic to seal the front and exhaust area. CAUTION: BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY LABORATORY EXPOSURE TO DECONTAMINATION GAS. Perform decontamination procedure per NSF Standard 49, Annex G. Ifthe cabinet has been used to prepare antineoplastic drugs, (chemotherapy), or other toxic chemicals, decontamination of the cabinet cannot be accomplished by the above procedure. It is recommended that the following protective measures be taken: omo137 30Gloves Gloves must be worn. Care must be taken not to cut, puncture, or tear the gloves. No one glove material is impervious to all CYTAs; disposable surgical or polyvinyl chloride (PVC) gloves provide substantial but not complete protection. PVC gloves probably are more protective than surgical gloves, but they are stiffer and less tactile. Gloves should be discarded after each use. Gloves should be tucked into the cufis ofthe gown. Double gloving should be considered. Face & Eye Protection A disposable dust and mist respirator and either a plastic face shield (preferred) or chemical splash goggles must be worn, The face shield or goggles should be wiped clean with a suitable tissue and water after each use, Gowns A protective garment must be worn. The garment should be made of lint-free, low-permeability fabric and must have a closed front, long sleeves, and elastic or knit closed cuffs. Tyvek! isolation gowns are one example of an acceptable garment. The garment must be worn outside the work area. Disposable gowns are preferred over reusable, Front-buttoned coats are not recommended. Hair & Shoe Covers Disposable hair and shoe covers should be worn, Motion Slow and deliberate motions are necessary when working in the interior of the cabinet, in order to minimize the generation of particulates lease consult with Nudie, Ine. about any unique contamination problems, Normally, no preventive maintenance is required on the interior of the cabinet (i. the area behind the access panel containing the HEPA filters and motor (blower assembly). All required adjustments in order to maintain proper cabinet airflows are external to the cabinet interior. ‘The motor is lubricated for life and is thermally protected with automatic reset. 7.2 Lamp Replacement, Fluorescent The two (T8) fluorescent lamps are cool white, rapid start and placed external to the cabinet to aid maintenance ‘and minimize heat build-up within the cabinet. The life rating of the lamp is 9000 hours based on three-hour ‘buming cycles To replace a lamp. itis necessary to remove the lamp assembly. L 2. First, switch Cabinet Light Switch off, Second, remove the screws at each upper side of the Control Center and allow the Control Center to rotate down, resting on the safety straps. ‘The lamp is now directly exposed for replacement. ‘The lamp bulb is removed by displacing the lamp to one side against the compressible lamp holder and lifting out the lamp. Reverse the procedure to reinstall the lamp assembly being careful not to pinch the safety straps, cable or tubing during closure of the control center. 1 aval fom Lab Suey Supply, Janie, WI S547-1368, oor boeon indus or hosp supply dsibuor oMo137 3113 0M0137, HEPA Filter/Motor Replacement (Drawing BCD-05153) ‘The HEPA Filters under normal usage and barring an accident (a puncture), do not need replacement until the efflux velocity cannot be maintained or the access inflow velocity cannot be maintained at 100 LFPM (51 m/s)(min.). This may permit the average downflow velocity to be as low as 65 LFPM (.32 ns) as long as no point falls below 20 percent of the average downflow velocity ‘The HEPA Filters should not be replaced until the entire cabinet has been decontaminated or known to be biologically “clean”. 713A Procedure (see Drawing BCD-05153) CAUTION: Disconnect electrical power from the unit before attempting any maintenance action. Step 1: Remove screws at each upper side of the control center and allow the control center to rotate down, resting on the safety straps. Second, remove the front decorative panel which is held into position by (3) knurled nuts on the top edge and (6) knurled screws on the front. Step 2: Place sliding window into lowest position and remove front filter panel, which is held into position by Phillip pan head screws. Once the screws are removed, the panel is held into position by smooth Weldstuds located on the top commer of the front filter panel. Use the window stop brackets as handles to remove the panel, CAUTION: Screws are used in lieu of acorn nuts, and lockwashers. The screws have O-rings and should be replaced if damaged or badly deformed. ‘The interior of the cabinet is now fully exposed for replacement of the filters and/or motor/blower. Step 3: Filter Removal It is not always necessary to replace both the supply and exhaust filters atthe same time. If during the course of certifications, the downflow falls off while the exhaust increases (i. greater than 110 LFPM)(.56 res), the supply filter is "loading" faster then the exhaust filter, and only the supply filter may need replacement. The opposite might also happen depending upon many factors. a Toremove the supply filter: 1. First, remove the HEPEX/choke tray band clamp between the supply HEPEX and the exhaust filter choke tray. Separate the plenum from the choke tray. Note: Double sticky back gasket is used to hold the plenum to the choke tray and should be replaced when reassembling. 2, Remove the 2 supply filter hold-down clips (1 each side) holding the filter down, 3. Remove the HEPEX pressure plenum from the blower assembly. The HEPEX is clamped to the blower assembly via a band clamp. 4. Carefully remove the supply fiter and HEPEX. The HEPEX can be folded neatly to seal the contaminated side of the HEPA. Direct exposure should be avoided. CAUTION: Dispose of spent HEPA filters properly. Avoid direct contact to "dirty side” of the filters. Label toxie waste.14 ‘To remove the Exhaust HEPA: 1. Relax the exhaust filter seal loading mechanism by tuming the four threaded bolts counterclockwise until one can see a definite release of the loading springs. 2. Pull the exhaust choke tray free and remove the filter. It is not necessary to remove the tray, although itis free to move forward several inches, if necessary, to free the HEPA filter. Step 4: Filter Installation ‘When installing new filters, USE ONLY FILTERS OF THE SAME RATED FLOW AND SIZE AS ORIGINALLY INSTALLED. It is recommended that a new HEPEX/Supply filter be installed since the HEPEX is factory installed to the filter. However, field installation kits are available separately from the filter. To install the supply filter, simply reverse the procedure outlines in Step 32, above. Note: Be sure to open the choke plate fully before inserting the filter into the tray. This will assist in adjusting the airflow. To install the exhaust filter, apply a thin layer of silicone grease to the top and bottom gaskets of the filter and carefully insert into the exhaust choke tray. Position the filter frame within the outside walls of the exhaust opening on the top of the hood. Tighten the spring loaded bolts, 4 places, depressing the gasket material by 1/8 inch (3mm). Step 5: Motor/Blower Assembly Removal a, It is recommended that the motor/blower to be removed as a single unit, To remove, disconnect electrical connections to the motor, remove the HEPEX pressure plenum and unbolt the ‘motor/blower assembly from the roof of the cabinet (4 places). Always inspect the rubber isolation motor mounts and replace those that are cracked or visibly show stress. Replace the motor exactly as originally installed in the blower housing, paying particular attention to the correct electrical connections (see Electrical Schematic). Re-install the new motor/blower assembly. Sliding Window Replacement & Adjustment The sliding window replacement is accomplished by removing the front decorative panel, control center and window el je assemblies (see BCD-05144). The sliding window adjustment may be required due to everyday use over the life of the cabinet. The left window glide is stationary since it contains the microswitches that monitor window height. The right window glide is adjustable by a set screw and tension screw method (see Drawing BCD-05144). When adjusting the sliding window, be sure to verify proper microswitch operation. If the sliding window is too loose, the sliding window will not properly activate the microswitches, thus causing. potential operational malfunctions to occur. M0137 3315 M0137, Airflow Control System Setup and Calibration TSA General ‘The operation of the NU-440 cabinet requires that the setup and calibration procedures be performed in order to certify or commission the cabinet for usage. The setup and calibration procedures PERFORMED ONLY BY THE CABINET CERTIFIER ensue that cabinets setpoints are verified and that the airflow monitor probes are calibrated to read the correct values. Access to the setup and calibration functions requires the use of the hidden key to limit accessibility. Please note, the current Prom chip firmware revision status is displayed upon power-up in the clock display. The revision status of the operating system could impact the calibration process and/or features of the system. Ifdesired, please check with NuAire Technical Service for the latest revision level PLEASE NOTE, SOFTWARE REV. 4.0 OR HIGHER HAS THE CAPABILITY TO LOCK OUT ACCESS TO THE CALIBRATION AND OPTION MODES. This is performed by using the configuration switch, position 6. If the switch is on, access to the calibration and option mode is denied. Ifthe switch is off, access is possible. / (00D \ CO SWITCH ALS [=] = (alae, (ALLL Entry into the setup and calibration functions requires pressing the hidden key for a period of 3 seconds. Confirmation of being in the setup/calibration function will be indicated on the downflow display with the word "CAL", the clock display will indicate "2". To access the setup/ealibration function, perform the following: + Make sure the configuration switch position 6 on the main control board is off. * Press and hold the green flag, downflow display will indicate "CAL" and clock display will indicate "A2", To exit setup/calibration function, press and hold the green flag until the clock display indicates time of ay and the downflow and inflow displays indicate dashes. If necessary, change the configuration switch position 6 on to limit calibration access.oMo137 752 Setpoint and Alarm Limits ‘The setpoint and alarm limits can be verified or altered in the control system. Typically however, these default values are factory set and don't require alteration. ‘The setpoint establishes the airflow values that are to be maintained. The high and low limits establish the alarm boundaries from the nominal setpoint. ‘The default values have been established based upon the performance specifications and cabinet component tolerances. To verify or alter any of these values, simply enter into the setup/calibration function and press the [SEL] key repeatedly until the desired display has the green LED next to the display illuminated. Note, the blower switch must be off to access the setpoint and alarm limits, 752.1 Downflow Setpoint (Default Value, 70 FPM) ‘+ Press [SEL] to indicate blue LED next to downflow display ‘+ Press [SET TIME}. clock display will blink "SET.P" and the setpoint value. * Press [J or [W] arrows to the desired setpoint ‘+ Press [SET TIME] to enter desired value. 7.52.2 Downflow Upper Alarm Limit Default Value, 76 FPM) + Press [SEL] to indicate blue LED next to downflow display. © Press [SET TIME] and [ }, clock display will blink "HILLM" and the high limit value. © Press [ ]or[W] arrows to the desired high limit value. + Press [SET TIME] to enter desired value. 7.82.3 Downflow Lower Alarm Limit (Default Value, 64 FPM) Press [SEL] to indicate LED next to downflow display. Press [SET TIME] and [W}, clock display will blink "LO.LM" and the low limit value Press [_ ] or [W] arrows to the desired low limit value. Press [SET TIME] to enter desired value, 7824 Inflow Setpoint (Default Value, 105 FPM) + Press [SEL] to indicate LED next to inflow display «= Press (SET TIME] clock display will blink "SETP" and the setpoint value © Press[_ ] or {W] arrows to the desired setpoint. + Press [SET TIME] to enter desired value. 7.52.5 Inflow Upper Alarm Limit (Default Value, 120 FPM) + Press [SEL] to indicate LED next to inflow display. « Press [SET TIME] and [_}, clock display will blink "HILLM" and the high limit value. © Press ] or [W] arrows to the desired high limit value. + Press [SET TIME] to enter desired value, 7.52.6 Inflow Lower Alarm Limit (Default Value, 90 FPM) « Press [SEL] to indicate LED next to inflow display. ‘© Press [SET TIME] and [W}, clock display will blink "LO.LM" and the low limit value. ‘© Press[_ ] or (¥] arrows to the desired low limit value. « Press [SET TIME] to enter desired value. 35783 Airflow Calibration ‘The NU-440 Airflow Calibration Consists of adjustments to balance the airflow within the cabinet and the calibration of the airflow monitor probes. THUS WORK SHOULD BE DONE ONLY BY A QUALIFIED TECHNICIAN WHO CAN MEASURE THE AIRFLOW FROM THE FILTERS ‘WITH A SUITABLE VELOMETER. NuAire provides two adjustments to balance the airflow within the cabinet, These are: a blower speed adjustment vie control system », exhaust filter choke ‘The blower speed control system adjusts the cabinet’s total volume of airflow, while the choke adjusts or balances the exhaust airflow as well as makes up for filter resistance tolerances. Since it has been NuAire's experience that the filters may not "load" evenly, both adjustments are necessary for proper cabinet performance. Motor/blower voltage should also be monitored and recorded upon final calibration. The motor voltage may be monitored using a digital voltmeter in the VAC mode. ‘The two test points to measure motor voltage are the blower out (Hot) position on the main control board and any position in terminal block 2 (Neutral(see sketch below). Ca > Fr MOTOR VOLTAGE TEST POINTS ‘The cabinet is considered to be certifiable ifthe following airflow measurements are present: a. Downflow Average: 70 LEPM £5 LFPM (35 ms +.025 m/s) b. Inflow Average: 105 LFPM-+5 LFPM (.53 m/s +.025 m/s) using the direct inflow measurement method or constricted 3 inch (76mm) high access opening measurement method Both values are published in the NSF or Nudire listing omo137 36M0137 7.83.1 Downflow Calibration Step Step 3: Step 5: Step 6: Access setup/ealibration function, If already accessed, proceed to step 2. Ifnot, perform the following Press and hold the green flag until downflow display indicates "CAL" and clock display indicates "A2" Press [SEL] to indicate LED next to clock display. Press [SET TIME] and [_} simultaneously, clock display will blink "BLOW" and a blower duty cycle percentage "XXX.X" Press blower switeh to on Allow blower to run for one minute or until downflow and inflow displays become steady. Place a velometer in the cabinet workzone on the horizontal plane defined by the bottom edge of the viewing window. Spot check several points on the recommended downflow velocity test grid found in table 7.0. Press {Jor [¥] arrows to adjust blower speed. The objective of this spot check is to obtain the desired downflow average velocity as close as possible to the stated goal of 70 LFPM (.35 m/s). DON'T SPEND MORE THAN 5 MINUTES SPOT CHECKING. FINAL ADJUSTMENTS WILL BE MADE IN THE FOLLOWING STEPS. Press [SEL] to indicate LED next to downflow display (blower should remain on). Press [SET TIME], clock display will indicate "CAL.P". In the "CAL.P* mode, the system will automatically control the blower to maintain the downflow monitor probe value as indicated when the [SET TIME] key was pressed. ‘Now, measure the average downflow velocity over the entire ‘workzone using the recommended downflow velocity test grid (02 Table 7.0) Press [Jor] arows to the average downflow velocity just found. Press [SET TIME] to enter the average downflow velocity value. ‘Now the downflow monitor probe has been calibrated to the actual ‘measured average downflow velocity. The cabinet will now control to the downflow setpoint 37omo137 7.5.3.2 Inflow Calibration Note: INFLOW CALIBRATION MUST BE MADE IMMEDIATELY FOLLOWING DOWNFLOW CALIBRATION. THIS ASSURES THE CORRECT BALANCE OF DOWNFLOW TO INFLOW AS RELATED TO THE AIRFLOW MONITOR PROBE CALIBRATIONS. Stepl: Step 2: Note: Step 3: Press [SEL] to indicate LED next to inflow display (blower should remain on). Press [SET TIME], clock display will indicate "CAL.P". ‘Again, in the "CAL.P" mode, the system will automatically control the blower to maintain the downflow velocity to its setpoint. Now, measure the inflow velocity using the recommended procedure found in Table 7.0. Ifnecessary, adjust the exhaust filter choke located under the front decorative panel, to achieve the correct average inflow velocity within the stated range of 105 & 5 LFPM (.53 + .025 nvs). IF THE AVERAGE INFLOW VELOCITY IS ON THE OUTER EDGE OF THE RANGE (LE. 100-101 FPM)(51 M/S) OR 109-110 FPM (.56 M/S). IT WOULD BE DESIRABLE TO MOVE IT CLOSER TO THE SETPOINT BECAUSE THE INFLOW MONITOR PROBE ONLY FOLLOWS THE EXHAUST AIRFLOW OF THE BLOWER SYSTEM. There is no automatic exhaust control for the inflow to setpoint. If the inflow velocity is calibrated at the outer edge of the range, a greater chance for alarm conditions would exist due to its closer proximity tothe alarm limits, ‘The choke plate adjustment requires a standard blade screwdriver. To adjust, loosen the liquid-tight fitting around the choke adjustment shaft, While monitoring the exhaust flow to check position, turning the choke adjustment shaft clockwise will open the choke while turning counter-clockwise closes the choke, Press [_ ] or [W] arrows to the average inflow velocity just found. Press [SET TIME) to enter the average inflow velocity value. Now, the calibration procedure is complete. If desired, a spot check in the downflow velocity may be performed if felt necessary. Replace the exhaust HEPA filter protective grill, if removed. If the inflow display changes due to the protective grill being present, re-enter into "CAL-P* mode and set display to correct value as measured. Once entire cabinet has been balanced, tighten liquid-tight fastener around choke adjustment shaft To exit setup/calibration functions. Press and hold the green flag. Upon exit, the downflow and inflow displays will indicate dashes and the clock will display the current time of day. 38154 188 M0137 Electronic System Master Reset A master reset function is available to clear the microprocessor’s non-volatile memory in the event the memory develops an error in operation. These types of errors can be caused by poor electricity and inadvertent operational parameters being exposed to the system. The master reset function should only be performed by a qualified service technician, All setup and calibration data will be lost, the memory reinitialized to the default values and all control functions reset to an initial cabinet power on condition. Ifpossible before the master reset function is performed, it would be preferred to know the operational parameters of the cabinet (i.e. motor/blower voltage, setpoints, airflow data from previous certification). To perform the master reset function, perform the following: ‘« Press and hold the [green flag] until downflow display indicates “CAL” and the clock display indicates “Ar, ‘+ Press green flag to put into calibration mode. + Press and hold [SEL] and {SET TIME} simultaneously, then press the {green flag] until the clock display indicates “M.RST”, * Press [SET TIME] to complete the master reset function, the clock display will indicate a new reset time and normal run mode will be the current status (Note: if it is not desired to proceed with the master reset, press the flag to exit) This completes the master reset function. Now the system is required to be set up and calibrated per Section 9.4. Enter into calibration mode and check setpoint and alarm limits. Continue to airflow calibration and use the previous certification data to set motor volts and to spot check airflows. Then, exit the calibration mode and verify performance in normal run mode. Airflow Display Multiplier ‘The airflow display multiplier is a function that relates the actual measured airflow velocity rate of change to the dispiay velocity rate of change. For the Model NU-440, the multiplier is one. This means that for every unit of change as measured by the airflow probe, the display is changed by the same amount. This function should only be changed by a qualified technician directed by a NuAire ‘Technical Customer Service Technician. To change the airflow display multiplier, enter into calibration function and perform the following: ‘© Press and hold the green flag until the downflow display indicates "CAL" and the clock display indicates "A2" ‘+ Press [SEL] to indicate LED next to the exhaust display. * Press and hold the [SET TIME] [ _ or ¥] arrows simultaneously, clock display will indicate "MULT, the exhaust display will indicate the current airflow display multiplier. © Press the [or J arrows to change the airflow display multiplier to the desired value. + Press [SET TIME] to enter desired value. To exit the calibration function, press and hold the green flag until the clock display indicates time of day and the downflow and inflow displays indicate dashes, 916 1d 18 Filter Integrity Check In order to check filter and filter seal integrity, the HEPA filter media and seals must be direetly accessible to the ‘measuring instrument. ‘The diffuser plate placed below the HEPA to protect the filter during normal usage may be removed as follows: ‘The diffuser is secured to the cabinet shell by #1/4-20 acom nuts located immediately behind the front viewing window. After removing the fasteners, drop the front of the diffuser plate several inches and pull forward gently. Note, that the diffuser is purposely a tight fit- itis secured to the back wall of the cabinet interior by a light push - fit with projecting studs. To avoid the window high alarm during the filter integrity check, itis desirable to enter the calibration mode and tum on the blower. To accomplish this, perform the following; Press and hold the green flag until the downflow display indicates "CAL" and the clock display indicates "A2". Press [SEL] to indicate LED next to the elock display. Press [SET TIME] and [ ] simultaneously, clock display will blink "BLOW" and a blower duty cycle percentage "XXX.X", + Press blower switch to on. Now, the filter integrity check can be performed, after the filter integrity check is complete, Press and hold the agreen flag to exit the calibration mode. Upon exit, the downflow and inflow displays will indicate dashes and the clock will display the current time of day. ‘The exhaust filter is typically more difficult to check, since protective grills, charcoal filters, or exhaust transitions could cover the filter. Access panels are usually provided and should be removed, If an air gap exhaust transition is provided, the air gap must be sealed with duct tape or other suitable means to prevent contaminated air from migrating into the exhaust efflux. All exhaust blowers/fans should be turned off during the check. The exhaust sensor shrond can also be removed for the exhaust filter integrity check. Replace the exhaust sensor shroud immediately after the filter check to assure proper operation. Cabinet Leak Tightness Test ‘The cabinet leak tightness test can be performed by several different methods. However, to perform the test, the window access area and the exhaust HEPA filter area must be sealed to pressurize the cabinet. The window access area may be sealed by a custom plate or plastic and duct tape. The exhaust HEPA filter area may also be sealed by a custom plate or plastic and duct tape. Sealing should be accomplished on the cabinet face and cabinet top to assure a flat sealable surface. Cleantiness Classification Test For Pharmacy Application If this cabinet is going to be used within pharmacy, per USP797, the cabinet must be tested to assure compliance to ISO 14644-1, Cleanrooms and Associated Controlled Environments, Part 1: of the Air Cleanliness. The cleanliness classification testis performed using a particle counter to measure particle counts within the cabinet workzone. Turn on cabinet and let warm up for several minutes. Turn on particle counter and flush out sample tubing line to remove latent particles. Set the particle counter to measure 0.5 micron or larger particles at the appropriate measuring rate. ‘Take 5 test points in 1-minute intervals on a grid, in @ horizontal plane as measured approximately 6-inches above the worksurface, The grid location is designed as the workzone centerpoint and each corner measured 6-inches (152mm) from the inside perimeter. Record the 5 particle count values for each of the test points over the 1-minute sample time. All final count particle concentrations and calculated 95% upper confidence limit shall not exceed 100 particles per cubic feet (ppef) or 3520 particles per cubie meter (ppem). M0137 40Table 7.0 ‘Recommended Measurement Methods for Cabinet Downflow & Inflow Downflow Measurement B. M0137 a. Recommended Instruments: Alnor 8500 or TSI 8355 Thermoanemometer b. Procedure: Supply filter efflux is measured on a grid, in a horizontal plane 4 inches (100mm) above the bottom edge of the window. No reading should be taken closer than 6 inches (152mm) from the in perimeter. ¢.___ Test Data - Inches (mm): 300 | 6 | 11.594 | 17.188 [22.782 | 28375 as) | @9_| a3 | Gon | 20, HOO | 6 | 11.729 | 17.458 | 23.187 | 28.916 | 34.645 | 40.375 152 |_29e)_| c4asy_| | c735)_| eso) | (1026) GOO | 6 | 11.838 | 17.676 | 23.514 | 29.352 | 35.190 | 41.028 | 46.866 | 52.704 | 58.502 | 4375 asa | Gon | as) | G97 | “46 | cao | cro) | (1190) | (1339) | 487) | 0635) 6 52) 11.750 098) 173 (343) ‘Number of Readings: ‘Average Velocity emia ts) d. Acceptance Criteria: 1. Average downflow velocity = 65 to 75 fpm (33 to 38 m/s) 2. Individual readings must be within +20 percent of the average downflow velocity. Inflow Measurement a b Recommended Instrument: Shortridge Flowhood ADM-870 or Alnor 8500 or TSI 8355 Thermoanemometer. Primary Procedure: The primary procedure to determine inflow velocity uses a Direct Inflow Measurement (DIM) Instrument (i.e. Shortridge Flowhood). The DIM Instrument can be used directly on the cabinet with NO CORRECTION FACTORS REQUIRED if operated in the local density default mode, NSF has tested the cabinet and established listed air velocities expressed in local density. The DIM Instrument should be equipped with a flowhood that is as close as possible to the width of the cabinet (ie, NU-440-400 should use 1 x 4 foot flowhood). The DIM Instrument should also be duct taped to the cabinet to prevent any sneak air paths from occurring. ‘The DIM Instrument will read inflow volume (i.e. CFM). Use the window access opening area to calculate inflow velocity. Alternate Procedure: ‘The alternative procedure to determine inflow velocity uses a thermoanemometer in a constricted ‘window access opening of 3 inches (76mm) with the armrest removed. Inflow air velocity is measured in the center of the constricted opening 1-1/2 inches (38mm) above the work access opening on the following specified grid. Use the correction factor table to calculate the inflow velocity. alc. Test Data- Inches (mmm): 1.__ DIM Measurement Taflow Volume f"/min.(m'/s) | Access Opening BG@) | Inflow Velocity /mningas) 2. __Constricted 3 inch (76mm) high access opening measurement - Inches (mm): 300 [a | ei9G [iz 792 | inne [21 set [28080 | 3027s cio) | ay | c29 | wn 660) | 7m) io a) eae ase | eer B5a20 | WH | SUT | ILD | wOHTS a) | am | ci» | @o 6) | 50) | aso) | 6x | cor, SO | BE | 28I6 | eae BATON | NOME | 35108 | 37SEE | 412 | BIW | HI | Saws | TOM | Ga | SETTT go | gon | cin | ain, exo) | 39) | en | 986) | 10s) | 139 | 269) | ase | 7s | 1580) | (1686) ‘Naber of Readings ‘erage Velo of Const Area Tins) Areas Veber Tp X Consticed Aree = Consed aM oreonstited Area rea Volume [ Gonstided Rica Volume Gis or erage Veoaiy co | ‘cess Window Area of 8" or 10" Asses | Window Area Area Veoaiy of or Tpit X Conscion Fasor Tage tow Velooy ie ‘Access Window Ares 4. Acceptance Criteria: 1. Access Opening Inflow Velocity = 100 to 110 fpm (.51 to .56 m/s) Areas/Correction Factors for Calculations zr 3” 0" Constricted | Window | Window | Correction | Correction Window Access Access | Factorfor | Factor for Cab. Size | Access Area | Opening | Opening ‘Window | 10" Window 300 2B 191 - 96 - 400 7. 2.58 322 1.0 Loner ‘600 147) 391 489 96 nogieaa omo137 279 ‘Main Control Board Description & Replacement ‘The main control board consists of two interconnected Printed Circuit Board (PCB) assemblies. The front PCB contains the displays, microprocessor and Programmable Read Only Memory (PROM) components, The back PCB contains the power supply, configuration switch, sensor inputs/outputs and control inputs/outputs components. The mechanical and electrical interconnects for the two PCB's all occur within the assemblies and are fastened together with standoffs and screws. CAUTION: _ Disconnect electrical power from the unit before attempting any maintenance action. ‘The main control board is fastened to the control center with (6) 6-32 studs, lockwashers and nuts. All electrical connections are made with removable terminals and/or Faston connectors except for the motor/blower connector which uses a screw terminal. All AC circuits are fuse protected and when replacement is necessary, USE ONLY FUSES OF SAME TYPE AND RATING FOR PROTECTION AGAINST RISK OF FIRE. ‘The configuration switch, located on the back PCB, has six switches, The first four switches dictate the type of cabinet operation. The fifth switch is not used. The sixth switch is used to prevent access to the calibration and option mode. Listed below, is the configuration switch number with its associated type of cabinet operation function. NOTE, EACH TIME THE CONFIGURATION SWITCHES 1 THROUGH 4 ARE CHANGED, A ‘MASTER RESET MUST BE PERFORMED TO INITIALIZE THE CONTROL SYSTEM TO THE CABINET TYPE/MODEL. This is not true of switch 6. It can be switched at any time to limit access to the calibration and option mode. Configuration Switch Number Sl 2 $3 St SS SG ‘Type of Cabinet off off off, off, off on/off NU-440-3/416 off off off) on-off onloft NU-440-3/4/6(8 XG) off off «= on off off onvoff NU-4301435-400 off off on on-off onloft NU-430/435-400(E)(G) off oon off off off on/off. NU-430/435-600 off on off on off onloft NU-430/435-600(EXG) off oon on off off onal. NU-427-400 off on = on om onoff NU-427-600 on off off off, off onloft NU-427-400(E)(6) on off off, «ono on/off NU-427-600(E)(G) on off on-off foot ‘NU-440-3/4/6(D) en off = on ono nfo ‘NU-~430-400(0) en ono onloft NU-430-600(D) on on-off om onlofT 1NU-441-400(6) Note, that each type of cabinet operation will automatically convert to the correct units of measure (feet per minute, meters per second, ete.). In addition, all the default setpoint values will be automatically updated to their respective values. Please note, the current Prom chip firmware revision status is displayed upon power up in the clock play. The revision status of the operating system could impact the calibration process and/or features of the system. IF desired, please check with NuAire Technical Service for the latest revision level. oMmo137 aFRONT PCB fl | eg | ataiy ccanTeo. BOARD Ged When replacing the main control board, if possible, i's desirable to note the operational parameters of the cabinet (ie. motor/blower voltage, setpoints, airflow data from previous certification). The exhaust and downflow probe calibration boards should be removed and transferred to the replacement main control board. All other connectors can be removed and reattached to the replacement main control as necessary. ‘Note, Perform electronic system master reset to clear the control board’s memory to assure proper cabinet operation. To perform the master reset function, perform the following: ‘+ Make sure the configuration switch position 6 on the main control board is off. Press and hold the [green flag] until downflow display indicates “CAL” and the clock display indicates “A2” Press and hold {SEL} and [SET TIME] simultaneously, then press the [green flag] until the clock display indicates “MRST”, «Press [SET TIME] to complete the master reset function, the clock display will indicate a new reset time and normal run mode will be the current status (Note: if it is not desired to proceed with the master reset, press the flag to exit). This completes the master reset function, Now the system requires to be set up and calibrated per Section 9.4. Enter into calibration mode and check setpoint and alarm limits. Continue to airflow calibration and use the previous certification data to set motor volts and to spot check airflows. Then exit the calibration mode and verify performance in normal run mode. oMo137 “47.40 OMo137 Airflow Probe Description & Replacement ‘THE AIRFLOW PROBE AND ITS ASSOCIATED CALIBRATION BOARD ARE A MATCHED SET and serialized to assure they always remain together. The airflow probes are located in the downflow and ‘exhaust flow airstreams respectively. The airflow probe calibration boards are located on the main control within the control center. ‘The airflow probe function utilizes two thermistors that are provided a constant current source, One thermistor is a reference that uses a very low current source. The other thermistor is the airflow measurer that uses a very high current source. As airflow passes over the thermistors, the airflow removes heat from the airflow measurer thermistor. The loss of heat from the thermistor causes the voltage from the thermistor to increase. This increase subtracted from the reference thermistor output voltage is what directly relates to airflow velocity. A repeatable curve can be generated (voltage vs. airflow velocity). ‘The thermistors used are glass bead coated and can be cleaned by gently using a cotton swab and alcohol. Formaldehyde gas or vapor phased Hydrogen Peroxide has no effect on the airflow probes, however, the formaldehyde/Ammonium bicarbonate residue that remains after a decontamination should be removed from the airflow probe thermistors CAUTION: Disconnect electrical power from the unit before attempting any maintenance action. ‘The airflow probes are removed by tuming the locking ring counterclockwise and gently pulling probe away from connector. To reattach the airflow probe, turn probe in keyed connector until key matches, push in and turn the locking ring clockwise until ring locks. The airflow probe calibration board is removed by unfastening (2) 6/32 serews and removing connector, then gently pull up calibration board until free, To reattach, reverse the above procedure 458.0 Error Indicators, Troubleshooting, Option-Diagnostics, & Airflow Probe 81 Performance Verification ‘Audible alarms and error messages occur for a variety of reasons. Whenever an alarm condition has been present for a period of atleast 10 seconds, the audible alarm/error message will be presented and stay on until the error is cleared, ‘The audible alarm will be on for 30 seconds upon initial alarm condition, then on once every ten seconds. When presented with an error indicator, please perform the following: Step 1 Step 2: Step 3: immediate or daily, use guide below to correct the situation. Error Indicator Troubleshooting Guide Exror Indicator = Window Alarm (clock display indicates "WN.EI") = Airflow Alarm ~ Downflow Display LED (clock display indicates "LO.FL") = Airflow Alarm ~ Downglow Display LED (clock display indicates "HLFL") = Airflow Alarm ~ Inflow Display LED (lock display indicates "LO.FL") = Airflow Alarm = Inflow Display LED (clock display indicates "HLFL") Clock Display Indicates "VOLT" M0137 Indicator Sliding window is above its standard working height or microswitch is not operating properly, Dowaflow airflow fell below its lower limit alarm setpoint. Downflow airflow went above its high alarm setpoint. Inflow airflow fell below its lower limit alarm setpoint. Inflow Airflow went above its high alarm setpoint, ‘The control system runs on 24 VAC, ifthe ‘voltage drops below the acceptable level, the "VOLT" display occurs. 46 NOTE ALL ERROR INDICATORS. When the cabinet is running, any and all red LED's indicate an VERIFY ERROR INDICATORS. Error indicators can be verified by turning the errored function on/off, MONITOR RE-OCCURRENCE OF ERROR INDICATORS. If re-occurrence of the error indicator is Correction Verify standard working height and window microswitch operation Recertify cabinet to proper airflow setpoints, Recertify cabinet to proper airflow setpoints, Check orientation of ‘exhaust sensor shroud. Recertify cabinet to proper airflow setpoints, Cheek orientation of exhaust sensor shroud, Recertify cabinet to proper airflow setpoints. Verify voltage to cabinet. Check control circuit fuse ‘on main control board. Replace main control if above items are properly verified,om0137 Exror Indicator Cabinet fluorescent lights won't tum on. Cabinet Blower Won't Tum On, (downflow display indicates "ERR") (inflow display indicates "LOSt") (clock display indicates "SYNC") (clock display indicates "RM.OV") (clock display indicates "POWR") Cabinet outlets won't turn on, Cabinet ultraviolet light won't turn on. Blower or light fuse continues to blow after replacement, (Clock display indicates "RP.UV") Indicator Indicates a power loss to main control has occurred, Indicates that the remote override is activated, preventing the usage of the cabinet (see Section 9.0 for remote override operation). Indicates a power interruption has occurred Indicates that the UV light needs replacement 47 Corection ‘Check light fuse on main control board. Check fluorescent lamps. Check voltage coming out of main control board to light ballasts. Check ballast. Check blower fuse on main control board. Check voltage coming out ‘of main control board. Check wiring to blower. Check blower motor. (Note, blower motor has internal thermal protector. Let blower motor cool off for a minimum of 30 minutes to assure thermal protector is not open.) Press (set time] key to clear indicator. Check outlet fuse located on main control board. Check voltage coming out of main control board Check stiding window position so that i's fully closed. Check blower/lights fuse on main control board. Check voltage coming out of main control board to ultraviolet light ballast. Check ballast. Check for short on output of fuse. Isolate output of fuse by disconnecting light circuit, blower circuit, ete. to isolate short Replace UV light and clear UV run. time clock (see User Mode, Section 6.1.4.3).82 oMo137 Option - Diagnostics Mode The option mode allows A QUALIFIED TECHNICIAN to configure several different operational parameters ‘The option mode parameters are the following: 82.1 12.HR- 12 or 24-hour clock (on/off) 8.2.2 UV.SY - Ultraviolet light syne witimer (on/off) 82.3 AF.DA- Airflow Display Averaging (on/off) 8.2.4 WN.IN- Window Interlock (on/off) 82.5 CN.AL-Control Alarm (manual control) (on/off) 82.6 ALL - Alarm Silence (on/off) 82.7 MBLO- Motor Blower Lockout (on/off) 8.2.8 UV.TM- Ultraviolet light time limit (minutes - hours/0.00) 8.2.9 EX.UP - Exhaust Update (seconds/30) 82.10, MB.RT - Motor blower run time (hours/0000) 82.11 UV.RT - Ultraviolet light run time (hours/0000) 8.2.12 DIAG - Diagnostic Interactions Note: Default conditions printed in bold. To initiate the option mode, perform the following: ‘© Press and hold [SET TIME] and [SEL] simultaneously until downflow display indicates "Opt", exhaust display indicates “off” and the clock display indicates "12.HR". ‘The parameters may be altered using the and W arrows to their desired condition. Pressing [SEL] will advance through the above parameters in a round-robin fashion. Once the parameter is selected and altered to the desired state, pressing [SET TIME] will enter the desired condition into memory. To exit the option mode, perform the following: Press and hold [SET TIME] and [SEL] simultaneously until downflow and inflow/exhaust display indicates. dashes "—", Clock display will indicate "WM.UP" Option Mode Parameter Descriptions 82.1 12.HR- 12 or 24 Hour Clock This parameter allows for the selection of a 12 or 24-hour clock. As the clock display indicates "12.HR", the exhaust display can be toggled “on for a 12-hour clock and “off” for a 24 hour clock. 8.2.2 UV.SY- Ultraviolet Light Syne W/Timer This parameter allows for the selection of the ultraviolet light option to run in syne or not in syne with the timer. As the clock display indicates "UV.SY", the exhaust display can be toggled "on" for the ultraviolet light to run in syne with the timer and "off" for the ultraviolet light not to run in syne with the timer. 823 ARDA- Airflow Display Averaging This parameter allows for the selection of the airflow display averaging function to operate. As the clock display indicates "AF.DA', the exhaust display can be toggled "on" for airflow averaging to occur and "off" for no airflow averaging. When airflow averaging is "on", the downflow display will always indicate the airflow setpoint (ex. 70 fpm) if the airflow is valid and within its alarm limits. The exhaust display will use the exhaust update averaging function 488.2.4 82.5 82.6 8.2.7 M0137 WN.IN - Window Interlock This parameter allows for the selection of the lower window switch to be interlocked with the ultraviolet light option. As the clock display indicates "WN.IN", the exhaust display can be toggled "ON" for the ultraviolet light option to be interlocked with the sliding window and "off" for the sliding window not to be interlocked. CN.AL - Control/Alarm (Manual Control) ‘This parameter allows ONLY THE CABINET TECHNICIAN to run the cabinet in manual mode, ‘This means with no controls or alarms activated. As the clock display indicates "CN.AL”, the exhaust display can be turned "ON" to run the cabinet in manual mode. When the exhaust display is "OFF", the cabinet will run normally with controls and alarms. If the function is turned on, and out of the option ‘mode and into the run mode, the LED next to the clock display will be on. The downflow and inflow displays will indicate the nominal setpoints. Airflow adjustments can be made in the manual mode by ‘going into the calibration mode and adjusting the blower duty cycle. The blower duty cycle will remain constant in manual mode, To adjust the blower duty cycle in manual mode, perform the following: + Make sure the configuration switch position 6 on the main control board is off. + Press and hold the green flag until downflow display indicates "CAL. and the clock display indicates ae # Press [SEL] to indicate LED next to clock display. Press [SET TIME] and ( _] keys simultaneously, clock display will blink "BLOW" and the blower duty eycle percentage "XXX.X", Press blower switch to on Press [J or [W] keys to desired airflow rate. Press [SEL] to indicate LED next to downtlow display. Press [SET TIME], clock display will indicate "CAL.P" Press [SET TIME] two times to enter airflow value, clock display will still indicate "CAL.P, Press te green flag to exit calibration mode, ALSL - Alarm Silence ‘This parameter allows for the selection of the alarm silence key function. As the clock display indicates "AL.SL", the exhaust display can be turned "ON" for the alarm silence key function to silence all current and future alarms for 15 minutes. When the exhaust display is "OFF", the alarm silence key function will silence current alarms for 2 minutes, Ifa new alarm is present, the audible alarm will again be turned on. MB.LO - Motor Blower Lockout ‘This parameter allows the access to turn the blower on or off to be restricted by the use of a password. ‘As the clock display indicates "MB.LO", the exhaust display can be toggled "on" for password protection ‘or “off” for normal operation. PLEASE NOTE, WHEN TOGGLING THE OPTION "OFF", YOU MUST PRESS [SET TIME] TWO TIMES TO TURN OFF THE OPTION. If the motor blower lockout option is "on", upon exiting the option mode, the default sequence of [ ][SEL][W] keys will allow the user to turn the blower on or off. When the lockout function is used, an audible alarm will be present during the warm-up period. Press the alarm silence key to silence. To customize the access code, ‘perform the following: © Pressand hold { _] key until clock indicates alternate blink code/clock time. © Press the [ ][SEL][W] keys in any three consecutive keys. ie: CUM I (SEL I 1 (Wi SEL] * Press [SET TIME] to enter the new code. 498.2.8 UV-TM.- Ultraviolet Light Time Limit This parameter allows for the selection of the ultraviolet light timer to limit, to a specified time period, or not limit the length of time once turned on. As the clock display indicates "UV.TM’, the exhaust display can be adjusted for length of time limit. When the exhaust display indicates "0.00", there is no time limit. When a time limit is entered, the display will start with minutes "0.00 - 99.50" and then go to hours "1.40. - 12.00." 82.9 EX.UP- Exhaust Update This parameter allows for the selection of time to determine how much exhaust airflow data is averaged before being displayed. The time is displayed in seconds with a programmable range of 1 to 60. 82.10 MB.RI -Motor Blower Run Time This parameter allows the cabinet technician to review how many hours the motor blower has been running. The time is displayed in hours up to 99,999. The display time has two elements (0-9999) and (1000. - 9999.), The decimal point in the second element means multiply the value times (10). To clear the run time back to zero, press and W arrows simultaneously. 8241 UV.RT- Ultraviolet Light Run Time This parameter allows the cabinet technician to review how many hours the ultraviolet light has been running. The time is displayed in hours up to 9,999. However, once the time exceeds 7000 hours, a replace UV light message will appear in the clock display "RP.UV". To clear the run time back to zero ‘and replacement message, press and W arrows simultaneously. 82.12 DIAG - Diagnostic Interactions ‘This parameter allows the cabinet technician to verify all inputs and outputs. This can be a valuable aid for troubleshooting or verifying cabinet performance. The diagnostic mode is entered by the following: + Press [J arrow key, display test will be in process. During this test, each display output will be cycled in order. Visually inspect all the displays to assure proper function. The [SEL] key is used to advance to other diagnostic test modes in a repetitive sequence. Each diagnostic test mode is indicated by the LED to the left of the displays. Digital Output Test Downflow = Analog Output Tests Inflow/Exhaust “ Physical Input Tests Clock/Timer - Digital Output Tests ‘TO EXIT DIAGNOSTICS, PRESS THE GREEN FLAG AND THE DISPLAY WILL RETURN ‘TO THE OPTION MODE. The downflow display will indicate "Opt", the exhaust display indicates “off” and the clock display indicates "12.HR". To exit the option mode, perform the following: ‘+ Press and hold [SET TIME] and [SEL] simultancously until downflow and inflow/exhaust display indicates dashes "—". Clock display will indicate "WM.UP", M0137 508.2.12.1 Analog Output Test ‘The analog output test only has two test items. The analog output to the blower and damper motors. The [SET TIME] key is used to advance between the tests. ‘When the output is accessed, the clock/timer display indicates one of the abbreviated outputs below. The or Y arrow keys increase or decrease the output value. Blow Blower analog output value. ‘The value indicated in the downflow display indicates the current output level (0-255). The blower can be turned on to verify operation and to increase and decrease voltage to blower. Damp Damper analog output value, The value indicated in the downflow display indicates the current output level (0-255). The damper can be opened and closed to verify operation. 8.2.12.2 Physical Input Test ‘The physical input test has eighteen test items. The (SET TIME] key is used to advance between each test. When the inpat is accessed, the clock/timer display indicates one of the abbreviated inputs below. Each test item is checked by exercising the actual device (i.e. switeh on/off). When in the downflow and ‘exhaust flow input, with the blower turned on, the downflow display will indicate the current output level (0-255). The — and W arrow keys raise or lower the blower voltage. DNEL Downflow Analog Input - The value in the exhaust display indicates actual flowrate (fpm). EXEL Exhaust Flow Analog Input - The value in the exhaust display indicates actual flowrate (fpm). DNCT Downflow Digital Count - The value in the display indicates a digital count value of actual flow rate, EX.CT Exhaust Flow Digital Count - The value in the display indicates a digital count value of actual flowrate. WNHL Window High Switch - Monitors high point of sliding window. Switch will close when sliding window is above correct operational height. WNCL Window Closed Switch - Monitors closed point of the sliding window. Switch will close when the sliding window is lowered down to the bottom of the access opening, WNLO ‘Window Low Switch (only used on some 230 Vac models)- Monitors low point of sliding window. Switch will open when sliding window is lowered past the standard opening height FLER Flow Error Switch - A contact closure that allows an external signal to indicate an airflow error. NIGHT ‘Night Setback Switch - A contact closure used with the B1/B2 configuration that closes the exhaust damper when not in use to save energy. RM.OV Remote Override Switch - A contact closure that allows an external signal to shut down, the cabinet operation. V.MON Power Monitor Input - Monitors line voltage to controller. Value indicated is an associated value in line voltage. Any value above 350 is considered acceptable. oMo137 5182.123 M0137 SYNC Power SYNC Rate (eycles per second) - Monitors line power cyele rate, either $0 or 60. sw Configuration Switch Position 1 - Monitors position of switeh ON or OFF. sw2 Configuration Switch Position 2 - Monitors position of switeh ON or OFF. sw3 Configuration Switch Position 3 - Monitors position of switeh ON or OFF. sw4 Configuration Switch Position 4 - Monitors position of switch ON or OFF. sws Configuration Switch Position 5 - Monitors position of switch ON or OFF. sw.6 Configuration Switch Position 6 - Monitors position of switch ON or OFF. Digital Output Testing The digital output test has seven test items. The [SET TIME] key is used to advance between each test. ‘When the output is accessed, the clock/timer display indieates one of the abbreviated outputs below. The and W arrow keys can turn the output tests on and off. AALM W.ALM XRLY ERLY ARLY BEEP S.MEM, Airflow Alarm LED - Turns the red airflow alarm LED on front panel. Window Alarm LED - Turns the red window alarm LED on front panel. Auxiliary Relay - Turns the auxiliary relay contacts on and off. Fan Relay - Turns the fan relay contacts on and off. Alarm Relay - Turns the alarm relay contacts on and off, Audible Alarm - Tums the audible alarm on and off ‘System Memory - Performs an internal check on the system memory. The system memory should only be performed by a qualified service technician. All setup and calibration data will be lost, the memory reinitialized to the default values and all control functions reset to an initial cabinet power on condition. If possible, before the system ‘memory function is performed. It would be preferred to know the operational parameters of the cabinet (ie. motor/blower voltage, setpoints, airflow data from dprevious certification). 3283 omo137 Airflow Probe Performance Verification ‘The individual airflow probes can be checked in diagnostics. In the run mode performance verification, the airflow probe is checked for responsiveness to changing airflow conditions. In the diagnostic mode, the airflow probe is checked for calibration in the raw airflow velocity measurement. Both verifications are used to assure maximum performance of the airflow control system, 83.4 832 ‘Run Mode To check the airflow probe in run mode, first allow the cabinet to operate normally for a minimum of S minutes. Then, place a rolled piece of paper over the downflow probe in the workzone and leave the paper on the probe for at least 2 minutes and then remove, This action will cause the cabinet to go intoa downflow alarm condition. The exhaust airflow reading should increase during this test. However, the downflow reading should go down to dashes on the display “~-". There should also be a noticeable increase in motor/blower noise. It would also be recommended to monitor motor/blower voltage during the test. The motor/blower voltage should be monitored from when the cabinet is running normally. During the test, when the downflow probe is covered, the motor/blower voltage should be steadily increasing to slightly under line voltage. When the downflow probe is uncovered, the motor/blower voltage should decrease and return to its original reading If the motor/blower voltage does not change or does not return to the original voltage, an airflow probe problem could exist. Please consult with NuAire Technical Service. Diagnostic Mode (not required on software rev. 4.0 and higher) ‘To check the airflow probe in diagnostic mode, first use a velometer placed near the airflow probe which determines what the actual airflow is near the probe during the verification process, Then, proceed to access the diagnostic mode by the following procedure: © Enter Option Mode Press and hold [SET TIME] and [SEL] simultaneously until downflow display inc exhaust display indicates "off" and the clock display indicates "12,HR". * Press [SEL] to advance through option mode until "DIAG" appears in display. Press] arrow key, display test will be in proce: Press [SEL] to advance through diagnostics until exhaust display indicates actual flowrate. ‘© Press blower [ON] key and allow blower to warm up for a minimum of 2 minutes. tes "Opt", IN.FL" appears in the clock/time display and the ‘Compare diagnostic value in exhaust display to a velometer measurement next to the downflow airflow probe. Press (SET TIME] to advance display to "EX.FL" and repeat the velometer measurement next to the exhaust airflow probe (Note, the exhaust sensor shroud must be removed for velometer measurement). Record values and exit diagnostics. The measured values should be within + 30 fpm of the diagnostic value. If the measured values are not within the +30 fpm, return airflow probe and calibration board to NuAire for recalibration. Once test is completed, press [SEL] to advance back to display test, then press the hidden key (flag) to exit back into option mode. Then press [SEL] and [SET TIME] simultaneously to exit back into normal run mode. 39.0 OA 92 93 94 Remote Contacts ‘The NU-140 has several contact closures for remote sensing of various functions, Fan Relay The fan relay contacts are dual normally open contact closure outputs, which are activated whenever the blower is tumed on, Contact ratings are 250 VAC maximum at 2 Amps. Alarm Relay ‘The alarm relay contacts are dual normally open contact closure outputs, which are activated whenever an airflow alarm condition occurs. Contact ratings are 250 VAC maximum at 2 Amps. AUX Relay The AUX relay contacts are not defined, Remote Override ‘The remote override contacts are (no power) shorting eontacts only, which when closed, indicates to the control system to shut down the cabinet. The blower would be turned off, an audible alarm would be tured on for several seconds and the clock display will indicate "RM.OV". io o NC No [common Io 2 COMMON B ND a COMMON & NOB. COMMaN 3 NDA. COMMON a jo ° FEE REMOTE OVERRIDE CONTACTS FIGURE 2 MAIN CONTROL BOARD REMOTE CONTACTS oMo137 5410.0 Optional Equipment 1041 omo137 Ultraviolet Light 10.1.1 10.1.2 1013 10.1.4 Ove ‘The germicidal ultraviolet is primarily intended for the destruction of bacteria and other microorganisms in the ait or on directly exposed surfaces. Approximately 95% of the ultraviolet radiation's from germicidal tubes are in the 253.7 manometer region. This is a region in the ultraviolet spectrum which is near the peak of germicidal effectiveness. The exposure necessary fo kill bacteria is the product of time and intensity. High intensities for a short period of time, or low intensities for a longer period are fundamentally equal in lethal dosage on bacteria (disregarding the life cycle of bacteria). ‘The intensity of light falling on a given area is governed by the inverse law; that isthe killing intensity decreases as the distance increases from the tube. ‘The germicidal tube is placed in the cabinet to provide an average intensity of 100 microwatts per square centimeter (for a new tube) falling on a horizontal plane defined by the bottom of the work surface. The minimum requirement per paragraph 5.12 of NSF Standard 49 is 40 microwatts per square centimeter (ref. NSF Std. #49, June, 1976). Operation ‘The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV switch located on the front panel. The sliding window is interlocked to the ultraviolet light so, when the sliding window is raised, the ultraviolet light will turn off. If an operational time duration is known, the timer can be used in conjunction with the ultraviolet light to time out the ultraviolet light operation. This can be accomplished by first turing on the ultraviolet light. Then, set the timer to the desired length of ultraviolet light operation time, Upon timer expiration, the ultraviolet light will turn off Precaution ‘The rays from germicidal tubes may cause a painful but temporary irritation of the eyes and reddening of the skin, if of sufficiently high intensity, or if exposure covers a prolonged period of time. For this reason, one should avoid direct eye and skin exposure to ultraviolet light. If exposure cannot be avoided, it is necessary for personnel to wear eye goggles or face shields, and long sleeve gowns with rubber gloves. ‘Maintenance The output of an ultraviolet light deteriorates with burning age. The useful life of the light is approximately 7000 hours under specific test conditions, If the tube is turned on every day for 12 hours, the tube will last approximately one year. Its recommended that either atime schedule be established or the tube's output be measured periodically and the tube replaced when its output falls below 40 microwatts per square centimeter or exceeds 7000 hours of operation. Lights should be allowed to operate approximately 5 to 10 minutes (longer when the light is in low temperatures) to warm up sufficiently and wiped clean of dust or dirt before reading the output with a meter. Even minute amounts of dust will absorb ultraviolet energy. ‘The light may be cleaned with a lint-free cloth dampened with alcohol or ammonia and water. 55Energies Required to Destroy Some Microorganisms By Ultraviolet Radiations(e) Microwatt Microwatt Mola Spores seconds Protozoa seconds perom/2 per em/2 Penicillium roquefortt 26,400 Paramecium 200,000(a) Penicillium expansum 22,000 Penicillium digitiatum 88,000 Nematode Eggs 40,000(b) Aspergillus glaucus 88,000 Aspergillus flavus 99,000 Algae 22,000(c) Aspergillus niger 330,000, Rhizopus nigricans 220,000 Virus Mucor racemosus A. 35,200 Baceriophage (E. Coli) 6,600 Mucor racemosus B 35,200 Tobacco Masaic 440,000 Oospora lactis 11,000 Infiuenze 3.400¢@) Yeasts Saccharomyces 13,200 ellipsoideus 17,600 Saccharomyces cerevisiae 13,200 Brewers! yeast 6,600 Baker's yeast 8,800 ‘Common yeast cake 13,200 Bacteria Streptococcus lactis 8,800 Strep. hermolyticus (alpha type) 5,500 Staphylococcus aureus 6,600 Staphylococeus albus 3,720 Micrococcus sphaeroides 15,400 Sarcina lutea 26,400 Pseudomonas fluorescens 7,040 Escherichia coli 7,040 Proteus vulgaris, 7,480 Serratia marcescens 6,160 Bacillus subtilis, 11,000 Bacillus subtilis spores 22,000 Spirillum rubrum 6.160 References: (a) Luckiesh, Matthew (1946) Application of Germicidal, Ethyemal and Infrared Energy, D. Van Nostrand 0, New York, New York, pp 253 (b) _ Hollaender (1942) Aerobiology, A.A.A.S. (for 90% inactivation), pp 162 (©) Ellis, C. and Wells, 0.0. (1941) The Chemical Action of Ultraviolet Rays, Reinhold Publishing Corp., pp. 713- 74 (® —Hollaender, A., Oliphant, J.W. (1944) The inactivation effect of monochromatic ultraviolet. Radiation on Influenze Virus (for 90% inactivation) Jour. of Bact. 48, pp. 447-454 (© This table, "Energies Required to Destroy Some Microorganisms by Ultraviolet Radiations" comes from ‘Westinghouse brochure entitled - "Westinghouse Sterilamp Germicidal Ultraviolet Tubes” omo137 3611.0 12 13 14 15 16 wt Electrical/Environmental Requirements Electrical NU-440-300_115V, 60Hz, I Phase, 10 Amps YNU-440-400115V, 60Hz, I Phase, 12 Amps *NU-440-600115V, 60Hz, 1 Phase, 14 Amps *UL/UL-C Listed Operational Performance (for indoor use only) Environment Temperature Range: 60°F-85°F (15°C - 30°C) Environment Humidity: 20% - 60% Relative Hr Environment Altitude 6562 Feet (2000 meters) maximum Light Exposure Standard Fluorescent Lighting @ 150 ft. candles (1614 LUX) maximum intensity. Installation Category: 2.0 Installation category (overvoltage category) defines the level of transient overvoltage which the instrument is designed to withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example, in CAT Il, which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply. Pollution Degree: 2.0 Pollution degree describes the amount of conductive pollution present in the operating environment, Pollution degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation. Chemical Exposure Chemical exposure should be limited to antibacterial materials used for cleaning and disinfecting CHLORINATED AND HALOGEN MATERIALS ARE NOT RECOMMENDED FOR USE ON STAINLESS STEEL SURFACES. Chamber decontamination can be accomplished by paraformaldehyde, vapor phased Hydrogen Peroxide or Ethylene Oxide without degradation of cabinet materials, EMC Performance (classified for light industrial) Conducted Emissions: CISPR 11, Class B & ENS5O11 Radiated Emission: CISPR 11, Class B & ENSSO11 Radiated Immunity ENS0082-1, IEC 801-3, Level 2 ESD Immunity: ENS0082-1, IEC 801-2, Level 2 EFT/BURST Immunity: ENS082-1, IEC 801-4, Level 2 (Note: The EMC performance requirements are generated within the product enclosure, The enclosure will be all metal grounded to earth. In addition, the membrane front panel will also include a ground plane for maximum protection and an electrostatic shield, OMo137 37aa) omo137 38VIEW BAB. 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