Service Manual

EZ read 2000 / UVM 340

Microplate Reader

January 2016 VER 4

Biochrom Ltd, 1020 Cambourne Business Park, Cambourne, Cambridge, CB23 6DW, England
Telephone: +44 (0)1223 423723 Fax: +44 (0)1223 420164
E-mail: [email protected] Support: [email protected]
Contents

1.0 HEALTH AND SAFETY 4

1.1 WARNINGS AND HAZARDS 4
1.2 RATED OPERATING CONDITIONS 5
1.3 CLEANING AND DISINFECTING THE INSTRUMENT 6
1.4 EMERGENCY INSTRUCTIONS 6

2.0 INTRODUCTION 7
2.1 GENERAL DESCRIPTION 7
2.2 TECHNICAL SPECIFICATIONS 7

3.0 THEORY OF OPERATION 8

3.1 INTRODUCTION 8
3.2 MAIN COMPONENTS 8
3.3 OPTICAL SYSTEM 9

4.0 ELECTRONICS 10
4.1 ELECTRONIC BOARD LAYOUT 10
4.2 POWER SUPPLY 11
4.3 BASIC CPU BOARD OPERATION 11
4.4 LIGHT CONTROL BOARD DIAGRAM 12
4.5 PREAMPLIFIER BOARD 12
4.6 INTERFACE BOARD 12

5.0 ADJUSTMENTS 13
5.1 REPLACING THE LAMP 13
5.2 LAMP TRIM PROCEDURE 15
5.3 LAMP VOLTAGE CHECK 18
5.4 WAVELENGTH CALIBRATION CHECK 18
5.5 WAVELENGTH CALIBRATION SETTING 19
5.6 MONOCHROMATOR REPLACEMENT. 20
5.7 TRANSPORT CALIBRATION 22
5.8 POSITIONING SETUP 23
5.9 ADC ADJUSTMENT 30
5.10 TOP COVER ADJUSTMENT 34

6.0 PERFORMANCE VERIFICATION 35

6.1 QC PLATE: 35
6.2 ORANGE TEST PLATE: 35

7.0 ERROR CODES 36

EZ Read 2000 / UVM340 V4.0 2
8.0 TROUBLESHOOTING 37
8.1 BASIC TROUBLESHOOTING 37
8.2 ADVANCED TROUBLESHOOTING 38

9.0 ADVANCED SYSTEM ERRORS 41

9.1 IN RELATION TO CSYS_RUNTIME 41
9.2 IN RELATION TO CSYS_TRPERR 42
9.3 IN RELATION TO CSYS_FLTERR 43
9.4 IN RELATION TO CSYS_ADCLOW 43
9.5 IN RELATION TO CSYS_ADCHIGH 43
9.6 IN RELATION TO CSYS_MVOOMTOL 43
9.7 IN RELATION TO CSYS_MVOOSTAB 43
9.8 IN RELATION TO CSYS_CHTLOW 44
9.9 IN RELATION TO CSYS_WVLCALIB 44
9.10 IN RELATION TO CSYS_VALOORNG 44

10.0 PREVENTATIVE MAINTENANCE 46

10.1 DETAILS 46
10.2 FUNCTIONAL TESTS 46

11.0 SPARE PARTS LIST 47

APPENDIX 1 - DECLARATION OF CONTAMINATION STATUS 48

EZ Read 2000 / UVM340 V4.0 3

1.0 Health and Safety
Please note that only suitably qualified service engineers should open up and work on this equipment
due to the hazards involved.

1.1 Warnings and Hazards

There a number of warning labels and symbols on your instrument. These are there to inform
you a potential danger that exists or where particular caution is required. Before commencing
installation, please take time to familiarise yourself with these symbols and their meaning.
Safety Symbols

Meaning Warning High voltage Biohazard

High Voltage
High voltages exist inside these instruments. Do not remove covers whilst
connected to the mains supply.
Repair, maintenance and service should only be carried out by individuals
trained specifically to work on these instruments and that have been made
aware of the potential hazards.
Trained Users
These instruments are intended to be used by individuals trained in and
familiar with the use of plate readers and washers and their associated
hazards. In the event of a malfunction or hazard occurring, disconnect the
unit from power and isolate for decontamination and /or repair.
Lamp Source
Lamp sources used within the reader units produce a light beam that
passes through the well positions and is normally confined within the
instrument. The unit should not be operated with the covers removed as
prolonged exposure to the beam intensity and potential UV content of the
beam could cause eye damage.
Personal Protective Equipment
There are no bio-hazardous materials within the instrument; however,
this microplate reader may well be used with bio-hazardous samples.
Before using the instrument the user should have in place
decontamination procedures designed to protect laboratory workers
from occupationally acquired infections. A set of suggested
decontamination procedures for microplate readers are provided in this
manual.
 Decontamination. Equipment should be maintained in a clean
state. Equipment returned for repair should include an
appropriate decontamination certificate (refer to website:
http://www.biochrom.co.uk/content/1/65/returns.html)
 Any chemicals used with the microplate reader should be used,
stored and disposed of in accordance with manufacturer’s
guidelines and local safety regulations.

EZ Read 2000 / UVM340 V4.0 4

  Toxic Fumes. Efficient laboratory ventilation must be provided
when working with volatile solvents or toxic substances.
 Waste disposal. Disposal of some solvents and chemicals may be
classed as hazardous waste and must be dealt with in accordance
with local regulatory practice.
 Personal protective equipment including but not limited to gloves,
laboratory coats and safety glasses is recommended when using
this instrument. A local risk assessment should be performed to
determine the extent of required PPE.

Changes or modifications
Any changes or modifications made to the instrument could void the user’s authority to
operate the instrument.
If the instrument is operated in a manner not specified, then the protection provided by the
equipment may be impaired and the instrument warranty withdrawn.

1.2 Rated Operating Conditions

Name EZ Read 2000 / UVM340

+10 to + 35° C (operation)

Ambient temperature
25°C - +50°C (storage)

Consumption 65VA

Dimensions (WxHxL) 27 x 24 x 43cm (10.8 x 9.6 x 17.2 inches)

Frequency range 47 - 63 Hz (auto-sensing)

Height over sea level

up to 2000m
(operation):

Power Requirements
100 to 240 VAC 50/60Hz 65VA

Fuse 3.15A TH 250VAC

Outer lighting influences Precaution, avoid direct sunlight

PC connection USB A to USB B mini cable

Relative humidity 5 to 95%, non-condensing (storage only)

Warming-up time None required

10 kg (No special instructions are required for

Weight
lifting or moving the instrument)

EZ Read 2000 / UVM340 V4.0 5

1.3 Cleaning and Disinfecting the Instrument
For disinfection, authorized trained personnel must operate in a well-ventilated room while wearing
disposable gloves, protective glasses and clothing.

A declaration of decontamination form is given in Appendix 1 (at the end of this document). This
should be completed and signed by the person who decontaminates the equipment.

Details of cleaning procedures are listed below.

To disinfect the instrument:

1. Switch off the reader and disconnect it from the mains power supply and the PC.

2. Carefully wipe off the entire reader with lint-free tissues that have been moistened in a protein
degrading mild detergent or a saline solution.

3. Carefully wipe off the entire reader with non-lint tissues that have been moistened in a 70%
ethanol or a 0.5% bleach solution.

4. Soak non-lint tissues in a 70% ethanol or a 0.5% bleach solution and place onto the plate
transport mechanism and let it soak for ± 30 minutes.

5. If a bleach solution has been used, carefully wipe off the entire reader with non-lint tissues that
have been soaked in water.

6. Dry the reader by wiping it off with non-lint tissues.

Please Note: Before the reader can be returned to base for service, it must be disinfected and a
Declaration of Decontamination Certificate must be completed. This document can be downloaded
from the Biochrom website: http://www.biochrom.co.uk/content/1/65/returns.html
The instrumentation will not be accepted for servicing or return until the above form is completed
fully. Instrumentation that has not been cleaned sufficiently or decontaminated may be subject to
additional charge.

1.4 Emergency Instructions

In the event of an emergency the instrument should be disconnected from the power supply, power
source and PC.

EZ Read 2000 / UVM340 V4.0 6

2.0 Introduction
2.1 General Description
The “UVM 340” is a microprocessor controlled single-channel monochromator driven microplate reader,
designed to measure the optical density of liquid in the wells of 12, 24, 48 and 96 well microplates. In
addition, the spectral characteristic of the liquid can be determined.
Data reduction is performed using external software on a personal or desktop computer.

2.2 Technical Specifications

Measurement range: 0--3.200 O.D

Wavelength range: 340 to 800 nm
Wavelength selection Monochromator, all wavelengths selectable in 1 nm increments
Bandwidth < 3 nm
Accuracy: +/- 0.5% and +/-0.005 OD
Precision: +/- 0.5% and +/-0.005 OD. from 0.000 to 2.5 OD
Plate formats: 12, 24, 48, and 96 well plates
Reading speed: 25 seconds for a 96 well plate
Light source: Tungsten Halogen lamp, 30W
Detection system: Two silicon diodes, one for measurement and one for reference
Single channel optical system with self-check and automatic
Measurement system:
calibration
DigiRead and ScanPlus as standard, other Windows™ software
Software:
packages available
Computer interface: EZ Read 2000 Mini USB.
UVM340 RS232 bidirectional and USB.
Power input: 90 to 250V AC, 50/60 Hz, 65 VA
Dimensions: 27cm x 43 cm x 24 cm (WxDxH)
Operating Temperature: +10 to + 35° C
Operating Humidity: 0 to 80 % ( Non condensing)

Humidity 5 to 95%, non-condensing (storage only)

Environmental conditions
Maximum altitude 2000m

EZ Read 2000 / UVM340 V4.0 7

3.0 Theory of Operation
3.1 Introduction
This chapter gives the description of the main components, the optical system layout and description.

3.2 Main components

The main components of the reader are the CPU board, the Interface board with the 14-bit A/D
converter and motor drivers, the plate transport mechanism, the light source, monochromator and the
power supply unit

EZ Read 2000 / UVM340 V4.0 8

3.3 Optical system

The optical system consists of the lamp, the condenser lenses, the beam splitter, wavelength reference
LED, the monochromator, the measurement and reference channel optic fibres and focusing lenses
below and above the microplate and silicon photodetectors. The light energy from the Lamp is
condensed by the Condensing lens and passes through the Beam Splitter, entrance slit and onto the inlet
mirror. The mirror directs the light to the Collimating mirror which directs the light onto the Grating
where the Wavelength is selected; it is then directed back onto the Collimating mirror then the Exit
mirror onto the Measurement Fibre Optic. From here it passes through a Focusing lens, an aperture,
through the microplate well, another focusing lens and onto the Measuring Photo diode. The energy
from the Reference diode is reflected by the Beam Splitter and follows the same course as the Lamp
energy until it reaches the Reference Fibre Optic where it travels to the Reference Photo Diode.

EZ Read 2000 / UVM340 V4.0 9

4.0 Electronics

4.1 Electronic Board Layout

RS232 / USB
Board

Power
Supply

Filtered Power
Input Socket
with ON / OFF
Lamp Control
switch and Fuse
Board
Holder

Interface
CPU Board Board

EZ Read 2000 / UVM340 V4.0 10

4.2 Power Supply

The power supply is a switched mode supply which supplies +5 and +24 volts
DC to the instrument with its own internal fuse.

Power Supply

Lamp Control
Board

4.3 Basic CPU Board Operation

The CPU Board is piggybacked on to the interface board and uses a 4MHz 8-bit High Performance PLCC
Microcontroller with a 512 byte on board EEPROM and 1024 bytes of on board RAM. This device and the
Flash memory device which contains the operating Firmware, control the operation of the reader.

EZ Read 2000 / UVM340 V4.0 11

4.4 Light Control Board Diagram
The Light Control Board is digitally controlled for greater lamp stability and control. The control is done
via the CPU Board piggy backed on the Interface Board. This digital control system prevents any drift
from the optimum operating range found on many currently used analogue light control systems. With
the digital control it is possible to detect lamp failure before the lamp reaches the end of its usable life.
The lamp voltage is controlled by a LT1074 switching regulator. The board also contains a DC-DC
converter to produce +/- 12DC which is used on the interface board and the preamp board.

4.5 Preamplifier Board

The Preamp Board uses two Silicone detectors (D3 and D4) with fast response times to measure the light
passing through the sample in the microplate and the reference channel. These analogue signals are
amplified and sent to the A-D Converter section of the Interface Board where they are converted to a
digital signal and used by the CPU Board to calculate the results.

4.6 Interface Board

The Interface Board is used to interface the peripheral devices to the CPU Board so that it can control
them. This board controls the Plate transport motors via IC’S 4 & 5 and the monochromator motor via
IC’S 6 & 7. The RS232 communications is done via IC2 and RS232 Interface Board mounted on the rear
panel.

EZ Read 2000 / UVM340 V4.0 12

5.0 Adjustments
5.1 Replacing the Lamp
1. Turn off the power and remove the power cable.
2. Remove the 6 screws which hold the top cover onto the base plate.
3. Lift the front of the cover carefully and unplug the cable connected to the power led.
Put the cover to one side.

Screws

4. Place the unit on its left hand side and remove the 5 Allen head screws. Unplug all the
cable attached to the top plate from the base plate noting their positions.

Lamp cover with

2 holding screws

EZ Read 2000 / UVM340 V4.0 13

 5. Place the unit on its front end and remove the 2 screws holding the lamp cover in
place and remove the cover.

Lamp Housing with Cover Removed

Lamp power
socket

Remove the lamp

by pushing it in
this direction

6. Remove the lamp power socket and push the lamp in the direction indicated. Insert
the lamp in the reverse procedure with the indentation in the lamp housing facing in
the direction indicated. The lamp must “click” into position.

7. Reassemble the unit and carry out the “Lamp Trim Procedure” below.

Warning: Do not touch the glass envelope of the lamp with your bare fingers, use lint free cloth
or optical lens paper.

Lamp
Indentation

EZ Read 2000 / UVM340 V4.0 14

5.2 Lamp Trim Procedure
Please follow these steps exactly as described here, do not swap procedures, and follow step by
step. The “Service tools” will be handed out during the training course. These two programs
should be loaded onto your PC and the reader connected via the USB or RS232 cable.

1. UVMTest.exe -> a tool that checks the basic functions and generates a text file
"UVM.txt".
2. Start the program and a text file will be generated. The file should look like the one below.
This can be emailed to: [email protected] for evaluation.
3. U340Srv.exe -> a useful service tool. It’s easy to use and you can do calibration of the
transport and light system.
4. Start U340Srv.exe
5. Choose "Service" and then "Lamp Trim"
A value of
“0“indicates the
Live lamp is off
value
shown
here

6. The window above appears. In the upper left corner you'll find the "Diode Values" and
next the "Lamp- Energy-Level". (The Lamp-Energy-Level must never be higher than 200! If
it is higher you have a problem with the "Light Control Unit!) The Diode value -> in the
picture above, “243” - is the “live” counts of the ADC (the lamp is off -> look at the “0” in
the "Lamp” row, so the counts are low!)

7. Remove the upper case of the instrument. Watch the diode value!
Due to stray light the value will change to 16383, which indicates an "Overflow" of the
ADC!

EZ Read 2000 / UVM340 V4.0 15

 8. In the centre of the UVM you'll find the optical sensor unit (including the A-D Converter).
From the front view you'll see at the very right hand side a blue coloured variable resistor;
this is the “Zero Adjust” potentiometer.
About 25mm from the left hand side you'll find a potentiometer. Here you'll have access to the
variable resistor and will be able to calibrate the “Bright” value

9. Before we start with the alignment you will have to cover the optical sensor unit with a
black cloth and make sure that you have access to the variable resistor (it is sealed).
Ensure the diode value is as low as possible! -> around 300

10. Select the first wavelength -> 800nm; push “Bright F3” and then “Lamp F6”. The reference
LED must be “OFF”. Watch the "Current Diode Value". It must not be higher than 15300 (±
100) -> valid range: 9500 – 15300. If the value is above 15300 you'll have an overflow.
(You will find the hole on the left hand side in which is the “Bright Value” adjustment.) In
that case turn the variable resistor counter-clockwise and watch the diode value. Turn the
resistor until the value is in range.

11. Now select the 340nm wavelength / filter. The reading must be more than 9500 without
changing any settings.

Tip: If you haven't any cloth available to cover the optical sensor unit, I would recommend the
following: Turn the variable resistor 5 turns counter-clockwise and replace the upper case. Have
a look at the diode values! Repeat this procedure until the values are lower than 15300.
******************************************************

EZ Read 2000 / UVM340 V4.0 16

 Typical UVM340 UVM.txt File
FirstStateRequest: OK
Initialize: OK
ReadSetup: OK
ScanWvlen: OK
ReadResultWvlen: OK
ScanRfled: OK
ReadResultRfled: OK
MoveHome: OK

Wavelength of Ref. LED (nm): 599.0

Position of Ref. LED (steps): 904
Intensity of Ref. LED (counts): 205
Tolerance for Ref. LED (percent): 50.0
Ref. LED Correction (counts): -3
Wavelength 800 - 340 Ref LED
nm counts nm counts nm counts nm counts nm counts nm counts nm counts nm counts nm counts nm counts nm counts
800 14619 749 14892 698 15168 647 15290 596 15311 545 15249 494 15175 443 14513 392 13534 341 11600 619 165
799 14624 748 14898 697 15167 646 15291 595 15311 544 15245 493 15176 442 14500 391 13512 340 11494 618 167
798 14629 747 14897 696 15168 645 15292 594 15315 543 15246 492 15182 441 14483 390 13495 617 170
797 14633 746 14898 695 15176 644 15292 593 15311 542 15244 491 15189 440 14469 389 13475 616 170
796 14637 745 14898 694 15178 643 15293 592 15309 541 15239 490 15192 439 14460 388 13453 615 170
795 14640 744 14901 693 15185 642 15293 591 15307 540 15239 489 15194 438 14444 387 13430 614 177
794 14649 743 14904 692 15190 641 15297 590 15308 539 15236 488 15203 437 14429 386 13403 613 180
793 14657 742 14906 691 15191 640 15294 589 15304 538 15235 487 15208 436 14422 385 13369 612 185
792 14660 741 14912 690 15197 639 15298 588 15304 537 15233 486 15210 435 14396 384 13334 611 193
791 14666 740 14911 689 15196 638 15296 587 15307 536 15229 485 15218 434 14385 383 13306 610 208
790 14674 739 14917 688 15196 637 15297 586 15306 535 15228 484 15223 433 14375 382 13268 609 223
789 14675 738 14924 687 15200 636 15296 585 15301 534 15226 483 15235 432 14358 381 13230 608 245
788 14678 737 14925 686 15203 635 15299 584 15304 533 15224 482 15242 431 14340 380 13197 607 266
787 14685 736 14925 685 15208 634 15299 583 15302 532 15222 481 15253 430 14322 379 13166 606 294
786 14683 735 14934 684 15206 633 15296 582 15296 531 15218 480 15264 429 14305 378 13121 605 317
785 14686 734 14940 683 15210 632 15297 581 15295 530 15217 479 15274 428 14293 377 13087 604 337
784 14689 733 14946 682 15215 631 15298 580 15294 529 15213 478 15286 427 14270 376 13049 603 350
783 14691 732 14954 681 15214 630 15298 579 15294 528 15209 477 15303 426 14256 375 13014 602 358
782 14686 731 14960 680 15218 629 15295 578 15292 527 15209 476 15317 425 14235 374 12979 601 356
781 14689 730 14969 679 15217 628 15297 577 15292 526 15199 475 15338 424 14219 373 12948 600 348
780 14681 729 14976 678 15222 627 15291 576 15294 525 15196 474 15351 423 14204 372 12909 599 341
779 14685 728 14982 677 15219 626 15292 575 15284 524 15199 473 15369 422 14181 371 12879 598 331
778 14678 727 14992 676 15221 625 15293 574 15287 523 15193 472 15387 421 14166 370 12851 597 321
777 14674 726 15002 675 15220 624 15292 573 15285 522 15193 471 15406 420 14149 369 12816 596 311
776 14655 725 15011 674 15227 623 15292 572 15281 521 15188 470 15416 419 14132 368 12791 595 299
775 14635 724 15024 673 15225 622 15290 571 15281 520 15187 469 15427 418 14111 367 12763 594 295
774 14617 723 15033 672 15231 621 15295 570 15281 519 15187 468 15430 417 14091 366 12732 593 286
773 14612 722 15038 671 15237 620 15295 569 15278 518 15183 467 15434 416 14080 365 12701 592 279
772 14616 721 15049 670 15235 619 15299 568 15277 517 15183 466 15427 415 14057 364 12669 591 271
771 14629 720 15056 669 15239 618 15305 567 15276 516 15179 465 15409 414 14039 363 12643 590 262
770 14657 719 15064 668 15244 617 15306 566 15274 515 15180 464 15383 413 14024 362 12619 589 254
769 14685 718 15069 667 15244 616 15305 565 15269 514 15181 463 15349 412 14006 361 12589 588 245
768 14712 717 15079 666 15247 615 15304 564 15273 513 15176 462 15301 411 13983 360 12561 587 236
767 14740 716 15082 665 15249 614 15309 563 15271 512 15177 461 15256 410 13963 359 12527 586 227
766 14757 715 15086 664 15254 613 15307 562 15269 511 15179 460 15185 409 13940 358 12498 585 220
765 14778 714 15091 663 15257 612 15310 561 15272 510 15177 459 15124 408 13922 357 12461 584 213
764 14789 713 15096 662 15258 611 15315 560 15270 509 15175 458 15057 407 13892 356 12422 583 206
763 14806 712 15102 661 15263 610 15312 559 15270 508 15175 457 14968 406 13860 355 12386 582 197
762 14813 711 15113 660 15264 609 15306 558 15267 507 15175 456 14891 405 13837 354 12346 581 193
761 14828 710 15113 659 15267 608 15313 557 15266 506 15172 455 14819 404 13812 353 12298 580 186
760 14835 709 15119 658 15273 607 15311 556 15263 505 15172 454 14757 403 13780 352 12254 579 180
759 14846 708 15119 657 15270 606 15313 555 15264 504 15171 453 14693 402 13757 351 12207
758 14854 707 15127 656 15274 605 15314 554 15264 503 15168 452 14641 401 13726 350 12157
757 14861 706 15130 655 15273 604 15317 553 15257 502 15167 451 14597 400 13706 349 12108
756 14862 705 15137 654 15279 603 15315 552 15258 501 15170 450 14564 399 13679 348 12053
755 14872 704 15144 653 15279 602 15314 551 15255 500 15170 449 14550 398 13661 347 11993
754 14876 703 15151 652 15286 601 15319 550 15254 499 15170 448 14541 397 13634 346 11924
753 14880 702 15151 651 15286 600 15313 549 15255 498 15168 447 14536 396 13614 345 11861
752 14884 701 15159 650 15288 599 15314 548 15254 497 15172 446 14541 395 13594 344 11824
751 14887 700 15158 649 15287 598 15314 547 15253 496 15174 445 14536 394 13573 343 11762
750 14890 699 15168 648 15290 597 15311 546 15249 495 15175 444 14525 393 13551 342 11681

EZ Read 2000 / UVM340 V4.0 17

5.3 Lamp Voltage Check

Open the UVM340 Service Program and click on: Service, Lamp Trim, F6 (switch on lamp) and
select the 340nm wavelength. Using a test meter, connect the probes to the lamp power socket.
The reading must be below 11.8vDC.

5.4 Wavelength Calibration Check

Open the UVM 340 Service Program and click on: Service, Wavelength Scan and Scan. Check that
the Start Wavelength is set at 340.0 and the End Wavelength is set at 800.0 and the lamp is ON.
Then click OK. The resulting Wavelength Scan should be between 10,000 and 15,300. Place the
cursor over the highest and lowest peak to check if the values are within range.
The scan should look similar to the one below.

EZ Read 2000 / UVM340 V4.0 18

5.5 Wavelength Calibration setting
This calibration requires the Holmium Oxide Calibration Plate # 80-4000-05.

1. From the main screen select “Service” then “Holmium Filter” and the screen below will
be displayed.

2. Enter the calibration wavelength 536.5nm.

3. Click on “F2: Calibrate”, this will initialize the wavelength calibration procedure. When
requested insert the “Calibration Plate”.
4. Once this is completed click on “F3: Initialize”, this will calibrate the “Reference LED”.
Select “Service” then “Well Scan” and then “Scan”.
5. When the screen below is displayed using the cursor adjust the “UP / DOWN” arrows to
change the Value to “D”, then with the “LEFT / RIGHT” arrows change the value to “3”.
Click on “OK” to start the scan.

Use these Use these

arrow keys to arrow keys to
change the change the
value to “D” value to “3“

EZ Read 2000 / UVM340 V4.0 19

 6. The completed scan should look like the one below. Using the cursor; read the value of
each peak. The ADC value will appear in the left hand column while the wavelength will
appear in the bottom row. The minimum ADC value obtained at each peak will be the
apex of that peak.

ADC values

638nm

419nm
537nm

361nm

447nm

Wavelength

5.6 Monochromator replacement.

This article applies to the following fault(s):
 The monochromator has already been identified as the faulty part
 The motor which drives the monochromator gear wheel jams during instrument set-up
 The monochromator does not seem to show any sign of mechanical movement
Removal & Disassembly
1. Remove instrument cover (5 bolts)
2. Turn instrument upside down and remove 3 of the 5 bolts; holding the upper assembly
to the lower.
3. Return instrument to normal orientation with a corner hanging over your work surface
so you can remove 1 of the 2 remaining bolts. Turn instrument and remove final bolt.
4. Ensure that all wiring is unplugged that attaches the lower assembly to the upper,
then separate the two.
5. Place lower assembly to one side and work on the upper assembly
6. Place the upper assembly on its side ensure you have something soft for the sliding
well tray mechanism to be placed upon. This will ensure it is not damaged or offset.
7. Loosen the two screws holding the motor in place (remove if necessary) and remove
the belt.
8. Undo the retaining bolt which holds the fibre optic cable inside the monochromator
and gently withdraw the fibre optic cable, detach the other two ends of the cable and
withdraw the entire fibre optic loom.

EZ Read 2000 / UVM340 V4.0 20

IMPORTANT: Do not touch the ends of the fibre optic cable; this is likely to cause irreversible
damage!

9. Undo the two bolts on the opposite side of the assembly that the monochromator is
located and withdraw the unit and the spacer.
10. Undo the retaining bolts on the gear wheel attached to the monochromator and
remove it.
11. With a blade or other suitable instrument, gently slide out the mask from the
monochromator housing.
Installing a new Monochromator
1. Attach the gear wheel to the new monochromator.
2. Slide the mask into the large hole in the side of the new monochromator. Fig.1

Fig.1 Fig.2
3. Ensure that you have placed the calibration certificate for the new monochromator in the
instrument file.
4. Insert monochromator back into the chassis and replace screws loosely.
5. Insert the alignment tool into the fibre optic chassis housing and up into the
monochromator. (See Fig.2)
6. Tighten the screws holding the monochromator to the chassis.
7. Remove the alignment tool.
8. Re-insert the fibre optic cable into the monochromator; push it fully up, then withdraw it
0.5 – 1mm before tightening the retaining bolt.
9. Re-insert the other two ends of the fibre optic loom into their sockets ensuring that they
do not cross over each other, Fig.3.
10. Re-attach the belt onto the gear wheel on the monochromator and the gear wheel on the
motor, Fig.4.

Fig.3 Fig.4

EZ Read 2000 / UVM340 V4.0 21

 Alignment Tool
11. Ensure the motor is aligned parallel with the instrument chassis and tighten bolts holding
it to the chassis.
12. Ensure that the belt is slacked to approximately 5mm of movement when pinching the
belt between both gear wheels.
12(a). Move the position of the motor laterally by loosening the bolts if the belt is not
tensioned correctly, then re-tighten.
13. Place the bottom half of the instrument assembly in your working area and introduce the
upper half of the assembly on top of it.
14. Completion of instrument assembly is reversal of steps 1 – 4 from the removal process.
15. You will now need to perform a wavelength calibration.

5.7 Transport Calibration

This calibrates the Plate transport (X and Y) and the Monochromator transport. Open the UVM
340 Service Program and click on: Service, Transport and F6, calibrate Y-Transport first then click
on F5 and calibrate X-Transport. Then click on F7 and calibrate the Monochromator transport.

EZ Read 2000 / UVM340 V4.0 22

5.8 Positioning Setup
If the instrument positioning fails within the WinQC test or you suspect positioning to cause you a fault,
follow this guide.

You will require the use of our internal software called UVMProd.exe; this software is
recommended for use by trained Asys service staff only.

Tools required:
 UVMProd.exe Setup Software
 Asys QC Test Plate
 2mm Hex Driver
 2.5mm Hex Driver (if parallel adjustment required)
 3mm Hex Driver
 Small piece of card
 Small piece of tracing (or similar) paper

1. Place the instrument sideways so that the front door is facing your left.
2. Undo the six 2mm bolts holding the top cover to the instrument
3. Remove the cover from the rear of the instrument as the LED wire is attached to the front
internally.
4. Place the cover on its front, to the left of the main assembly. Take care not to stretch the
LED wire.
5. Undo and remove the 3mm bolt on the upper lens block assembly.
6. Whilst leaving the diode board connected, carefully remove the upper lens block
assembly and place behind the instrument facing upwards as shown.

EZ Read 2000 / UVM340 V4.0 23

7. Switch on the instrument and connect to your PC
8. Place the Asys QC test plate into the plate carrier.
9. From UVMProd.exe and select Service > Transport, you will be presented with this
screen:

10. Select ‘HOME’ from the X-Transp. Window first, then select ‘HOME’ from the Y-Transp.
This ensures that the plate carrier moves to the home positions.
11. Now enter these default positions (1080/868); always start with X before Y and press the
return key on your keyboard after inputting the position:

This will send the plate carrier to where it believes hole A1 of the Asys Test plate is
located.

EZ Read 2000 / UVM340 V4.0 24

12. To produce some light from the measurement & reference channels, we need to set the
lamp and monochromator. First, cover the reference lens with some card as shown:

13. From UVMProd.exe, select Service > Lamp Trim, then select F6 button to switch on the
lamp. ‘Lamp is ON’ should appear.

EZ Read 2000 / UVM340 V4.0 25

14. Manually turn the gear wheel which drives the monochromator until the light from the
lenses is bright as shown:

15. Place some tracing paper (or similar thin paper) over hole A1 of the plate to see where
the light beam is in relation to the hole.

EZ Read 2000 / UVM340 V4.0 26

16. If the plate is not aligned, use these diagrams to work out how the parameters need to be
changed:

Increase Y values
to move plate this
way Y axis

Decrease Y values
to move plate this
way

X axis

Increase X values Decrease X values

to move plate this to move plate this
way way

In this example:-
Decrease X
Decrease Y

In this example:-
Increase X
Decrease Y

Note: the difference between the edge and the centre

of the lens is equivalent to approx. 10 units

EZ Read 2000 / UVM340 V4.0 27

17. Repeat steps 10 – 15 whilst changing the parameters in step 11 until the light beam is
perfectly central to the hole.
18. When you have found the optimum position, select Service > Transport
19. Re-type the correct parameters into the X and Y ‘Move to’ text box, ensuring you press
the return key after each input.

20. Now select Transport > Define X/Y as > A/1 of Plate 8*12. If you have done this
correctly, the screen above in step 19 should ‘blink/flash’ once.

21. To verify the parameters have been stored, click Service > List Setup. The bottom of the
setup page shows the stored plate parameters.

EZ Read 2000 / UVM340 V4.0 28

Parallel Verification
It is important to ensure that the plate carrier is set up on an exact right angle. To do this follow
these instructions after performing steps 1 – 21 above.

a) Now that you have obtained the correct position of A/1 of the Asys Test plate, we need to
check the alignment of the plate carrier.
b) From UVMProd.exe, select Service > Transport
c) Select ‘HOME’ from the X-Transp. Window first, then select ‘HOME’ from the Y-Transp.
This ensures that the plate carrier moves to the home positions.

d) Calculate the position of A/12 by using the normal X-position of A/1 – 990. For example,
normal X = 1080, 1080 – 990 = 90. 90 = A/12
e) Type the new A/12 position into the X-Transp. text box and press the return key. Now
enter the normal Y-Transp. Figure into the Y.Transp. text box and press the return key.

EZ Read 2000 / UVM340 V4.0 29

 f) Repeat steps 12 – 14 so that light goes through the test plate.
g) Place some tracing paper (or similar thin paper) over hole A/12 of the plate to see where
the light beam is in relation to the hole. The light beam must be perfect central.
h) If it is not, you should make fine adjustments to the plate carrier by loosening the three
M2.5 bolts holding the plate carrier to the plate carrier arm.
i) Re-check the plate alignment of both A1 and A12 after any adjustment of the plate carrier
by following all steps above (1 – 21 & a – i).

 Replace upper lens block and fix with 3mm bolt removed in step 5
 Re-fit instrument cover and fix with six 2mm bolts removed in step 2

5.9 ADC Adjustment

Please request and obtain the U340Srvc.exe application

1. Switch the UVM340 instrument on.

2. Open the U340Srvc.exe software and connect to the instrument using either the USB or
the RS232 lead.
3. Select the correct COM port then click OK.

EZ Read 2000 / UVM340 V4.0 30

Darkness Value
1. Make sure you have the top cover on the instrument or a black cloth over it to block out
the light.
2. Select “Service” and then “Lamp Trim”

3. The diode reading should be approx. 200 counts; it is likely to vary by approx 10 counts as
it is very sensitive. If the diode reading is not correct, remove the instrument top cover
and adjust the potentiometer on the edge of the AD converter board:

Please note this potentiometer is very sensitive, only make small adjustments. Replace
the top cover, and then re-check the diode reading with the UVMSrvc.exe application
until correct.

EZ Read 2000 / UVM340 V4.0 31

General Diode Adjustment
1. From UVMSrvc.exe application, select Service > Wavelength Scan
2. Select Scan > Scan and ensure all options are as displayed:

3. Ensure no plate exists on the plate carrier then press OK to begin a wavelength scan. The
diode counts read for all wavelengths should be between 10,000 – 15,500, for example:

4. If at any point the counts read out of the range specified in step 3, adjustment is required
via the potentiometer located in the circular cut-out on the AD converter board shield:

EZ Read 2000 / UVM340 V4.0 32

 5. Remove the top cover to make small adjustments, always replace the top cover before
carrying out a new wavelength scan. Repeat until all results are within tolerance (10,000
– 15,500)

After major adjustment is made to the optical system, it is necessary to re-

calibrate the optical system with the use of a holmium calibration filter.

EZ Read 2000 / UVM340 V4.0 33

5.10 Top cover adjustment

If the top cover of the UVM340 has become loose or incorrectly seated, this document
provides an engineer with instructions for correct fitment.

1. Loosen the four 2.5mm hex bolts on the sides of the top cover (2 on each side)

2. Lift the front of the top cover until the front end of the instrument begins to lift
away from the workspace below.

3. Whilst holding the top cover, re-tighten the front bolts, this ensures the top cover is
at its highest point possible.
4. Return the instrument to normal placement on your work surface and tighten the
rear bolts.

EZ Read 2000 / UVM340 V4.0 34

6.0 Performance Verification
The UVM- 340 reader performs several checks automatically before every measurement. These
checks include the measurement of the light intensity, the stability of the readings and the
correct movement of the X/Y drive and monochromator. In addition the correct wavelength
setting of the monochromator is checked every time the instrument is switched on. If an error is
detected during one of these checks a corresponding error message is displayed and the reading
is stopped.

6.1 QC Plate:

To check the performance of the Instrument, we recommend to the ASYS Hitech QC plate
(SG010160) with the WinQC software. The QC plate checks the mechanical alignment of both the
plate transport and the optical path and tests the accuracy and precision.
The QC-plate consists of a precise milled aluminium body in the dimensions of a standard
microplate and three neutral density glass filters. In contrast to other neutral density filters the
sensitive coated area is protected by another glass layer, avoiding the known problems with
scratches and degradation caused by frequent cleaning. The neutral density filters cover all of the
eight measurement channels.
For instructions on how to use the QC-plate and WinQC - software see the manual which comes
with the QC-plate.

6.2 Orange Test Plate:

An alternative to the QC-plate is the so-called ‘Orange Test plate’ (S5450174). The results
obtained with this plate are not as accurate as with the QC-plate but usually sufficient for a basic
check.
The ‘Orange Test Plate’ is a simple tool to check the correct wavelength and function of an
interference filter. This is performed by comparing the enclosed reference OD and scan data
with data obtained by a measurement using the wavelength in question.
Furthermore, the results of the measurement can demonstrate the correct function of the
reader.

Note: The correlation of the reference data with the measured data does not prove that the
wavelength is correct when using this plate.

EZ Read 2000 / UVM340 V4.0 35

Operation of the Orange Test Plate
1. Make sure that the surface of the plate is free of dirt and fingerprints.
2. Perform single wavelength measurements with the filter in question and print the results.
3. Compare the results with the reference data. The results should be within +/- 20% and +/- 0.005
OD of the reference data for the wavelength used. If the result is off, most likely the filter is
defective or has a wrong wavelength.
4. Check for similar readings in the 8 rows. There should be no more than +/- 6 % and +/- 0.005
OD difference between the readings of different rows in the same column.

NOTE: For a complete performance check of the Instrument, use the QC plate with the
WinQC software.

7.0 Error Codes

Value Description
1 General system error
2 Communication error, communication interrupted, transport error
3 Specified filter not defined
4 Zero reference too low (dark reading is too low, detector fault)
5 Zero reference too high
6 measurement values in overflow during self-check
7 measurement values zero during self check
8 stability of measurement values out of range during self check
9 measurement values too low
10 Time-out
11 Transport home position error ( too soon)
13 Transport home position error ( too late)
14 Transport error (time-out second sensor)
15 Transport error (invalid sensor status)

EZ Read 2000 / UVM340 V4.0 36

8.0 Troubleshooting
8.1 Basic Troubleshooting
PROBLEM POSSIBLE CAUSE
Check plugged in and switched on.
Check power on light is ON.
Check Fuse. This can be replaced, but only by the same
Reader fails to start
type and rating. Note: Before replacing the fuse,
up
disconnect the power cord.
Check for jammed plate transport or failure.
Check lamp comes on.
Check to see if samples are cloudy or mixed properly. If
the sample is not mixed properly, sediments can settle at
the bottom of the well which can lead to measurement
discrepancies.
Check for bubbles in solution.
Check plate orientation.
Check for poor, scratched plates.
Check if the correct plate format is used (96 well plate,
flat, round and v-bottomed well formats with standard
Readings are higher or
ANSI SBS footprint).
lower than expected
Check for condensation on plate base.
If filters have been changed check that the filter positions
have been entered and calibrated correctly
Check assay preparation steps have been followed
correctly.
Check correct sample volume has been dispensed. This is
important as path length (which is used to calculate
sample concentration using Beer’s Law) is not
standardised and is dependent on sample volume.
Therefore the volume must be equal throughout the plate.
Check plate types used are suitable.
Check that plate transport passes start up checks. This
refers to when the instrument finds the home position of
the plate immediately after being switched on. If the plate
reader has a display the status of the check is shown.
Readings have poor Check for any bubbles or wells where there is foam (likely
repeatability and are to arise from pipetting or shaking).
random or noisy Use a background wavelength correction. For example
measure the sample absorbance at 405nm and
wavelength reference at 620nm.
Check sample dilutions are such as to produce a response
within the range 0.1 to 2.5 A. This is the recommended
linear range of the plate readers.

EZ Read 2000 / UVM340 V4.0 37

 Check assay preparation steps have been followed
correctly.
Check correct sample volume has been dispensed, as
mentioned above.
Use a polystyrene plate with no scratches or evaporation
which may affect absorbance readings.
Check that pipettes (single and/or multi-channel) dispense
the correct set volume.
Recommended good Check that the correct tips are used for the appropriate
laboratory practice pipettes.
Check plate for any bubbles or foam as mentioned
previously.
Check readings with a QC plate (refer to user manual for
specified plate) refer to section 1.4.

8.2 Advanced Troubleshooting

Fault Cure
 If the reading is not much more than the dark reading (E.g
<2000), it can be because the monochromator is completely
out of adjustment and so is not actually at 340nm. Switch off
instrument, calibrate monochromator, reset instrument again.
 Did you restore the DATA file? (File > Restore > Data). If you
need to carry this out, then you will need to follow all steps
after page 7 of the set-up guide (starting at Calibrate
Transport)
 If the reading is between 2000 and 8500, then a likely problem
Instrument reads less is that the condenser lens has moved and the lamp image is no
than 9500 on 340nm longer focussed on the input slit of the monochromator.
filter.  If the reading is between 8500 and 10000, it may just be a
matter of cleanliness of the lenses or the photodiodes as
fingerprints selectively soak up UV and 340nm is at the UV end
of the spectrum. Try cleaning the photodiode windows first,
followed by the upper lenses and then the ends of the fibres.
 Try swapping the fibres over
 Try turning the main fibre optic end about 45 degrees, re-
tighten screw and re-test.
 Change fibre optics
 Exchange the light regulator board
 Switch off instrument, calibrate monochromator, reset
instrument again.
Instruments reads  Ensure resistor R7 on the pre-amp PCB is 3.3K not 8.2K
maximum value (16,383) (Original type). If it is a 3K resistor and the fault still occurs try
on 800nm/all filters. an even lower resistor, e.g. 2.2k
 Adjust pot under pre-amp cover (the pot with circular cut-out
in cover above it)
EZ Read 2000 / UVM340 V4.0 38
  Ensure LED cable is plugged into interface board
 Plug LED cable into power supply and ensure the LED is
Reference LED counts working correctly
less than 70  Clean the tip of the LED
 Try new LED
 Try new monochromator (Last resort!)
‘Sensor found too soon’  Re-check load position of X-transport and re-calibrate
error transport if adjustment was necessary.
Launch U340prod.exe program:
If 1st row fails click on: Reader > Setup > Measurement unit >
Instrument fails Position corrections for the measurement scan in Y. Adjust
positioning proof settings minus 1 or 2 and retest.
If 2nd row fails click on: Reader > Setup > Plate definitions.
Adjust Y A/1 value 1 or 2 steps and retest.
Re-check your lamp trim values, re-adjust if necessary ensuring
Instrument Error 4 when you run a holmium filter scan, ref LED calibration and wavelength
trying to carry out a scan after adjustment. Switch off unit, wait for internal clunk and
measurement in Digiread turn back on. Re-check lamp trim, if not OK, re-adjust as above, if
OK, retry Digiread measurement.
Holmium filter calibration Re-check the load position setting, it is set too high. Bring it down
stops before but ensure you can still easily insert a test plate without the spring
measurement takes catch getting in the way.
place, further error
information says 2nd
sensor reach too soon.
Holmium filter calibration Adjust pot under black shield on AD board to bring all values down
stops before slightly. You will find if you can do a wavelength scan that the
measurement takes peak will be above 16,300 at some point.
place, error says
something to do with
holmium peak too high?
Fluid plate reads high on  Back-Up data using File > BackUp > Data from UVMProd.
channel A only. Replace CPU board with new and re-program firmware,
Transport motors seem then restore your back-up you made.
to be jammed.  Check links have been soldered on interface board (J1 & J3)

Check position of measurement lens, it must be perfectly central

Fluid plate reads too low
for optimum result. If it is out of place, ensure you re-position the
on 620nm filter only
upper measurement lens in the AD board assembly if necessary.
 Ensure belt tension is not too loose, reasonably tight.
Y transport too late error  Ensure wiring is tidy
(seems to show during  Calibrate Y transport with holmium filter in place, extra
holmium scan) weight of plate may be causing fraction of stress to Y
transport motor
Transport seems jammed  Ensure CPU has had firmware loaded onto it

EZ Read 2000 / UVM340 V4.0 39

  Check links have been soldered on interface board (J1 & J3)
Communication error  Try again, may take a few attempts
when trying to program  Check/change mainboard
 Check photo-diodes clean.
Empty plate test not in
 Change AD board
spec
 Lamp drift, change lamp
X/Y plate carrier tries to  Check motor wires have been reversed
go further back than the
sensor
Wavelength  Swap reference channel with measurement channel of fibre
measurement shows very optics
high reading at 340nm  Change fibre optics
(reversed graph)
 Listen to monochromator when calibrating, if the sound is
Distortion in wavelength
pulsing slightly, increase the power to the monochromator
scan, line plotted shows
motor with the small blue pot located on the mainboard.
pulses of diffraction. Line
Do this until the sound is smooth, then re-calibrate the
is not smooth as usual
monochromator and re-scan.
 Re-setup instrument with holmium filter plate from
Instrument Error 11 in
UVMprod.exe
Digiread
Measure a 96 hole plate, if every other row is good (under 0.004)
then follow these instructions to remedy:
1. Remove cover and AD board, connect lamp to lamp PSU
2. Turn monochromator motor gear until light from
measurement lens is bright green
3. Fit 96 hole plate or Asys QC plate and set transport
positions (not mono) from software to ‘home’
4. Re-set plate parameters if necessary to ensure A1 position
is central to light beam
5. Set X parameter to -990 of value (E.g. if value is 1086, send
plate carrier to 96), this will send it to position A12
6. Be careful not to move plate carrier, check that light beam
Positioning fails (Columns is still central to hole, if it is not, make adjustments to
1 & 12 not under 0.004) screws holding plate carrier to transport mechanism.
Repeat above steps until both holes are central to light
beam. Parallel positioning is now perfect.
7. Re-attach AD board and cover and repeat a measurement
with a 96 hole plate. If every other reading is still bad,
make adjustments to Y Position Correction value from
software until all reading are similar (If they are good
(under 0.004) then finish, if not continue to next step.
8. Repeat plate positioning setup with 96 hole plate or Asys
QC plate as per instructions in the build file OR you can try
adjusting the plate positioning parameters directly from the
software and checking with 96 hole plate measurement
until correct.
EZ Read 2000 / UVM340 V4.0 40
 Place your finger on the monochromator gear wheel and switch on
the instrument. If the gear wheel buzzes after the transport has
Monochromator motor
initialised you will feel the vibration through your finger. This
does not move when
shows that the monochromator is receiving power just not
switching on instrument
enough, increase the power to the monochromator by turning the
pot on the interface mainboard anti-clockwise.
 Check lamp alignment with monochromator slit
 Move condenser lens to try to make image a little more
Wavelength appears blurred
jaggered and may drop  There may be too much current to the monochromator
on right hand side causing vibration when taking a wavelength scan. Reduce
the amount of current to the monochromator by turning
the pot on the interface mainboard clock-wise.

9.0 Advanced System Errors

The advanced error codes are transferred as an answer string to command Ex. The error values
listed here are hexadecimal and are also sent to the host in this format.
The following advanced system error codes are currently defined:

9.1 In relation to CSYS_RUNTIME

Constant Value Description

ECD_NOSUPPORT 0x0110 The requested functional feature is not supported
ECD_WRFAILED 0x0111 Write access failed
ECD_WRSIZE 0x0112 Invalid number of bytes to write
ECD_MEMOVERLAP 0x0113 The specified memory area overlaps
ECD_NULLPTR 0x0114 Invalid pointer in the memory area
ECD_MES_NOTPREP 0x0116 Measurement not prepared
ECD_MES_INVMPOS 0x0117 Invalid measuring position
ECD_MES_INVMTYP 0x0118 Invalid measuring type

EZ Read 2000 / UVM340 V4.0 41

9.2 In relation to CSYS_TRPERR
Constants Value Description
ECD_MX_INVSENS 0x0210 Invalid sensor status on the X-axis (reason for HOME or LIGHT
could not be determined)
ECD_MX_INVHSENS 0x0211 Invalid status of HOME sensor on the X-axis
ECD_MX_INVESENS 0x0212 Invalid status of LIGHT sensor on the X-axis
ECD_MX_TIMEOUT 0x0213 Sensor position was not reached within the given time
ECD_MY_INVSENS 0x0220 Invalid sensor status on the Y-axis (reason for HOME or LOAD
could not be determined)
ECD_MY_INVHSENS 0x0221 Invalid status of the HOME sensor on the Y-axis
ECD_MY_INVESENS 0x0222 Invalid status of the LOAD sensor on the Y-axis
ECD_MY_TIMEOUT 0x0223 Sensor position was not reached within the given time
ECD_TRPX_NOADJP 0x0230 Invalid alignment position index
ECD_TRPX_INVCALIB 0x0231 The X-transport was not calibrated or the calibrated data is
invalid.
ECD_TRPX_INVACTP 0x0232 The current position of the X-transport is invalid, therefore
destination positioning is currently not possible (prior
alignment must be carried out)
ECD_TRPX_HOMESOON 0x0233 The HOME sensor of the X-Transport was reached too soon
ECD_TRPX_HOMELATE 0x0234 The HOME sensor of the X-Transport was reached too late
ECD_TRPX_END1SOON 0x0235 The edge of the X-transport LIGHT sensor, which is close to the
HOME sensor, was reached too soon
ECD_TRPX_END1LATE 0x0236 The edge of the X-transport LIGHT sensor, which is close to the
HOME sensor, was reached too late
ECD_TRPX_END2SOON 0x0237 The edge of the X-transport LIGHT sensor, which is close to the
loading position, was reached too soon
ECD_TRPX_END2LATE 0x0238 The edge of the X-transport LIGHT sensor, which is nearer to
the loading position, was reached too late
ECD_TRPY_NOADJP 0x0240 Invalid alignment position index
ECD_TRPY_INVCALIB 0x0241 The Y-transport was not calibrated or the calibrated data is
invalid.
ECD_TRPY_INVACTP 0x0242 The current position of the Y-transport is invalid; therefore
destination positioning is currently not possible (prior
alignment must be carried out).
ECD_TRPY_HOMESOON 0x0243 The HOME sensor of the Y-transport was reached too soon
ECD_TRPY_HOMELATE 0x0244 The HOME sensor of the Y-transport was reached too late
ECD_TRPY_ENDSOON 0x0245 The LOAD sensor of the Y-transport was reached too soon
ECD_TRPY_ENDLATE 0x0246 The LOAD sensor of the Y-transport was reached too late

EZ Read 2000 / UVM340 V4.0 42

9.3 In relation to CSYS_FLTERR
Constants Value Description
ECD_MF_INVSENS 0x0310 Invalid sensor status (either HOME or END)
ECD_MF_INVHSENS 0x0311 Invalid status of HOME sensor
ECD_MF_INVESENS 0x0312 Invalid status of the END sensor
ECD_MF_TIMEOUT 0x0313 Sensor position not reached within the prescribed time
ECD_TRPF_NOADJP 0x0320 Invalid alignment position index.
ECD_TRPF_INVCALIB 0x0321 The transport of the monochromator was not calibrated, or the
calibrated data is invalid.
ECD_TRPF_INVACTP 0x0322 The current position of the monochromator is invalid; therefore
destination positioning is currently not possible (prior alignment
must be carried out).
ECD_TRPF_HOMESOON 0x0323 The HOME sensor of the monochromator was reached too soon
ECD_TRPF_HOMELATE 0x0324 The HOME sensor of the monochromator was reached too late
ECD_TRPF_ENDSOON 0x0325 The END sensor of the monochromator was reached too soon
ECD_TRPF_ENDLATE 0x0326 The END sensor of the monochromator was reached too late

9.4 In relation to CSYS_ADCLOW

Constants Value Description
ECD_CAL_STABHIGH 0x0410 A stability value is at the maximum level of the AD converter
ECD_CAL_DARKUFLW 0x0411 The dark value is at the minimum level of the AD converter
ECD_CAL_HOLMLOW 0x0412 During a search of the holmium filter a measuring value was at
the minimum level of the AD converter.
ECD_CAL_RLEDLOW 0x0413 During a search of the Reference LED peaks a measuring value
was at the minimum level of the AD converter.

9.5 In relation to CSYS_ADCHIGH

Constants Value Description
ECD_CAL_STABLOW 0x0510 A stability value is at the minimum level of the AD converter
ECD_CAL_HELLOFLW 0x0511 Hell value is at the maximum level of the AD converter
ECD_CAL_HOLMHIGH 0x0512 During a search of the holmium filter a measuring value was at
the maximum level of the AD converter
ECD_CAL_RLEDHIGH 0x0513 During a search of the reference LED peaks a measuring value
was at the maximum level of the AD converter

9.6 In relation to CSYS_MVOOMTOL

Constants Value Description
ECD_CAL_INVHELL 0x0610 The light value is smaller than the minimum permitted
ECD_CAL_RLEDIVAL 0x0611 The intensity of the reference LED varies by more than the
permitted tolerance of the calibrated value
ECD_CAL_RLEDIPOS 0x0612 The position of the reference LED varies by more than the
permitted tolerance of the calibrated position (not used!)

9.7 In relation to CSYS_MVOOSTAB

Constants Value Description
ECD_CAL_INVSTAB 0x0710 One of the stability values is outside the permitted tolerance of the
stability median

EZ Read 2000 / UVM340 V4.0 43

9.8 In relation to CSYS_CHTLOW
Constants Value Description
ECD_CAL_INVDARK 0x0810 The dark value is greater or the same as the light value

9.9 In relation to CSYS_WVLCALIB

Constants Value Description
ECD_WVLC_HOMEWVL 0x0b10 The wavelength of the monochromator HOME sensor has not
yet been set.
ECD_WVLC_HOLMWVL 0x0b11 The wavelength of the HOLMIUM filter is invalid
ECD_WVLC_HOLMPOS 0x0b12 The position of the HOLMIUM filter is invalid
ECD_WVLC_RLEDWVL 0x0b13 The wavelength of the reference LED is invalid
ECD_WVLC_RLEDPOS 0x0b14 The position of the reference LED is invalid
ECD_WVLC_VLOW 0x0b15
ECD_WVLC_VHIGH 0x0b16 The peak of the holmium filter is greater than the pre-set
maximum value
ECD_WVLC_HLMMIN 0x0b17 The permitted maximal value of the holmium peak is not
defined
ECD_WVLC_RLDTOL 0x0b18 Tolerance value for testing the recalibration of the reference
LED is not defined
ECD_WVLC_NOINIT 0x0b19 The reference LED has not been recalibrated

9.10 In relation to CSYS_VALOORNG

Constants Value Description
ECD_VOOR_MOTIDX 0x0c10 Invalid motor index
ECD_VOOR_MOTXSPD 0x0c11 Invalid speed parameter for motor X
ECD_VOOR_MOTYSPD 0x0c12 Invalid speed parameter for motor Y
ECD_VOOR_MOTFSPD 0x0c13 Invalid speed parameter for motor F
ECD_VOOR_TRPIDX 0x0c14 Invalid transport index
ECD_VOOR_FLTIDX 0x0c15 Invalid filter index
ECD_VOOR_FLTUNDEF 0x0c16 Specified filter is not defined
ECD_VOOR_WVLEN 0x0c17 Invalid filter wavelength
ECD_VOOR_CALCMETH 0x0c18 Invalid calculation methods
ECD_VOOR_INVPOSIDX 0x0c19 Invalid positions index
ECD_VOOR_WVLSNRD 0x0c20 Invalid number of measurements during wavelength scan
ECD_VOOR_WVLSDRD 0x0c21 Invalid step distance during wavelength scan
ECD_VOOR_WVLSNWT 0x0c22 Invalid dwell time during wavelength scan
ECD_VOOR_PLTIDX 0x0c50 Invalid disc drive index
ECD_VOOR_PLTUNDEF 0x0c51 Specified disc drive is undefined
ECD_VOOR_PLTNROWS 0x0c52 Invalid number of rows
ECD_VOOR_PLTNCOLS 0x0c53 Invalid number of columns
ECD_VOOR_PLTROWDE 0x0c54 Invalid row definition
F
ECD_VOOR_PLTCOLDEF 0x0c55 Invalid column definition
ECD_VOOR_PLTRDIST 0x0c56 Invalid row distance
ECD_VOOR_PLTCDIST 0x0c57 Invalid column distance
EZ Read 2000 / UVM340 V4.0 44
ECD_VOOR_WELSISTT 0x0c60 Invalid start wave length during wave scan
ECD_VOOR_WELSIEND 0x0c61 Invalid end wave length during wave scan
ECD_VOOR_WELSISGE 0x0c62 Start wavelength of the wave scan is greater than or equal to
the end wavelength
ECD_VOOR_SHKPSINV 0x0c70 The shake position is invalid
ECD_VOOR_SHKSPDINV 0x0c71 One of the pre-set shake speeds is invalid
ECD_VOOR_SHKWAYIN 0x0c72 One of the pre-set paths is invalid
V
ECD_VOOR_SHKTMINV 0x0c73 Shake duration information invalid
ECD_VOOR_SHKSPDIDX 0x0c74 The index for selecting the pre-set shake speed is invalid
ECD_VOOR_SHKWAYID 0x0c75 The index for selecting the pre-set path is invalid
X

EZ Read 2000 / UVM340 V4.0 45

10.0 Preventative Maintenance
The use of the following checklist is at the discretion of the service engineer.
It is intended to help service engineers conduct servicing in a consistent and methodical manner. Copies
of this check list are not formally required for instrument user or Biochrom Ltd.
This section can however be printed, filled out and kept as a record of the service history.

10.1 Details
Institute: Date:
Department: Contract No:
City: Engineer:
Customer name: Instrument:
Telephone: Serial No:
Computer model: Software used:
Software version: Accessories:
Engineer name: Representative name:

Engineer sign: Representative sign:

10.2 Functional tests

Check Notes and Rectification if this fails Pass /
Fail
Ensure that the instrument has been Use the form in Appendix 1; disinfection is
cleaned and decontaminated by the covered in section 1.3.
customer.
Check the condition of the mains power
supply and the mains lead, these should be
safe with no exposed inner insulation.
Check the overall condition of the unit, it
should be safe to use.
Run the QC test plate (as described in Depending on the problem, refer to the
section 6) and obtain a pass. relevant section in the manual.
Perform a portable appliance electrical
safety check on the instrument.
Initial and date a ‘serviced’ sticker and
place it over the existing sticker on the
instrument.

EZ Read 2000 / UVM340 V4.0 46

11.0 Spare Parts list
Description Part Number
Y Motor/Sensor PCB SB019017000
Stepper Motor (Y) (4 wire motor) 80-4000-45
Stepper Motor (X) (6 wire motor) 80-4000-31
Toothed belt X S4110012
Toothed belt Y S4110015
Motor Gear Wheel (X,Y, Mono) (Pack of 3) S5480010000
Pinion S5480009
Fibre optic UVM 340 SB019060
Upper Lens block (with lenses) SB019011000
Lower Lens block (with lenses) SB019010000
Reference LED 580nm S2320105
Monochromator S7400102
Motor for Monochromator SB019043
Spare Lamp SB020009
Y Transport Sensor (Left side with wiring/Large Flag) SB019002
Condenser Lens SB020005
Fibre Optic SB019060
Drive belt monochromator S4110016
CPU Board S6101003
Preamplifier Board SB019065
Interface Board (I/O Main Board) SB019014001
Power Supply 80W S6200121
Fuse 3,15A T - pack 10 S1380022
Reference LED Power Board SB019064
Light regulation Board SB019062
Chassis (Base) SL251009
LED (for top cover) SB018024
Top Cover (UVM340) SL251003
Top Cover assembly complete with overlay (EZ read 2000) 80-4001-94
Wiring for USB/RS-232 SV019042
Cable set (Power) SV019041000
Cable Light control to I/O PCB SV019001001
X Axis sensors and harness SB019019
Preamplifier Cable SV019004
Holmium Filter Calibration Plate 80-4000-05
UVM340 Upgrade (In-House service including calibration) 80-4001-56
UVM340 Upgrade Kit (New Housing, Stray Light Filter) 80-4001-57
QC Plate with WinQC program SG010160
QC Plate Calibration 80-4000-66
RS-232 cable 80-2118-11
Orange Test Plate S5450174
Mains Plug Socket S1542103

EZ Read 2000 / UVM340 V4.0 47

Appendix 1 - DECLARATION OF CONTAMINATION STATUS
Prior to the Inspection, Servicing, Repair or Return of Medical and Laboratory Equipment.

Customer:

Address:

Tel No Fax No:

Instrument Type Serial Number:

Complete section A / C and if applicable complete all of section B, providing further information as requested or appropriate.

A. Has this instrument and its accessories been used in any invasive procedure or been in contact with blood, other body
fluids, respired gases or pathological samples?

YES/NO If YES please complete section B / If NO please proceed to section C.

B. This instrument and its accessories have been exposed internally or externally to hazardous material as indicated below.

YES/NO Blood, body fluids, respired gases, pathological samples, other biohazards.

YES/NO Radiation, chemicals or substances hazardous to health.

YES/NO Other hazards – if YES please specify.

C. Has the instrument and its accessories been cleaned / decontaminated and suitably prepared for safe
handling/transportation?

YES/NO If YES please indicate the methods and materials used for decontamination.

If the instrument and its accessories could not be decontaminated please state the reasons why.

I declare that I have taken all reasonable steps to ensure the accuracy of the above information, in accordance with
MHRA DB 2006(05)

Authorised signature:……………………………………………………………………….

Name (printed):………………………………………………………………………………..

Position:……………………………………………………………………………………………

Date:……………………………………………………………………………………....

Please note that instrumentation will not be accepted for servicing or return until this form is completed fully.
Instrumentation that has not been cleaned sufficiently or decontaminated may be subject to additional charges.

EZ Read 2000 / UVM340 V4.0 48