12/19/2019 Effect of saline purgative on frog intestine - Labmonk

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Effect of saline purgative on Background

Requirements

frog intestine Procedure

Conclusion
References
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Saline purgatives are the salts comprising of highly charged ions and do not
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crosses cell membrane freely. They remain inside the lumen and retain water
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through osmotic forces. They increase the volume of the contents of the
bowel, stretch the colon and produces normal stimulus for contraction of the gene-cloning-plasmids/) Yogi
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muscle that leads to defecation. The aim of the present study is to examine Bacterial growth curve culti
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the effect of saline purgative on frog intestine.
anaerobs
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First Name growth-curve-cultivation-of
REQUIREMENTS anaerobs/) Suman Kumar Meka
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Neuro Humoral Transmissio
Animal: Frog (https://labmonk.com/notes
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Reagents: 0.9% to 0.45% of saline (hypotonic), 27% Magnesium sulphate humoral-transmission/) Sum
(hypertonic), Frogs Ringer solution (isotonic) Mekap

Instruments used: Frog’s board, pithing needle, dissecting instruments, Principles & Mechanism of D
needle with thread, tuberculin syringe with needle. (https://labmonk.com/notes
mechanism-of-drug-action/)
Mekap

PROCEDURE
Pith the frog and place it on a dissecting board. Expose the abdominal cavity
and carefully trace the small intestine. Make the small intestine into three
compartments by tying threads of different colours in such a way that no
fluid can move from one compartment to the other. Inject 0.2 ml of each

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12/19/2019 Effect of saline purgative on frog intestine - Labmonk

hypotonic solution into first compartment, 0.2 ml of hypertonic solution to

second compartment and 0.2 ml of isotonic solution into third compartment.
Wait for 20 minutes and the observations are to be recorded.
OBSERVATION
Buy
Drug Compartment Effect
Health
Hypotonic solution (0.2 ml of First compartment Shrunken Care
0.9% of saline)
Products
Hypertonic solution (0.2 ml of Second Swollen
27% magnesium sulphate) compartment
×
Isotonic solution (0.2 ml of frogs Third compartment No change
Ringer solution)

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Hypotonic solution causes the fluid toclick
moveaway,
fromSubscribe Now
lumen into circulation by at Medlife
process osmosis thereby shrinks the tissue. Hypertonic solution moves the
fluid from cells into the lumen
Firstand swells the tissue and isotonic solution did
Name
not shows any fluid movement across the intestinal membrane.

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REFERENCES
1. Laxative. Available at:
https://www.britannica.com/science/laxative#ref800128. Accessed on
10 November 2016.

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Study of local Study of local
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BACKGROUND Local Local anaesthesia is a activity of drug

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