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Macromolecules Lab

1. The document describes laboratory procedures to test unknown samples for carbohydrates, lipids, and proteins. 2. Tests include Benedict's test for reducing sugars, iodine test for starch, translucence test for lipids, and Biuret test for proteins. 3. Results will be recorded in a data table to analyze reactions and identify the unknown samples.

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Jacob
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134 views

Macromolecules Lab

1. The document describes laboratory procedures to test unknown samples for carbohydrates, lipids, and proteins. 2. Tests include Benedict's test for reducing sugars, iodine test for starch, translucence test for lipids, and Biuret test for proteins. 3. Results will be recorded in a data table to analyze reactions and identify the unknown samples.

Uploaded by

Jacob
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Science 10 Pre-IB

Organic Molecules - “Food Tests”


Testing for Carbohydrates, Lipids, and Proteins Name: Jacob Wu

Problem: Write your own problem. It should include the main “point” of the lab.
(Save this for last).

Background Info: Include a couple of sentences about each test (how they
work, what a positive and negative test should look like-see pre lab sheet)

Materials:
10 test tubes fructose
400 or 600 mL beaker maltose
hot plate sucrose
Benedict’s reagent skim milk
Iodine whole milk
spot plates starch
Biuret reagent glycogen(demo)
unglazed brown paper potato(broth)
thermometer oil
distilled water margarine
plastic pipettes chicken boullion(broth)
Unknowns A – D egg white (albumin)
waste container gelatin
glucose

Procedure: Make sure each sample is well mixed before dispensing into test
tube or spot plates.
Translucence Test for Fat or Lipid
1. Scrape or drop a small portion of sample on unglazed brown paper.
2. Let dry and hold up to the light. Can you see light through the paper?

Benedict’s Test for Reducing Sugars


1. Add approximately 1 ml of each sample (a pipette squirt) and 5 drops of
Benedict’s Reagent to each test tube. Test tube must be clean!!! Record colour
before.
2. Mix and heat in an 80ºC (almost boiling) hot water bath for 1 ½ minutes. Record final
colour.
Iodine test for Starch
1. Add 5 drops of each sample (see data table) to separate spot plate depressions. Make
sure you label spot plate with sample #)
2. Add 3 drops of iodine to each. Record final colour.
Biuret test for Protein
1. Add 5 drops of each sample to separate spot plate depressions. Make sure you
label. Record colour before
2. Add 3 drops of Biuret Reagent to each. Record final colour.
Table 1:

Identification Test Procedure Sample Initial Colour Final Colour


Benedict’s Test for Add approximately Distilled Water
Reducing Sugars 1 mL of sample (a squirt) Glucose
and 5 drops of Benedict’s Fructose
Reagent to a test tube. Sucrose
Mix and heat in an 80ºC Maltose
(almost boiling) hot water Skim milk (lactose)
bath for 1 1/2 minutes. Unknown A
Unknown B
Unknown C
Unknown D
Iodine test for Add 5 drops of a sample Distilled Water
on a spot plate Starch
Starch
depression. Add 2 drops Sucrose
of iodine. Observe final Glycogen
colour with white paper Potato
under the spot plate. Unknown A
Unknown B
Unknown C
Unknown D
Translucence Test Place a small portion of Distilled Water
for Fat or Lipid sample on unglazed Vegetable Oil
brown paper. Let dry Skim Milk
Margarine
and hold up to the light.
Is it translucent or Butter
opaque? Whole milk
Unknown A
Unknown B
Unknown C
Unknown D
Biuret test for To spot plate, add 5 Distilled Water
drops of sample and 3 Albumin (Egg
Protein
drops of Biuret reagent.
White)
Observe final colour with
Chicken Bouillon
white paper placed under
Gelatin
the spot plate.
Skim Milk
Unknown A
Unknown B
Unknown C
Unknown D
Analysis: Design an analysis table that will allow you to answer the problem effectively.(5)

Post Lab Analysis Questions:


1. For each of the tests performed, which sample served as a positive control. Neatly present
your answer. (2)

2. Think of one application for these tests. Describe the circumstances that would be required to
implement one of these tests outside of a science classroom. Your response will require some
research. Cite your references. (2)
Conclusion:

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