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Activity 2 Intro Tissue Sectioning

The document discusses histological techniques for preparing tissue sections. It begins by introducing the different planes used to section tissues and explains how sections are made before microscopic study. The objectives are then stated as differentiating histology and histopathology, discussing common tissue preparation methods, and describing histological stains. The materials used are also listed. The document goes on to provide illustrations of sectioning different specimens like bell pepper and kangkong shoot. It rationalizes making sections and lists important points for microscopic study. Finally, the key tissue preparation methods are outlined as fixation, dehydration, clearing, infiltration, embedding, trimming, and sectioning using a microtome.

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Ericka Genove
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196 views

Activity 2 Intro Tissue Sectioning

The document discusses histological techniques for preparing tissue sections. It begins by introducing the different planes used to section tissues and explains how sections are made before microscopic study. The objectives are then stated as differentiating histology and histopathology, discussing common tissue preparation methods, and describing histological stains. The materials used are also listed. The document goes on to provide illustrations of sectioning different specimens like bell pepper and kangkong shoot. It rationalizes making sections and lists important points for microscopic study. Finally, the key tissue preparation methods are outlined as fixation, dehydration, clearing, infiltration, embedding, trimming, and sectioning using a microtome.

Uploaded by

Ericka Genove
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Group no.

Activity No. 2 Date of Performance: January 31, 2020

Submission of Deadline:
Introduction to Histological
Date: February 3, 2020
Techniques and Tissue Sectioning
Time: 7:30 AM

Instructor: Ms. Angela Lagliva, RMT


Group rating:
CONTENT: NEATNESS:
THIS PORTION WILL BE FILLED BY THE RECEIVING
END:

Date of Submission:
OVERALL: Time of Submission:

INTRODUCTION:
Many views of the body are based on real or imaginary "slieces" called sections or planes. In studying the body or organs,
you often will be observing the flat surface of a section that has been produced by a cut through the body or a body part.
Such sections are made along specific planes. These well- defined planes: transverse, sagittal, and frontal planes, lie at
right angles to each other. It is important to understand the nature of the plane along which a section was made in order to
understand the three-dimensional structure of an object being observed.
Before tissues can be observed under the microscope series of steps are pertformed to prepare these beautifully colored
slides studied to diagnose a disease. The process starts by biopsy or autopsy that enables us to procure the raw specimen
for study. These tissues under study will then be cut in the appropriate section by the pathologist that should fit to the
tissue cassettes in preparation for a more complex histological technique.
The histological techniques used in preparation of such specimens include fixation, dehydration, clearing, embedding,
cutting and staining.

OBJECTIVES:
After accomplishing this activity, the students will be able to:
1. Differentiate Histology and Histopathology.
2. Discuss common methods in the preparation of tissues before mieroscopie study.
3. Discuss the stains used in histologic techniques and give their importance to Histological studies.
4. Demonstrate several ways of making sections.

MATERIALS/RESOURCES:
Reference Book: Laboratory Manual Compiled by Rhealyn Camacho Mostoles; Refer to page 9 Visual Aids
(projector, pictures,videos)
Microscope & Prepared slides
Bring 4 per group: Bell Pepper, Kangkong shoot, Tomato, Kalamansi
Bring 1 per group: Cutting board, Knife and/or Blade

Laboratory Manual in Histology (Arthemae Queenie O. Ciriaco, RMT) 2019


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Activity No. 2
Introduction to Histological Techniques and Tissue Sectioning
WRITE-UP SHEET

I. Illustrate the whole section by means of broken lines, and the sections made on specimen.
Bell Pepper

Whole Longitudinal Section

Transverse Section Oblique Section

Kangkong Shoot

Whole Longitudinal Section

Transverse Section Oblique Section

Laboratory Manual in Histology (Arthemae Queenie O. Ciriaco, RMT) 2019


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Tomato

Whole Longitudinal Section

Transverse Section Oblique Section

Kalamansi

Whole Longitudinal Section

Transverse Section Oblique Section

Laboratory Manual in Histology (Arthemae Queenie O. Ciriaco, RMT) 2019


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2. Rationalize why sections are made in tissues and organs.

Reference/s:

Contributor/s:

3. What are the important points to remember when studying tissue section microscopy?

Reference/s:

Contributors/s:

4. What are the methods used in tissue preparation for microscopic study.
Most tissues studied histologically are prepared as shown, with this sequence of steps
(a):

 Fixation: Small pieces of tissue are placed in solutions of chemicals that preserve by cross-linking proteins and
inactivating degradative enzymes.
 Dehydration: The tissue is transferred through a series of increasingly concentrated alcohol solutions, ending in
100%, which removes all water.
 Clearing: Alcohol is removed in toluene or other agents in which both alcohol and paraffin are miscible.
 Infiltration: The tissue is then placed in melted paraffin until it becomes completely infiltrated with this substance.
 Embedding: The paraffin-infiltrated tissue is placed in a small mold with melted paraffin and allowed to harden.
 Trimming: The resulting paraffin block is trimmed to expose the tissue for sectioning (slicing) on a microtome.
Similar steps are used in preparing tissue for transmission electron microscopy (TEM), except special fixatives and and
embedding involves epoxy resins which become harder than paraffin to allow very thin sectioning.
(b):
A microtome is used for sectioning paraffin-embedded tissues for light microscopy. The trimmed tissue specimen
is mounted in the paraffin block holder, and each turn of the drive wheel by the histologist advances the holder a
controlled distance, generally between 1 and 10 μm. After each forward move, the tissue block passes over the steel knife
edge and a section is cut at a thickness equal to the distance the block advanced. The paraffin sections are placed on glass
slides and allowed to adhere, deparaffinized, and stained for light microscope study. For TEM, sections less than 1 μm
thick are prepared from resin-embedded cells using an ultramicrotome with a glass or diamond knife.

Reference/s: Mescher,A.L. Junqueira’s Basic Histology Text and Atlas. 13 th edition.United States.McGraw Hill Education

Contributors/s: Somera, Danica Rose O.

Laboratory Manual in Histology (Arthemae Queenie O. Ciriaco, RMT) 2019


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