Scribd
Upload
94 views2 pages

Thayer Martin Medium Base: Composition

This document provides information about Thayer Martin Medium Base, which is used for selective isolation of Gonococci from pathological specimens. It lists the composition of the medium and provides directions for preparation and use. It also describes the principles, interpretation, quality control, cultural response and storage requirements.

Uploaded by

Abid Siddiqui
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
94 views2 pages

Thayer Martin Medium Base: Composition

This document provides information about Thayer Martin Medium Base, which is used for selective isolation of Gonococci from pathological specimens. It lists the composition of the medium and provides directions for preparation and use. It also describes the principles, interpretation, quality control, cultural response and storage requirements.

Uploaded by

Abid Siddiqui
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 2

Thayer Martin Medium Base M413

Thayer Martin Medium Base used for selective isolation of Gonococci from pathological specimens.
Composition**
Ingredients Gms / Litre
Peptone, special 23.000
Starch 1.000
Sodium chloride 5.000
Agar 13.000
Final pH ( at 25°C) 7.0±0.2
**Formula adjusted, standardized to suit performance parameters

Directions
Suspend 21.0 grams in 450 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15
lbs pressure (121°C) for 15 minutes. Cool to 45°C. Aseptically add 50 ml of sterile lysed blood and rehydrated contents of one
vial of Vitamino Growth Supplement (FD025) and V.C.N Supplement (FD023) or V.C.N.T Supplement (FD024). If desired
GC Supplement with Antibiotics (FD021) can be used as a single supplement. Mix well before pouring into sterile Petri plates.
If Hemoglobin (FD022) is used suspend 21.0 grams of Thayer Martin Medium Base in 250 ml distilled water.Heat to boiling
to dissolve the medium completely. Prepare 250 ml of 2% hemoglobin solution. Sterilize separately by autoclaving at 15 lbs
pressure (121°C) for 15 minutes. Cool to 45°C. Mix both and add the supplements as above.

Principle And Interpretation


Carpenter and Morton reported an improved medium to isolate Gonococci in 24 hours (1). Later on the efficiency of GC
medium supplemented with haemoglobin and yeast concentrate was demonstrated for isolating gonococci (2). Subsequently
Thayer and Martin Medium was developed for the primary isolation of Neisseria gonorrhoeae and Neisseria meningitidis
from specimens containing mixed flora collected from throat, vagina, rectum an d urethra (3, 4). Thayer and Martin (4) used
Vancomycin, Colistin and Nystatin. Martin and Lester (5) used an additional antibiotic Trimethoprim to make the medium
selective.
Special peptone provides nutrients to the organisms while starch neutralizes the toxic fatty acids if present in the agar.
Haemoglobin provides the X factor whereas the V factor (N.A.D.) is provided by the added supplement. Supplement (FD025)
also supplies vitamins, amino acids, coenzymes etc. which enhances the growth of pathogenic Neisseria . Vancomycin
and colistin inhibits gram-positive and gram-negative bacteria respectively (6). Nystatin inhibits fungi. This medium may
inhibit Haemophilus species. Some strains of Capnocytophaga species may grow on this medium when inoculated
with oropharyngeal specimens

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.3% Agar gel.
Colour and Clarity of prepared medium
Basal Medium : Yellow coloured clear to slightly opalescent gel. After addition of haemoglobin or sterile lysed blood and
supplements: chocolate coloured opaque gel forms in Petri plates.
Reaction
Reaction of 4.2% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response

Please refer disclaimer Overleaf.


HiMedia Laboratories Technical Data

M413: Cultural characteristics observed with added sterile lysed blood/Haemoglobin solution (FD022), Vitamino Growth
Supplement (FD025) and V.C.N. Supplement (FD023)/V.C.N.T. Supplement (FD024) after an incubation at 35-37°C for
18-48 hours.
Organism Inoculum Growth Recovery Colour of
(CFU) colony

Escherichia coli ATCC >=10³ inhibited 0%


25922
Neisseria gonorrhoeae 50-100 good-luxuriant >=50% small, grayish-
ATCC 19424 white to
colourless,
mucoid
Neisseria meningitidis ATCC 50-100 good-luxuriant >=50% medium to
13090 large, blue-
gray, mucoid
Proteus mirabilis ATCC >=10³ inhibited 0%
25933

Storage and Shelf Life


Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.

Reference
1. Carpenter and Morton, 1947, Proc. N.Y. State Assoc. Public Hlth. Labs., 27:58.
2. Carpenter et al, 1949, Am. J. Syphil. Gonorrh. Vener. Dis., 33:164.
3. Martin, Billings, Hackney and Thayer, 1967, Public Hlth. Rep., 82:361.
4. Thayer J. and Martin J.E. Jr., 1966, Public Health Rep., 81:559.
5. Martin J.E. Jr. and Lester A., 1971, HSMHA Hlth. Service Rep., 86(1):30.
6. MacFaddin J., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and
Wilkins, Baltimore.

Revision : 1 / 2011

Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
1919 Email: [email protected]

You might also like