Leucidal® Liquid

Active Micro Technologies

Technical Data Sheet
BACKGROUND Code Number: M15008
Over the past several decades there has been growing public INCI Nomenclature:
pressure, increasingly strict chemical regulations, preservative Leuconostoc/Radish Root Ferment Filtrate
sensitization issues, and the potential for developing micro- INCI Status: Approved
bial resistance to the chemical preservative products typically REACH Status: Fully Compliant
CAS Number: 84775-94-0
used in cosmetic and personal care formulations. These fac-
EINECS Number: 283-918-6
tors have resulted in numerous preservation chemicals be-
Origin: Biotechnology/Botanical:
ing pulled from the marketplace, despite being the products
Leuconostoc kimchii & Raphanus Sativus
of choice at one time. To offer a solution to this preservation
Processing:
paradigm, Active Micro Technologies (AMT) has developed a GMO Free
line of products based on naturally occurring compounds that No Ethoxylation
provide active cosmetic properties, but by their very nature are No Irradiation
also capable of providing product preservation. This antimi- No Sulphonation
crobial capability is due to natural mechanisms developed by No Ethylene Oxide treatment
plants and microorganisms by which they protect themselves No Hydrogenation
from their environment and other competing organisms. Additives: None
-Preservatives: None
SCIENCE -Antioxidants: None
Other additives: None
Leucidal® Liquid is based on an antimicrobial peptide original- Solvents used: Water
ly derived from the lactic acid bacteria, Leuconostoc kimchii. L. Appearance: Clear to Slightly Hazy Liquid
kimchii is one of 15 species of microorgan- Soluble/Miscible: Aqueous Ferment
isms that make up the mixed cul- Extract
ture responsible for producing Suggested Use Levels: 2.0 - 4.0%
Suggested Applications:
the Korean dietary staple
Moisturization, Skin/Scalp Conditioning,
known as kimchi, a type
Antimicrobial
of fermented cabbage.
Like many lactic acid bacteria, L. kimchii is capable of restricting
the growth of other microorganisms by acidifying its environ-
ment, but as is common in nature, it is not content to limit itself to
a single mechanism of defense. In addition to acidifying its envi-
ronment, it also produces a novel antimicrobial peptide. Using modern fermentation and bioprocess-
ing technology, AMT has commercialized this antimicrobial peptide to produce Leucidal® Liquid.
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Active Micro Technologies, LLC - www.activemicrotechnologies.com - [email protected]

107 Technology Drive - Lincolnton, NC 28092 - USA - Phone (704) 276-7100 - Fax (704) 276-7101
Information contained in this technical literature is believed to be accurate and is offered in good faith for the benefit of the customer. The company, however, cannot assume any liability or risk involved in the use of its chemi-
cal products since the conditions of use are beyond our control. Statements concerning the possible use of our products are not intended as recommendations to use our products in the infringement of any patent. We make
no warranty of any kind, expressed or implied, other than that the material conforms to the applicable standard specification. Freedom from patent infringement is not implied. All information is for investigative purposes only.
 Leucidal® Liquid Leucidal® Liquid
Active Micro Technologies Active Micro Technologies
The first step in the development of this product was to de- Microorganism Tested MIC (%) BENEFITS
termine the peptide’s potential ability to inhibit the growth E. coli 1.60
of a variety of bacteria and fungi. Using standard serial dilu- P. aeruginosa 0.80 Because peptides have been found to provide skin moisturization properties, Leucidal® Liquid was
tion protocols in growth media, the Minimum Inhibitory Con- S. aureus 1.60 used in a comparative study to evaluate its ability to provide cosmetic benefits, in addition to the dem-
centrations (MICs) for Leucidal® Liquid were determined for C. albicans 0.80 onstrated antimicrobial function. A skin moisturization study was performed using a generic cream
a variety of both bacterial and fungal organisms. The results A. niger 2.40 base, compared with the same cream base containing 1% Leucidal® Liquid. As demonstrated by the
of these tests are shown in Table 1. Table 1. MIC Data for Leucidal® Liquid results of this study, shown in Table 3, the addition of 1% Leucidal® Liquid to the base cream formula-
tion provided a 10% increase in moisturization. Based on these results, adding this innovative prod-
The positive MIC screening results warranted further testing to confirm its ability to provide prod- uct provides the formulator the opportunity to capitalize on both the natural antimicrobial properties of
uct preservation. A Double Challenge Test was completed using 2% Leucidal® Liquid in a gener- Leucidal® Liquid, as well as its ability to provide potent moisturizing benefits to the cosmetic formula-
ic cream base formulation. Samples were inoculated with E. coli, P. aeruginosa, S. aureus, K. pneu- tion. These properties make it ideal for applications addressing numerous skin and scalp conditions.
moniae, C. albicans, A. niger, and B. cepacia. During the first 28-day incubation period, samples were
periodically collected and tested for the presence of these microorganisms. Following this initial 28 Moisturization Results
days of incubation, the cream samples were then re-inoculated with the microbial cultures and sampled 104
over an additional 28-day period. Table 2 shows the positive preservation results for Leucidal® Liquid. 102

Average Impedance Value

E. coli P. aeruginosa S. aureus K. pneumoniae C. albicans B. cepacia 100
A. niger
98
Inoculum
Level (initial) 2.37E+06 2.33E+06 2.02E+06 2.79E+06 9.46E+05 2.97E+05 1.98E+06 96

94
Day 0 5.485% 28.755% +1.485% 6.810% 39.535% 87.542% >99.999%
Day 1 >99.999% >99.999% >99.999% >99.999% >99.999% 90.404% >99.999% 92
Day 2 >99.999% >99.999% >99.999% >99.999% >99.999% 92.256% >99.999% 90
Day 3 >99.999% >99.999% >99.999% >99.999% >99.999% 94.108% >99.999% 88
Day 7 >99.999% >99.999% >99.999% >99.999% >99.999% 99.663% >99.999%
86
Day 14 >99.999% >99.999% >99.999% >99.999% >99.999% 99.663% >99.999%
Week 2 Week 3 Week 4
Day 21 >99.999% >99.999% >99.999% >99.999% >99.999% 99.789% >99.999%
Time
Day 28 >99.999% >99.999% >99.999% >99.999% >99.999% 99.987% >99.999%
Inoculum
Control AMS Leucidal Liquid
level (re- 2.57E+06 2.212E+06 1.16E+06 1.23E+06 7.03E+06 2.48E+05 2.18E+06
inoculated) Table 3. Increase in Moisturization for Leucidal® Liquid

Day 0 38.132% 42.453% 50.690% 7.317% 37.127% 13.306% >99.999%

Day 1 >99.999% >99.999% >99.999% >99.999% >99.999% 17.339% >99.999% USE RECOMMENDATIONS
Day 2 >99.999% >99.999% >99.999% >99.999% >99.999% 37.500% >99.999%
Day 3 >99.999% >99.999% >99.999% >99.999% >99.999% 84.879% >99.999% As with all biological materials, some attention must paid to the conditions under which Leucidal® Liquid
Day 7 >99.999% >99.999% >99.999% >99.999% >99.999% 94.758% >99.999% is used. Based on bench-scale evaluations, as well as actual product applications, Leucidal® Liquid has
Day 14 >99.999% >99.999% >99.999% >99.999% >99.999% 96.371% >99.999% been found to be effective over a wide range of typical cosmetic and personal care product manufacturing
Day 21 >99.999% >99.999% >99.999% >99.999% >99.999% 96.371% >99.999% conditions. The product has been found to be heat stable up to 70°C and active under both acidic (pH 3)
Day 28 >99.999% >99.999% >99.999% >99.999% >99.999% 99.113% >99.999% and basic conditions (pH 8).
Table 2. Challenge Test results for 2% Leucidal® Liquid in O/W emulsion inoculated on day 0 and re-inoculated on day 28.
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Active Micro Technologies, LLC - www.activemicrotechnologies.com - [email protected] Active Micro Technologies, LLC - www.activemicrotechnologies.com - [email protected]
107 Technology Drive - Lincolnton, NC 28092 - USA - Phone (704) 276-7100 - Fax (704) 276-7101 107 Technology Drive - Lincolnton, NC 28092 - USA - Phone (704) 276-7100 - Fax (704) 276-7101
Information contained in this technical literature is believed to be accurate and is offered in good faith for the benefit of the customer. The company, however, cannot assume any liability or risk involved in the use of its chemi- Information contained in this technical literature is believed to be accurate and is offered in good faith for the benefit of the customer. The company, however, cannot assume any liability or risk involved in the use of its chemi-
cal products since the conditions of use are beyond our control. Statements concerning the possible use of our products are not intended as recommendations to use our products in the infringement of any patent. We make cal products since the conditions of use are beyond our control. Statements concerning the possible use of our products are not intended as recommendations to use our products in the infringement of any patent. We make
no warranty of any kind, expressed or implied, other than that the material conforms to the applicable standard specification. Freedom from patent infringement is not implied. All information is for investigative purposes only. no warranty of any kind, expressed or implied, other than that the material conforms to the applicable standard specification. Freedom from patent infringement is not implied. All information is for investigative purposes only.