Course Course name L-T-P-Credits Year of

code Introduction
AE402 ANALYTICAL INSTRUMENTATION 3-0-0-3 2016
Prerequisite : Nil
Course objectives
 To review background information required for studying virtual instrumentation.
 To study the basic building blocks of virtual instrumentation.
 To study the various graphical programming environment in virtual instrumentation.
 To study a few applications in virtual instrumentation.
Syllabus
Fundamentals of analytical instruments –Classification of instrumental techniques -
Electromagnetic radiation- Electromagnetic spectrum- Absorption spectroscopy - Ultra violet
and visible absorption spectroscopy - Colorimeters/ photometers - Spectro photometers -
Infra red spectroscopy - Atomic absorption spectrophotometers - Fluorescence spectroscopy -
Raman spectrometer - Mass spectrometer - Nuclear Magnetic Resonance spectroscopy -
Electron spectroscopy - X- Ray spectrometers - Chromatographic process – Classification -
Gas chromatography - Liquid Chromatography - High pressure Liquid Chromatography -
Industrial Gas analysers - Gas analysers - Blood PH measurement – Thin film technology for
gas sensors- Thermal Sensors.
Expected outcome
 At the end of the semester students will be able to obtain comprehensive knowledge
in analytical instrumentation and some of its applications.
Text Books
1. Skoog, Holler, Nieman, “Principles of Instrumental Analysis”, Thomson books-cole
publications, 5th edition.
2. Willard, Merritt, Dean, Settle , “Instrumental Methods of Analysis”, CBS Publishers
& Distributors, New Delhi, Seventh edition.
.
Reference Books
1. Galen W. Ewing, “Instrumental Methods of Chemical Analysis”, , McGraw-Hill
Book Company, Fifth edition.
2. R. S. Khandpur , “Handbook of Analytical Instruments”, , Tata McGraw–Hill
Publications, 3rd edition.
3. Robert D. Braun, “Introduction to Instrumental Analysis”, , McGraw-Hill Book
Company
Course Plan
Semester
Module Contents Hours Exam
Marks
I Introduction to Analytical Instrumentation: Fundamentals of 6 15%
analytical instruments: Elements of an analytical instrument
– PC based analytical instruments –Classification of
instrumental techniques. Electromagnetic radiation-
Electromagnetic spectrum- Laws relating to absorption of
radiation. Absorption spectroscopy: Absorption instruments
– Radiation sources- Optical filters- Monochromators-
Detectors. Ultra violet and visible absorption spectroscopy.
II Colorimeters/ photometers: Single beam and double beam 7 15%
filter photometer – Spectro photometers: Single beam and
 double beam spectro photo meters- Infra red spectroscopy:
Basic components- Radiation sources- Monochromators-
Detectors. Flame Photometry: Principle and constructional
details of flame photometer- Emission system – Optical
system – Detectors. Atomic absorption spectrophotometers:
Theoretical concepts, Instrumentation: Radiation sources -
Burners and flames - Plasma excitation sources - Optical
and electronic system.
FIRST INTERNAL EXAMINATION
III Fluorescence spectroscopy: Principle of fluorescence – 7 15%
Measurement of fluorescence – Single beam and double
beam filter fluorimeter- Ratio fluorimeter. Spectro
fluorimeters. Raman spectrometer- Basic theory-Photo
acoustic spectroscopy- Photo thermal spectroscopy. Mass
spectrometer: Principle of operation- Magnetic deflection
mass spectrometers- Components of a mass spectrometer –
Inductively coupled plasma mass spectrometer.
IV Nuclear Magnetic Resonance spectroscopy: Basic principle 7 15%
– Constructional details of NMR spectrometer – Nuclear
radiation detectors. Electron Spin Resonance spectrometer:
Basic ESR spectrometer – Electron spectroscopy:
Instrumentation for electron spectroscopy. X- Ray
spectrometers: X – ray spectrum –Instrumentation for x –ray
spectrometry. X-ray diffractometers- X-ray absorption
meters- X- ray fluorescence spectrometry.

SECOND INTERNAL EXAMINATION

V Chromatography: Chromatographic process – 7 20%
Classification- Terms in chromatography- Gas
chromatography: Block diagram- Principle - Constructional
details – Column details- GC detectors. Liquid
Chromatography: Types of liquid chromatography- High
pressure Liquid Chromatography (HPLC): Principle-
Constructional details.
VI Industrial Gas analyzers- pH meters- Conductivity meters - 8 20%
Dissolved oxygen meters- Sodium analyser– Gas analysers-
Paramagnetic oxygen analyser – CO analysers – Flue gas
analysers- Blood PH measurement – Thin film technology
for gas sensors- Basic concepts. Measurement techniques
and application of gas sensors. Thermal Sensors:- Radiation
Sensors, Mechanical Sensors and Bio-Chemical sensors.
END SEMESTER EXAMINATION
QUESTION PAPER PATTERN:

Maximum Marks:100 Exam Duration: 3 Hours

Part A

Answer any two out of three questions uniformly covering Modules 1 and 2 together. Each
question carries 15 marks and may have not more than four sub divisions.

(15 x 2 = 30 marks)

Part B

Answer any two out of three questions uniformly covering Modules 3 and 4 together. Each
question carries 15 marks and may have not more than four sub divisions.

(15 x 2 = 30 marks)

Part C

Answer any two out of three questions uniformly covering Modules 5 and 6 together. Each
question carries 15 marks and may have not more than four sub divisions.

(20 x 2 = 40 marks)