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Experiment 9 - Vitamin Analysis PDF

This document describes an experiment analyzing various vitamins. It discusses the two classes of vitamins - water-soluble and fat-soluble vitamins. The experimental results section details preparations and analyses for several vitamins, including using carbon tetrachloride to dissolve fat-soluble vitamins, detecting vitamin A using antimony trichloride to form a blue complex, and identifying vitamin C by observing the reduction of phosphomolybdic acid to molybdenum blue. The analyses aim to identify the vitamins through characteristic color changes or precipitate formations in specific chemical reactions.

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AALIYAH REIGN MAPUSAO
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171 views14 pages

Experiment 9 - Vitamin Analysis PDF

This document describes an experiment analyzing various vitamins. It discusses the two classes of vitamins - water-soluble and fat-soluble vitamins. The experimental results section details preparations and analyses for several vitamins, including using carbon tetrachloride to dissolve fat-soluble vitamins, detecting vitamin A using antimony trichloride to form a blue complex, and identifying vitamin C by observing the reduction of phosphomolybdic acid to molybdenum blue. The analyses aim to identify the vitamins through characteristic color changes or precipitate formations in specific chemical reactions.

Uploaded by

AALIYAH REIGN MAPUSAO
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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EXPERIMENT 9:

VITAMIN
ANALYSIS
VITAMINS
Organic compound essential for proper
body functioning.
Obtained from the dietary sources
Cofactor in conjugated enzymes

2 2
VITAMINS
Two classes of Vitamins:
• Water-soluble Vitamins
• Fat (lipid) soluble Vitamins

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1. WATER-SOLUBLE VITAMINS:
Vitamin C (Ascorbic acid)
Vitamin B Complex: (B1 to B12)
• Vitamin B1 (Thiamine)
• Vitamin B2 (Riboflavin)
• Vitamin B3 (Niacin)
4 4
2. FAT-SOLUBLE VITAMINS:
Vitamin A (Retinol, β-carotene)
Vitamin D (Calciferol)
Vitamin E (Tocopherol)
Vitamin K (Phylloquinone/
Menaquinone)

5 5
EXPERIMENTAL
RESULTS:

6
A. PREPARATION FROM
COMMERCIALLY-PREPARED
VITAMINS
CCl4 (Carbon Tetrachloride) is used to
dissolve fat-soluble Vitamins (A, D, E).
A nonpolar solution.
7
B. ORGANIC SOURCES
Carrots – source of Vitamin A
Fish liver oil – source of Vitamin D
Coconut oil – source of Vitamin E
Yeast – source of Vitamin B3 (Niacin)
Tomatoes – source of Vitamin C

8
C. VITAMIN A ANALYSIS
SbCl3 (Antimony Trichloride) in
Chloroform is used to detect Vitamin A
or carotenoids.
Colorimetric method: “Carr-Price
Method”
Positive result: Blue-colored complex
9
D. VITAMIN D ANALYSIS
Pyrogallol in Benzene – used to dissolve
Vitamin D.
AlCl3 (Aluminum Chloride) – inhibits
Vitamin D – dependent Calcium
absorption.

10
D. VITAMIN D ANALYSIS
Principle: Aluminum will form a complex
with Vitamin D, inhibiting it from being
absorbed by the body.
Result: Yellow solution with white
precipitate

11
E. VITAMIN E ANALYSIS
Nitric acid in alcohol is a very explosive
solution.
Positive result: Formation of 2 layers.
Top layer – orange-colored solution
Bottom layer – yellow –colored solution

12
F. NIACIN ANALYSIS
NaOH – used to neutralize the solution
Copper (III) Sulfate – used to dissolve
Vitamin B
Result: Deep blue solution

13
G. VITAMIN C ANALYSIS
Phosphomolybdic acid is reduced into
Molydenum Blue by Ascorbic acid.
Color change is directly proportional to
the amount of Vitamin C present.
Result: Blue-colored complex

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