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4.2 Perhitungan Lap p2

The document describes the process of calculating the calibration curve and linear regression equation for determining flavonoid content. Different concentrations of 9-24 ppm were used to generate the calibration curve. Absorbance readings from a sample were then used to calculate flavonoid content via the linear regression equation. The average flavonoid content across three trials was determined to be 3.5462%.

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27 views3 pages

4.2 Perhitungan Lap p2

The document describes the process of calculating the calibration curve and linear regression equation for determining flavonoid content. Different concentrations of 9-24 ppm were used to generate the calibration curve. Absorbance readings from a sample were then used to calculate flavonoid content via the linear regression equation. The average flavonoid content across three trials was determined to be 3.5462%.

Uploaded by

Dwi Wulan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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4.

2 Perhitungan

4.2.1 Perhitungan pembuatan kurva kalibrasi

25 mg
Larutan induk => =1000 ppm
25 ml

a. 9 ppm => 1000 x V1 = 9 x 10

V1 = 0.09 ml

b. 12 ppm => 1000 x V1 = 12 x 10

V1 = 0.12 ml

c. 15 ppm => 1000 x V1 = 15 x 10

V1 = 0.15 ml

d. 18 ppm => 1000 x V1 = 18 x 10

V1 = 0.18 ml

b. 21 ppm => 1000 x V1 = 21 x 10

V1 = 0.21 ml

b. 24 ppm => 1000 x V1 = 24 x 10

V1 = 0.24 ml

4.2.2 Persamaan regresi linier

Konsentrasi Absorbansi a = -0.026


(µg/ml)
b = 0.036
9 0.299
12 0.405 r = 0.998

15 0.514 persamaan regresi linier

18 0.597 y = bx + a
21 0.732 y = 0.036x -0.026
24 0.837
4.2.3 Kadar Flavonoid (%)

Nilai absorbansi sampel dilakukan tiga kali pengulangan : 0.607; 0.620 dan 0.610

10
Faktor pengenceran : =2
5

Konsentrasi sampel :

a. absorbansi 0.607

y = 0.036x - 0.026

0.607 = 0.036x - 0.026

X = 17.583 ppm

b. absorbansi 0.620

y = 0.036x - 0.026

0.620 = 0.036x - 0.026

X = 17.944 ppm

c. absorbansi 0.610

y = 0.036x - 0.026

0.610 = 0.036x - 0.026

X = 17.666 ppm

Kadar flavonoid 1

C(µg /ml)x V ekstrak (ml) x faktor pengenceran x 100 %


kadar flavonoid=
berat sampel( mg)

17.583 10
mg/ml x 25 ml x x 100 %
1000 5
kadar flavonoid=
25 mg

kadar flavonoid=3.5166 %

Kadar flavonoid 2
C(µg /ml)x V ekstrak (ml) x faktor pengenceran x 100 %
kadar flavonoid=
berat sampel( mg)

17.944 10
mg/ml x 25 ml x x 100 %
1000 5
kadar flavonoid=
25 mg

kadar flavonoid=3.5888 %

Kadar flavonoid 3

C(µg /ml)x V ekstrak (ml) x faktor pengenceran x 100 %


kadar flavonoid=
berat sampel( mg)

17.666 10
mg/ml x 25 ml x x 100 %
1000 5
kadar flavonoid=
25 mg

kadar flavonoid=3.5332 %

kadar 1+kadar 2+ kadar 3


kadar flavonoid rata−rata(%)=
3

3.5166+3.5888+ 3.5332
kadar flavonoid rata−rata(%)=
3

kadar flavonoid rata−rata(%)=3.5462

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