Botany II LAB
Botany II LAB
* The plant body is thalloid which shows three distinct regions i.e. the epidermal region, the
photosynthetic region and the storage region.
*The epidermal region is formed by quadrate cells with few chloroplastids.
*The photosynthetic region is horizontal row full of chloroplastids arise from the floor of the air
chamber.
* The storage region: The ventral tissue lays immediately below the air chambers forms the storage
region. 1 Mark
OR
A.Anthoceros- 3 Marks
* The given specimen is identified as “Anthoceros agrestis”. 1 Mark
* The thallus is comprised of uniform, thin walled parenchymatous cells except the epidermis.
* The thallus is several layers thick in the middle and tapers towards the margins.
*Each cell of the thallus shows one or more discoid or oval chloroplasts containing many
pyrenoids.
*There are no air chambers or pores in the tissue of the thallus. 1 Mark
OR
A. Polytrichum- 3 Marks
* The given specimen is identified as “Polytrichum alpinum”. 1 Mark
OR
B. Equisetum
* The given specimen is identified as “Equisetum fluviatile (River horse tail)”. 1 Mark
C – Pteridophyta- 3 Marks
C. Ophioglossum
* The given specimen is identified as “Ophioglossum polyphyllim”. 1 Mark
D – Gymnosperms- 3 Marks.
D. Cycas
* The given specimen is identified as “Cycas rumphii”. 1 Mark
2 Marks
* Branched or Unbranched trunks.
* Foliage (Pinnate) and Scaly (Bi-Pinnate) leaves.
*Tap roots and coralloid roots.
*Stem thick, woody and usually un-branched. 1 Mark
OR
D. Pinus
* The given specimen is identified as “Pinus roxburghii”. 1 Mark
2 Marks
* Leaves are needle like, in fascicles of three.
*The bark is red-brown, thick and deeply fissured at the base of the trunk.
* Roots-Deep tap roots and radial surface roots.
* Cones are ovoid conic.
* It is monoceious. 1 Mark
OR
D. Gnetum
* The given specimen is identified as “Gnetum montanum”. 1 Mark
2 Marks
*Young roots have several layers of starch filled parenchymatous cortex.
* Stem is roughly circular and resembles dicotyledonous stem.
* Leaves also resemble dicotyledons.
2 Marks
*It is wavy in outline because of the presence of ridges and grooves.
* Outermost layer is the epidermis, cells of which have a deposit of
silica in their outer and lateral walls.
*Due to the presence of silica, the stem appears hard and rough to touch.
*Below the epidermis is present a-well-developed cortex.
*It has “V” shaped Vascular bundle. 1 Mark
OR
E. Anatomy of Selaginella stem
* The given specimen is identified as “T.S. of Selaginella stem”. 1 Mark
2 Marks
*The stem is composed of epidermis, cortex and central stele.
* Epidermis is the outermost layer without stomata.
2 Marks
*Outermost layer is epidermis, which consists of thick-walled cells. It is covered by a very strong
cuticle.
*Many sunken stomata are present on the epiderm.
*Below the epidermis are present a few layers of thick-walled sclerenchymatous hypodermis. It is
well-developed at ridges.
*In between the hypodermis and endodermis is present the mesophyll tissue.
*Cells of the mesophyll are polygonal and filled with chloroplasts. Many peg-like infoldings of
cellulose also arise from the inner side of the wall of mesophyll cells.
*Endodermis is single-layered with barrel-shaped cells and clear casparian strips.
*Xylem lies towards the angular side and the phloem towards the convex side of the needle. 1 Mark
3. Mount the given specimen G and Identify giving reasons- Pinus pollen grains 05
* The given specimen is identified as “T.S. of Pinus needle”. 1 Mark
2 Marks
*A male cone consists of a large number of microsporophylls arranged spirally on the cone axis.
*A microsporophyll tip is projected upwards and called apophysis.
*Each microspore or pollen grain is a rounded and yellow-coloured, light, uninucleate structure
with two outer coverings, i.e., thick outer exine and thin inner intine.
*The exine protrudes out on two sides in the form of two balloon-shaped wings. Wings help in
floating and dispersal of pollen grains.
*Wings help in floating and dispersal of pollen grains.