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Its Bean A Blast Lab PDF

This experiment tests whether germinating seeds produce carbon dioxide by placing soaked beans or no beans in jars with bromothymol blue solution. Bromothymol blue changes color based on pH, with neutral solutions being blue and acidic solutions being yellow. It is predicted the jar with beans will turn yellow as the germinating seeds produce carbon dioxide, lowering the pH and changing the color of the indicator. Observations of the jar colors will be recorded daily in a data table.

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Poy Alis
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42 views

Its Bean A Blast Lab PDF

This experiment tests whether germinating seeds produce carbon dioxide by placing soaked beans or no beans in jars with bromothymol blue solution. Bromothymol blue changes color based on pH, with neutral solutions being blue and acidic solutions being yellow. It is predicted the jar with beans will turn yellow as the germinating seeds produce carbon dioxide, lowering the pH and changing the color of the indicator. Observations of the jar colors will be recorded daily in a data table.

Uploaded by

Poy Alis
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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CARBON DIOXIDE PRODUCTION BY GERMINATING SEEDS

MATERIALS:

50 beans 1 test tubes or small beaker


1 one-quart jars with airtight lids Bromothymol blue solution
Beaker or small jar to soak seeds Water
Paper towels

PROCEDURE:

1. Obtain 1 jar and place several layers of moist paper towels in the bottom.

2. Half of the lab groups will place 50 pre-soaked (the night before) beans in your jar. The
other half will not use beans. Mrs. Evans will tell you which group you are in.

3. Fill 1 small beaker halfway with Bromothymol blue and place it in the center of your jar.
Nestle beans around jar (if you have beans).

4. Place the lids on the jars tightly and keep the jars on the windowsill.

5. Observe jars the next day and record data in the DATA TABLE. Remember, the
observation after the jars are set-up, is for day zero.

DATA TABLE: Carbon Dioxide Production by Germinating Seeds


Date Jars with Beans Jar without Beans
Day Zero blue blue

HYPOTHESIS/PREDICTION
HYPOTHESIS/PREDICTION:
/PREDICTION: Create a hypothesis for this lab. What do you think will happen in each of the
jars? Explain.

Questions: (Students should know that bromothymol blue reacts (changes color) depending on the pH. The jars
that are neutral should be blue because water is neutral. When CO2 is added to the bromothymol blue
and water solution, the pH is decreased as the CO2 is dissolved slightly, and carbonic acid is formed.
This slightly acidic solution becomes yellow.)

1. Why do we use bromothymol blue as an indicator?

2. Which of the jars showed change? What change was seen? Explain what caused this change.

Conclusion: Did you accept or reject your hypothesis? Explain your reasons to support your answer.

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