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Tributyrin Agar, Base: Mode of Action Literature

Tributyrin Agar, Base is a culture medium proposed by Anderson in 1939 for detecting and enumerating lipolytic microorganisms in food and other materials. The medium contains tributyrin, which lipolytic colonies degrade to form clear zones in the otherwise turbid agar. When inoculated and incubated, lipolytic bacteria will form colonies surrounded by clear zones, allowing them to be detected and enumerated. The medium has been used to detect lipases in various bacteria like staphylococci, clostridia, Pseudomonas, and marine flavobacteria.

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105 views1 page

Tributyrin Agar, Base: Mode of Action Literature

Tributyrin Agar, Base is a culture medium proposed by Anderson in 1939 for detecting and enumerating lipolytic microorganisms in food and other materials. The medium contains tributyrin, which lipolytic colonies degrade to form clear zones in the otherwise turbid agar. When inoculated and incubated, lipolytic bacteria will form colonies surrounded by clear zones, allowing them to be detected and enumerated. The medium has been used to detect lipases in various bacteria like staphylococci, clostridia, Pseudomonas, and marine flavobacteria.

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Tributyrin Agar, Base

Medium proposed by ANDERSON (1939) for the detection and enumeration of lipolytic microorganisms in foodstuffs
and other materials. The medium can also be used for the detection of lipase in various bacterial species such as
staphylococci (INNES 1956), clostridia (WILLIS 1960), Pseudomonas, marine flavobacteria (HAYES 1963) etc.
Tributyrin Agar, Base

Mode of Action Literature


The culture medium contains tributyrin as a reactant; degrada- ANDERSON, J.A.: The use of tributyrin agar in dairy bacteriology. - Ber. 3.
tion of this compound gives rise to clear zones surrounding the Int. Mikrobiol. Kongress, 3 ; 726-728 (1939)
lipolytic colonies in the otherwise turbid culture medium. EL SADEK, G.M., a. RICHARDS, T.: Nile blue, aniline blue and neutral red as
indicators of lipolysis. - J. Appl. Bact., 20; 137 (1959).
Typical Composition (g/litre) INNES, A.G.: Coagulase positive Staphylococci from bulk milk supplies low
Peptone from meat 2.5; peptone from casein 2.5; yeast extract in solids-notfat. - J. Appl. Bact., 19; 39-45 (1956).
3.0; agar-agar 12.0. HAYES, P.R.: Studies on marine flavobacteria. - J. Gen. Microbiol., 30; 1-19
(1963).
Also to be added:
RAPP, M.: Elektive Nährmedien zum Nachweis von Lipolyten. - Milchwirt-
Tributyrin 10.0 ml. sch., 33; 493-496 (1978).
Preparation WILLIS, A.T.: The lipolytic activity of some clostridia. - J. Path. Bact., 80;
379-390 (1960).
Suspend 20 g/litre, add 10 ml neutral tributyrin/litre, mix
uniformly and autoclave (15 min at 121 °C). While shaking Ordering Information
frequently (emulsification of the tributyrin) cool to at least 50°C
(stabilization of the emulsion) and pour plates. Allow the plates Product Merck Cat. No. Pack size
to solidify rapidly. Tributyrin Agar, Base 1.01957.0500 500 g
pH: 7.5 ± 0.2 at 25 °C. Glycerol tributyrate 1.01958.0100 100 ml
The plates are turbid and yellowish. (Tributyrin)
n The culture medium must contain a uniformly turbid
emulsion. If the emulsion separates, the effectiveness of
the culture medium is affected. Manufacturer Product
EL SADEK and RICHARDS (1957) reported that other glycerides ICI Chemicals, Essen, BRD Polyoxyethylene-(20)-hydrated
such as triolein and trilinolein can be used instead of tributyrin. Ricinus oil
According to RAPP (1978), better emulsification of tributyrin
can be achieved if 4 ml polyoxyethylene-(20)-hydrated ricinus
oil is added to 1 litre of the culture medium.

Experimental Procedure and Evaluation


Inoculate the culture medium by the pour-plate method or by
spreading the sample material on the surface of the plates.
Incubation: up to 72 hours under optimal conditions (e.g. 28 °C).
Lipolytic microorganisms produce colonies which are sur-
rounded by clear zones in the otherwise turbid culture medium.

Quality control
Test strains Growth Clear zones
Escherichia coli ATCC 25922 good / very good -
Salmonella typhimurium ATCC 14028 good / very good -
Pseudomonas aeruginosa ATCC 27853 good / very good +
Staphylococcus aureus ATCC 25923 good / very good +
Bacillus subtilis ATCC 6633 good / very good +
Penicillium commune ATCC 10428 poor / fair +

470 Merck Microbiology Manual 12th Edition

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