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Determination of Acid Value of Fats

The document defines acid value as the milligrams of potassium hydroxide needed to neutralize the free fatty acids in one gram of fat. It describes a procedure to determine acid value which involves dissolving a fat sample in a solvent, adding an indicator, and titrating with potassium hydroxide solution until a pale pink endpoint is reached. The acid value is then calculated based on the volume and molarity of potassium hydroxide used and the weight of the fat sample, with acceptable levels below 0.6 mg KOH/g.

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178 views1 page

Determination of Acid Value of Fats

The document defines acid value as the milligrams of potassium hydroxide needed to neutralize the free fatty acids in one gram of fat. It describes a procedure to determine acid value which involves dissolving a fat sample in a solvent, adding an indicator, and titrating with potassium hydroxide solution until a pale pink endpoint is reached. The acid value is then calculated based on the volume and molarity of potassium hydroxide used and the weight of the fat sample, with acceptable levels below 0.6 mg KOH/g.

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DETERMINATION OF ACID VALUE OF FATS

Acid value is defined as the number of milligrams of potassium


hydroxide required to neutralise the free fatty acids in one gram of fat.
Procedure
 100 mL of fat solvent was prepared by mixing 50 mL of absolute
ethanol and 50 mL of diethyl ether in a beaker.
 Covering the solution is required due to its volatile trait.
 Then, 1.0 mL of fat sample or used cooking oil was dissolved in 10
mL of fat solvent.
 Next, 2 drops of phenolphthalein indicator was added and it was
mixed thoroughly.
 It was titrated against 0.01 M KOH until a pale pink end point is
achieved.

𝑎𝑐𝑖𝑑 𝑣𝑎𝑙𝑢𝑒 = 𝑣𝐾𝑂𝐻 × 𝑀𝐾𝑂𝐻 × 𝑀𝑊𝐾𝑂𝐻(𝑚𝑔)


𝑤𝑒𝑖𝑔ℎ𝑡 𝑜𝑓 𝑓𝑎𝑡 (𝑔)

Acceptable levels for all oil samples should be below 0.6 mg KOH/g

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