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Data Pengamatan 1. Perhitungan Larutan Induk

1. The document details the preparation of stock solutions of different concentrations from a 1000 ppm parent solution to create a calibration curve. 2. Absorbance readings were taken over time for samples and used to calculate flux values through a diffusion membrane. 3. The maximum flux value was determined to be 0.0424 mg/cm2 at 60 minutes, and the percentage of drug diffused over 120 minutes was calculated to be 5.325% of the initial dose.

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Farah Yumna Ambaro
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21 views

Data Pengamatan 1. Perhitungan Larutan Induk

1. The document details the preparation of stock solutions of different concentrations from a 1000 ppm parent solution to create a calibration curve. 2. Absorbance readings were taken over time for samples and used to calculate flux values through a diffusion membrane. 3. The maximum flux value was determined to be 0.0424 mg/cm2 at 60 minutes, and the percentage of drug diffused over 120 minutes was calculated to be 5.325% of the initial dose.

Uploaded by

Farah Yumna Ambaro
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Data Pengamatan

1. Perhitungan Larutan Induk


Larutan induk yang dibuat adalah 1000 ppm dalam 10 mL, maka zat aktif
yang ditimbang adalah 1000 ppm = 1000 μg/mL
1000 μg
10 mL → x 10 mL = 10000 μg = 10 mg
1 mL

2. Perhitungan Larutan Stok


7,5 ppm → V1 . N1 = V2 . N2
V1 . 1000 ppm = 10 mL . 7,5 ppm
V1 = 0,075 ppm
10 ppm → V1 . N1 = V2 . N2
V1 . 1000 ppm = 10 mL . 10 ppm
V1 = 0,1 ppm
12,5 ppm → V1 . N1 = V2 . N2
V1 . 1000 ppm = 10 mL . 12,5 ppm
V1 = 0,125 ppm
15 ppm → V1 . N1 = V2 . N2
V1 . 1000 ppm = 10 mL . 15 ppm
V1 = 0,15 ppm
17,5 ppm → V1 . N1 = V2 . N2
V1 . 1000 ppm = 10 mL . 17,5 ppm
V1 = 0,175 ppm
20 ppm → V1 . N1 = V2 . N2
V1 . 1000 ppm = 10 mL . 20 ppm
V1 = 0,2 ppm

3. Data Kurva Kalibrasi


Konsentrasi (ppm) Absorbansi (A)
7,5 0,331
10 0,451
12,5 0,6
15 0,718
17,5 0,749
20 0,834

Tabel 1. Data Kurva Kalibrasi


a = 0.064 b = 0,040 r = 0,978
y = 0,040x + 0,064

4. Data Absorbansi Sampel


Waktu (menit) Absorbansi (A)
15 0,356
30 0,378
60 0,338
90 0,214
120 0,134
Tabel 2. Data Absorbansi Sampel

5. Perhitungan Sampel Uji


y = 0,040x + 0,064
t15 → 0,356 = 0,040 x + 0,064
x0,356−0,064
=
0,040
x = 7,3 x 15 = 109,5 μg
t30 → 0,378 = 0,040 x + 0,064
x0,378−0,064
=
0,040
x = 7,85 x 15 = 117,75 μg
t60 → 0,338 = 0,040 x + 0,064
x0,338−0,064
=
0,040
x = 6,85 x 15 = 102,75 μg
t90 → 0,214 = 0,040 x + 0,064
x0,214−0,064
=
0,040
x = 3,75 x 15 = 56,25 μg
t120 → 0,134 = 0,040 x + 0,064
x0,134−0,064
=
0,040
x = 1,75 x 15 = 26,25 μg

6. Perhitungan Faktor Koreksi


C15 = 109,5 μg = 0,109 mg
C30 2 mL
= 117,75 + ( x 109,5)
5 mL
=
117,75 + 14,6 = 132, 35 μg = 0,132 mg
C60 2 mL
= 102,75 + [ x (109,5+117,75)]
5 mL
=
102,75 + 30,3 = 133, 05 μg = 0,133 mg
C90 2 mL
= 56,25 + [ x (109,5+117,75+102,75)]
5 mL
=
56,25 + 44 = 100, 25 μg = 0,1 mg
C120 2 mL
= 26,25 + [ x (109,5+117,75+102,75+56,25)]
5 mL
=
26,25 + 51,5 = 77, 75 μg = 0,078 mg

7. Nilai Fluks (J)


Diketahui: D = 2 cm
Jt15 0,109 mg
= = 0,035 mg/cm2
3,14 cm²
Jt30 0,132mg
= = 0,0420 mg/cm2
3,14 cm²
Jt60 0,133 mg
= = 0,0424 mg/cm2
3,14 cm²
Jt90 0,1 mg
= = 0,032 mg/cm2
3,14 cm ²
Jt120 0,078 mg
= = 0,025 mg/cm2
3,14 cm²

8. Perhitungan % Difusi
Dosis = 2,5 mg
J tertinggi
% dosis = dosis x 100%
luas lingkaran
0,0424 mg/cm ²
= x 100%
2,5 mg/3,14 cm²
= 5,325 %

0.05

0.04

0.04

0.03

0.03

0.02

0.02

0.01

0.01

0
15 30 60 90 120

Grafik 1. Grafik Difusi Piroksikam (Waktu vs Nilai Fluks)

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