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Chlorella SP., Which Is A Green Microalga, Was Added For The Luminescence Experiment. The Seed

The document describes three separate experiments: 1. A microalgae cultivation experiment where Chlorella sp. was grown in a bioreactor under controlled conditions to monitor biomass concentration over time. 2. A PV cell fabrication experiment where different materials were deposited onto an ITO substrate to create a solar cell, which was then characterized and its power output calculated. A cooling system was implemented to regulate the cell's temperature. 3. A photosynthesis modeling experiment where a custom simulation was used to model photosynthesis based on an equation, ignoring photoinhibition effects.

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Rabiul Sikder
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38 views

Chlorella SP., Which Is A Green Microalga, Was Added For The Luminescence Experiment. The Seed

The document describes three separate experiments: 1. A microalgae cultivation experiment where Chlorella sp. was grown in a bioreactor under controlled conditions to monitor biomass concentration over time. 2. A PV cell fabrication experiment where different materials were deposited onto an ITO substrate to create a solar cell, which was then characterized and its power output calculated. A cooling system was implemented to regulate the cell's temperature. 3. A photosynthesis modeling experiment where a custom simulation was used to model photosynthesis based on an equation, ignoring photoinhibition effects.

Uploaded by

Rabiul Sikder
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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Materials and Methods:

Microalgae cultivation with spectral conversion material

Chlorella sp., which is a green microalga, was added for the luminescence experiment. The seed
culture was introduced to the bioreactor, which contained 200ml of BG-11 medium. The
illumination area was chosen to be 60 cm2. The initial dry cell weight (DCW) was measured and
was found to be 0.10-0.22 gL-1. Some specific conditions were maintained throughout the
experiment. The conditions were 250 rpm, a constant temperature of 35 0C, 0.6 suns of light
intensity, and 5 % (v/v) of CO2 and O2 supplementation. To monitor the concentration of
biomass, the optical density and dry cell weight was taken as standard and was constantly
measured. For the measurement of optical density, a spectrophotometer (DR 600, HACH) was
used. On the other hand, for the measurement of the dry cell weight, standard methods were
used.

PV fabrication and characterization

The device area was chosen to be approximately 0.15 cm2. Indium tin oxide was used for the
device material and the current density-voltage curve was obtained using the Lumerical
simulation software. The sequential approach was used for device fabrication by pouring MoO3,
DBP: C60, C60, and Ag layers onto a pristine substrate. The pristine substrate was made of ITO
and was put into a vacuum chamber. The maximum power was calculated using the MATLAB
and was found to be 30 Wm-2 at V = 0.75 Voltage under the illumination of 1 sun. A cooling fan
of 80 mm sq., 38 mm thick GA type was used to keep the surface of the PV cell cool. The fan
was controlled by the Arduino microcontroller. The controller was set so that the temperature of
the PV cell would not rise above 40 0C. The Arduino microcontroller was programmed using the
C programming language. An alarm was set with the microcontroller.

Modeling of Photosynthesis

The photosynthesis was modeled using the dual-energy generator, which is a custom-made
simulation.
The equation used in the photosynthesis model is as follows:

The wavelength (λ) was taken as the standard value. We have chosen the photosynthesis rate per
biomass to be 0.5 Wg-1. In the process of modeling photosynthesis, the photoinhibition effect
was ignored.

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