GloMax Discover

and Explorer
State-of-the-art multimode readers
for luminescence, fluorescence and
absorbance detection.
Easy-To-Use Integrated Assays Flexible
The intuitive touchscreen display, Optimized, preloaded Promega Select the detection modules you
preloaded protocols and automatic protocols are part of the GloMax® need. Place on a bench top or
instrument gain adjustments make Systems Software, minimizing time integrate into a larger automated
it simple to produce your data and spent optimizing instrument settings. platform for higher throughput.
analyze results.

OVERVIEW
VIDEO

Superior Performance World-Class Service

A broader dynamic range measures high and low samples Comprehensive standard warranty and our expert service
within the same experiment, resulting in better sensitivity team ready to answer questions about the instrument or
for low-level samples and lower well-to-well cross talk. chemistries. Additional service products, including Installation
and Operation Qualification (IQ/OQ) are available.
 The Right Instrument for Your Needs
GloMax Discover and Explorer offer a range of detection options.
®

Purchase the instrument and modules you need now. Upgrade your
system later as your laboratory needs change.

Visible UV-Visible BRET and

Model Luminescence Fluorescence
Absorbance Absorbance FRET
GloMax® Explorer
Model GM3510 ✔ ✔ Upgrade Upgrade Upgrade

GloMax® Explorer
Model GM3500 ✔ ✔ ✔ Upgrade Upgrade

GloMax® Discover
Model GM3000 ✔ ✔ ✔ ✔
Unpack, Plug In and
Start Generating Data
Automatic Instrument
Adjustments
No manual gain adjustment for luminescence reads.
Optimized settings are set automatically.

Reporting
Preloaded software provides many of the required
technical elements of a part 11 compliant system
when used with the appropriate laboratory workflow.

Minimal Manual Intervention

Easily multiplex assays with automated filter
switching, gaining more data from a single well.
Measure cell viability and gene reporter activity
with the touch of a button.

Data Analysis
Easily measure and analyze your results using the
intuitive, preloaded SMART Protocols software.
 Easy to Use for Your
Application
Automatic Filter Slides
• Multiple reads without user intervention
• Easily perform BRET and FRET studies
• Customize filters for your needs

Flexible Interface
• Select a preloaded protocol or customize your own
Integrated Filter Slides
• Set up a plate map
• View data in a heat map display
• Easily export your results to a variety of locations

Heat Map Display. Drag and Drop Protocols.

Integrated with Promega Assays
Superior performance for a wide array of assays for your research.
The sensitivity, broad dynamic range and low well-to-well cross talk
provide you with more usable and relevant data.

Developed and Optimized

at Promega
• 50 preloaded protocols
• Optimized settings produce data more quickly
• Protocols available for:
• Nucleic acid quantitation for next-generation
sequencing
• BRET and FRET for protein
interaction analysis
• Kinase and cell signaling assays
• Reporter gene assays
• Multiplexing assays
• Cell-based assays
• Bioassays
• ELISAs

Select your protocol of interest from the preloaded list.

Versatility and Performance

Multiplex Assays Screen Kinase Inhibitors

Easily read multiplexed cell viability, apoptosis and Efficiently read the output of kinase inhibitor screening
cytotoxicity assays. and automatically analyze results.
Percent Vehicle Control (Cytotoxicity)

Cytotoxicity 125
Percent Vehicle Control (Viability)

2,000 (CellTox™ Green) 150 EGFR

100 HER2

Percent Activity
1,500 HER4
100 75 IGF1R
Viability InsR
1,000
(CellTiter-Glo®) 50
KDR
50
500 PDGFRα
25
PDGFRβ
0 0 0

12143MA
–4 –3 –2 –1 0 1 2 3 4
11976MA

0.001 0.01 0.1 1 10 100 1,000

[digitonin], µg/ml Log10 [gefitinib], nM

Multiplexed cytotoxicity and viability assays performed on digitonin- Receptor tyrosine kinase inhibition was assessed following incubation with
treated K562 cells. gefitinib, a selective inhibitor of EGFR, using the ADP-GloTM Kinase Assay.
State-of-the-Art Sensitivity

Assess Reporter Bioassays Study Protein:Protein

Detect luminescence from Promega bioassays and Interactions
evaluate mAb activity. Analyze the interaction of proteins using BRET.

1 x 107 14
Luminescence (RLU)

12
8 x 106
10

mBRET Ratio
6 x 106
8
4 x 106 IC50 = 1.9µM
6

2 x 106 4

0 2
–11 –10 –9 –8 –7 –6 –5
0
11936MA

0.0001 0.001 0.01 0.1 1 10 100

12753MB
Log[Control Ab, Anti-CD20], g/ml
Nutlin-3 (µM)

ADCC Bioassay Target cells were incubated with a series of concentrations of A dose-response curve showing the disruption of the p53 and MDM2
Control Ab, Anti-CD20, followed by addition of ADCC Bioassay Effector Cells. interaction using the inhibitor Nutlin-3 in a NanoBRET™ assay.
Flexible
Workflow
Use on a bench top or integrate into
your automated workflow for higher
throughput and walk-away operation.

Automation Ready
Controlable by third-party scheduling
software with liquid handling and plate
stackers. The software is SiLA compat-
ible, making integration device control
simple as standards evolve.

Third Party Integration

Can be integrated with:
• Tecan Freedom EVO®
• Hudson Robotics Solo™
• PlateCraneEX™
• Hamilton Microlab® STAR series
• many other hardware systems
Single-Source
Service
and Support
Backed by a comprehensive one-year
standard warranty, and our expert
technical and service support teams.

One Call Supports It All

We supply the reagents and the
instrument, so our expert technical
support can answer all your questions.

Additional support ensures minimal

downtime when service is needed:
• Instrument loan program
• Service packages
• Extended warranty
Learn more about our extensive service
products at:
www.promega.com/GloMaxService
GloMax Systems Specifications
®

Luminescence
• Low-noise PMT ensures collected light is not compromised
• 10 to 1,000 times more sensitive than competitor instruments
• No need to dilute samples or manage gain settings
• 2 to 3 logs more usable data than competing instruments
• Filtered luminescence readout for BRET or multi-wavelength distinction (for GloMax® Discover only)
or total luminescence
• Lower cross talk for more usable data
• Five filters included for BRET and filtered luminescence assays such as NanoBRET™, Renilla/YFP BRET
and Chroma-Glo™ (for GloMax® Discover only)
• Or, customize all 5 luminescence filters to your needs (for GloMax® Discover only)

Luminescence Module Specifications

Detector Top-reading, head-on photon counting photomultiplier tube (PMT)

Wavelength Range 350–700nm
Detection Limit 3 × 10–21 moles of luciferase
Linear Dynamic Range 9 logs
Cross talk Less than 3 × 10–5 (white, 96-well plate, Corning 3912)
Filtered Luminescence GloMax® Discover: built-in filter paddle
Dynamic Range 9 logs
Sensitivity 3 × 10–21 moles of luciferase
GloMax Systems Specifications
®

Fluorescence
• Uses powerful wavelength-matched, light-emitting diodes (LED) as excitation sources
• LEDs closely match excitation profiles of commonly used fluorescent molecules to ensure high sensitivity
• Reduces nonspecific light leakage, a common problem when using broad-spectrum light sources
• Five excitation and emission filters included for common fluorescence assays; plus 1 customizable excitation and
emission position
• Or, customize two fluorescence excitation positions or all 6 emission positions to your needs

Fluorescence Module Specifications

Detector PIN-photodiode
Light Source Wavelength-matched LED
Read Position Top reading
Wavelengths selection Filter module with 5 standard excitation and emission filters. Empty filter
positions are available for custom excitation and emission wavelengths.
Wavelengths included UV (Ex: 365nm, Em: 415–445nm) Blue (Ex: 475nm, Em: 500–550nm)
Green (Ex: 525nm, Em: 580–640nm) Red (Ex: 625nm, Em: 660–720nm)
AFC (Ex: 405nm, Em: 495–505nm)
Detection Limit 2fmol fluorescein/200μl
Linear Dynamic Range >6 logs (assay dependent)
GloMax Systems Specifications
®

Absorbance
• GloMax® Discover comes factory installed with 9 UV-Visible filters for common colorimetric assays
• GloMax® Explorer GM3500 comes factory installed with 5 Visible Absorbance filters
• Ideal for ELISA, protein and nucleic acid assays

UV-Visible Absorbance Module Specifications

Detector Top-reading, head-on photon counting photomultiplier tube (PMT)
Light Source Xenon flash lamp
Spectra Range 200–600nm
Filter Wheel GloMax® Discover: 9 absorbance filters included
GloMax® Explorer: 5 absorbance filters
Wavelengths included GloMax® Discover: 230, 260, 280, 320, 405, 450, 490, 560 and 600nm 10nm
bandwidth
GloMax® Explorer: 405, 450, 490, 560 and 600nm 10nm bandwidth
Detection Limit 0.1 O.D.
Dynamic Range 0 – 4.0 O.D.
Ordering Information
Product Size Cat.#
GloMax® Discover System
1 each GM3000*
Configured with Luminescence, Fluorescence and UV-Visible Absorbance
GloMax® Explorer System
1 each GM3500*
Configured with Luminescence, Fluorescence and Absorbance
GloMax® Explorer System
1 each GM3510*
Configured with Luminescence and Fluorescence
*Comes with U.S. Instrument and Tablet PC power cords.

Accessories and Upgrades

Product Size Cat.#
GloMax® Dual Injectors with Pump Station 1 each GM3030
GloMax® Discover Luminescence Filter Paddle 1 each GM3011
GloMax® Discover Fluorescence Filter Paddle 1 each GM3012
GloMax® Discover Inner and Outer Injector Tubing, Natural 1 each GM3014
GloMax® Discover Inner and Outer Injector Tubing, Black 1 each GM3015
GloMax® Explorer Visible Absorbance Module Upgrade 1 each GM3520
GloMax® Explorer GM3500 Upgrade to GloMax® Discover GM3000 1 each GM3530
(only available for GM3500 model)
GloMax® Explorer GM3510 Upgrade to GloMax® Discover GM3000 1 each GM3540
(only available for GM3510 model)
Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.
Caspase-Glo, CellTiter-Glo, Dual-Glo, GloMax, Kinase-Glo, Nano-Glo, ONE-Glo and P450-Glo are registered trademarks of Promega Corporation. ADP-Glo, CellTox, Chroma-Glo, DLR and NanoBRET are trademarks of Promega Corporation.
Windows is a registered trademark of Microsoft Corporation.

To see the full line of GloMax® Instruments, visit www.promega.com/GloMaxSystems

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www.promega.com • © 2015 PROMEGA CORPORATION • ALL RIGHTS RESERVED • PRICES AND SPECIFICATIONS SUBJECT TO CHANGE WITHOUT PRIOR NOTICE • PUBLISHED IN USA 3/15 • 16342722 • PART BR261