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Practical of physiology
2nd stage
Practical of physiology
Leishman’s Stain
It is Romanosky group of stain consisting of:
*Eosin: It is an acidic dye and stains granules of eosinophils and hemoglobin of RBC.
*Acetone free methyl alcohol: It is fixes the smear to the glass slide.
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runs along the edge of the spreader. Then move the spreader slide to the left
direction.
Thick, viscous, blood should be spread rapidly with the spreader at a lower
angle. Thin, anaemic, blood should be spread slowly with the spreader held at a
greater angle (up to 50-60ᵒ).
Allow the smear to dry in air at room temperature; unheated table fans can be
used to hasten the process.
Stain the smear with Leishman's stain.
Staining of blood Smear
The slide is placed on stand/rod in horizontal position.
Leishman's stain (8-12 drops) is poured on slide so that it just covers the smear
fully.
It is kept for two minutes ,this is to fix the smear on the slide this is known as
(fixation time).
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Waiting time of (5-10) minutes is given. During this time the smear is actually
stained This is known as (staining time).
Proper staining procedure gives a green scum over the stain.
The slide is washed with tap water (taking care that water stream should not
strike smear directly).
The slide is kept vertically for drying.
Properly stained slide should look bluish pink.