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IAL Edexcel Biology Unit 3

This document contains frequently asked questions about working with a colorimeter, risks and safety precautions in the biology lab, evaluating the reliability and validity of experiments, controlling variables, calculating dry mass, measuring non-symmetrical areas, defining implications, and methods for improving studies. Safety topics covered include risks of burns, allergic reactions, and chemical exposure. Reliability is discussed in terms of sample size, number of experiments, and overlap of results. Validity considers use of controls and cross-checking sources.

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Willie Wong
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3K views

IAL Edexcel Biology Unit 3

This document contains frequently asked questions about working with a colorimeter, risks and safety precautions in the biology lab, evaluating the reliability and validity of experiments, controlling variables, calculating dry mass, measuring non-symmetrical areas, defining implications, and methods for improving studies. Safety topics covered include risks of burns, allergic reactions, and chemical exposure. Reliability is discussed in terms of sample size, number of experiments, and overlap of results. Validity considers use of controls and cross-checking sources.

Uploaded by

Willie Wong
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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IAL BIOLOGY UNIT 3

FREQUENTLY ASKED QUESTIONS


WORKING OF A COLORIMETER
Use a colorimeter to measure the colour intensity in arbitrary units.
Shake before measurement
Use of appropriate colour filter
Zeroing against blank
Shine light through the sample and measure the absorbance of light
RISK AND METHODS TO PREVENT IT
Hot water/scalding risk – wear a lab coat/ (heat resistant) gloves
(Bunsen) risk of burning – keep away from flame
Alkaline solution risk to skin or eyes – wear goggles or gloves/lab coat/wash
affected area immediately
Skin contact allergy – wear gloves/lab coat/wash affected area immediately/use
coated enzyme.
Inhalation allergy – wear mask/use coated enzyme
Spillages – clean up immediately
Ethanol is flammable – avoid contact with flame
Getting enzyme into blood through cut – wear plaster/gloves
CHECK IF AN EXPERIMENT IS RELIABLE
Check number of experiments performed for each independent variable, if large
amount, experiment is reliable.
Average found, reliable.
Sample size is large, reliable
No overlap
CHECK IF EXPERIMENT IS VALID
For section 1
Use of control hence valid
Comparing results, similar hence valid
No overlap hence valid
Standard deviation high, validity reduces.
For section 2
Check for any bias of sponsor
Check for credentials of contributor
Cross checking with another source
Evidence of peer reviewing
CONTROL OF VARIABLES
Temperature
Using water bath
Performing experiment inside a sealed room where temperature is constant
pH
Using a pH buffer
Light intensity
Idea of same light source e.g. light bulb
Idea of how same intensity/same distance
CONTROL OF VARIABLES
Humidity
Closed container/reduces evaporation
Carbon dioxide level
Cylinder/enclosed chamber
Wind speed
Cylinder/enclosed chamber
HOW TO CALCULATE DRY MASS
Remove water by placing in oven for sensible time, until constant mass
HOW TO MEASURE AREA OF NON-SYMMETRICAL
INHIBITION
Use graph paper, tracing diameter
Count squares
DIFFERENT IMPLICATION AND THEIR DEFINITION
Ethical implication
About the law e.g. ethics of keeping animals in zoo
Social implication
About the effects towards society e.g. educating the society
Environmental implication
Things that affect the environment both positive and negative
Economical implication
Money spent on projects
METHODS OF IMPROVING STUDY
Bigger sample size
Use of matched populations
Using people who have not come from failed drug treatment
Longer term study
More objective method of measurement of improvement needed
Double blind test stated
REFERENCE ORDER
1. Author
2. Year in brackets
3. Title
4. Journal
5. Volume
6. Issue in brackets
7. Page number

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