User Manual

Trinocular Infinity
Compound LED Microscope

Model M8333Z series

MicroscopeNet.com
 Table of Contents

i. Caution ....................................................................................................1

ii. Care and Maintenance.............................................................................2

1. Components Illustration ...........................................................................3

2. Installation ...............................................................................................5

3. Operation .................................................................................................7

4. Specifications ........................................................................................10

5. Optional Parts ........................................................................................11

6. Troubleshooting Guide ..........................................................................13

7. Darkfield Condenser Installation and Operation Instructions ..................15

8. Phase Contrast Kit Installation and Operation Instructions ....................17

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i. Caution
1. Open the carton carefully with a knife or paper cutter. Find the “UP” sign and place
the Styrofoam container on the side that makes the arrow upward. If the “UP” sign
is missing, please open the Styrofoam container gently to prevent any accessory
items (i.e. objectives or eyepieces) from dropping and being damaged.

2. Do not discard the molded Styrofoam container. The container should be retained
should the microscope ever requires reshipment.

3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty
environments. Ensure that the microscope is located on a smooth, level and firm
surface.

4. If any specimen solutions or other liquids splash onto the stage, objective or any
other component, disconnect the power cord immediately and wipe up the spillage.
Otherwise, the instrument may be damaged.

5. All electrical connectors (power cord) should be inserted into an electrical surge
suppressor to prevent damage due to voltage fluctuations.

6. For safety when replacing the LED light and the fuse, be sure the main switch is
off, unplug the power cord, and replace the LED light and the fuse.

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ii. Care and Maintenance

1. Do not attempt to disassemble any component including eyepieces, objectives or

focusing assembly.

2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on
metal surfaces should be cleaned with a damp cloth. More persistent dirt should be
removed using a mild soap solution. Do not use organic solvents for cleansing.

3. The outer surface of the optics should be inspected and cleaned periodically using
an air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth
or cotton swab dampened with a lens cleaning solution (available at camera stores).
All optical lenses should be swabbed using a circular motion. A small amount of
absorbent cotton wound on the end of a tapered stick makes a useful tool for
cleaning recessed optical surfaces. Avoid using an excessive amount of solvents as
this may cause problems with optical coatings or cemented optics or the flowing
solvent may pick up grease making cleaning more difficult. Oil immersion objectives
should be cleaned immediately after use by removing the oil with lens tissue or a
clean, soft cloth.

4. Observe the specimen with the 4X, 10X and 40X objectives in order, then observe
the specimen with the 100X objective. Apply the immersion oil on the slide cover with
the 100X objective. Do not let the immersion oil to contact with the dry objectives
lens (especially the 40X). Clean the dry objective lens using the lens cleaning paper
if the immersion oil is on the dry objectives lens. Clean the 100X objective lens first
using the lens cleaning paper after observing the specimen with the 100X objective,
then clean the specimen. More persistent dirt should be removed using a little bit
alcohol. Do not use organic solvents for cleansing.

5. Store the instrument in a cool, dry environment. Cover the microscope with the dust
cover when not in use.

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1. Components Illustration

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1

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2

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3
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4

15
5

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6

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7 7

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7

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8
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20
23
3
24
21

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1 Eyepiece 11 Trinocular Viewing Head 21 Stage Upward Stopper

2 Diopter Ring 12 Swapping Lever 22 Condenser
3 Eyepiece Tube 13 Head Lock Thumb Screw 23 Aperture Diaphragm Ring
4 Nosepiece 14 Objectives 24 Condenser Centering Screws
5 Slide Holder 15 Microscope Body 25 Filter
6 Mechanical Stage 16 Brightness Intensity Dial
7 X-Y Stage Moving Knobs 17 Focus Tension Ring
8 Light Collector 18 Coarse Focus Knob
9 Microscope Base 19 Fine Focus Knob
10 Photo Tube 20 Condenser Focus Knob

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2. Installation
2.1 Installation of the trinocular viewing head
1) Loosen the head lock thumb screw on the top of the microscope body and remove
the plastic cover on the top.
2) Remove the cap on the dovetail of the trinocular viewing head.
3) Seat the dovetail of the viewing head (Fig. 1 (a)) into the socket on the top of the
microscope body (Fig. 1 (b)).
4) Rotate the viewing head as shown in Fig. 1 (c).
5) Tighten the head lock thumb screw (Fig. 1(d)).
Caution:
Do not release the viewing head until you are sure the viewing head is installed
securely.

Head Lock
Thumb Screw

(a)

(b) (c) (d)

Fig.1
2.2 Installation of the photo tube
1) Remove the cap on the top of the viewing head.
2) Thread the photo tube into the top of the viewing head.
2.3 Installation of the eyepieces
3) Remove the protective caps from the eyepiece tubes.
4) Insert the eyepieces into the eyepiece tubes.
2.4 Installation of the objectives
1) Adjust the coarse focus knob until the mechanical stage is at its lowest position.
2) Turn the caps counter-clockwise to remove them from the nosepiece.
3) Take the objectives out from the plastic cases and turn each one clock-wise into the
holes on the nosepiece. Install the 4X objective into the nosepiece first. Then in a

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counter-clockwise direction, rotate the nosepiece and install each succeeding higher
magnification objective as shown in Fig. 2.

Note: Fig.2
 Inspect the objectives frequently for dirt or oil; clean if necessary.
 Use the 10X objective to initially focus the image of your specimen.
 When changing the objective magnification, rotate the objective nosepiece until you
hear a “click” sound or have a clear "in position" feeling. This ensures the objective
is centered in the optical light path.
2.5 Installation of the color filters
The color filter is simply put into the filter holder
2.6 Connecting the power cord
1) Turn the power switch to the off position.
2) Connect the power cord to the socket at the back of microscope body and plug the
other end into a wall outlet.
Caution:
Before connect the cord to the wall outlet; make sure the voltage switch is slide to
the correct position for the right power source.
2.7 Replacing the fuse
Fuse
1) Turn off the power switch and disconnect the power
cord.
2) Find the fuse holder at the back of the microscope
body.
3) With a flat-head screwdriver, press and turn the
fuse holder counter clock wise to remove it.
4) Replace the old fuse with a new one,
5) Put the fuse holder back, press and turn it clock Fig.3
wise. See Fig. 3.

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3. Operation
3.1 Adjusting illumination
1) Plug the power cord into the power socket on the microscope and connect it to the
power outlet.
2) Turn on the power switch.
3) Rotate the brightness intensity dial to increase or decrease the brightness of the
illuminator.
3.2 Placing specimen
1) Place the slide on the mechanical stage.
2) Use the slide holder to gently secure the slide.
3) Turn the X and Y stage moving knobs to position the specimen in the center of
viewing field.
Caution:
Be sure not to allow an objective to touch a
specimen slide when changing objectives. Condenser Focus Knob

3.3 Focusing
1) With the 10X objective in position, raise the
mechanical stage using the coarse focus
knob until the specimen is close to the
objective.
2) Turn the coarse focus knob until the
specimen is in focus.
3) Use the fine focus knob to obtain a sharp
image. Condenser Centering Screws
4) Turn the condenser focus knob (Fig. 4) to
Fig.4
raise or lower the condenser till the image
of field is focused.
5) Turn the condenser centering screws (Fig. 4) to move the image of the field into the
center of the viewing field.
6) To get a good focused image, you may need to combine the focus knob adjustment
and interpupillary distance adjustment, along with eyepiece diopter adjustment
stated in 3.4 and 3.5.
7) You may now switch to another magnification objective.
Tips:
a) The condenser is raised when using high
power objectives and lowered when using low
power objectives.
b) To prevent your specimen slide from making
contact with an objective, raise the stage to its
highest position without contacting the 100X
objective; then tighten the stage upward
stopper (Fig. 5). Give the stage a tiny extra
moving space to ensure the objective can be
focused every time. Stage Upward Stopper

Fig.5

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3.4 Adjusting interpupillary distance
While observing with both eyes, hold the left and right eyepiece tubes then swing
them around the center axis. The interpupillary distance is correct when the left and
right fields of view converge completely into one image.
3.5 Adjusting eyepiece diopter
1) Using the 10X objective and your right eye only, observe your specimen through the
eyepiece and bring it into focus by adjusting the focus knobs.
2) Then observe the specimen with your left eye only through the left eyepiece. If the
specimen is not in focus, turn the diopter ring on the eyepiece tube until a sharp
image is obtained.
3.6 Applying the immersion oil
1) Rotate the objective nosepiece to seat the observing position between the 40X and
100X objectives as shown in Fig. 6 (a).
2) Place a drop of immersion oil on the slide cover as shown in Fig. 6 (b).
3) Rotate the objective nosepiece to seat the 100X objective to the observing position
until you hear a “click” sound.
4) After observing the specimen, use the lens cleaning paper to clean the 100X
objective lens gently and the specimen in time.
5) If it is hard to clean, you need a little bit alcohol to clean the 100X objective lens and
the specimen.

Caution (important): (a) Fig.6 (b)

 When you use the 100X objective to observe the specimen, you have to finish
observing the specimen with the 4X, 10X, 40X objectives.
 When you use the 100X objective to observe the specimen, you have to apply the
immersion oil on the top of the slide cover.
 When you apply the immersion oil with the 100X objective, do not let the immersion
oil to contact with the dry objective lenses (especially the 40X). If the immersion oil
is on the dry objectives lens, please use the lens cleaning paper to clean the
objectives lens in time. The oil will damage the dry objective lenses.
 After observing the specimen with the 100X objective, clean the 100X objective lens
first.
3.7 Adjusting iris aperture diaphragm
Turn the aperture diaphragm ring to adjust the aperture size.

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3.8 Adjusting focus knob tension
The tightness of the focus knob tension has been pre-set at the factory. If the
mechanical stage drops by itself, rotate the focus tension ring (Fig. 7) with the
tension wrench situated between the coarse focus knob and microscope body until
the tension is in maintained.
Focus Tension Ring

Camera

Fig.7 Fig.8

3.9 Photo/video observing, capturing and recording

1) Insert camera into the photo tube (Fig. 8).
2) Bring the microscope into focus by following the
procedures in 3.3.
3) Pull the swapping lever out (Fig. 9)
4) Adjust the photo tube length until image on camera is in
focus.

Note:
 The swapping lever to switch beam spilt for photo part and
100% to eyepieces when photo tube is not in use.

Fig.9

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4. Specifications
Model M8333Z
Total Magnification 40X, 100X, 250X, 400X, 1000X, 2500X

1 pair of WF10X/18
Eyepieces
1 pair of WF25X

Infinity objectives achromatic

4X/0.10 ∞/-
Objectives 10X/0.25 ∞/0.17
40X/0.65 ∞/0.17 (spring)
100X/1.25 ∞/0.17 (spring, oil)

30ºinclined, 360ºswiveling siedentopf trinocular viewing head

Hinge interpupillary distance adjustment, 2-3/16” ~ 2-5/16”
(55mm ~ 75mm)
Viewing Head
Diopter adjustment on left eyepiece tube
A lever to switch between beam spilt for photo part and 100% to
eyepieces when camera is not in use

Nosepiece Reversed revolving quadruple nosepiece

Double layer mechanical stage

Stage Dimension: 5-1/2” x 4-1/4” (140mm x 110mm)
Translation range: 3” x 1-13/16” (78mm x 46mm)

NA=1.25, built-in aperture iris diaphragm

Condenser
Center adjustable, rack and pinion adjustment

Coaxial coarse and fine focusing knobs on both sides with focus
Focus Mechanism
stop and tension control

Collector With color filter holder

Color filter 45mm green filter in diameter

Illumination Transmitted, 3W LED, intensity adjustable

Power Supply AC 100-240V (US and Canada plug)

Cameras (optional) Refer to the cameras specifications

Darkfield Condensers (optional) Refer to the darkfield condensers specifications

Phase Contrast Kit (optional) Refer to the phase contrast kit specifications

Dimension 19-3/4” x 16” x 8” (50 cm x 41 cm x 20 cm)

Net weight 17 lbs (7.7 kg)

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5. Optional Parts
(The optional parts may be included in some models or sold separately.)
1) Cameras
Model Sensor Resolution Operating System Software

A35100U 3584 x 2748 (10.MP)

CMOS
USB2.0
A35140U 4096 x 3288 (14.0MP)

A3550U3 2560 x 1922 (5.0MP)

MS Windows
A3580U3 3328 x 2548 (8.0MP) Mac OS 10.8 and up Included
Linux (2.6 or above)
CMOS
A35100U3 3584 x 2746 (10.0MP)
USB3.0

A35140U3 4096 x 3286 (14.0MP)

A35180U3 4912 x 3684 (18.0MP)

CPZJ-150R CMOS 2560 x 1920 (5.0MP) N/A N/A

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2) Darkfield Condensers
Darkfield Numerical Mounting
Model Objective
Condenser Aperture Size(diameter)

A191-INF Dry 0.7-0.9 - 37mm

A191BD-
Oil 1.36-1.25 - 37mm
INF

3) Phase Contrast Kits

Centering
Model Phase Contrast Objective Condenser Annular Ring Plates
Telescope
Plan achromatic 10X with Five positions:
built-in phase plate 10 for 10X phase contrast
objective
Plan achromatic 20X with
20 for 20X phase contrast
built-in phase plate
objective
40 for 40X phase contrast Focusing
A1PHIP Plan achromatic 40X with NA 1.25
built-in phase plate, spring objective adjustable
100 for 100X phase
Plan achromatic 100X with contrast objective
built-in phase plate, spring, B for bright field
oil observation, with iris
diaphragm

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6. Troubleshooting Guide
Problem Cause Solution

No electrical power Check power cord connection

Lamp does not
light when LED or power unit dead Replace LED light
switched on
Fuse blown out Replace fuse

Revolve the nosepiece to click-stop

Revolving nosepiece not in click stop
position by swinging the objective
position
Darkness at the correctly into the optical path
periphery or
uneven brightness The field iris diaphragm are not Open field diaphragm
in the field of view opened enough

The field diaphragm not in the center Center the field iris diaphragm

Dirt or dust on the eyepiece,

Dirt or dust on the
condenser, objective, collector lens Clean the lens with a lens cleaning paper
view
or specimen

No slide cover attached to the slide Attach a 0.17mm slide cover

Use a slide cover of the appropriate

Slide cover is too thick or thin
thickness (0.17mm)

Slide may be upside down Turn slide over so the cover-glass faces
(specimen at the bottom) up

Diopter adjustment is not set

Readjust the diopter settings
properly

Immersion oil is on a dry objective

Check the objectives, clean if necessary
(especially the 40X)
Poor image
quality or not able
No immersion oil used with 100X
to get focused Use immersion oil
objective
image
Air bubbles in immersion oil Remove bubbles

Aperture is closed or open too much Open or close properly

Condenser is not in the right position Adjust the condenser

The field diaphragm not in the Center the field iris diaphragm
center

The aperture and field iris diaphragm Open and adjust the aperture and field
are not opened enough diaphragm

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Specimen rises from stage surface Secure the specimen in the slide holder

Revolving nosepiece is not in the Revolve the nosepiece to the click-stop

click-stop position position

Adjust the light intensity by rotating the

Lamp intensity is too high or low
intensity control dial

Slippage of focus Increase the tension on the focusing

Tension adjustment is set too low
when using the knobs
coarse focusing
knob fine focus is
Tension adjustment is set too high Loosen the tension on the focusing knobs
ineffective

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7. Darkfield Condenser Installation and Operation

Instructions
7.1 Dry darkfield condenser of A191-INF
1) Mounting the dry darkfield condenser
 Rotate the nosepiece and set the 4X objective in position.
 Turn the coarse focus knob to raise the mechanical stage to the highest position
without contacting the 4X objective.
 Turn the condenser control knob to lower the condenser to the lowest position.
 Loosen the condenser lock thumb screw on the condenser holder and remove the
brightfield condenser as shown in Fig. 10 (a).
 Install the dry darkfield condenser and tighten the condenser lock thumb screw on
the condenser holder as shown in Fig. 10 (c).
 Re-adjust the condenser height to its normal position.
 Re-adjust the mechanical stage height to its normal position.
Condenser Holder Condenser Lock Thumb Screw

(a) Condenser Centering Screws (b) (c)

Fig.10
2) Centering the dry darkfield condenser
 Turn the 4X objective to the light path.
 Turn the condenser focus knob to lower the
condenser till a dark spot showed in the viewing field
as shown in Fig. 11 (a).
 Turn the condenser translational centering screws to
move the dark spot to the center as shown in Fig. 11 (b). (a) (b)
3) Place the slide on the stage. Fig.11
4) Raise the condenser all the way to the top and lower it a little bit. (a) (b)
5) Following the procedures in this manual to focus and observe.
6) Move the condenser up or down slightly to get the best darkfield viewing.

Note：
 The dry darkfield condenser is used with the dry objectives only.
 The dry darkfield condenser works with the 4X, 10X, 40X objectives.
 The dry darkfield condenser won’t work with the 100X oil immersion objective.

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7.2 Oil darkfield condenser of A191BD-INF
1) Mounting the oil darkfield condenser
 Rotate the nosepiece and set the 4X objective in position.
 Turn the coarse focus knob to raise the mechanical stage to the highest position
without contacting the 4X objective.
 Turn the condenser control knob to lower the condenser to the lowest position.
 Loosen the condenser lock thumb screw on the condenser holder and remove the
brightfield condenser as shown in Fig. 12 (a).
 Install the oil darkfield condenser and tighten the condenser lock thumb screw on
the condenser holder as shown in Fig. 12 (c).
 Re-adjust the condenser height to its normal position.
 Re-adjust the mechanical stage height to its normal position.
Condenser Holder Condenser Lock Thumb Screw

(a) Condenser Centering Screws (b) (c)

Fig.12
2) Centering the oil darkfield condenser
 Turn the 40X objective to the light path.
 Turn the condenser focus knob to slowly lower and
raise the condenser till a dark spot showed in the
viewing field as shown in Fig. 13 (a).
 Turn the condenser translational centering screws to
(a) (b)
move the dark spot to the center as shown in Fig. 13 (b).
Fig.13
3) When using the 40X objective to observe the specimen.
 Place the slide on the stage.
 Lower the condenser until a sharp brightfield image showing in the viewing field.
 Raise the condenser until the darkfield image showing in the viewing field.
4) When using the 100X objective to observe the specimen
 Raise the condenser until the top is close to the opening of stage.
 Place a drop of immersion oil on the top of condenser.
 Place the slide on the stage.
5) Raise the condenser and let the oil drop contact the bottom of the slide. If air
bubbles exist in the oil, clean the oil from the top of the condenser and bottom of
slide with a lens cleaning paper and repeat the procedures.
6) Follow the procedures in this manual to bring the sample on slide in focus and
observe.
7) After observing the specimen with 100X objective, use the lens cleaning paper to

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gently clean the 100X objective lens, oil darkfield condenser and the specimen in
time.
8) If it is hard to clean, you need a little bit alcohol to clean the 100X objective lens, oil
darkfield condenser and the specimen.
Note：
 When using the 40X objective, you won’t need to apply oil drop on the oil condenser.
 When using the 100X objective, the condenser won’t work well if no oil drop applied
on the condenser.
 When you apply the immersion oil with the 100X objective, do not let the immersion
oil to contact with the dry objective lenses (especially the 40X). If the immersion oil
is on the dry objectives lens, please use the lens cleaning paper to clean the
objectives lens in time. The oil will damage the dry objective lenses.
 After observing the specimen with the 100X objective, clean the 100X objective lens
first.

8. Phase Contrast Kit Installation and Operation

Instructions
8.1 Phase contrast kit of A1PHIP:
1) Mounting the phase contrast objectives
a. Take off all the objectives from the nosepiece.
b. Install the phase contrast objectives onto the nosepiece following the steps in 2.4.
2) Mounting the phase contrast condenser/annular ring disk
a. Rotate the nosepiece and set the 10X objective in position.
b. Turn the coarse focus knob to raise the mechanical stage to the highest position
without contacting the 10X objective.
c. Turn the condenser control knob to lower the condenser to the lowest position.
d. Loosen the condenser lock thumb screw and remove the brightfield condenser as
shown in Fig. 14 (a).
e. Insert the condenser/annular ring disk into the condenser holder, and tighten the
thumb screw as shown in Fig. 14 (c).
f. Re-adjust the condenser height to its normal position.
g. Re-adjust the mechanical stage height to its normal position.
Condenser Holder Condenser Lock Thumb Screw

(a) Condenser Centering Screws (b) (c)

Fig.14
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Note:
 When raising the mechanical stage, do not make 40
contact with the objective.
3) Turn the desired objective into light path.
4) Turn the annular ring disk to put the corresponding
ring into light path, i.e. if you are using the 40X
phase contrast objective, you should turn the disk
at 40 as shown in Fig. 15.
5) Centering the annular ring. Fig.15
a. Remove one eyepiece from the microscope eyepiece tube and
insert the centering telescope as shown in Fig. 16.
b. Observe from the telescope. The bright ring and dark ring
should be coincided with each other as shown in Fig.17 (d).
c. If the ring images are not clear, turn the top of telescope
until both ring images are in focus.
d. If the bright ring is still obscure as in Fig.17 (b), adjust
the condenser focus knob.
Fig.16
e. If the two ring images are not coincided as shown in
Fig.17 (c):
 Turn the condenser centering screws (Fig. 18) to
move the dark spot to the center until two ring
images are coincided, or
 Turn the adjustment knobs (Fig. 18) to move the (a) (b)
dark spot to the center until two ring images are
coincided.
f. Remove the centering telescope and replace it with
the eyepiece.
g. Put the specimen on the stage and adjust the
illumination, focusing, etc following the instructions in
this manual. (c) (d)
Note: Fig.17
 The phase contrast condenser will be working as a
conventional Abbe condenser if the annular ring Adjustment Knobs
disk being put at BF position.
 The phase contrast condenser will be working as a
dry darkfield condenser if the annular ring disk
being put at DF position.
6) Performing the phase contrast observation.
After centering the condenser ring plate, perform
the phase contrast observation the same way as a normal Condenser Centering Screws
bright field microscope.
Note: Fig.18
 When change to another phase contrast objective and corresponding condenser
ring plate, the focusing and centering of bright ring and dark ring should be repeated
following the procedures from 5)-b to 5)-f.

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