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Unisense Microsensors in Biofilm Research

The document discusses microsensor solutions for making microscale measurements in biofilms. It describes how Unisense microsensors can be used to monitor various analytes like oxygen, hydrogen sulfide, and pH in biofilms in real-time and with μm scale spatial resolution. The MicroProfiling System allows precise positioning of sensor tips smaller than 10 μm directly in biofilms to obtain microprofiles and maps of analyte production and consumption. The system can profile biofilms manually or automatically in 2D and 3D. Field systems are also available to monitor biofilms in remote environments. Examples are given of oxygen profiling through algal biofilms and into P. aeruginosa colonies.

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38 views2 pages

Unisense Microsensors in Biofilm Research

The document discusses microsensor solutions for making microscale measurements in biofilms. It describes how Unisense microsensors can be used to monitor various analytes like oxygen, hydrogen sulfide, and pH in biofilms in real-time and with μm scale spatial resolution. The MicroProfiling System allows precise positioning of sensor tips smaller than 10 μm directly in biofilms to obtain microprofiles and maps of analyte production and consumption. The system can profile biofilms manually or automatically in 2D and 3D. Field systems are also available to monitor biofilms in remote environments. Examples are given of oxygen profiling through algal biofilms and into P. aeruginosa colonies.

Uploaded by

Amin
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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SOLUTIONS FOR MICROSC ALE MEASUREMENTS

ENABLING

SCIENTIFIC

RESEARCH

Unisense microsensors in biofilm research


Real time monitoring of H2S, O2, NO, pH, Redox, H2, N2O or temperature in your biofilm

•• Real time and true µm scale measurements •• Complete systems from sensors to software
•• In laboratory and in the field •• Study production and consumption of all analytes
•• Non-destructive measurements •• Interdisciplinary – biomedical, environmental and industrial

Take advantage of sensor tips with diameters less than 10 µm and complete real time measurements directly in your biofilm. Regardless of
whether you measure oxygen availability in nitration biofilms, N2O production in sputum, or maybe photosynthetic activity of microalgal
biofilms, the Unisense microsensor technology will provide you with an accurate and reliable research tool.

Using the MicroProfiling System you can complete microprofiles with extreme positioning accuracy and high spatial resolution. The micropro-
files can be performed manually operating the micromanipulator by hand, or automatically controlling a motorstage via your PC. The sensor
signal is logged using SensorTrace Suite software, a software solution that also allows you to visualize and analyse your obtained data. The
MicroProfiling System can be augmented with one or two more degrees of freedom, enabling you to conduct measurements in both two and
three dimensions.

For research that requires measurement conducted in the field, we can also provide a solution that will allow you to conduct your research at
remote locations without the need of human interaction once the equipment has been placed. The Field MicroProfiling System together with
auxiliary equipment will allow the precise placement of the sensors and automated profiling. With this setup you can obtain a spatial resolu-
tion and accuracy of 10µm in an in situ measurement.

Unisense MicroProfiling System


SOLUTIONS FOR MICROSC ALE MEASUREMENTS

Selected microsensor applications:

A B

C D

A & B) Oxygen profile down through algae biofilm and oxygen measure setup displaying the MicroProfiling System.
Courtesy of Dr. R. Gardner and Dr. H.C. Bernstein, Montana State University and Pacific Northwest National Laboratory.
C & D) Unisense 10µm sensor profiling into a P. Aeruginosa colony and associated oxygen profiles.
Courtesy of Dr. L. Dietrich, Columbia University.

Selected papers in which Unisense microsensors are used for biofilm research:
Bernstein, H.C., Kesaano, M., Moll, K., Smith, T., Gerlach, R., Carlson, R.P., Miller, C.D., Peyton, B.M., Cooksey, K.E., Gardner, R.D., Sims, R.C.,
Direct measurement and characterization of active photosynthesis zones inside wastewater remediating and biofuel producing
microalgal biofilms, Bioresource Technology 156, 206–215 (2014).

Morales D.K., Grahl N., Okegbe C., Dietrich L.E.P., Jacobs N.J., Hogan D.A., Control of Candida albicans metabolism and biofilm forma-
tion by Pseudomonas aeruginosa phenazines, mBio 4(1):e00526-12 (2013).

Kempes C., Okegbe C., Mears-Clarke Z., Follows M., Dietrich L.E.P., Morphological optimization for access to dual oxidants in biofilms,
Proceedings of the National Academy of Sciences of the United States of America 111(1):208-13 (2014).

Shanahan, J.W., Semmens, M.J., Influence of a nitrifying biofilm on local oxygen fluxes across a micro-porous flat sheet membrane,
Journal of Membrane Science, Volume 277, Issues 1–2, 1 June, Pages 65–74 (2006).

Kolpen M., Kühl M., Bjarnsholt T., Moser C., Hansen C.R., Nitrous Oxide Production in Sputum from Cystic Fibrosis Patients with
Chronic Pseudomonas aeruginosa Lung Infection. PLoS ONE 9(1): e84353 (2014).

Behrendt L., Larkum A.W., Trampe E., Norman A., Sørensen S.J., Kühl M., Microbial diversity of biofilm communities in microniches as-
sociated with the didemnid ascidian Lissoclinum patella, The ISME Journal 6, 1222–1237 (2012).

Unisense A/S - Tueager 1, DK-8200 Aarhus N, Denmark - www.unisense.com

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