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Name: - Reg. No.: - Dissolved Oxygen Measurement

This document describes a procedure for determining dissolved oxygen (DO) levels in water samples using the modified Winkler (azide modification) method. Key steps include collecting aerated water samples in BOD bottles, adding manganese sulfate and alkali-iodide-azide reagents to form a precipitate if oxygen is present, acidifying the sample to dissolve the precipitate, titrating the sample with sodium thiosulfate solution while adding starch indicator until the blue color disappears, and calculating the DO level in mg/L based on the titrant volume used. The test relies on DO in the sample oxidizing manganese ions which are then reduced back during titration, allowing for precise and reliable DO measurements in natural and wast

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Eshwar Nukala
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77 views

Name: - Reg. No.: - Dissolved Oxygen Measurement

This document describes a procedure for determining dissolved oxygen (DO) levels in water samples using the modified Winkler (azide modification) method. Key steps include collecting aerated water samples in BOD bottles, adding manganese sulfate and alkali-iodide-azide reagents to form a precipitate if oxygen is present, acidifying the sample to dissolve the precipitate, titrating the sample with sodium thiosulfate solution while adding starch indicator until the blue color disappears, and calculating the DO level in mg/L based on the titrant volume used. The test relies on DO in the sample oxidizing manganese ions which are then reduced back during titration, allowing for precise and reliable DO measurements in natural and wast

Uploaded by

Eshwar Nukala
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Name: __________________ Reg. No.

:____________
Dissolved Oxygen measurement
Experiment: Determination of Dissolved Oxygen (DO) levels aerated water sample(s) by modified
Winkler (Azide Modification) method.

Apparatus required: 300 mL BOD bottles, conical flasks, burettes, pipettes, measuring cylinders.

Reagents required: Manganous sulfate solution, alkali-iodide-azide reagent, standard sodium thiosulfate
titrant, starch solution and conc. sulphuric acid.

Theory:
Dissolved oxygen (DO) levels in natural and wastewaters depend on the physical, chemical and biological
activities in the water body. The replenishment of DO through natural or artificial means is important.
The winkler test is the most precise and reliable titrimetric procedure for DO analysis. It is based on the
addition of divalent manganese solution, followed by strong alkali, to the sample in a glass stoppered
bottle. DO present in the sample rapidly oxidizes an equivalent amount of the dispersed divalent
manganous hydroxide precipitate to hydroxides of higher valency states. In the presence of iodide ions in
an acidic solution, the oxidized manganese reverts to the divalent state, with the liberation of iodine
equivalent to the original DO content. The iodine is then titrated with a standard solution of thiosulphate.

Procedure:
1. Collect aerated water samples in 300-mL BOD bottles at regular intervals from the aspirator bottle
(which serves as the storage of water sample under constant aeration).
2. To the sample collected in 300-mL bottle from the aspirator bottle, add 1 mL MnSO4, followed by 1mL
alkali-iodide-azide reagent. Reddish brown precipitate will be formed if DO is present in water.
3. Allow the precipitate to settle sufficiently (to approximately half the bottle volume) to leave clear
supernate above the manganese hydroxide.
4. Add 1 mL conc. H2SO4. Mix by inverting several times until dissolution is complete.
5. Take 201 mL sample which will serve as effective 200 mL sample in a conical flask. Take the initial
burette reading.
6. Titrate with 0.025 M Sodium thiosulphate solution to a pale straw colour.

1
7. Add a few drops of starch solution and continue titration to first disappearance of blue colour. Record
the final reading. The difference between final and initial burette reading will give the amount of sodium
thiosulphate solution consumed during the titration.

Record your observations:


1 mL of 0.025 M Na2S203 = 1 mg DO/L

Sl No. Bottle No. Burette reading, mL DO


(mg/L)
Initial Final Difference

Further Reading:

1. Chapter 4500 dealing with Dissolved Oxygen from Standard Methods for Examination of Water and
Wastewater by APHA, AWWA & WEF from Water Environment Federation, USA
2. Chapter 21 dealing with Dissolved Oxygen from Chemistry for Environmental Engineering and
Science by Clair Sawyer, Perry McCarty & Gene Parkin from Tata McGraw Hill India.

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