MAGNESIUM

COD 11512 4 x 50 mL

STORE AT 2-8ºC
Reagents for measurement of magnesium concentration MAGNESIUM
Only for in vitro use in the clinical laboratory
CALMAGITE

PRINCIPLE OF THE METHOD − Repeatibility (within run):

Magnesium in the sample reacts with calmagite in alkaline medium forming a coloured complex Mean Concentration CV n
that can be measured by spectrophotometry. EGTA is included in the reagent to remove calcium 1.7 mg/dL = 0.70 mmol/L 1.1 % 20
interference1,2. 3.9 mg/dL = 1.60 mmol/L 1.8 % 20

CONTENTS AND COMPOSITION − Reproducibility (run to run):

A. Reagent. 4 x 50 mL. Calmagite 80 µmol/L, EGTA 60 µmol/L, diethylamine 0.2 mol/L. Mean Concentration CV n
S. Calcium/Magnesium Standard. 1 x 5 mL. Calcium 10 mg/dL, magnesium 2 mg/dL (0.82 1.7 mg/dL = 0.70 mmol/L 3.0 % 25
mmol/L). Aqueous primary standard. 3.9 mg/dL = 1.60 mmol/L 2.2 % 25

STORAGE − Sensitivity: 64 mA⋅dL/mg = 26.24 mA⋅L/mmol.

Store at 2-8ºC. − Trueness: Results obtained with this reagent did not show systematic differences when
Reagent and Standard are stable until the expiry date shown on the label when stored tightly compared with reference reagents (Note 3). Details of the comparison experiments are
closed and if contaminations of the reagent are prevented during their use. available on request.
Indications of deterioration: − Interferences: bilirubin (20 mg/dL) does not interfere. Hemolysis and lipemia interfere. Other
drugs and substances may interfere4.
− Reagent: Presence of particulate material, turbidity, absorbance over 0.575 at 520 nm (1 cm
cuvette). These metrological characteristics have been obtained using an analyzer. Results may vary if a
different instrument or a manual procedure is used.
− Standard: Presence of particulate material, turbidity.
DIAGNOSTIC CHARACTERISTICS
REAGENT PREPARATION
Magnesium is one of the most abundant cations in the body. It is stored mainly in bone but
Reagent and Standard are provided ready to use. significant amounts are contained in gastric and biliary secretions. Magnesium acts as an
essential cofactor for enzymes concerned with cell respiration, glycolisis, and transmembrane
ADDITIONAL EQUIPMENT transport of other cations.
− Analyzer, spectrophotometer or photometer with cell holder able to read at 520 ± 20 nm. Plasma magnesium concentration is normally kept within narrow limits. The kidneys are the main
organs of magnesium homeostasis in maintaining plasma concentrations.
SAMPLES
Increased serum magnesium concentrations have been observed in dehydration, severe diabetic
Serum or plasma collected by standard procedures. Hemolysed and lipemic samples are not
acidosis, Addison’s disease, and conditions that interfere with glomerular filtration3,5.
suitable for testing.
Low magnesium concentration in plasma is found as a result of gastrointestinal malabsorption,
Magnesium in serum or plasma is stable for 10 days at 2-8ºC. Use heparin as anticoagulant.
fluid losses, renal losses caused by diuretic therapy and aminoglucoside therapy. It also may be
PROCEDURE due to hypoparathyroidsm and alcoholism3,5.
Clinical dianosis should not be made on the findings of a single test result, but should integrate
1. Bring the Reagent to room temperature.
both clinical and laboratory data.
2. Pipette into labelled test tubes: (Notes 1, 2)
NOTES
Blank Standard Sample
1. Contamination of glassware with magnesium will affect the test. Use acid-washed glassware
Magnesium Standard (S)  10 µL  or plastic tubes.
Sample   10 µL
2. The reagent may be used in several automated analyzers. Instructions for many of them are
Reagent (A) 1.0 mL 1.0 mL 1.0 mL
available on request.
3. Mix thoroughly and let stand the tubes for 2 minutes at room temperature. 3. Calibration with the provided aqueous standard may cause a matrix related bias, specially in
4. Read the absorbance (A) of the Standard and the Sample at 520 nm against the Blank. The some analyzers. In these cases, it is recommended to calibrate using a serum based standard
colour is stable for at least 1 hour. (Biochemistry Calibrator, cod. 18011 and 18044).

CALCULATIONS BIBLIOGRAPHY
The magnesium concentration in the sample is calculated using the following general formula: 1. Chauman UPS and Ray Sarkar BC. Use of calmagite for the determination of traces of
magnesium in biological materials. Anal Biochem 1969; 32: 70-80.
A Sample
x C Standard = C Sample 2. Gindler EM and Heth RA. Colorimetric determination with bound “calmagite” of magnesium in
A Standard human blood serum. Clin Chem 1971; 17: 662.
If the Magnesium Standard provided has been used to calibrate (Note 2): 3. Tietz Textbook of Clinical Chemistry, 2nd edition. Burtis CA, Ashwood ER. WB Saunders Co.,
1994
A Sample x 2 = mg/dL magnesium 4. Young DS. Effects of drugs on clinical laboratory tests, 3th ed. AACC Press, 1997.
A Standard x 0.82 = mmol/L magnesium 5. Friedman and Young. Effects of disease on clinical laboratory tests, 3th ed. AACC Press,
1997.
REFERENCE VALUES
Serum and plasma3: 1,7-2,4 mg/dL = 0,70-0,98 mmol/L.
These ranges are given for orientation only; each laboratory should establish its own reference
ranges.

QUALITY CONTROL
It is recommended to use the Biochemistry Control Serum level I (cod. 18005, 18009 and 18042)
and II (cod. 18007, 18010 and 18043) to verify the performance of the measurement procedure.
Each laboratory should establish its own internal Quality Control scheme and procedures for
corrective action if controls do not recover within the acceptable tolerances.

METROLOGICAL CHARACTERISTICS
− Detection limit: 0.02 mg/dL = 0.00082 mmol/L.
− Linearity limit: 4 mg/dL = 1.64 mmol/L. For higher values dilute sample 1/2 with distilled water
and repeat measurement.

M11512i-0508 BioSystems S.A. Costa Brava 30, Barcelona (Spain)

Quality System certified according to
EN ISO 13485 and EN ISO 9001 standards