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Expt-1-The-Compound-Microscope - Assignment - FRSC3 - CCR 3.B1 - Jerome T. Figuracion

The document summarizes an experiment on using a compound microscope. It describes the objective of studying the parts and correct technique for using a compound microscope. It then lists and explains the functions of the major parts of the compound microscope, including the ocular/eyepiece, revolving nosepiece, objectives, stage, condenser, mirror, and iris diaphragm. Finally, it provides step-by-step instructions for properly using the compound microscope and includes some precautionary measures when using microscopes.

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Gis EL
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116 views4 pages

Expt-1-The-Compound-Microscope - Assignment - FRSC3 - CCR 3.B1 - Jerome T. Figuracion

The document summarizes an experiment on using a compound microscope. It describes the objective of studying the parts and correct technique for using a compound microscope. It then lists and explains the functions of the major parts of the compound microscope, including the ocular/eyepiece, revolving nosepiece, objectives, stage, condenser, mirror, and iris diaphragm. Finally, it provides step-by-step instructions for properly using the compound microscope and includes some precautionary measures when using microscopes.

Uploaded by

Gis EL
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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NAME: JEROME T.

FIGURACION GROUP NUMBER:


__________

YEAR AND SECTION: CCR 3- B1 DATE


PERFORMED: 10.7.2020

PROFESSOR: MS. DEOGRACIA DATE


CORRECTED:_________

EXPERIMENT 1

THE COMPOUND MICROSCOPE

I. OBJECTIVE: TO STUDY THE PARTS AND THE CORRECT TECHNIQUE OF USING THE

COMPOUND MICROSCOPE.

II. MATERIALS: compound microscope, glass slides with specimen

III. DISCUSSION: The compound microscope is an example of a light microscope, a type of


that makes use of a visible light. It consists of two sets of lenses, namely, the primary magnifiers
or the objectives, the lenses of which are immediately next to the specimen being studied; and
the secondary magnifier, that is, the ocular or eyepiece, the lenses of which are next to the eye.

IV. PROCEDURE: Draw and label the parts of the compound microscope.
Experiment 1

V. OBSERVATION/CONCLUSION:

1. Give the function(s) of the following:

a. ocular/eyepiece – where you place your eye

b. revolving nosepiece – the rotating device that holds the objectives (lenses).

c. coarse adjustment knob – a knob that makes large adjustments to the focus.

d. low power objective – a small lens with low magnifying power.

e. high power objective – a large lens with high magnifying power.

Experiment 1

f. stage – the platform on which a slide is placed

g. condenser – are located above the light source and under the sample in an
upright microscope, and above the stage and below the light source in an inverted microscope.
They act to gather light from the microscope's light source and concentrate it into a cone of light
that illuminates the specimen.

h. mirror – this directs light upwards onto the slide.

i. iris diaphragm – controls the size of the opening between the specimen and condenser,
through which light passes.

2. Describe briefly and in a systematic order, the manner or method of properly using the

compound microscope.
Familiarization  First, familiarize yourself with all the parts of a microscope so that you can
easily move from one part to another during operation.
 
Moving and Placement: It is worth remembering that while a good quality microscope will last
a lifetime, it is a sensitive scientific instrument that will suffer damage from sharp blows or
impact. Always, therefore, carry your microscope in both hands. Grasp the arm with one hand
and place the other hand under the base for support. Always place the microscope on a level and
stable surface.

Slide Preparation: Microscope slides should always be prepared with a cover slip or cover glass
over the specimen. This will help protect the objective lenses if they touch the slide. To hold the
slide on the stage fasten it with the stage clips. You can push down on the back end of the stage
clip to open it.

Focusing the Microscope:

      Start by turning the revolving nosepiece (turret) so that the lowest power objective
lens is "clicked" into position. The lowest power objective is the shortest one. This objective
is the easiest to focus and center the image in the field of view.
      While looking at the objective lens and the stage from the side, turn the coarse focus
knob so that the stage moves upward toward the objectives. Move it as far as it will go
without touching the slide.
      Now, look through the eyepiece(s) and adjust the illuminator and diaphragm until you
attain the maximum, comfortable level of light.
      Slowly turn the coarse adjustment so that the stage moves down (away from the slide).
Continue until the image comes into broad focus. The turn the fine adjustment knob, as
necessary, for perfect focus.
      Move the microscope slide until the image is in the center of the field of view. Then
readjust the illuminator or diaphragm in order to attain the clearest image.
      Once you have attained a clear image, you should be able to change to a higher
power objective lens with only minimal use of the focusing adjustment. If you cannot focus
on your specimen, repeat the above steps and work from objective to objective until the
higher power objective lens is in place.

3. Give some precautionary measures in the use of the microscope.


 Clean the microscope after each use.
 Clean smudged lenses with lens paper. Avoid applying pressure
with a cloth as the lenses are very fragile.
 Wipe the stage (the platform that holds the slides) down thoroughly
and disinfect the eyepiece with an alcohol-based wipe.
 Handle glass slides carefully. If a slide breaks, ensure that the contents
are properly disposed and report the incident in PeopleSoft if an injury
occurs.
 Turn off the light source when the microscope is not in use. This will improve lamp
longevity and save energy.
 Be aware if your microscope has a mercury lamp. A broken mercury lamp may release
toxic mercury vapors. You can dispose of any unwanted mercury-containing devices by
submitting a pick-up request to [email protected]
 When carrying the microscope, always use two hands with one hand supporting the base
and theother hand holding the arm.
 Properly store the microscope by lowering the nosepiece, turning off the light source, and
placing the objective lenses on the lowest setting. Cover with a dust jacket.
 Ensure your microscope is scheduled for preventative maintenance and keep the area
around the microscope clean. For more information, consult the manufacturer’s manual for
your equipment

Experiment 1

4. Name the three general parts of the compound microscope and their sub-parts.
 Head/Body houses the optical parts in the upper part of the microscope.
 Base of the microscope supports the microscope and houses the illuminator.
 Arm connects to the base and supports the microscope head.

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