MAPUA UNIVERSITY

School of Chemical, Biological, Material Engineering and

Sciences (CBMES)

Experiment No.: 6
Title: DISSOVLED OXYGEN LEVELS IN NATURAL WATER

Group No.: 8
Name: TEODORO, MIGUEL NOLITO J.

Pre/Post Lab Quiz

Evaluation
Prelim Data Sheet
Laboratory Attire
Title Page
Report Form
Conclusion

MARILYN A. MIRANDA
Instructor
 Experiment 6 REPORT SHEET

Dissolved Oxygen Levels on Natural Waters

A. Sample Analysis

Trial 1 Trial 2 Trial 3

Sample volume (mL) 201 205 205
Buret reading, initial (mL) 0 20.2 24.15
Buret reading, final (mL) 1.4 21.3 25.1
Volume Na2S2O3 dispensed (mL) 1.4 1.1 0.95
Molar concentration of Na2S2O3 0.09955
(mol/L), Part A
Moles of Na2S2O3 dispensed (mol) 1.3937 x 10-4 1.09505 x 10-4 9.4572 x 10-5
Moles of I3 reduced by S2O32- (mol) 6.9685 x 10-5 5.4753 x 10-5 4.7286 x 10-5
Moles of O2 (mol) 3.4843 x 10-5 2.7376 x 10-5 2.3643 x 10-5
Mass of O2 (mg) 1.1149 0.8760 0.7565
Dissolved oxygen, ppm O2 (mg/L) 5.5467 4.2731 3.6904
Average dissolved oxygen, ppm O2 4.5037
Standard deviation 0.9493
Relative standard deviation (%RSD) 21.0790

B. Standard Deviation of Trial 1, Trial 2, and Trial 3

x x̄ x - x̄ Σ (x - x̄)2
5.5467 4.5037 1.043 1.087849
4.2731 4.5037 - 0.2306 0.05317636
3.6904 4.5037 - 0.8133 0.66145689
1.80248225
 Experiment 6 CALCULATIONS

Dissolved Oxygen Levels on Natural Waters

Trial 1

Moles of Na2S2O3 dispensed (mol)

mol 0.09955 mol

1 mol Na2S2O3 = *L= * 0.00140 L = 1.3937 x 10-4 mol
L L
Moles of I3 reduced by S2O32- (mol)
1 mole Na2S2O : 1 mole S2O32-

1 mole S2O32- : 2 moles I3-

1mole I 3
mol I3 = 1.3937 x 10-4 mol S2O32- * L = = 6.9685 x 10-5 mol
2moles S 2 O3−2

Moles of O2

1 mole O2
mol O2 = 6.9685 x 10-5 mol I3- * 2 moles Mn ¿ ¿ * = 3.4843 x 10-5 mol
4 moles Mn ¿ ¿
Mass of O2

Molar mass of O2 = 15.999 g/mol

15.999 g O 2 1000 mg
mass O2 = 3.4843 x 10-5 mol O2 * 2 * * = 1.1149 mg
1 mole O 2 1g

Dissolved oxygen, ppm O2

1.1149 mg
ppm O2 = = 5.5467 ppm
0.201 L
Trial 2

Moles of Na2S2O3 dispensed (mol)

mol 0.09955 mol

1 mol Na2S2O3 = *L= * 0.00090 L = 1.09505 x 10-4 mol
L L
Moles of I3 reduced by S2O32- (mol)
1 mole Na2S2O : 1 mole S2O32-

1 mole S2O32- : 2 moles I3-

1mole I 3
mol I3 = 1.09505 x 10-4 mol S2O32- * L = = 5.4753 x 10-5 mol
2moles S 2 O 3−2

Moles of O2
 1 mole O2
mol O2 = 5.4753 x 10-5 mol I3- * 2 moles Mn ¿ ¿ * = 2.7376 x 10-5 mol
4 moles Mn ¿ ¿

Mass of O2

Molar mass of O2 = 15.999 g/mol

15.999 g O2 1000 mg
mass O2 = 2.7376 x 10-5 mol O2 * 2 * * = 0.8760 mg
1 mole O2 1g

Dissolved oxygen, ppm O2

0.8760 mg
ppm O2 = = 4.2731 ppm
0.205 L
Trial 3

Moles of Na2S2O3 dispensed (mol)

mol 0.09955 mol

1 mol Na2S2O3 = *L= * 0.000950 L = 9.4572 x 10-5 mol
L L
Moles of I3 reduced by S2O32- (mol)
1 mole Na2S2O : 1 mole S2O32-

1 mole S2O32- : 2 moles I3-

1mole I 3
mol I3 = 9.4572 x 10-5 mol S2O32- * L = = 4.7286 x 10-5 mol
2moles S 2 O3−2

Moles of O2

1 mole O2
mol O2 = 4.7286 x 10-5 mol I3- * 2 moles Mn ¿ ¿ * = 2.3643 x 10-5 mol
4 moles Mn ¿ ¿
Mass of O2

Molar mass of O2 = 15.999 g/mol

15.999 g O 2 1000 mg
mass O2 = 2.3643 x 10-5 mol O2 * 2 * * = 0.7565 mg
1 mole O 2 1g

Dissolved oxygen, ppm O2

0.7565 mg
ppm O2 = = 3.6904 ppm
0.205 L
Standard Deviation
 1.80248225
SD = √ Σ¿ ¿ ¿ =
√ 3−1
= 0.9493

Relative Standard Deviation

SD 0.9493
RSD = * 100 = * 100 = 21.0790%
x̄ 4.5037

EXPERIMENT 6 LABORATORY QUESTIONS & CONCLUSION

Dissolved Oxygen Levels on Natural Waters

Laboratory Questions

Part B. Since the water sample is too clean, the dissolved oxygen concentration will be too high.

Part B.3.a. “Fix the dissolve oxygen” means that the dissolved oxygen is allowed to react to form a
compound that will not go away from the solution. The process is important in the analysis of dissolved
oxygen because the dissolved oxygen cannot escape from the solution after fixation. Also, dissolved
oxygen is completely converted to Mn(OH) 3. The amount of dissolved oxygen can be determined by the
amount of Mn(OH)3 formed.

Part B.3.b. An approximate volume of 1 mL of MnSO 4 is required for fixing the dissolved oxygen in the
sample because in the reaction, MnSO 4 is the excess reactant. Meanwhile, the dissolved oxygen is the
limiting reactant.

Part B.4. It is most likely because the reagents were exposed to the environment. They were cooled
enough that their solubility was decreased.

Part B.5. The dissolved oxygen concentration is inversely proportional to the water temperature
because oxygen is less soluble in warm water than in cold water. Since the water chemist measured and
recorded the air temperature which is higher, the reported dissolved oxygen concentration will be
reported as being higher than it should be.
Part C.3. The Mn(OH)2 appears brown in color. Since the color was not achieved, this could mean that
there is a low concentration of dissolved oxygen in the water sample.
7 mg O2 1L 1g 1 mol O2 4 mol Mn ( OH )3
6.a. n Mn (OH ) = x 200 mL x x x x = 1.75 x 10−4
3
1L 1000 mL 1000 mg 32 gO2 1 mol O2
mol

n I −¿
6.b. I −¿
3
=1.75 x 10−4 mol Mn (OH )3 x 1mol 3
=8.75 x10 −5 ¿ ¿mol
2 mol Mn (OH )3

6.c.
n −¿x 2mol S 2 −¿
O2−
3
¿

−4
¿¿ mol
2−¿ −5 1mol I 3 =1.75 x10 ¿
S2 O3 =8.75 x10 mol I 3 ¿
 1L
2−¿ x ¿
2−¿ 1000 mL
6.d. 0.025 mol S2 O3 x
1L
=7 mL¿
V Na S O =1.75 x 10−4 mol S2 O 3
2 2 3

7. Marine life can adapt to changes in their environment like the rise in temperature or lowered
dissolved oxygen levels. In this case, a test for dissolved oxygen levels could not be a reliable test in
determining the cause. However, a rise in temperature can lead to algal bloom and eutrophication
induces further growth where water becomes filled with too much minerals. This causes fish kill.

8.a. Water is usually used as a coolant. When it is discharged and returned to the environment at a high
temperature, the change in temperature would decrease the oxygen supply because dissolved oxygen
concentration is inversely proportional to the water temperature. This will subsequently lead to fish kill.

8.b. This is due low oxygen content in water. Stale water is sinks to the bottom of the pond and unable
to mix with the surface. Stale water has no oxygen and contains hydrogen sulfide. However, during
heavy rainfall, this stagnant stale water mixes with the fresh water supply, resulting in a lower dissolved
oxygen concentration and subsequently, fish kill.

9. Dissolved oxygen concentrations are higher at the headwaters of a river and go lower as it goes down
to the ocean because of the presence of bacteria and other species that consume oxygen. There is a
higher number of bacteria in the rivers than in the oceans.

10. Higher levels of salinity can cause lower levels of dissolved oxygen concentrations. Therefore,
saltwater has a lower oxygen concentration than freshwater.
Conclusion

Total dissolved oxygen in water provides life to the numerous aquatic lives in earth. The
experimentation done is a reflection on how the total dissolved water in natural water is measured. The
data measured can specify what type of aquatic creature can survive in specified amount of dissolve
oxygen in the water.