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Filters For Epi Illuminator

The document provides instructions for using various components of a microscope, including: - Connecting the power cord and adjusting brightness settings. - Using coarse and fine focusing knobs to focus the microscope and adjusting the coarse focus torque. - Utilizing tools like the coarse focus quick stop, beam splitter lever, and diopter adjustment rings. - Properly inserting filters and installing the Epi illuminator horizontally for best image quality. - Centering the field diaphragm and adjusting the polarizer and analyzer settings when using Epi illumination.

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LESO Industrial
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26 views

Filters For Epi Illuminator

The document provides instructions for using various components of a microscope, including: - Connecting the power cord and adjusting brightness settings. - Using coarse and fine focusing knobs to focus the microscope and adjusting the coarse focus torque. - Utilizing tools like the coarse focus quick stop, beam splitter lever, and diopter adjustment rings. - Properly inserting filters and installing the Epi illuminator horizontally for best image quality. - Centering the field diaphragm and adjusting the polarizer and analyzer settings when using Epi illumination.

Uploaded by

LESO Industrial
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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 Plug the power cord from lamp house to the outlet on the rear panel of the microscope.

(Fig.4)

 For the best image quality, install the Epi illuminator horizontally.

9. Filters for Epi illuminator


 Pull out the slider on Epi illuminator
 Place the filter and/or ground glass in the filter holder on the slider and make sure the frosted side
of ground glass is faced to the lamp.
 Push the slider to make sure the filter/ground glass stay in the optical path.

10. Filters for transmitted illumination


 Remove the collector cover (Fig.5) and place the filter in the filter holder located around the field
lens, then screw the cover. Take care that dust, dirt and fingerprints do not get on the filter and
the field lens.

(Fig.5)

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 Filter selection:
Filter Function
ND2 (T=50%)
ND4 (T=25%) For brightness adjustment in photomicrography
ND16 (T=6.25%)
Blue filter (colour balance filter) For routine microscopy and photomicrography
For phase contrast and contrast adjustment with
Green interference (546nm)
black and white film

For colour photomicrography of HE stained


HE (didymium filter)
specimen with tungsten type film

 A diffuser is built into the base of the microscope.

11. Power cord


Connect the socket of the of the power cord to the AC inlet on the rear of the base of the microscope.
Plug in the other end of the cord to an AC outlet with ground conductor.

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IV. MICROSCOPY

1. Illumination brightness adjustment


 Turn the brightness adjustment knob fully counterclockwise to the low brightness position.
 Set the power switch to “I” (ON).
The green line control lamp in the switch must light up.
The halogen lamp 12V/50 W in the Epi illuminator must light up.
 When the Brightness adjustment knob is turned clockwise to the high brightness position, the light
intensity increases.

MRL100 Power Unit

2. Coarse and fine focusing


 Focusing is carried out with the coarse and fine focus knobs at the left and right of the microscope
stand.
 The direction of vertical movement of the stage corresponds to the turning direction of the focus
knobs.
 One rotation of the fine focus knob moves the stage 0.2mm. The graduation on the fine focus knob
is 2 microns.

Never attempt either of the following actions, since doing so will damage the focusing
mechanism:
 Rotate the left and right knob while holding the other.
 Turning the coarse and fine focus knobs further than their limit.

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3. Coarse focus torque adjustment
 To increase the torque, turn the torque adjustment ring (Fig.7) located behind the left-hand coarse
focus knob in the direction indicated by the arrow. To reduce the torque, turn the ring in the
direction opposite to that indicated by the arrow.

(Fig.7)

4. Coarse focus quick stop


 The coarse focus quick stop (Fig.8) makes the stage can fixed at any position at which the
specimen is in focus i.e. by using the handle to lock the coarse focus knob.
 With the specimen in focus, turn the handle to fix the knob.
 When the coarse focus quick stop is in position, the stage cannot be raised from that position.
However, the fine focus knob can move the stage regardless of the limit but will only lower the
stage.
 Lower the stage by using the coarse focus knob.

(Fig.8)

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5. Beam splitter lever
 The beam splitter lever of the trinocular eyepiece tube can be used to select the amount of light
distributed between the trinocular eyepiece tube and the vertical phototube.
 When the lever is pushed in until it reaches the limit, 100% of the light enters the observation tube.
When the lever is pulled out to the limit, the ratio of light entering the observation tube and
phototube will be 20:80.

6. Interpupillary distance adjustment


 Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.
 Adjust the interpupillary distance (Fig.9) so that both the right and left field of view become one
 This adjustment will enable the user to observe the specimen with both eyes

(Fig.9)

7. Diopter adjustment
 Diopter adjustment compensates for the differences in vision between the left and right eyes. In
addition to making observation through both eyes easier, this adjustment also reduces the extent to
which focusing is lost when the objective magnification is changed. In particular, this occurs when a
low magnification objective is used.
 The left eyepiece has a separate focusing provision to compensate for slight differences in the
focusing of each eye.
 Using the right eye only and viewing through the right-hand eyepiece, adjust the focus with the
microscope fine or coarse adjustment until the image of the specimen is at its sharpest.
 Using the left eye only and viewing through the left-hand eyepiece with its independent diopter-
focusing ring, focus until the specimen image is at its sharpest.
 The microscope should now be ready for binocular viewing.

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(Fig.10)

8. Polariser and Analyser of Epi-illuminator using


 Insert the polariser (marked with “P”) into the front slot of Epi.
 Insert the Analyser (marked with “A”) into the side slot of Epi.
 Analyser is rotatable and the color of specimen with polarization will be changed when rotating.

(Fig.11)

9. Field diaphragm of Epi-illuminator centering


 Clear diaphragm image can be obtained after the specimen is in focus.
 Adjust the aperture diaphragm until aperture diaphragm is 2/3 of field then center the aperture
diaphragm via the knurled knob on the top of Epi illuminator.
 Set the aperture diaphragm slightly bigger than the field of view by turning the adjustment wheel.

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