MEGHNA BEVERAGE LIMITED.

TOPORDI, SONARGOAN,nARAYANGONJ

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 1

MATERIAL NAME: CITRIC ACID ANHYDROUS

Molecular Formula: C6H8O7 Formula Weight: 192.13 Equivalent Weight: 64.03

SL Test Parameters Specification

NO.
1. Physical State Colorless crystal or White Crystalline Powder
A neutral solution of citric acid is boiled with HgSO4 TS (5 g in 80 ml
2. Identification of water), then filtered. Few drops of KMnO4 TS (10 g/l) is added to
the filtrate, a white precipitate is formed.
Clarity 10 % W/W Solution Should Be Clear Compared With Existing Good
3.
One
4. Loss on drying NMT 1.0% w/w at 105°C for 15 minutes, use 2 g
5. Assay (%) 99.5 % to 100.5 % as C6H8O7 calculated on anhydrous basis.

METHOD OF ASSAY ANALYSIS

1. Place About 0.55 g Of Anhydrous Citric Acid In A Tarred Conical Flask &Weigh Accurately.
2. Dissolve In 50ml Of Water.
3. Add 0.5ml Of Phenolphthalein TS.
4. Titrate With 1N Sodium Hydroxide VS.
5. End Point Is Determined When Just Pink Color Appeared.

CALCULATION OF ASSAY PERCENTAGE

Each ml Of 1N Sodium Hydroxide Is Equivalent To 64.03 mg Of C 6H8O7
Or
BR × Normality Of NaOH ×Equivalent Weight Of Citric Acid(64.03)×100
Sample Weight×1000

Precautions:
1. Standardize Sodium Hydroxide Solution Before Titration.
2. Weighing Should Be Accurate

REFERENCE: USP-29

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 MEGHNA BEVERAGE LIMITED.
TOPORDI, SONARGOAN,nARAYANGONJ

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 2

MATERIAL NAME: TRI SODIUM CITRATE DIHYDRATE

Molecular Formula: C6H5Na3O7.2H2O Formula Weight: 294.10 Equivalent Weight: 86.02

SL Test Parameters Specification

NO.
1. Physical State Colorless crystal or White Crystalline Powder
A solution of sodium citrate is boiled with HgSO4 TS (5 g in 80 ml of
2. Identification water), then filtered. Few drops of KMnO4 TS (10 g/l) is added to the
filtrate, a white precipitate is formed.
Clarity 10 % W/W Solution Should Be Clear Compared With Existing Good
3.
One
4. Assay (%) 99.0 % to 100.5 % as C6H8O7 calculated on anhydrous basis.

METHOD OF ASSAY ANALYSIS

1. Place About 0.35 g Of sample previously dried at 180°c for 18 hours & Weighed Accurately to a
250 ml conical flask.
2. Add 100 ml of glacial acetic acid & dissolve properly.
3. Titrate With 1.0 N Perchloric acid VS using crystal violet TS, until bluish green color appeared
4.Perform a blank titration

CALCULATION OF ASSAY PERCENTAGE

Each ml Of 0.1N perchloric acid is Equivalent To 8.602 mg Of C6H5Na3O7
i.e
BR × Normality Of perchloric acid ×Eq.wt. Of sodium citrate(86.02)×100
Sample Weight×1000

Precautions:
1. Standardize per chloric acid Solution before Titration.
2. Weighing Should Be Accurate

REFERENCE: USP-29

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 MEGHNA BEVERAGE LIMITED.
TOPORDI, SONARGOAN,nARAYANGONJ

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 3

MATERIAL NAME: SODIUM BENZOATE

Molecular Formula: C7H5NaO2 Formula Weight: 144.10 Equivalent Weight: 144.1

SL Test Parameters Specification

NO.
1. Physical State White Crystalline or granular Powder
1. When a solution of benzoate (1g in 20 ml of water) is
acidified with dilute HCl, crystalline ppt is formed.
2. Identification 2. When FeCl3 solution is added to a neutral solution of
benzoate (1g in 20 ml of water), reddish brown ppt is
formed.
Clarity 5 % W/W Solution Should Be Clear Compared With
3.
Existing Good One
4. Loss on drying NMT 2.0% w/w at 105°C for 15 minutes, use 1 g
5. Assay (%) 99.0 % to 100.5 % as C6H8O7 calculated on anhydrous basis.

METHOD OF ASSAY ANALYSIS

1. Place About 0.6 g Of Accurately Weighed sample to a 250 ml conical flask.
2. Add 100 ml of glacial acetic acid & dissolve properly.
3. Add 2 drops of crystal violate TS.
3. Titrate With 0.1 N Perchloric acid VS untill green color appeared.
4. Perform a blank titration .

CALCULATION OF ASSAY PERCENTAGE

Each ml Of 0.1 N perchloric acid Is Equivalent To 14.41 mg Of C7H5NaO2

Precautions:
1. Standardize Perchloric acid Solution Before Titration.
2. Weighing Should Be Accurate

REFERENCE: USP-29

MATERIAL NAME: COLA KIT PART-02 (PHOSPHORIC ACID)

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 4

Molecular Formula: H3PO4 Formula Weight: 98 Equivalent Weight: 49

SL Test Parameters Specification

NO.
1. Physical State Liquid could crystalize
2. Color Colorless
Identification When silver nitrate solution is added to a neutral solution of H3PO4,
3.
yellow color precipitate is formed.
4. Specific gravity(d20/20) 1.686 to 1.730
5. Refractive index(nd/20) 1.431 to 1.434
6. Acid content 84.0 % to 86.0 % as H3PO4

METHOD OF ASSAY ANALYSIS

1. Weigh accurately about 1 g Of phosphoric acid in a tared glass stoppered flask & dilute it with 120
ml of water.
2. Add 0.5 ml of Phenolphthalein TS.
3. Titrate with 1 N NaOH VS Until blue color is appeared.
4. Perform a blank titration.

CALCULATION OF ASSAY PERCENTAGE

Each ml Of 1N NaOH is Equivalent To 49 mg Of H3PO4
i.e
BR × Normality Of NaOH ×Eq.wt. Of Phosphoric acid (49)×100
Sample Weight×1000

Precautions:
1. Standardize NaOH Solution Before Titration.

2. Weighing Should Be Accurate

REFERENCE: USP-29

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 5

MATERIAL NAME: FERROUS SULFATE HEPTAHYDRATE

Molecular Formula:FeSO4.7H20 Formula Weight: 278.02 Equivalent Weight: 278.2 g

SL Test Parameters Specification

NO.
1. Physical State Light green Crystalline powder
When NaOH solution is added to ferrous salt solution, gel like white
Identification ppt is formed. If this is well shaken, the color becomes greyish &
2.
gradually turns reddish brown. When sodium sulfide solution is
added, the color of ppt changes to black.
3. pH 3.0 to 4.0 of 5% solution
4. Assay (%) 99.5% to 104.5 % as FeSO4.7H20

METHOD OF ASSAY ANALYSIS

1. Take about 0.5 g of accurately weighed sample.
2. Transfer it in a mixture of 25 ml of diluted sulfuric acid & 25 ml of freshly boiled & cooled water.
3. Titrate with 0.1 N KMnO4

CALCULATION OF ASSAY PERCENTAGE

Each ml Of 0.1N KMnO4 Is Equivalent To 27.8 mg Of FeSO4.7H20
i.e
BR × Normality Of KMnO4 ×27.8
Sample Weight

Precautions:
1. Standardize KMnO4 Solution Before Titration.
2. Weighing Should Be Accurate

REFERENCE: USP29

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 6

MATERIAL NAME: CALCIUM HYPOCHLORITE (BLEACHING POWDER)

Molecular Formula: Ca(Ocl)Cl Formula Weight of chlorine: Equivalent Weight: 35.46 g
35.46

SL Test Parameters Specification

NO.
1. Physical State White granular powder
When 2 ml of acetic acid to 0.1 g of bleaching powder, gas is
Identification evolved & it dissolves. 5 ml of water is added then filtered.
2.
Ammonium oxalate solution ( 1 g in 30 ml of water) is added to the
filtrate with HCl, white ppt is formed.
3. Purity (%) 65% to 70 % as available chlorine

METHOD OF ASSAY ANALYSIS

1. 5 gm of sample is weighed accurately & mixed with 500 ml of water using a morter.
2. Transfer 25 ml 0f stock solution into a conical flask.
3. Add 2g KI+10ml glacial acetic acid.
4. Titrate with 0.1N Na2S2O3 using starch indicator until colorless solution is appeared.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 0.1 N Na2S2O3 is equivalent to 3.546 mg of Cl2
Calculation(% available Cl2): BR×0.003546gm×100/0.25

Precautions:
1. Standardize Na2S2O3 Solution Before Titration.
2. Weighing Should Be Accurate

REFERENCE: USP29

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TOPORDI, SONARGOAN,nARAYANGONJ

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 7

MATERIAL NAME: CALCIUM OXIDE (LIME)

Molecular Formula: CaO Formula weight: 56.08 Equivalent Weight: 28.04

SL Test Parameters Specification

NO.
1. Physical State White hard lump
20 ml of water is added to 1 g of CaO. Acetic acid is added until it
2. Identification dissolves, then filtered. Ammonium oxalate solution ( 1 g in 30 ml of
water) is added to the filtrate with HCl, white ppt is formed.
3. Assay (%) Minimum 66% as CaO calculated as anhydrous basis.

METHOD OF ASSAY ANALYSIS

1. Ignite about 1 g of sample to constant weight, cool, weigh accurately.
2. Dissolve in 20 ml of 3N HCl. Cool the solution.
3. Transfer to a 500 ml volumetric flask & up to the mark with water.
4. Take 50 ml of stock solution & add 100 ml of water,15 ml of 1N NaOH & 300 mg of Hydroxy
naphthol blue.
5.Titrate with 0.05M EDTA VS until the solution is deep blue in color

CALCULATION OF ASSAY PERCENTAGE

Each ml of 0.05M EDTA is equivalent to 2.804 mg of CaO

Precautions:
1. Standardize EDTA Solution Before Titration.
2. Weighing Should Be Accurate

REFERENCE: USP-29

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 8

MATERIAL NAME: SODIUM CHLORIDE

Molecular Formula: NaCl Formula weight: 58.44 Equivalent Weight: 58.44

SL Test Parameters Specification

NO.
1. Physical State White crystalline powder
It responds the test for sodium & chloride.
Chloride: Dissolve about 3 mg of NaCl in 2 ml of water. Acidify
2. Identification with dilute nitric acid & add 0.4 ml of AgNO3 TS. Shake & allow to
stand. A curdled, white ppt is formed, which is dissolve by adding
excess amount of ammonia solution.
3. Solubility Soluble in water but insoluble in absolute ethanol.
4. Assay (%) 99.0 % to 100.5%(refined) & 96% minimum for commercial grade

METHOD OF ASSAY ANALYSIS

1. Dissolve 0.05 g of sample & dilute with 100 ml of water.
2. Add 2 or 3 drops of potassium chromate TS.
2. Titrate with 0.1 N Silver Nitrate VS, until brick red color is appeared.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 0.1 N silver nitrate is equivalent to 5.844 mg of NaCl
Or
BR × Normality Of silver nitrate ×5.844
Sample Weight

Precautions:
1. Standardize silver nitrate Solution Before Titration.
2. Weighing Should Be Accurate

REFERENCE: USP-29

MATERIAL NAME: ASCORBIC ACID (VITAMIN-C)

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 MEGHNA BEVERAGE LIMITED.
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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 9

Molecular Formula: C6H8O6 Formula weight: 176.12 Equivalent Weight: 88.06

SL Test Parameters Specification

NO.
1. Physical State Colorless or white crystalline powder
When 1 ml of potassium permanganate solution is added to 10 ml
2. Identification of an aqueous solution of vitamin-c ( 1 g in 100 ml water), the color
disappears immediately.
3. pH 2.1 to 2.6 of 5% w/v solution
4. Solubility Soluble in water & ethanol but insoluble in Chloroform & ether
5. Assay (%) 99% to 100.5 % as C6H8O6 calculated as anhydrous basis.

METHOD OF ASSAY ANALYSIS

1. Dissolve about 0.4 g accurately weighed sample.
2. Add 100 ml water & 25 ml of 2N Sulfuric acid.
3. Add 3 ml starch solution TS.
4. Titrate at once with 0.1 N Iodine solution VS,until violet blue color is obtained.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 0.1 N Iodine is equivalent to 8.806 mg of C6H8O6
or
BR × Normality Of iodine solution ×8.806
Sample Weight

Precautions:
1. Titrate with iodine instantly.
2. Standardize iodine solution before titration.
3. Weighing should be accurate.
4. Handle iodine solution carefully.

REFERENCE: USP-29

MATERIAL NAME: ASPARTAME

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 MEGHNA BEVERAGE LIMITED.
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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 10

Molecular Formula: C14H18N2O5 Formula weight: 294.30 Equivalent Weight: 294.30

SL Test Parameters Specification

NO.
1. Physical State White crystalline powder
Dissolve 20 mg of aspartame in 1 ml of methanol, add 0.5 ml of
methanol saturated with hydroxylamine hydrochloride. Mix, then
2. Identification add 0.3 ml of 35 % methanolic KOH solution & boil. cool then adjust
the pH with 0.1N HCl. Then add FeCl3 solution (1 g in 100 ml of
water). A deep red purple color develops.
3. Taste Has a strong sweet taste
4. pH 4.5 to 6.0 (0.8 % solution)
5. Assay (%) 98 to 102.0 % of C14H18N2O5 Calculated on anhydrous basis.

METHOD OF ASSAY ANALYSIS

1. Accurately weighed 0.3 g of sample.
2. Dissolve in 1.5 ml of formic acid.
3. Add 60 ml of glacial acetic acid then add crystal violet TS.
4. Immediately titrate with 0.1 N perchloric acid VS to a green end point.
5. Perform a blank determination.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 0.1 N Perchloric acid is equivalent to 29.43 mg of C 14H18N2O5
Or
BR(blank-sample) × Normality Of Perchloric acid ×29.43
Sample Weight

Precautions:
1. Standardize Perchloric acid Solution Before Titration.
2. Weighing Should Be Accurate.

REFERENCE: USP-29

MATERIAL NAME: ACESULFAME-K

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 11

Molecular Formula: C4H4NO4SK Formula weight: 201.24 Equivalent Weight: 201.24

SL Test Parameters Specification

NO.
1. Physical State White crystalline powder
0.2 g of sample is dissolved in 2 ml of acetic acid (30 ml in 100 ml of
2. Identification water), & 2 ml of water. Upon adding a few drops of sodium cobalt
nitrite solution, yellow ppt are formed.
3. Taste Has a strong sweet taste
4. Moisture 0.2 maximum
5. pH 5.5 to 7.5 (1% solution)
6. Assay (%) 99.0 to 101.0 % of C4H4NO4SK

METHOD OF ASSAY ANALYSIS

1. Dissolve about 0.15 g of sample in 50 ml of glacial acetic acid.
2. Add crystal violet TS.
3. Immediately titrate with 0.1 N perchloric acid VS to a bluish green end point.
4. Perform a blank determination.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 0.1 N Perchloric acid is equivalent to 20.12 mg of C4H4NO4SK
Or
BR( blank – sample) × Normality Of Perchloric acid ×20.12
Sample Weight

Precautions:
1. Standardize Perchloric acid Solution Before Titration.
2. Weighing Should Be Accurate.

REFERENCE: USP-29

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 12

MATERIAL NAME: RBD PALM OLEIN

SL Test Parameters Specification

NO.
1. Physical State Liquid
Color Pale yellow
2. Peroxide value Maximum 1 meq/kg
3. FFA value Maximum 0.2 %
4. Odor No off odor found

METHOD OF PEROXIDE VALUE ANALYSIS

1.Take 5gm sample then add 30ml acetic acid: chloroform solution(480 ml acetic acid & 320 ml
chloroform)
2. Add 0.5ml KI solution, stopper the flask & swirl exactly 1 min.
3. Add 30 ml of water. Shake vigorously to liberate iodine from the chloroform layer. Deep orange
color appeared.
4. Titrate with 0.1 N Na2S2O3 VS.
5. If initial color is deep orange, titrate slowly until the color lighten using starch indicator (1%). If
initial color is light amber, titrate until blue gray color disappear in upper layer.
6. Conduct a blank titration of the reagent.

CALCULATION OF PEROXIDE VALUE

Calculation: BR (Titration of sample – Titration of blank)× Normality Of Na2S2O3 ×1000

Sample Weight

Precautions:
1. Standardize Na2S2O3 Solution Before Titration.
2. Weighing Should Be Accurate

REFERENCE-AOCS (AMERICAN OIL CHEMIST’S SOCIETY)

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 13

MATERIAL NAME: RBD PALM OLEIN

SL Test Parameters Specification

NO.
1. Physical State Liquid
Color Pale yellow
2. Peroxide value Maximum 1 meq/kg
3. FFA value Maximum 0.2 %
4. Odor No off odor found
METHOD OF FREE FATTY ACID ANALYSIS
1. Take 10 g of oil sample
2. Add 10 g of methanol to dissolve the sample.
3. Add 2 to 3 drops of phenolphthalein TS.
4. Titrate with 0.025 N NaOH VS.
5. Conduct a blank titration.

CALCULATION OF FFA PERCENTAGE

% FFA= BR (Titration of sample – Titration of blank)× Normality Of NaOH ×28.2
Sample Weight
OR
% FFA= 0.766× T (Titration result in ml) for NaOH

NOTE: A. 28.2 is the equivalent weight of oleic acid. That is 282/10= 28.2
B. 1 ml of oil= 0.92 g

Precautions:
1. Blank titration must be done.
2. NaOH Should be standardized.

REFERENCE: PORAM (THE PALM OIL REFINERS ASSOCIATION OF MALAYSIA)

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 14

MATERIAL NAME: NITRIC ACID (COMMERCIAL GRADE)

Molecular Formula: HNO3 Formula weight: 63.01 Equivalent Weight: 63.01

SL Test Parameters Specification

NO.
1. Physical State Clear, Colorless fuming liquid
When ferrous sulfate solution is added on the top of a cooled
2. Identification mixture (1:1) of nitrate solution & sulfuric acid, a dark brown band
is formed at the interface.
3. Specific gravity 1.41
4. Purity (%) 68.0 to 71.0 %

METHOD OF ASSAY ANALYSIS

1. Weigh accurately 2 ml of HNO3 in a conical flask.
2. Add 25 ml of water.
3. Add methyl red TS.
4. Titrate with 1 N NaOH VS.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 1 N NaOH is equivalent to 63.01 mg of HNO3
Or
BR × Normality Of NaOH solution ×6.301
Sample Weight

Precautions:
1. Standardize NaOH Solution Before Titration.

2. Weighing Should Be Accurate

REFERENCE: USP-29

MATERIAL NAME: SODIUM HYDROXIDE

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 15

Molecular Formula: NaOH Formula weight: 40.0 Equivalent Weight: 40.0

SL Test Parameters Specification

NO.
1. Physical State White crystalline pallets, flakes
It respond the test for sodium.
2.
Sodium: Acidify the solution with acetic acid (60 g/l). filter if
Identification necessary & treat with zinc acetate TS. A yellow crystalline ppt is
formed.
3. Purity (%) 97.0 to 100.5 % of total alkali

METHOD OF ASSAY ANALYSIS

1. Dissolve 1.5 g of sample in 40 ml of carbon dioxide free water.
2. Cool the solution to room temperature.
3. Add phenolphthalein TS.
4. Titrate with 1 N H2SO4 VS.
5. At The discharge of pink color , record the volume of acid required.
6. Add methyl orange TS & Continue titration until a persistent pink color is produced.

CALCULATION OF ASSAY PERCENTAGE

BR(Using Phenolphthalein TS) × Normality Of H2SO4 solution ×4

Sample Weight
&
BR(Using Methyl Orange TS) × Normality Of H2SO4 solution ×10.6
Sample Weight

Precautions:
1. Standardize H2SO4 Solution Before Titration.

2. Weighing Should Be Accurate

REFERENCE: USP-29

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 16

MATERIAL NAME: SODIUM CARBONATE

Molecular Formula: Na2CO3 Formula weight: 105.99 Equivalent Weight: 52.99

SL Test Parameters Specification

NO.
1. Physical State A white, slightly granular powder
2. Loss on drying Not more than 1.0 %, use 1 g.
3. Assay (%) 99.5 % to 100.5 % of Na2CO3 as dried basis.

METHOD OF ASSAY ANALYSIS

1. Dissolve 1 g sample in 25 ml of water.
2. Add 0.2 ml of methyl orange TS.
3. Titrate with 1M HCl VS until color changes from yellow to red.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 1 N HCl is equivalent to 52.99 mg of Na2CO3
Or
BR × Normality Of HCl solution ×5.299
Sample Weight

Precautions:
1. Standardize HCl Solution Before Titration.

2. Weighing Should Be Accurate

REFERENCE: BP 1993

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 17

MATERIAL NAME: HYDROCHLORIC ACID

Molecular Formula: HCl Formula weight: 36.46 Equivalent Weight: 36.46

SL Test Parameters Specification

NO.
1. Physical State A Clear, colorless, fuming liquid.
It responds the test for chloride.
Chloride: Dissolve about 3 mg of sample in 2 ml of water. Acidify
2. Identification with dilute nitric acid & add 0.4 ml of AgNO3 TS. Shake & allow to
stand. A curdled, white ppt is formed, which is dissolve by adding
excess amount of ammonia solution.
3. Specific gravity 1.18
4. Purity (%) 35 to 39 % w/w. minimum 30% for commercial grade.

METHOD OF ASSAY ANALYSIS

1. Weight 1.5 ml sample into a glass stoppered flask containing 30 ml of water.
2. Add methyl red TS.
3. Titrate with 1M NaOH VS.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 1 N NaOH is equivalent to 36.46 mg of HCl.
Or
BR × Normality Of NaOH solution ×3.64
Sample Weight

Precautions:
1. Standardize NaOH Solution Before Titration.

2. Weighing Should Be Accurate

REFERENCE: BP 1993

MATERIAL NAME: SODIUM BICARBONATE (BAKING POWDER)

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 18

Molecular Formula: NaHCO3 Formula weight: 84.01 Equivalent Weight: 84.01

SL Test Parameters Specification

NO.
1. Physical State White crystalline powder or crystalline lumps
When magnesium sulfate solution is added to bicarbonate solution
2. Identification (1 g in 100 ml of water), white ppt is formed. When dil. Acetic acid is
added, the ppt dissolves.
Clarity When 1 g of sample is dissolve in 20 ml of water, the solution
3.
should be clear.
4. Assay (%) 99 % to 100.5% calculated as anhydrous basis.

METHOD OF ASSAY ANALYSIS

1. Weigh about 3 g of sample, mix with 100 ml of water.
2. Add methyl red TS.
3. Titrate with 1 N HCl VS.
4. Add the acid slowly with constant stirring until the solution become faintly pink.
5. Heat the solution to boil, then cool.
6. Continue the titration until the faint pink color no longer fades after boiling.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 1 N HCl is equivalent to 84.01 mg of NaHCO3
Or
BR × Normality Of HCl solution × 8.401
Sample Weight

Precautions:
1. Standardize HCl Solution Before Titration.

2. Weighing Should Be Accurate.

REFERENCE: USP-29

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 19

MATERIAL NAME: SULFURIC ACID

Molecular Formula: H2SO4 Formula weight: 98.08 Equivalent Weight: 49.04

SL Test Parameters Specification

NO.
1. Physical State A Clear, colorless, fuming liquid.
Identification When BaCl2 solution is added to sulfate solution, white ppt is
2.
formed.
3. Specific gravity 1.84
4. Purity (%) 97 % to 98 % w/w

METHOD OF ASSAY ANALYSIS

1. Place about 1 ml of accurately weighed sample in a glass stoppered flask containing 20 ml of
water.
2. Again weigh to obtain the weight of test specimen.
3. Dilute to about 25 ml of water, then cool.
4. Add methyl orange TS.
5. Titrate with 1N NaOH VS.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 1 N NaOH is equivalent to 49.04 mg of H2SO4
Or
BR × Normality of NaOH solution ×4.904
Sample Weight

Precautions:
1. Standardize NaOH Solution Before Titration.

2. Weighing Should Be Accurate

REFERENCE: USP-29

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 20

MATERIAL NAME: HYDROGEN PEROXIDE

Molecular Formula: H2O2 Formula weight: 34.01 Equivalent Weight: 17.005

SL Test Parameters Specification

NO.
1. Physical State A Clear colorless liquid
To a 1:1 mixture of ethyl acetate & peroxide solution, 1-2 drops of
potassium bicromate solution is added. When the solution is
2. Identification acidified with dil. H2SO4 , the aqueous layer becomes blue.when the
mixture is shaken & settled to separate, the blue color migrates to
ethyl acetate layer.
3. Specific gravity 1.11
4. Purity (%) 30 % contain 29 % to 32% w/w of H2O2

METHOD OF ASSAY ANALYSIS

1. Accurately weighed about 1 ml of sample & dilute it to 100 ml with water.
2. Take 20 ml of stock solution & add20 ml of 2 N sulfuric acid.
3. Titrate with 0.1 N KMnO4 VS.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 0.1 N KmnO4 is equivalent to 1.701 mg of H2O2
Or
BR × Normality Of KmnO4 solution ×1.701
Sample Weight

Precautions:
1. Standardize KmnO4 Solution Before Titration.

2. Weighing Should Be Accurate.

REFERENCE: BP 1993
ACTIVATED CARBON

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SL Test Parameters Specification
NO.
Physical State Black fine powder for sugar plant
1. &
Black granular powder for WTP
2. Moisture ≤10 % for sugar plant & ≤5.0 % for WTP
3. pH 2% to 5% for sugar plant (1 % solution)
4. Iodine value Minimum 850 mg/g
NOTE: Specification may change based on COA & Supplier.

METHOD OF IODINE VALUE ANALYSIS

Blank Titration
1. Take 10 ml of 0.1 N Iodine solution.
2. Add 2 drops of 1% starch solution.
3. The pale yellow color of iodine solution turns blue.
4. Titrate with 0.05 N sodium thiosulfate till it becomes colorless.
5. This burette reading corresponds to (B).
Sample titration
1. Weigh very accurately 0.2 g of activated carbon.
2. Introduce it into completely dry iodine flask.
3. Add 40 ml of 0.1 N Iodine solution.
4. Shake the flask for 4 minutes and filter it.
5. Collected the filtrate in a dry flask.
6. Titrate 10 ml of the filtrate against 0.05 N thiosulfate solution.
7. Burette reading corresponds to (A).

CALCULATION OF ASSAY PERCENTAGE

Iodine value= C (B-A)×Conversion factor
Here, Factor= Molecular weight of iodine (127) × Normality of iodine×40/ Weight of carbon × blank
reading.
Note: Iodine value is expressed in mg/g of carbon

REFERENCE: The complete guide of water chemistry & treatment. Writer: T.P.
Sivanandan

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 22

MATERIAL NAME: STANDARD SUPERCEL FILTER AID

SL Test Parameters Specification

NO.
1. Physical State Creamy powder
2. Moisture 0.7 % at 105°C for 15 minutes
3. Bulk density 0.34 g/ml

MATERIAL NAME: HYFLO SUPERCEL FILTER AID

SL Test Parameters Specification

NO.
1. Physical State Creamy powder
2. Moisture 0.4 % at 105°C for 15 minutes
3. Bulk density 0.35 g/ml
4. pH <10

REFERENCE: COA BASED

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 23

REFINED SUGAR

SL Test Parameters Specification

NO.
1. Physical State White crystal
2. Moisture 0.05 % for Igloo & 0.04 % for Teer sugar
3. Purity 99.85 % minimum for Igloo & 99.82 % for Teer sugar
4. Conductivity ash % <0.016 % for Igloo & <0.0051 % for Teer sugar
5. Color ICUMSA <70 (IU) for Igloo & 38(IU) for Teer sugar
6. Clarity 50% w/w sugar solution should be clear.

METHOD OF PURITY TEST

Take 15 g sugar & 85 g water. It will be 15° brix solution.
Purity %= Obtained brix × 100/15

METHOD OF ASH TEST

Take 28 g sugar & 72 g water. It will be 28° brix solution.
Purity %= Conductivity of sugar solution – Conductivity of distilled water/1666.7

REFERENCE: AMSRL & CSIL

P3-TOPAX 56 ( ACIDIC CLEANING AGENT FOR FOAM CLEANING)

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SL Test Parameters Specification

NO.
1. Appearance Clear colorless liquid
Identification When silver nitrate solution is added to a neutral solution of H3PO4,
2.
yellow color precipitate is formed.
3. Specific gravity 1.160 – 1.200
4. Viscosity 4.0 to 6.0 mPas (20°C)
5. Specific conductivity 5.8 mS/cm ( 1%, 20°C)
6. pH 1.9 to 2.1 (1%, 20°C)

METHOD OF CONCENTRATION ANALYSIS AS PHOSPHORIC ACID

1. Take 50 ml of sample.
2. Add phenolphthalein TS.
3. Titrate with 1.0 N NaOH VS.

CALCULATION OF CONCENTRATION PERCENTAGE

BR ×0.26= % ( by weight) Active as H3PO4
0.26= Titration factor

RECMMENDED USE % = 2 to 5 % with contact time 10 to 15 minutes.

REFERENCE: ECOLAB

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 25

P3-TRIQUART (NEUTRAL DISINFACTANT)

SL Test Parameters Specification

NO.
1. Appearance Clear colorless to light yellow liquid
2. Specific gravity 0.980 to 1.000
3. Refractive index 1.3960 to 1.4020
4. °Brix 38.0 to 41.0
5. pH 7.00 to 8.50 (100% solution)

RECMMENDED USE % = 0.1 to 0.3 % with contact time 1 to 4 hours.

P3-LUBODRIVE (LIQUID SOAP FREE LUBRICANT FOR CHAIN CONVEYORS)

Molecular Formula: Formula weight: Equivalent Weight:

SL Test Parameters Specification

NO.
1. Appearance Clear yellowish liquid
2. Specific gravity 0.980 to 1.020
3. pH 6.40 to 6.80 (100% solution)

RECOMMENDED USE % =0.2 to 0.4 %

REFERENCE: ECOLAB

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 26

P3-TOPAX 19 (ALKALINE CLEANING AGENT FOR FOAM CLEANING)

SL Test Parameters Specification

NO.
1. Appearance Clear yellowish liquid
It respond the test for sodium.
Identification Sodium: Acidify the solution with acetic acid (60 g/l). filter if
2.
necessary & treat with zinc acetate TS. A yellow crystalline ppt is
formed.
3. Density at 20°C 1.21 to 1.23 g/cm3
4. pH 12.4 to 12.6 (1 % solution at 20°C)
5. Conductivity 9.08 mS/cm (1 % solution at 25°C)
TA 3.7 to 4.3 ml of 0.5 N HCl is needed to find the end point of 50 ml of
6.
1% solution using phenolphthalein TS.
7. % Active 11.40 to 11.70 as NaOH

METHOD OF CALCULATING OF % ACTIVITY

1. Take 50 ml of sample.
2. Add phenolphthalein TS.
3. Titrate with 0.5 N HCl VS.

CALCULATION OF ACTIVITY PERCENTAGE

BR ×0.25= % ( by weight) Active as NaOH
0.25= Titration factor

RECMMENDED USE % = 2 to 5 % with contact time 10 to 15 minutes.

Precautions:
1. Standardize HCl Solution Before Titration.

2. Sample must be 50 ml.

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REFERENCE: ECOLAB

DRY TECH-1 (SILICONE BASED CONVEYOR LUBRICANT) FOR TETRAPAK

SL Test Parameters Specification

NO.
1. Appearance White milky liquid
2. Specific gravity at 20°C 1.01
3. pH 3.5 TO 4.0

DRY TECH-7(SILICONE FREE CONVEYOR LUBRICANT) FOR PET BEVERAGE

SL Test Parameters Specification

NO.
1. Appearance Pale yellow clear liquid
2. Specific gravity at 25°C 1.03-1.07
3. pH 4.0 -7.5

SU 321 (DETERGENT DISINFECTANT)

SL Test Parameters Specification

NO.
1. Appearance Clear, Colorless to Pale yellow liquid
2. Specific gravity at 25°C 1.0 to 1.1
3. pH 11.0 to 12.0 ( 1% solution)

REFERENCE: JohnsonDiversey

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P3-OXONIA ACTIVE 150 (ACID DISINFECTANT)

SL Test Parameters Specification

NO.
1. Appearance Clear colorless liquid with no particle
To a 1:1 mixture of ethyl acetate & peroxide solution, 1-2 drops of
potassium bicromate solution is added. When the solution is
2. Identification acidified with dil. H2SO4 , the aqueous layer becomes blue.when the
mixture is shaken & settled to separate, the blue color migrates to
ethyl acetate layer.
3. % H2O2 23.5 to 25.0
4. Density 1.13 g/cc
5. pH 2.8 (1%, 20°C)

METHOD OF H2O2 & PERACETIC ACID ANALYSIS

1. Take 1ml of sample & dilute it to 100 ml with water, then add 20 ml of 25% sulfuric acid.
2. Titrate with 0.1 N KMnO4 Solution until permanent pink color appeared.
3. Then add 5 ml of 10% KI Solution & 2 ml of 1% starch indicator & titrate with 0.1 N sodium thiosulfate until
colorless.

CALCULATION OF H2O2 PERCENTAGE

Each ml of 0.1 N KMnO4 is equivalent to 1.701 mg of H2O2.
BR of KMnO4 × Normality Of KMnO4 solution ×1.701
Sample Weight

CALCULATION OF PERACETIC ACID PERCENTAGE

Each ml of 0.1 N sodium thiosulfate is equivalent to 3.8 mg of PAA.
OR
BR for thiosulfate solution × Normality Of thiosulfate solution ×3.8
Sample Weight

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REFERENCE: ECOLAB

DIVOSAN FORTE VT6 (ACID DISINFECTANT)

SL Test Parameters Specification

NO.
1. Appearance Clear colorless liquid
To a 1:1 mixture of ethyl acetate & peroxide solution, 1-2 drops of
potassium bicromate solution is added. When the solution is
2. Identification acidified with dil. H2SO4 , the aqueous layer becomes blue.when the
mixture is shaken & settled to separate, the blue color migrates to
ethyl acetate layer.
2. Specific gravity at 20°C 1.15
pH (1% solution at 2.5 – 4.5 (1% solution at 25°C)
3.
25°C)

METHOD OF H2O2 & PERACETIC ACID ANALYSIS

1. Take 1ml of sample & dilute it to 100 ml with water, then add 20 ml of 25% sulfuric acid.
2. Titrate with 0.1 N KMnO4 Solution until permanent pink color appeared.
3. Then add 5 ml of 10% KI Solution & 2 ml of 1% starch indicator & titrate with 0.1 N sodium thiosulfate until
colorless.

CALCULATION OF H2O2 PERCENTAGE

Each ml of 0.1 N KMnO4 is equivalent to 1.701 mg of H2O2.
BR of KMnO4 × Normality Of KMnO4 solution ×1.701
Sample Weight

CALCULATION OF PERACETIC ACID PERCENTAGE

Each ml of 0.1 N sodium thiosulfate is equivalent to 3.8 mg of PAA.
OR
BR for thiosulfate solution × Normality Of thiosulfate solution ×3.8

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Sample Weight

Precautions:
1. Standardize KMnO4 & sodium thiosulfate & Solution before Titration.

REFERENCE: JohnsonDiversey

DICOLUBE RS 148 NEU

SL Test Parameters Specification

NO.
1. Appearance Clear, slightly yellow liquid
2. Specific gravity at 20°C 1.00
3. pH (1% solution) 6.1

SU 727 ( OXOFOAM) CHLORINATED FOAM CLEANER

SL Test Parameters Specification

NO.
1. Appearance Clear pale yellow liquid
2. Specific gravity at 20°C 1.21
3. pH (1% solution) 12.7

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REFERENCE: JohnsonDiversey

P3-LUBOKLAR EC (SOAP FREE CHAIN LUBRICANT FOR CONVEYORS)

SL Test Parameters Specification

NO.
1. Appearance Clear yellow liquid
2. Specific gravity 0.980 to 1.020
3. pH 6.40 to 6.80 (100% solution)

RECOMMENDED USE % =0.5 to 1.0 %

P3-STABICIP ABF (WETTING AGENT)

SL Test Parameters Specification

NO.
1. Appearance Clear colorless to slightly yellow liquid
2. Specific gravity 0.980 to 1.02
3. Refractive index 1.371 to 1.383

RECOMMENDED USE % =0.01 to 0.1 %

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REFERENCE: ECOLAB

P3-OXYPAK-D (LIQUID SANITIZER FOR PACKING MACHINE)

SL Test Parameters Specification

NO.
1. Appearance Clear, Colorless liquid
2. Specific gravity 1.11 to 1.15
3. pH 2.1 to 2.6
4. Concentration 33 to 37 % as hydrogen peroxide
METHOD OF ASSAY ANALYSIS
1. Accurately weighed about 1 ml of sample & dilute it to 100 ml with water.
2. Take 20 ml of stock solution & add20 ml of 2 N sulfuric acid.
3. Titrate with 0.1 N KMnO4 VS.

CALCULATION OF ASSAY PERCENTAGE

Each ml of 0.1 N KMnO4 is equivalent to 1.701 mg of H2O2
Or
BR × Normality Of KMnO4 solution ×1.701
Sample Weight

RECOMMENDED USE % = 100% & Contact time 4 to 8 seconds at 60 to 80°c.

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REFERENCE: ECOLAB

FOOD GRADE FLAVOR IN POWDER FORM (CHEESE PUFFS)

SL Test Parameters Specification

NO.
1. Appearance Orange colored powder
2. Moisture 1.7 %
3. NaCl 11.7 % to 12.7 %

REFERENCE: PERESSCOL SDN.BHD

FOOD GRADE FLAVOR IN POWDER FORM (TANGY TOMATO)

SL Test Parameters Specification

NO.
1. Appearance Fine powder with uniform distribution of spices
2. Moisture 6.00 % (max.)
3. NaCl 2.30 % to 4.30 %

REFERENCE: PERESSCOL SDN.BHD

CLEAR LEMON KIT PART-1

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SL Test Parameters Specification
NO.
1. Appearance Oily & Limpid liquid
2. Color Pale yellow to pale green
3. Specific gravity(d20/20) 1.032 to 1.052 at 20°c
4. Refractive index(nD/20) 1.429 to 1.439 at 20°c
Solution comparison 0.1 % (Beverage conc.) solution should be identical in TOA as
5.
compared with existing good one.

REFERENCE: FIRMENICH (SWITZERLAND)

TUTTI FRUITY FLAVOURING

SL Test Parameters Specification

NO.
1. Appearance Oily & Limpid liquid
2. Color Pale yellow
3. Specific gravity(d20/20) 1.021 to 1.041 at 20°c
4. Refractive index(nD/20) 1.431 to 1.441 at 20°c
Solution comparison 0.15 % (Beverage conc.) solution should be identical in TOA as
5.
compared with existing good one.

REFERENCE: FIRMENICH (SWITZERLAND)

COLA KIT PART-1

SL Test Parameters Specification

NO.
1. Appearance Liquid
2. Color Dark brown
3. Specific gravity(d20/20) 1.216 to 1.256 at 20°c

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Solution comparison 0.2 % (Beverage conc.) solution should be identical in TOA as
5.
compared with existing good one.

REFERENCE: FIRMENICH (BELGIUM)

MANGO FLAVOUR

SL Test Parameters Specification

NO.
1. Appearance Clear liquid
2. Color Colorless to light yellow
3. Specific gravity(d20/20) 1.0295 to 1.0395 at 20°c
4. Refractive index(nD/20) 1.4283 to 1.4383 at 20°c
Solution comparison 0.103 % (juce conc.) solution should be identical in TOA as
5.
compared with existing good one.

REFERENCE: SYMRISE

CLOUDY LEMON KIT PART-1

SL Test Parameters Specification

NO.
1. Appearance Viscous & cloudy liquid

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2. Color Cream
3. Specific gravity(d20/20) 1.057 to 1.077 at 20°c
Solution comparison 0.1 % (Beverage conc.) solution should be identical in TOA as
5.
compared with existing good one.

REFERENCE: FIRMENICH (BELGIUM)

ORANGE FLAVOR EMULSION E-204404

SL Test Parameters Specification

NO.
1. Appearance Emulsion
2. Color Whitish to pale yellow
3. Specific gravity(d20/20) 1.0520 to 1.0720 at 20°c
Solution comparison 0.16 % (Beverage conc.) solution should be identical in TOA as
4.
compared with existing good one.

REFERENCE: GIVAUDAN (SINGAPORE)

CEREAL HERBAL BEVERAGE CONCETRATE ( COMP MALT)

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SL Test Parameters Specification

NO.
1. Appearance Should be acceptable
2. °Brix 62.0 to 68.0
3. pH 3.30 to 4.30 at 20°c

REFERENCE: WILD (GERMANY)

MALT FLAVOUR

SL Test Parameters Specification

NO.
1. Appearance Should be acceptable
2. Color Pale yellow to pale green
3. Specific gravity(d20/20) 1.0040 to 1.06400 at 20°c
4. Refractive index(nD/20) 1.4130 to 1.4530
Solution comparison 0.036 % (Beverage conc.) solution should be identical in TOA as
5.
compared with existing good one.

REFERENCE: WILD (GERMANY)

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COMP GRAPE LEMON

SL Test Parameters Specification

NO.
1. Appearance Should be acceptable
2. Citric acid content 1.34 to 1.98 %
3. °Brix 53.7 to 55.7

REFERENCE: WILD (GERMANY)

GREEN MANGO FLAVOUR

SL Test Parameters Specification

NO.
1. Appearance Liquid
2. Color Colorless
3. Specific gravity(d20/20) 0.781 to 0.801 at 20°c
4. Refractive index(nD/20) 1.405 to 1.415 at 20°c

REFERENCE: FIRMENICH (INDONESIA)

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APPLE FLAVOURING

SL Test Parameters Specification

NO.
1. Appearance Should be acceptable
2. Specific gravity(d20/20) 1.09000 to 1.15000 at 20°c
3. Refractive index(nD/20) 1.4100 to 1.4500 at 20°c
Solution comparison 0.054 % (Beverage conc.) solution should be identical in TOA as
4.
compared with existing good one.

REFERENCE: WILD (GERMANY)

ROUNDING FLAVOUR

SL Test Parameters Specification

NO.
1. Appearance Should be acceptable
2. Specific gravity(d20/20) 0.92000 to 0.98000 at 20°c
3. Refractive index(nD/20) 1.4000 to 1.4400 at 20°c
Solution comparison 0.048 % (Beverage conc.) solution should be identical in TOA as
4.
compared with existing good one.

REFERENCE: WILD (GERMANY)

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 40

VITAMIN PREMIX HA3 F072000013

SL Test Parameters Specification

NO.
1. Appearance Fine granular powder
2. Color White
Solution comparison 0.068 % (Beverage conc.) solution should be identical in TOA as
3.
compared with existing good one.

REFERENCE: DSM (SINGAPORE)

SUNSET YELLOW

SL Test Parameters Specification

NO.
1. Appearance Orange yellow powder
2. Moisture 11.0 %
Solution comparison 0.0015 % (Beverage conc.) solution should be identical as compared
3.
with existing good one.

TARTRAZINE

SL Test Parameters Specification

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NO.
1. Appearance Lemon yellow powder
2. Moisture 11.5 %
Solution comparison 0.0006 % (Beverage conc.) solution should be identical as compared
3.
with existing good one.

REFERENCE: ROHA, INDIA

IDACOL SUNSET YELLOW

SL Test Parameters Specification

NO.
1. Appearance Orange yellow powder
2. Moisture 11.2 %
Solution comparison 0.0015 % (Beverage conc.) solution should be identical as compared
3.
with existing good one.

IDACOL TARTRAZINE

SL Test Parameters Specification

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NO.
1. Appearance Lemon yellow powder
2. Moisture 10.5 %
Solution comparison 0.0006 % (Beverage conc.) solution should be identical as compared
3.
with existing good one.

IDACOL CARMOISINE

SL Test Parameters Specification

NO.
1. Appearance Red to maroon powder
2. Moisture 9.45 %
Solution comparison 0.00015 % (Beverage conc.) solution should be identical as
3.
compared with existing good one.

REFERENCE: ROHA, INDIA

BETA CAROTENE

SL Test Parameters Specification

NO.
1. Appearance Red granular powder
2. Moisture 3.19 %
Solution comparison 0.018 % (fruit drink conc.) solution should be identical as compared
3.
with existing good one.

REFERENCE: INHOUSE

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ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 43

GRINDSTED XANTHAN GUM 200

SL Test Parameters Specification

NO.
1. Appearance
2. Moisture 7.5 to 9.3
3. Viscosity (0.8%solution) 1300 to 1380 Cp, 60rpm, spindle L3
4. pH 7.0 to 7.1 (1% solution)

REFERENCE: DANISCO

MICRO CRYSTALLINE CELLULOSE POWDER

SL Test Parameters Specification

NO.
1. Physical State White powder
2. pH 5.5 to 7.0 ( 1% solution)
3. Moisture (%) Maximum 7.0 %
4. Bulk density 0.26 to 0.34 g/cc

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REFERENCE: Mingtai Chemical co. ltd ( Taiwan)

CORN GRIT

SL Test Parameters Specification

NO.
1. Physical State Pale yellow granular powder
2. Particle size 425 to 1000 micron
3. Moisture (%) 10 to 11 %
4. Black particle Nil

REFERENCE: In house

MANGO PULP( ALLANA)

SL Test Parameters Specification

NO.
1. Appearance Uniform, homogenous consistency, free from fibers.
2. Color Bright yellow
3. Flavor & taste Typical of ripe fruits, free from any off flavor.
4. Acidity (%) 0.80 maximum
5. °Brix at 20°c Not less than 14.0

Name Designation Sign & Date

Prepared By
Reviewed By S.M. Ayenul Islam Manager, QA
Authorized By
 MEGHNA BEVERAGE LIMITED.
TOPORDI, SONARGOAN,nARAYANGONJ

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 45

6. pH at 20°c 3.40 to 4.20
Black or brown Maximum 10
7.
specks/10 g

REFERENCE: Frigerio conserva allana limited.

MANGO PULP( GALLA)

SL Test Parameters Specification

NO.
1. Appearance Uniform texture, smooth
2. Color Golden yellow
3. Flavor & taste Characteristic natural ripe totapuri mango
4. Acidity (%) Minimum 0.40
5. °Brix at 20°c Not less than 14.0
6. pH at 20°c 3.6 to 4.00
Black or brown Maximum 10
7.
specks/10 g

Name Designation Sign & Date

Prepared By
Reviewed By S.M. Ayenul Islam Manager, QA
Authorized By
 MEGHNA BEVERAGE LIMITED.
TOPORDI, SONARGOAN,nARAYANGONJ

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 46

IDENTIFICATION OF DEXTROSE

ADD FEW DROPS OF DEXTROSE SOLUTION (1 in 20 ml of water) IN HOT ALKALINE CUPRIC TARTRATE SOLUTION.
RED PPT IS APPEARED.

PREPARATION OF CUPRIC TARTRATE SOLUTION:

1. 34.66 g OF CUPRIC SULFATE DISSOLVE IN 500 ML OF WATER.

2. 173 g OF SODIUM POTTASIUM TARTRATE DISSSOLVE IN 500 ML OF WATER & ADD 50 g OF NaOH.
Mix the solution 1 & 2 in equal volume just before test.

IDENTIFICATION OF ACETIC ACID

Add FeCl3 Neutral solution to neutral solution of acetic acid. Red ppt is formed.

Name Designation Sign & Date

Prepared By
Reviewed By S.M. Ayenul Islam Manager, QA
Authorized By
 MEGHNA BEVERAGE LIMITED.
TOPORDI, SONARGOAN,nARAYANGONJ

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 47

Preparation of neutral solution of FeCl3 solution:

Take about1 ml of ferric chloride solution. Add ammonium hydroxide solution until ppt is formed. Add
dil hydrochloric acid till ppt dissolved.

P3-STABICIP ABF
Molecular Formula: Formula weight: Equivalent Weight:

SL Test Parameters Specification

NO.
1. Physical State
2. Specific gravity
3. Purity (%)

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

Name Designation Sign & Date

Prepared By
Reviewed By S.M. Ayenul Islam Manager, QA
Authorized By
 MEGHNA BEVERAGE LIMITED.
TOPORDI, SONARGOAN,nARAYANGONJ

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 48

Precautions:
1. Standardize KMnO4 Solution Before Titration.

2. Weighing Should Be Accurate.

Name Designation Sign & Date

Prepared By
Reviewed By S.M. Ayenul Islam Manager, QA
Authorized By