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KT 100355

KREATIVE TECHNOLABS USA

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Kreative Technolabs
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57 views2 pages

KT 100355

KREATIVE TECHNOLABS USA

Uploaded by

Kreative Technolabs
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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DATA SHEET
Brilliant Violet 785™ anti-mouse CD3ε Antibody

Catalog# / Size 100355 / 50 µg

Clone 145-2C11

Other Names CD3ε, T3, CD3

Isotype Armenian Hamster IgG

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-
T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ
chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by
binding the peptide/MHC antigen complex.

Product Details

Reactivity Mouse

Antibody Type Monoclonal

Host Species Armenian Hamster

Immunogen H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under
optimal conditions.

Concentration 0.2 mg/ml

Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged
exposure to light. Do not freeze.

Application FC - Quality tested

Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with Flow cytometric analysis.
For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It
is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter
optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software
setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a
trademark of Sirigen Group Ltd.

Excitation Laser Violet Laser (405 nm)

Application Notes
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing
proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, and in vivo T cell depletion8-10.
Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of
acetone- fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in
vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported
to block the binding of 17A2 antibody to CD3 epsilon- specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin
embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free,

0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-
LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Ste# 619, 606 S Hill Street,Los Angeles, CA 90014, Phone: +1 (213) 596-8884, Email: [email protected] 
 

Antigen Details

Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD

Distribution Thymocytes (differentiation dependent), mature T cells, NK-T cells


Function TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor Peptide antigen/MHC-complex
Cell Type NKT cells, T cells, Thymocytes, Tregs

Biology Area Immunology

Molecular Family CD Molecules, TCRs

Gene ID 12501

Product Data

C57BL/6 mouse splenocytes were stained


with CD3ε (clone 145-2C11) Brilliant Violet
785™ (filled histogram) or Armenian
hamster IgG Brilliant Violet 785™ isotype
control (open histogram).

For In-Vitro Diagnostic Use. Not For Human Or Animal Consumption.

*These products may be covered by one or more Limited Use Label Licenses (see the Kreative Catalog or our website,
www.kreativetechnolabs.com/ordering#license). Kreative products may not be transferred to third parties, resold, modified for resale, or
used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without
written approval of Kreative. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.

Ste# 619, 606 S Hill Street,Los Angeles, CA 90014, Phone: +1 (213) 596-8884, Email: [email protected] 

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