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Pre-Analytical Factors of Platelet: Counting

This document discusses pre-analytical factors that can affect platelet counting. It notes that EDTA is the recommended anticoagulant as it causes the least changes to blood cells. However, EDTA can sometimes cause pseudothrombocytopenia where autoantibodies cause platelets to agglutinate, appearing as a low platelet count but not reflecting the actual in vivo level. Other pre-analytical issues like poor blood collection technique can also result in artificially low platelet counts. Using anticoagulant mixtures containing calcium or magnesium have been shown to prevent EDTA-induced platelet agglutination.

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34 views

Pre-Analytical Factors of Platelet: Counting

This document discusses pre-analytical factors that can affect platelet counting. It notes that EDTA is the recommended anticoagulant as it causes the least changes to blood cells. However, EDTA can sometimes cause pseudothrombocytopenia where autoantibodies cause platelets to agglutinate, appearing as a low platelet count but not reflecting the actual in vivo level. Other pre-analytical issues like poor blood collection technique can also result in artificially low platelet counts. Using anticoagulant mixtures containing calcium or magnesium have been shown to prevent EDTA-induced platelet agglutination.

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Iberis
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© © All Rights Reserved
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Section 3

PRE-ANALYTICAL
FACTORS OF PLATELET
COUNTING

11
PLATELET COUNTING LEARNING GUIDE
DIAGNOSTICS
PRE-ANALYTICAL FACTORS OF PLATELET COUNTING

Choice of anticoagulant
Like all blood cells, platelets are analyzed in blood that is made non-clottable with an anticoagulant. In regular
laboratory practice the recommended anticoagulant for hematologic investigations is the dipotassium salt of
EDTA (K2 -EDTA), as this causes the least changes in blood cells.9 It is known that the platelet count remains
stable in EDTA blood, but mean platelet volume (MPV) does not.10,11 In general, MPV increases over time after
blood draw, although this may depend on the technology used in the analyzer.12 Recently it was found that even
the brand of K2-EDTA is a factor that can cause clinically significant pre-analytical variation.10 Furthermore, it is
important to realize that the quality of blood collection tubes is not always in agreement with specified criteria.14

Anticoagulants other than EDTA are not recommended for cellular analysis, however citrate-based anticoagulants
may be a preferred option for special studies on MPV, because citrate does not affect MPV after blood draw.15,16

EDTA-induced pseudothrombocytopenia
Spuriously low platelet counts due to EDTA-induced platelet agglutination is frequently observed in any medical
laboratory; the incidence ranges from 0.07–0.20 % in unselected donors to as high as 2.0 % in hospitalized patients
(1, 14–16). There is no apparent association with disease, although some reports suggest that this phenomenon is
more frequent in autoimmune diseases and hepatitis A virus infection.20

Classically, EDTA-induced platelet agglutination is caused by auto-antibodies, which normally do not react with
platelets. When blood comes in contact with EDTA, a chemical that binds calcium ions, the spatial structure of the
platelet surface proteins is altered. As a result, these surface proteins now become accessible to the auto antibodies
causing the platelets to stick together in large agglutinates, thereby reducing the number of free platelets in
the blood sample (pseudothrombocytopenia). EDTA agglutination is purely an in vitro phenomenon, and the
antibodies have no in vivo effect.21–23 Some authors have reported that EDTA-induced thrombocytopenia may also
be dependent on temperature and time.24

Recently it was recognized that EDTA-thrombocytopenia is not limited to auto-antibodies, as several cases
have been reported where therapeutic monoclonal anti-platelet antibodies were involved.25–27 In addition, it was
postulated that not only EDTA, but also hirudin caused anticoagulant-induced pseudothrombocytopenia.28

A closely related phenomenon, supposedly due to the same mechanism but much less frequent, is formation of
platelet-neutrophil agglutinates or platelet satellitism.1, 29–31 Even rarer is satellitism of platelets around malignant
lymphoma cells.32 This can lead to the reduction in platelets counts.
Alternatively, anticoagulant mixtures containing calcium or magnesium have been demonstrated to effectively
prevent EDTA-induced platelet agglutination.35,36 This means that it is no longer necessary to collect another blood
sample in an alternative anticoagulant, as is still common practice in many laboratories.

Other pre-analytical factors


Apart from platelet agglutination, poor blood collection technique is another frequent source of falsely low platelet
counts, irrespective of the measuring technology. After prolonged stasis or when blood is not properly mixed with
anticoagulant during specimen drawing, the blood coagulation system can become activated and small clots may
form, consequently resulting in spuriously low platelet counts.37

12

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