This study evaluated the growth and reproductive responses of bush beans inoculated with rhizosphere soil and root nodules from Centrosema pubescens. Seeds were planted in pots with sterilized soil and inoculated with a mixture of rhizosphere soil and root nodules from C. pubescens or a negative/positive control. Inoculation hastened later stage plant growth and increased early leaf number but had no effect on height, leaf size, or reproduction. Inoculation also increased root biomass and formation of root nodules on the bush bean plants.
This study evaluated the growth and reproductive responses of bush beans inoculated with rhizosphere soil and root nodules from Centrosema pubescens. Seeds were planted in pots with sterilized soil and inoculated with a mixture of rhizosphere soil and root nodules from C. pubescens or a negative/positive control. Inoculation hastened later stage plant growth and increased early leaf number but had no effect on height, leaf size, or reproduction. Inoculation also increased root biomass and formation of root nodules on the bush bean plants.
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ABSTRACT
BOADO, JAMAICA D., DACANAY, MICHAEL CHRISTIAN T.,
ESPERANZA, LOUISE ISABELLE M., GATCHALLAN, MARY ANGEIRINA P., OSILLOS, EEVAN GELL L. (2019). Growth and Reproductive Responses of Bush Beans (Sumilang) to Rhizosphere Soil and Root Nodules Associated with Centrosema pubescens BENTH. (Sam-samping). Science, Technology, Engineering and Mathematics (STEM). Don Mariano Marcos Memorial State University South La Union Campus, Laboratory High School, Agoo, La Union.
Adviser: Flores Jr., Ernesto G.
The rhizosphere soil is a rich source of growth-promoting rhizobacteria that
maintains plant health. Wild legumes that are considered as useless can be hosts of rhizobacteria. One noticeable legume found in the wild is Centrosema pubescens Benth. or sam-samping. The rhizosphere soil (RS) and root nodule (RN) of this plant can be a potential source of inoculant. A pot experiment was therefore conducted to evaluate the growth and reproductive responses of bush beans to RS and RN inoculation measured in terms of plant height, percent increase in height, number of leaves, leaf size, number of flowers, and days to flowering. It also determined the effect of the inoculants to the belowground productivity and root nodule formation in the bush bean roots. Seeds of bush beans were sown in pots containing 10 kg sterilized soil. Two weeks after germination, the following treatments namely, Negative control , Positive control, and those inoculated with a mixture of RS and RN associated with C. pubescens . The treatments were replicated three times with three pots used per replicate. Results showed that inoculation of RS and RN associated with C. pubescens hastened the growth of the plants on the later stage of growth phase, and effective in increasing the number of leaves during the early stages of growth but not on the later stage. However, it has no effect on the height, leaf size and to the reproductive performance of the plants. Inoculation also leads to a higher root biomass and formation of root nodules.