ABG Sampling (10-15 SOLANO) .Odt
ABG Sampling (10-15 SOLANO) .Odt
10-15
ABG Sampling
Indications:
Blood sampling in infants for laboratory studies unaffected by hemolysis.
Arterial blood gas analysis for evaluating the respiratory status and acid-base equilibrium in patients with
respiratory distress or metabolic derangements.
For routine laboratory analysis or blood culture if venous blood is difficult to obtain.
Contraindications:
Negative modified Allen Test
-Denotes presence of ulnar artery occlusion.
Any inflammation, infection, burns, or poor integrity at selected puncture site
Bleeding problems or coagulopathy
2. Disinfect the puncture site following an inward to outward circular motion for 30 seconds then allow it to air dry.
3. Wash hands or disinfect hands with alcohol.
4. Don gloves.
5. Prepare and administer local anesthetic subcutaneously over the planned puncture site (making sure to aspirate
to ensure you are not within a blood vessel prior to administration). This is optional.
6. Expel the heparin through the needle.
7. Insert needle or puncture the pulse at 30-45 degree angle over the point of maximal impulse.
- Neonates have arteries that are more shallowly located. Therefore, the needle is inserted at a lesser angle
(ie. More parallel to the skin).
1. Observe blood flashback. Blood should flow freely into the syringe in a pulsatile fashion.
2. Allow syringe to fill to the appropriate level.
3. Once the sample is obtained, remove the needle and apply immediate firm, constant pressure for 5 minutes and
then place a pressure dressing on the puncture site.
Note: Do not infuse any medications, blood products, or hypotonic solutions through an arterial line.
5. Remove needle.
9. Wash hands.
10. Thank the patient.
11. Send specimen to the laboratory immediately.
Complications:
Infection
Bleeding
Occlusion of artery by hematoma or thrombosis
Ischemia if ulnar circulation is inadequate
Post-procedure pain
Sources:
1. UST Guide for history taking and physical examination and diagnosis of pediatric patients handbook (3 rd Edition)
2. https://www.utmb.edu/policies_and_procedures/Non-
IHOP/Respiratory/Respiratory_Care_Services/07.03.40%20Neonatal%20Pediatric%20Arterial%20Puncture.pdf
3. Youtube: https://m.youtube.com/watch?v=0BSv4iN8T2E&t=31s