Scribd
Upload
31 views

Counting Cells Workshop August, 2015: Notice The Boundary Lines

The document outlines the steps of a counting cells workshop which includes: 1) Getting familiar with using a microscope by observing prepared slides at different magnifications. 2) Preparing a yeast suspension slide. 3) Learning to identify the grids and sample squares on a haemocytometer under the microscope at different magnifications in order to count cells. 4) Practicing loading samples into the haemocytometer. 5) Loading and observing a yeast suspension sample under the microscope. 6) Systematically counting the cells within the sample squares and those touching boundary lines in 5 squares. 7) Estimating the total number of cells from the counts.

Uploaded by

Zico
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
31 views

Counting Cells Workshop August, 2015: Notice The Boundary Lines

The document outlines the steps of a counting cells workshop which includes: 1) Getting familiar with using a microscope by observing prepared slides at different magnifications. 2) Preparing a yeast suspension slide. 3) Learning to identify the grids and sample squares on a haemocytometer under the microscope at different magnifications in order to count cells. 4) Practicing loading samples into the haemocytometer. 5) Loading and observing a yeast suspension sample under the microscope. 6) Systematically counting the cells within the sample squares and those touching boundary lines in 5 squares. 7) Estimating the total number of cells from the counts.

Uploaded by

Zico
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 3

Counting cells workshop August, 2015

1. Getting to know the microscope:


 “Finding your way round the microscope”
 Observe a prepared slide – adjust light, focus at x40, x100 and x400.
 Practise moving the slide around

2. Prepare a slide of yeast suspension.


 Observe at x40, x100, x400

3. “Finding your way round the haemocytometer”


 Identify sample injection area

 Look at grids in the detection area with the


naked eye
 Observe one detection area at x40 and identify the central grid

 Observe at x100 and identify ‘sample’ squares

 At x400, find the top left and right, central and bottom left and right sample squares.

Notice the boundary lines

1
 Practise moving the haemocytometer around to find the sample squares.
4. Use a coloured dye to practise loading the haemocytometer using a capillary tube (load one
sample injection area).

5. Load the other sample injection area of the haemocytometer with yeast suspension
 Observe at x40, x100 and x400
 Check that you can find the sample squares at x400

6. Counting the cells


 Count systematically

 Count all the cells within the sample square and those
touching the top and left middle boundary lines

 Count the cells in all 5 sample squares

Square 1 ___________ Square 2 ___________

Square 3 ___________

Square 4 ___________ Square 5 ___________

2
7. Estimating the number of cells

You might also like