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Existing Open Source Bioreactors

This document describes several open source bioreactor designs that can be built for culturing microorganisms or cells. The designs range in size from 10 ml to 20 L and cost between $400-$2250. Each design includes features like temperature control, agitation, media supply, and sensors for measurements like optical density, pH, and weight. Construction manuals, code, design files, articles, and wiki pages are provided as resources to build the different bioreactor designs.

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Vidushee Geetam
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69 views

Existing Open Source Bioreactors

This document describes several open source bioreactor designs that can be built for culturing microorganisms or cells. The designs range in size from 10 ml to 20 L and cost between $400-$2250. Each design includes features like temperature control, agitation, media supply, and sensors for measurements like optical density, pH, and weight. Construction manuals, code, design files, articles, and wiki pages are provided as resources to build the different bioreactor designs.

Uploaded by

Vidushee Geetam
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Open Source Bioreactors

Bioreactors provide a controlled environment for culturing microorganisms or cells.


Conditions that can be optimized for cell growth are:
● Medium in which the cells are grown
● Temperature
● Oxygen, Nitrogen
● Agitation
● pH

This is the start of a list of open source designs to build one for the Biomaker Challenge
2018 and the Open Bioeconomy project.

iGEM Aachen 2015

Features
● DIY peristaltic pumps with a dynamic range of 0.5-1400 ml/h
● semi-continuous OD measurement unit
● calibrated magnetic stirring from 100-1400 rpm
● integrated circuit for controlling three pumps and stirring
● designed DIY constructions to hold circuit, pumps and bioreactor setup
● parallelization of multiple reactor control units

Resources
● Construction Manuals

Cost: £400 for three


Size: 10 ml per vessel

Hackaurium open source bioreactor

Features
● Fully automated bioreactor
● Semi batch operation using 2 pumps
● Weight sensor
● Temperature control
● 2 valves for pH regulation using HCl and NaOH
● Constant pressure in the reactor
● Anemometer to measure gas flux under 1L/min
● Storage of parameters
● Remote control
Resources
● Github Code and Design Files
● V3 Manual
● Presentation

Cost: $1790.0
Size: 1 L

Aeronaut Brewing Company Bioreactor


Features
● Made with readily available low cost components
● Autoclavable and sized to fit inside laminar flow hood in order
● to maintain an aseptic environment
● Interface sensor and control functions for:
○ Agitation
○ Media Supply
○ Temperature Control
○ Optical Density Sensor Loop

Resources
● Website

Cost: $450 (electronics only)


Size: 8 L

An automated home-built low-cost fermenter suitable for large-


scale bacterial expression of proteins in Escherichia coli

Features
● Made with readily available low cost components
● Autoclavable and sized to fit inside laminar flow hood in order
● Agitation
● Media Supply
● pH Control
● Temperature Control
● Optical Density Sensor Loop

Resources
● Article

Cost: $2250
Size: 12 L
Versatile Continuous Culture Device (VCCD)

Features
● Made with readily available low cost components
● Agitation
● Media Supply
● pH Control
● Temperature Control
● Optical Density Sensor Loop
● Fluorometer

Resources
● Article with all design files

Cost: ~$1,400
Size: 20 ml

Klavins Lab Chemostat

Features
● Made with readily available low cost components
● Agitation
● Media Supply
● Optical Density Sensor Loop

Resources
● Publication
● Wiki
● Parts list
● Github for files and designs

Cost: $2000 USD


Size: 15 ml

Other ideas to consider


● Bubble aeration using an aquarium pump
● Kalman filter for measuring growth rates
● MiniStat is work in progress
● Optical sensors using agarose film-coated glass slides

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