S p e c t ro L i g h t 6 0 0™

S p e c t ro L i g h t 6 0 0
™

IN-PLATE DYNAMIC LIGHT SCATTERING

UV AND WHITE LIGHT IMAGER
for hanging drops, sitting drops and
other microvolume applications IN-PLATE DYNAMIC LIGHT SCATTERING,
UV AND WHITE LIGHT IMAGER
ANALYSE, MONITOR AND IMAGE SUB μl DROPLETS
SpectroLight 600/610 a fully automated non-invasive in-drop DLS and UV
imaging system designed to operate on standard plates to perform high
throughput DLS measurements in sub-microliter volumes. Allowing single
OTHER MEMBERS OF THE SYSTEM FAMILY or serial measurements on a variety of conditions in parallel.
With SpectroLight 600/610 you can yield the maximum information for
characterisation of biomolecules.
ü Quickly screen sample stability and quality
- aging, oxidation, temperature denaturation, chemical degradation
ü Assess homogeneity and assembly state
ü Optimise buffer conditions, e.g. by selection of a specific detergent for
membrane proteins solubility
ü Investigate protein interactions, e.g. oligomerisation, aggregation,
denaturation, receptor-ligand interactions
ü Track each drop‘s progress towards crystallisation in-situ and non
S p e c t ro S i z e 3 0 0
™
invasively
Dynamic Light Scattering in cuvetts ü See nucleation long before crystals appear
for protein quality control ü Rapidly record UV and white light images
ü No cleaning required

XtalLight 200/210 ™

High resolution imaging system with

optional intrinsic fluorescence imaging

XtalLight 100 ™
Automated white light and optional intrinsic
Distinction of lysozyme crystals and NaCl crys- Easy access to all stored data by an integrated
Combined UV and white light source, tals when illuminated with white light (left) and fluorescence imaging (drop size 400 nl, robot
laboratory information management system (LIMS), loaded), optical resolution 2 µm.
adaptable to optical microscopes and UV light (right) that excites intrinsic blue protein recording and cross referencing all data. DLS laser is automatically positioned into the
imaging systems fluorescence.
sample drop.

www.xtal-concepts.de www.xtal-concepts.de
 S p e c t ro L i g h t 6 0 0™
TECHNICAL DATA S p e c t ro L i g h t 6 0 0
™

Laser diode ü Wavelength: 660 nm, optical power: 100 mW, adjustable
 other wavelengths
APPLICATIONS OF IN PLATE DLS ON SMALLEST SAMPLE VOLUMES ~ 400 - 800 nl
Detector ü Photomultiplier tube, dark count rate < 300 Hz
quantum efficiency 5-7%, count sensitivity 1.5*105 Hz/pW
ü For single photon counting
ü Scattering angle 142°
 Avalanche photodiode, higher sensitivity for wavelengths > 660 nm

Correlator ü Multi-tau architecture correlator to cover a wide sample time range

ü Sample time from 400 ns to 30 s
ü Total 208 channels, quasi logarithmic channel spacing
Sensitivity ü Sample concentration with standard laser (660 nm)
ü Minimum 0.3 mg/ml at 2 µl for ~30 kDa proteins and 2.0 mg/ml for ~14 kDa
proteins (e.g. for lysozyme)
ü Maximum > 100 mg/ml
SpectroLight 600/610 is designed to use plates in Easy selection and comparison of DLS measurements
SBS format and some other plates like Terasaki or Imaging system ü Built-in microscope
Cellstar. 5 magnification steps: 0.63, 1.25, 2.0, 3.2, 6.4
Field of view: 10.5x7.6, 5.2x2.9, 3.3x2.5, 2.0x1.5, 1.0x0.75 mm
Resolution: 25 µm, 13 µm, 8 µm, 5 µm, 2.5 µm per pixel
Monitoring of size changes of a viral polyprotein ü CCD colour camera 1600 x 1200 pixels
in presence of its specific protease. Each curve
represents a series of in situ DLS measurements.
 other resolutions

Illumination ü White light integrated LED
 UV by external light source
Operation temperature ü Built-in temperature control
ü Range 4 to 40°C (at ambient temperature 20°C)
Sample properties ü Minimum droplet volume about 0.5 µL
ü Particle sizes from 1nm to approx. 6 µm
Sample container ü Plates in SBS format
Sitting drop: e. g. MRC 96 well, Maxiplate 48 well,
Hanging drop: Cellstar
Others : Costar 3590, LCP plate
ü Terasaki microbatch plates (with adapter)
 customised sample holder
Dimensions ü Table top system 520 mm x 230 mm x 450 mm (LxWxH)
ü Weight: approx. 22 kg
ü Power consumption: 115 to 230 V, 100 W
Examples for studying protein stability, oxidation of Buffer adaptation in terms of a specific detergent for
a lectin (left), degradation of a viral protein (right) membrane protein requirements. Depending on Software features ü SpectroLight 600 software runs on Linux
detergent, the hydrodynamic radius changes from ü Fully automated plate scanning with unique drop finding algorithm
6.63 +/-0.06 nm to 2.93 +/-0.08 nm ü Integrated LIMS database for storage and retrieval of images and DLS
data
ü Control of light source parameters
ü Live display of camera image
ü Graphical histogramming software
Bacterial surface layer protein stability ü Radius distribution 2D and 3D
study in presence of bivalent cations
 connection to external data base
Ca2+ and Mg2+
 connection to plate handling system