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UNIT 2 MODULE 2 Spectro...

The document discusses analytical methods and separation techniques, specifically spectroscopic methods of analysis including ultraviolet-visible spectroscopy. It outlines objectives and explanatory notes for students including describing the basic steps involved in UV-VIS spectroscopy and using Beer-Lambert's Law to calculate concentrations.

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Shaquille Dunkley
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20 views

UNIT 2 MODULE 2 Spectro...

The document discusses analytical methods and separation techniques, specifically spectroscopic methods of analysis including ultraviolet-visible spectroscopy. It outlines objectives and explanatory notes for students including describing the basic steps involved in UV-VIS spectroscopy and using Beer-Lambert's Law to calculate concentrations.

Uploaded by

Shaquille Dunkley
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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UNIT 2 MODULE 2: ANALYTICAL METHODS AND SEPARATION TECHNIQUES (cont’d)

SPECIFIC OBJECTIVES EXPLANATORY NOTES SUGGESTED PRACTICAL ACTIVITIES

Spectroscopic Methods of Analysis cont’d

Students should be able to:

4.2. state the approximate wavelength ranges of the X-ray, UV/VIS, IR and radiofrequency regions of the
electromagnetic spectrum; and,

Relative energies and dangers associated with exposure to high energy wavelengths.

Insert actual ranges for xray, UV, VIS, IR and RF.

4.3. recall that the energy levels in atoms and molecules are quantised.

Refer to Unit 1, Module 1.

5. Ultra Violet-Visible (UV/VIS) Spectroscopy

Students should be able to:

5.1. explain the origin of absorption in UV/VIS spectroscopy;

Simple treatment based on Unit 1, Module 1. Consideration of sigma (σ), pi (π), anti-bonding (σ*, π*)
and non-bonding (n) orbitals.
5.2. explain why some species will absorb light in the UV/VIS region whereas others will not;

5.3. describe the basic steps involved in analysing samples by UV/VIS spectroscopy;

Brief mention should be made of the use of complexing reagents to form coloured compounds.
Sensitivity and detection limits.

5.4. use Beer-Lambert’s Law to calculate the concentration of a given species in solution; and,

Use of standards and calibration curves.

5.5. list examples of the use of UV/VIS spectroscopy in the quantitation of substances.

Iron tablets; glucose and urea in blood; cyanide in water

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