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Brief Report Experiment 1

1) The document describes an experiment to calibrate a 25-mL pipette by weighing distilled water dispensed from the pipette. 2) The volume of the pipette was calculated to be 24.96 mL based on the mass of water dispensed and water's density, with an accuracy of 99.84% compared to the theoretical 25 mL volume. 3) Sources of error that could account for the small inaccuracy include systemic error, random error, touching the pipette tip to container sides during dispensing, and errors in weighing. Proper pipetting technique is important to minimize errors.

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69 views4 pages

Brief Report Experiment 1

1) The document describes an experiment to calibrate a 25-mL pipette by weighing distilled water dispensed from the pipette. 2) The volume of the pipette was calculated to be 24.96 mL based on the mass of water dispensed and water's density, with an accuracy of 99.84% compared to the theoretical 25 mL volume. 3) Sources of error that could account for the small inaccuracy include systemic error, random error, touching the pipette tip to container sides during dispensing, and errors in weighing. Proper pipetting technique is important to minimize errors.

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nuranisss 1
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EXPERIMENT 1

TITLE : CALIBRATION OF A 25-ML PIPETTE


NAME : NUR ANIS BINTI OTHMAN
MATRIC NO : U2004643
CLASS : M1200H
COURSE : SIC1002
LECTURE’S NAME : DR NOR ASRINA SAIRI
Brief Report Experiment 1

Title : CALIBRATION OF A 25-ML PIPETTE

Objectives :

1) Learning to calibrate a 25-mL pipette before any volumetric analysis is carried out.

2) Determine the accuracy of pipette

3) Develop a proper techniques for calibrating pipette

Introduction

A pipette is used to precisely measure and transfer small volumes of liquid in the laboratory in
experiments that demand high accuracy. Therefore, necessary to check pipette calibration
Before any volumetric analysis to ensure accuracy by dispensing right volumes. The error in
graduation mark may exceed the error allowed in measurement.

The pipette is calibrated by weighing distilled water in it at room temperature, and then
calculating the volume from the weight of water in air. A correction for the buoyancy of air is
included. The formula allows for the determination of the weight in vacuum, W after
correction, where Wa is the weight in air. The volume is calculated from the weight and the
density.

W = Wa + 1.06 Wa/1000

Method

The weight of the weighing bottle and its cover is take.

Transfer distilled water in a 25-mL pipette into the weighing bottle.

The bottle is weigh and the weighing is repeat until two consecutive readings do not differ by
more than 0.005 g

Record the room temperature

Calculate the volume of the pipette


Calculation for set 3 data

Average weight (g) = (24.8521 + 24.8536 + 24.8516 + 24.8503) / 4

= 24.8519 g

W = Wa + 1.06 Wa/1000

W = 24.8519 g + 1.06(24.8519)/1000

= 24.8782 g

Volume = Mass of distilled water/Density of water

= 24.8782/0.99654

= 24.96 mL

Percentage Accuracy= (Exp/theory) * 100

= (24.96/25.00) * 100

= 99.84%

Discussion

The volume of pipette can be determined by using a few steps. Firstly, by calculate average
weight of water from the data and using formula W = Wa + 1.06 Wa/1000 to obtain weight in
vacuum. Lastly using formula Volume of pipette= mass of distilled water/ density of water to
determine the volume of pipette.

The volume of pipette obtained is 24.96 mL in this experiment while the theoretical volume of
pipette is 25 mL and the accuracy in this experiment is 99.84%. Therefore, the result is not
accurate to the theoretical volume but precise. This might be either due to systemic error or
random error. In the experiment, some error such as touching the pipette tip to the container
sides and error while weighing the mass of water will affect the expected result. Another error
is parallax error: observer’s eye not the same level with the volume mark and the pipet vertical.

In order to overcome the errors, we have to follow all the precautions and correct techniques
when handling the pipette, to get accurate result.

Conclusion

From this experiment, it can be concluded that before any volumetric analysis is carried out the
pipette must be calibrated. Next, I am able to handle pipette correctly using proper techniques
to avoid errors in the experiment. Furthermore, able to understand accuracy of pipettes in the
laboratory by relating pipetted volume to mass measurements.
Questions and answer

Does the pipette empty from full volume to zero or from zero to full volume?

- The pipette empty from full volume to zero

Is the pipette designed to be emptied by gravity with the tip in contact with the vessel or to be
expelled by blowing out with pipette filler? Explain.

- The pipette is designed to be emptied by gravity with the tip in contact with the vessel
because it is not forced during the delivery.

10 correct techniques in handling pipette.

1. Make sure the pipette tip is not broken.


2. Ensure there is no droplets in pipette before proceed to the experiment.
3. Handle pipette at temperature equilibrium.
4. Examine the pipette tip before dispensing sample.
5. Pull the pipette straight out from the sample container and ensure not touch the container’s
wall.
6. Immerse the pipette tip in proper depth not too shallow and not too deep in the solution.
7. Before use the pipette, rinse pipette with distilled water 2 or 3 times.
8. Do not blow or force out whatever remains in the tip.
9. Ensure that the pipette tip is always above the level of the solution in the receiver during
delivery.
10 . Always hold pipette vertically when drawing solution in.
11. When dispensing the liquid, hold the pipette vertically but keep the sidewall of the
receiving vessel at 45 degrees.

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