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ORD CD AMU Notes

The document discusses optical rotatory dispersion (ORD), which is the measurement of how the optical rotation of chiral molecules changes with wavelength. It covers the fundamental principles of ORD, instrumentation, the Cotton effect phenomenon near absorption bands, and applications of ORD such as determining absolute configuration and conformations. Specific topics include circular dichroism, polarimetry, and ORD applications in quantitative analysis, stereochemistry, and equilibrium studies.

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Til Sen
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259 views

ORD CD AMU Notes

The document discusses optical rotatory dispersion (ORD), which is the measurement of how the optical rotation of chiral molecules changes with wavelength. It covers the fundamental principles of ORD, instrumentation, the Cotton effect phenomenon near absorption bands, and applications of ORD such as determining absolute configuration and conformations. Specific topics include circular dichroism, polarimetry, and ORD applications in quantitative analysis, stereochemistry, and equilibrium studies.

Uploaded by

Til Sen
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Introduction

Fundamental Principles of ORD

Circular Dichroism

Instrumentation

Cotton Effect

Applications of ORD and CD

Specific applications of ORD

References
Introduction

Optical rotatory dispersion is the variation in the optical


rotation of a substance with a change in the wavelength of
light.

Optical rotatory dispersion can be used to find the absolute


configuration of metal complexes.

For example, when plane-polarized white light from an


overhead projector is passed through a cylinder of sucrose
solution, a spiral rainbow is observed perpendicular to the
cylinder.
Introduction
Introduction

Chiral molecules exhibit circular birefringence, which means


that a solution of a chiral Substance presents an anisotropic
medium through which left circularly polarised (L-CPL)
&Right circularly polarised (RCPL) propagate at different
speeds.

This leads to optical rotation which is measured by using a


polarimeter.

Measuring optical rotation as a function of wavelength is


termed Optical rotatory dispersion (ORD) spectroscopy
FUNDAMENTAL PRINCIPLES OF ORD:
FUNDAMENTAL PRINCIPLES OF ORD:

Light from ordinary lamp consists of waves vibrating in many different


planes. When it is passed through polaroid lense it is found to vibrate in
one plane is said to be plane polarised light or polarised light.
FUNDAMENTAL PRINCIPLES OF ORD:

The compounds which are having the ability to rotate the


plane of polarised light are called Optically active
compounds.
This property of compound is called optical activity. It is
measured by Polarimeter.
FUNDAMENTAL PRINCIPLES OF ORD:
Compound which rotates plane of polarised light to right(clock
wise) is called DEXTROROTATORY & is denoted by (+)
sign.If the compound rotates plane of polarised light towards
left side (anti clock wise) is called LEVOROTATORY & is
denoted by (-) sign.
FUNDAMENTAL PRINCIPLES OF ORD:
Enantiomers are optically active compounds. Optically active
molecules have different refractive indices, and different extinction
coefficients for L and R circularly polarised light

For a compound to be optically active it must be devoid of the


following properties

2. Center of symmetry (i)


3. Alternating rotation reflection axis of symmetry or an improper
axis (s)
FUNDAMENTAL PRINCIPLES OF ORD:
Rotation of plane polarized light(FRESNELS EXPLANATION):
According to Fresnel, a plane polarized light may be
considered as the combination of two circularly polarized light
of which one is right circularly polarized light (RCPL) & other
is left circularly polarized light (LCPL) which are in equal &
opposite in nature.

RCPL+LCPL
=
Plane Polarized light
(PPL)
FUNDAMENTAL PRINCIPLES OF ORD:
FUNDAMENTAL PRINCIPLES OF ORD:
If Refractive index is same for two circularly polarized light
then it vibrate in opposite direction with the same angular
velocity.

In the below image we can observe the change of E


FUNDAMENTAL PRINCIPLES OF ORD:

plane-polarized light is passed through a sample with a path


length of 1 decimeter and a sample concentration of 1 gram
per 1 milliliter.

Where,
Angle of Rotation in degrees
l path length is in decimeters
d
T Temperature.
FUNDAMENTAL PRINCIPLES OF ORD:
FUNDAMENTAL PRINCIPLES OF ORD:

Circular Birefringence is the difference in refraction (and the


associated speed of light) of left and right circularly polarized
light.
The polarization plane of linearly polarized light traversing a
Circularly Birefringent medium is rotated.
FUNDAMENTAL PRINCIPLES OF ORD:

The rotation is the result of an increment in the relative phase of the


left and right circularly polarized components in which the plane
polarized light can be decomposed.
The increment (which can be either positive or negative) is caused
by differences in the interactions of left and right circularly
polarized light with a medium consisting of chiral molecules.
FUNDAMENTAL PRINCIPLES OF ORD:

Optical rotation caused by compound changed with


wavelength of light was first noted by Biot in 1817.

The rotation angle is given by

Where,
l is the path length traversed by the light
nL and nR are the Refractive Indices for left and right
circularly polarized light of wavelength
Circular Dichroism
Some materials posses special properties of absorption of the
left circular polarised light to different extent than the right
circularly polarised light.
When the component emerges out there is an imbalance in
their strength & the resultant two will not be linearly polarized
but elliptically polarized & this phenomenon is called as
CIRCULAR DICHROISM.
Circular Dichroism
INSTRUMENTATION
A Polarimeter consists of following parts
Light source

Polarizer

Faradays
modulator
Sample cell

Analyzer

Detector Schematic representation of Polarimeter


COTTON EFFECT:
The Cotton effect is the characteristic change in optical
rotatory dispersion in the vicinity of an absorption band of a
substance.

The combination of both (circular birefringence and circular


dichroism) effects in the region in which optically active
absorption bands are observed gives rise to a phenomenon
called cotton effect.

The difference nL-nR eL-eR


length and can be positive or negative.
COTTON EFFECT:

Djerassi & Klyne suggested that rotatory dispersion curves


(i.e. plot of optical rotation against wavelength.) should be
classified in to two main types.

1. Plain curves

2. Cotton effect curves.


COTTON EFFECT:

These are normal or plain curves.


These curves occur at absorption maximum.

absorption in wavelength where optically active compounds


are examined. Ex: Hydrocarbons, Alcohols etc
COTTON EFFECT:

These curves will show the high peaks & troughs which
depends on the absorbing groups.
These curves will obtain for the compounds which are having
asymmetric carbon & chromophore which absorbs near UV
region.
These are again divided into 2 types, they are

1. Single cotton effect curves

2. Multiple cotton effect curves.


COTTON EFFECT:

These single cotton curves will show both maximum &


minimum curves at maximum absorption.

From the longer wavelength towards the cotton effect region if


crest passes first through it then the trough it is called as +ve
cotton effect.

In the same manner if it happens in opposite way it is called as


ve cotton effect.
COTTON EFFECT:
COTTON EFFECT:

These are a little different from the single cotton effect curves.
Here more than two crests & troughs are obtained. Ex:
camphor etc.
DIFFERENECES BETWEEN ORD AND CD:
OPTICAL ROTATORY DISPERSION CIRCUALR DICHROISM
(ORD) (CD)

ORD is If the refractive indices of the Circular dichroism is the differential


sample for the left and right handed absorption of left and right handed
polarized light are different, when the circularly polarized light
components are recombined , the plane
polarized radiation will be rotated through

ORD spectra are dispersive CD spectra are absorptive


In ORD the circular polarized light is used In CD the circular polarized lgiht is used
is not converted to elliptical light and is converted to elliptical light

ORD graphs are obtained by plotting CD graphs are obtained by ploting molar
specific rotation vs wavelength ellipticity vs wavelength.
APPLICATIONS OF ORD AND CD:
Determination of optically active substance such as amino
acids, polypeptides, proteins, steroids, antibiotics, terpenes.

Stereochemistry of Aliphatic amino acids: Aliphatic amino


-amino acids of levo
configuration show positive effect around 215nm while dextro
enantiomers show negative effect.

Stereochemistry of Steroids: In one form the specific rotation


increases with decreasing wave length(positive curve) and in
the other form the specific rotation decreases with increasing
wave length( negative curve) .
SPECIFIC APPLICATIONS OF ORD:

Quantitative Analysis: specific rotation is a good measure of


concentration.

Determination of Absolute configuration.

Conformational studies example :(+) 3 methyl cyclohexanone.

Equilibrium studies: If an optically active chromophore takes


part in a reaction the extent of reaction can be observed by
means of cotton effect.
Organic spectroscopy by William kemp 3 rd edition pages no
279-280.

Instrumental method of Chemical Analysis by Chatwal G.R.


and Anand S.K.

Instrumental method of Analysis by Willard H.H.,Merritt L.L.,


Dean J.A., Settle F.A., 6 th edition.

Instrumental methods of Chemical Analysis by B.K. Sharma.

Internet

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