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Experiment 1 SBU 1043

This document contains details of a practical report on an experiment to produce methane from food waste and garbage. The experiment used two bottles, one containing a mixture of food waste and the other serving as a control. By the seventh day of the experiment, the balloon attached to the bottle with food waste had expanded due to the production of methane by methanogens, while the control balloon showed no change. The report discusses methanogenesis, the microbes involved, and concludes that properly managing organic waste can help produce renewable energy while reducing greenhouse gas emissions.

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VENOSYAH A/P KALITHEVAN
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109 views4 pages

Experiment 1 SBU 1043

This document contains details of a practical report on an experiment to produce methane from food waste and garbage. The experiment used two bottles, one containing a mixture of food waste and the other serving as a control. By the seventh day of the experiment, the balloon attached to the bottle with food waste had expanded due to the production of methane by methanogens, while the control balloon showed no change. The report discusses methanogenesis, the microbes involved, and concludes that properly managing organic waste can help produce renewable energy while reducing greenhouse gas emissions.

Uploaded by

VENOSYAH A/P KALITHEVAN
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Course name: Emerging Issues in Biology

Course code: SBU 1043


Practical report on: Biological Instruments
(Experiment 1- Methane production from food wate and
garbage)
Date and day: 21/3/2021 – Saturday
Time: 9.30 a.m.

Name Matric number


HAFIZ IZZUDIN BIN RAMLI E20191023499
MOHAMAD HUSNUL HAKIM BIN MOHD HARIS
E20191023446
FADZILAH
VENOSYAH A/P KALITHEVAN E20191023515
E20191023532
DHIVIYAABARAATHI A/P SAMUGA VALOO
MUHAMAD ASYRAAF BIN ABDUL MANAN E20181020015
Experiment 1: Methane production from food waste and garbage
Title: Methane production from food waste
Objective:

 To learn about methanogens


 To understand the chemistry behind the production of methane from food waste
Material:

 Two 100 ml mineral water bottles


 Blender
 Tap water
 Food waste (cooked rice, vegetable soup, fried fish and rotten banana)
 Two balloons
 Rubber bands
Jotter:
Result:

Figure 1: Observation on the seventh day

Discussion:
In this experiment, we focus on the production of methane gas, to prove that food waste has
its potential to produce the optimum methane gas. From the material tested in this
experiment, we can conclude that the experiment showed a positive impact on the methane
yield. Comparing the balloon of the bottle, Bottle A and B, we can say that balloon on the
bottle A has expanded because methane gas is produced and trapped by the balloon whereas
there is no change in the balloon size of bottle B. Methane gas is produced by methanogens.
Methanogens are microorganism. They are prokaryotic and belong to the domain of archaea.
Methanogens are microorganism. They grow in organic food waste at a temperature of 80°C -
100°C. Since the bottle of waste material is exposed to direct sunlight and has a high
temperature, the experiment gave a better result. Methanogen carries out the methanogenesis
process (anaerobic respiration) that generates methane as the final product of metabolism. In
aerobic respiration, organic food waste such as glucose is oxidized to carbon dioxide, CO 2
and oxygen, O2 is reduced to molecule water, H2O. In contrast, during methanogenesis,
hydrogen gas, H2 is oxidized to ion hydrogen, H+ and carbon dioxide, CO2 is reduced to
methane, CH4 which is produced in this experiment.
Conclusion:
Methane is produced by methanogens. Methanogens are prokaryotes. They carry out the
methanogenesis process. In aerobic respiration, organic food waste such as glucose is
oxidized to carbon dioxide, CO2 and then during methanogenesis (anaerobic respiration),
carbon dioxide is reduced to methane, CH4. The issue of anaerobic digestion of organic waste
to produce biogas that can replace fossil fuels as our primary energy. The main constituents
of biogas are carbon dioxide and methane. Methane is the carbon-neutral energy source that
burns cleanly to produce carbon dioxide and water. Anaerobic digestion of organic wastes is
a sustainable practice to produce a clean-burning, carbon-neutral source, but the process must
first be refined. When we waste food, we also waste all the energy and water it takes to grow,
harvest, transport, and package it. And if food goes to the landfill and rots, it produces
methane—a greenhouse gas even more potent than carbon dioxide. therefore, organic waste
should be managed in a proper way to conserve the environment.

Reference:

1. Define me. (n.d.). Retrieved April 1, 2021, from https://www.cell.com/current-


biology/comments/S0960-9822(18)30623-7

2. Environmental and Energy Study Institute (EESI). (n.d.). Fact sheet: Biogas:
Converting waste to energy. Retrieved April 1, 2021, from
https://www.eesi.org/papers/view/fact-sheet-biogasconverting-waste-to-energy

3. Guest. (n.d.). Energy and Sustainability 2 (WIT transactions on ecology and the
environment) - PDF free download. Retrieved April 16, 2021, from
https://epdf.pub/energy-and-sustainability-2-wit-transactions-on-ecology-and-the-
environment.html

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