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Laboratory Report - DNA Extraction

This document describes an experiment to extract DNA from wheat germ. The key steps are: 1) Adding water and wheat germ to a graduated cylinder and stirring for 3 minutes. 2) Adding detergent and mixing gently for 5 minutes to break cell walls and membranes. 3) Slowly pouring ethanol into a test tube containing the wheat germ mixture. 4) Adding drops of phenol red, which detects DNA acids and changes color, confirming DNA extraction.

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Cjm Moya
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© Attribution Non-Commercial (BY-NC)
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3K views

Laboratory Report - DNA Extraction

This document describes an experiment to extract DNA from wheat germ. The key steps are: 1) Adding water and wheat germ to a graduated cylinder and stirring for 3 minutes. 2) Adding detergent and mixing gently for 5 minutes to break cell walls and membranes. 3) Slowly pouring ethanol into a test tube containing the wheat germ mixture. 4) Adding drops of phenol red, which detects DNA acids and changes color, confirming DNA extraction.

Uploaded by

Cjm Moya
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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a
 
p
p One Gram of wheat germ on graduated cylinder.p

p ÷dd 20ml (50-60) water. Stir for 3 minutes. p

ppppppppppppppppp p

p
3.p ÷dd 1ml of detergent and mix gently for 5 minutes.

pppppppppppppppp p

p
.p ÿilt test tube and slowly pour 10ml of ethanol.
a
 
 

5.p cet test tube sit for 3- minutes.

6.p ÷dd drops of phenol red.


0     
p

Goal: Investigate how DN÷ can be extracted from wheat germ

Materials:
-p Detergent
-p Ethanol
-p 50° - 60° tap water
-p Graduated cylinder
-p Phenol red indicator
[ 

1.p rhere is DN÷ found in the cell?

DN÷ is found in the nucleus of the cell.

2.p rhat was the function of the detergent in the experiment?

ÿhe function is to break the cell wall and cell membrane.

3.p rrite the 3 roles of DN÷.


-p ÿransmit information
-p Copy information
-p Store Information
.p Do all living organisms have DN÷?

Yes, because every living organism has a nucleus where DN÷ is stored.

5.p rhat Happens when we add phenol red to the DN÷?

It will show the DN÷ since phenol red is an acid detector and turn the color of
the water yellow.
d
 
1.p Did we achieve our goal? How.

Yes, because our goal was to investigate how DN÷ can be extracted from
wheat germ. re did it by making an experiment where we added water to the
wheat germ, added detergent to break the wheat germ cell wall and cell
membrane, and ethanol. re put all this together because to extract DN÷ you
need chemical means because you can¶t do it with a hammer since is really
small. ÷ll these chemicals (water, detergent, and ethanol) we put in where to
extract the DN÷ from wheat germ which is our goal. I learn that to extract
DN÷ you need chemicals not physical means and that the DN÷ play three roles
which are to transmit, copy, and store information.

2.p How did we know we extracted DN÷?

re know how we extracted DN÷ by using a chemical called Phenol Red which
detects any acid and since DN÷ has acids we Put some drops to see if we had
extracted DN÷ because it detects an acid changes from red color to yellow
color, this let us know if we had extracted DN÷.

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