04.1 Commercially Available Services Mycotoxins_2021 CEN-EN-ISO methods (food&feed).

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List of CEN, EN & ISO methods & general requirements for

mycotoxin analysis in food and feed
Standards must be purchased from CEN

Matrix Mycotoxin(-s) Method / link Description

General
Food analysis - Biotoxins - Criteria of
Food requirements for CR 13505:1999
analytical methods of mycotoxins
all mycotoxins
General Foodstuffs - Sample comminution for
Food requirements for CEN/TR 15298:2006 mycotoxins analysis - Comparison between
all mycotoxins dry milling and slurry mixing
General Food analysis - Performance criteria for
Food requirements for CEN/TR 16059:2010 single laboratory validated methods of
all mycotoxins analysis for the determination of mycotoxins
Animal feeding stuffs - Methods of sampling
General and analysis - Performance criteria for single
Feed requirements for CEN/TS 17455:2020 laboratory validated and ring-trial validated
all mycotoxins methods of analysis for the determination of
mycotoxins
Foodstuffs - Determination of aflatoxin B1,
Cereals, Aflatoxin B1 and the sum of aflatoxins B1, B2, G1 and G2
shell fruits & Aflatoxin B2 in cereals, shell-fruits and derived products -
EN 12955:1999
derived Aflatoxin G1 High performance liquid chromatographic
products Aflatoxin G2 method with post column derivatisation and
immunoaffinity column clean up
Foodstuffs - Determination of aflatoxin B1,
Cereals, Aflatoxin B1
and the total content of aflatoxins B1, B2, G1
nuts & Aflatoxin B2
ISO 16050:2003 and G2 in cereals, nuts and derived products.
derived Aflatoxin G1
High-performance liquid chromatographic
products Aflatoxin G2
method
Foodstuffs - Determination of aflatoxin B1
Hazelnuts,
and the sum of aflatoxin B1, B2, G1 and G2
peanuts, Aflatoxin B1
in hazelnuts, peanuts, pistachios, figs, and
pistachios, Aflatoxin B2
EN 14123:2007 paprika powder. High performance liquid
figs & Aflatoxin G1
chromatographic method with post-column
paprika Aflatoxin G2
derivatisation and immunoaffinity column
powder
clean-up
Animal feeding stuffs -- Determination of
Animal
aflatoxin B1 content of mixed feeding stuffs
feeding Aflatoxin B1 ISO 14718:1998
-- Method using high-performance liquid
stuffs
chromatography
Animal feeding stuffs - Determination of
Feed Aflatoxin B1 EN ISO 17375:2006
aflatoxin B1 (ISO 17375:2006)
Foodstuffs - Determination of aflatoxin B1 in
cereal based foods for infants and young
Cereal
Aflatoxin B1 EN 15851:2010 children - HPLC method with immunoaffinity
based food
column clean-up and fluorescence detection
C
Foodstuffs - Determination of aflatoxin B1,
Cereals, Aflatoxin B1
and the total content of aflatoxins B1, B2, G1
nuts & Aflatoxin B2
EN ISO 16050:2011 and G2 in cereals, nuts and derived products
derived Aflatoxin G1
- High-performance liquid chromatographic
products Aflatoxin G2
method (ISO 16050:2003)
Aflatoxin B1 Foodstuffs - Determination of aflatoxins in
Food:
Aflatoxin B2 spices other than paprika by IAC clean-up
spices other EN 17424:2020
Aflatoxin G1 and HPLC-FLD with post-column
than paprika
Aflatoxin G2 derivatization
Milk & milk Milk and milk products. Guidelines for a
Aflatoxin M1 ISO 14675:2003
products standardized description of competitive
 04.1 Commercially Available Services Mycotoxins_2021 CEN-EN-ISO methods (food&feed).docx

enzyme immunoassays. Determination of

aflatoxin M1 content
Milk and milk powder. Determination of
Milk & milk aflatoxin M1 content. Clean-up by
Aflatoxin M1 ISO 14674:2005
powder immunoaffinity chromatography and
determination by thin-layer chromatography
Milk and milk powder. Determination of
ISO 14501:2007
aflatoxin M1 content. Clean-up by
Milk & milk Will be replaced by
Aflatoxin M1 immunoaffinity chromatography and
powder prEN ISO 14501
determination by high-performance liquid
(under approval)
chromatography (ISO/DIS 14501:2019)
Traditional Chinese medicine --
Natural
Aflatoxins ISO/CD 22283:2020 Determination of Aflatoxins in natural
products
products by LC-FLD
Foodstuffs - Determination of citrinin in food
Foodstuffs Citrinin EN 17203:2018 by liquid chromatography tandem mass
spectrometry (LC-MS/MS)
Foodstuffs - Determination of ochratoxin A in
cereals and cereal products - Part 1: High
EN ISO 15141-
performance liquid chromatographic method
Cereals & 1:1998
with silica gel clean up
cereal based Ochratoxin A
Foodstuffs - Determination of ochratoxin A in
products EN ISO 15141-
cereals and cereal products - Part 2: High
2:1998
performance liquid chromatographic method
with bicarbonate clean up
Barley & Foodstuffs - Determination of ochratoxin A in
roasted Ochratoxin A EN 14132:2009 barley and roasted coffee - HPLC method
coffee with immunoaffinity column clean-up
Foodstuffs - Determination of ochratoxin A in
Wine & beer Ochratoxin A EN 14133:2009 wine and beer - HPLC method with
immunoaffinity column clean-up
Currants,
Foodstuffs - Determination of ochratoxin A in
raisins,
currants, raisins, sultanas, mixed dried fruit
sultanas,
Ochratoxin A EN 15829:2010 and dried figs - HPLC method with
mixed dried
immunoaffinity column cleanup and
fruit and
fluorescence detection
dried figs
Foodstuffs - Determination of ochratoxin A in
Cereal cereal based foods for infants and young
Ochratoxin A EN 15835:2010
based food children - HPLC method with immunoaffinity
column cleanup and fluorescence detection
Animal feeding stuffs - Determination of
Ochratoxin A in animal feed by
Animal feed Ochratoxin A EN 16007:2011 immunoaffinity column clean-up and High-
Performance Liquid Chromatography with
fluorescence detection
Cereals and cereal products - Determination
Cereals and of ochratoxin A - High performance liquid
cereal Ochratoxin A ISO 15141:2018 chromatographic method with
products immunoaffinity column clean-up and
fluorescence detection
Food:
paprika,
chilli, black Foodstuffs - Determination of ochratoxin A in
& white paprika, chilli, black & white pepper, nutmeg,
pepper, spice mix, liquorice, cocoa and cocoa
nutmeg, Ochratoxin A EN 17250:2020 products by immunoaffinity column clean-up
spice mix, and high-performance liquid
liquorice, chromatography with fluorescence detection
cocoa and (HPLC-FLD)
cocoa
products
Food: Foodstuffs - Determination of ochratoxin A in
pork meat EN 17251:2020 pork meat and derived products by high
Ochratoxin A
and derived performance liquid chromatography with
products fluorescence detection (HPLC-FLD)
 04.1 Commercially Available Services Mycotoxins_2021 CEN-EN-ISO methods (food&feed).docx

Foodstuffs - Determination of deoxynivalenol

Cereals & in cereals, cereal products and cereal based
cereal based Deoxynivalenol EN 15891:2010 foods for infants and young children - HPLC
food method with immunoaffinity column clean-
up and UV detection
Animal feeding stuffs - Determination of
Animal
zearalenone by immunoaffinity column
feeding Zearalenone ISO 17372:2008
chromatography and high-performance
stuffs
liquid chromatography
Animal feeding stuffs - Determination of
Animal zearalenone in animal feed - High
feeding Zearalenone EN 1592:2009 performance liquid chromatographic
stuffs method with fluorescence detection and
immunoaffinity column clean-up
Maize based
baby food, Foodstuffs - Determination of zearalenone in
barley flour, maize based baby food, barley flour, maize
maize flour, flour, polenta, wheat flour and cereal based
Zearalenone EN 15850:2010
polenta, foods for infants and young children - HPLC
wheat flour method with immunoaffinity column clean-
and cereal up and fluorescence detection
based food
Vegetable Foodstuffs - Determination of zearalenone in
Zearalenone EN 16924:2017
oils edible vegetable oils by LC-FLD or LC-MS/MS
Apple juice,
apple juice Apple juice, apple juice concentrates and
concentrates drinks containing apple juice. Determination
Patulin ISO 8128-1:1993
and drinks of Patulin content. Part 1: Method using high-
containing performance liquid chromatography
apple juice
Apple juice,
apple juice Apple juice, apple juice concentrates and
concentrates drinks containing apple juice. Determination
Patulin ISO 8128-2:1993
and drinks of Patulin content. Part 2: Method using thin-
containing layer chromatography
apple juice
Foodstuffs - Determination of Patulin in clear
Apple juice and cloudy apple juice and puree - HPLC
Patulin EN 14177:2003
& puree method with liquid/liquid partition clean-up

Foodstuffs - Determination of Patulin in fruit

Fruit juice & juice and fruit-based purée for infants and
fruit-based Patulin EN 15890:2010 young children - HPLC method with
puree liquid/liquid partition clean-up and solid
phase extraction and UV detection
Foodstuffs - Determination of fumonisins B1
Fumonisin B1
Maize EN 13585:2002-03 and B2 in maize - HPLC method with solid
Fumonisin B2
phase extraction clean-up
Document title Foodstuffs - Determination of
Maize based Fumonisin B1 fumonisin B1 and B2 in maize based foods -
EN 14352:2004
food Fumonisin B2 HPLC method with immunoaffinity column
clean up
Animal feeding stuffs - Determination of
the Sum of Fumonisin B1 & B2 in
Animal
Fumonisin B1 compound animal feed with
feeding EN 16006:2011
Fumonisin B2 immunoaffinity clean-up and RP-HPLC
stuffs
with fluorescence detection after pre- or
post-column derivatisation
Foodstuffs - Determination of fumonisin B1
Processed and fumonisin B2 in processed maize
maize Fumonisin B1 containing foods for infants and young
CEN/TS 16187:2011
containing Fumonisin B2 children - HPLC method with immunoaffinity
food column clean-up and fluorescence detection
after precolumn derivatization
 04.1 Commercially Available Services Mycotoxins_2021 CEN-EN-ISO methods (food&feed).docx

Food:
Processed Foodstuffs - Determination of fumonisin B1
maize and fumonisin B2 in processed maize
containing Fumonisin B1 containing foods for infants and young
EN 16187:2015
foods for Fumonisin B2 children - HPLC method with immunoaffinity
infants and column clean-up and fluorescence detection
young after pre-column derivatisation
children
T-2 toxin Animal feeding stuffs: Methods of sampling
Animal feed HT-2 toxins and analysis - Determination of T-2 and HT-
EN 16877:2016
Deoxynivalenol 2 toxins, Deoxynivalenol and Zearalenone, in
Zearalenone feed materials and compound feed by LC-MS
Food:
cereals and
Foodstuffs - Determination of T-2 toxin and
cereal
T-2 HT-2 toxin in cereals and cereal products for
products for EN 16923:2017
HT-2 infants and young children by LC-MS/MS
infants and
after SPE clean-up
young
children
Ochratoxin A
Aflatoxin B1 Foodstuffs - Multimethod for the screening of
Deoxynivalenol ochratoxin A, aflatoxin B1, deoxynivalenol,
Zearalenone zearalenone, T2-toxin and HT-2 toxin and
Food EN 17279:2019
T-2 toxin fumonisin B1 and B2 in foodstuffs, excluding
HT-2 toxin foods for infants and young children,
Fumonisin B1 by LC-MS/MS
Fumonisin B2
Ochratoxin A
Aflatoxin B1 Animal feeding stuffs: Methods of sampling
Deoxynivalenol and analysis - Determination of
Zearalenone Deoxynivalenol, Aflatoxin B1, Fumonisin
Animal feed EN 17194:2019
T-2 toxin B1&B2, T-2 & HT-2 toxins, Zearalenone and
HT-2 toxin Ochratoxin A in feed materials and
Fumonisin B1 compound feed by LC-MS/MS
Fumonisin B2
Aflatoxin B1
Deoxynivalenol Foodstuffs - Multimethod for the screening of
Fumonisin B1 aflatoxin B1, deoxynivalenol, fumonisin B1
Fumonisin B2 and B2, ochratoxin A, T-2 toxin, HT-2 toxin
Food EN 17279:2019
Ochratoxin A and zearalenone in foodstuffs, excluding
T-2 toxin foods for infants and young children, by LC-
HT-2 toxin MS/MS
Zearalenone
Zearalenone Foodstuffs - Determination of zearalenone
Deoxynivalenol and trichothecenes including deoxynivalenol
Food
3-acetyl-DON and its acetylated derivatives (3-acetyl-
Cereal &
15-acetyl-DON EN 17280:2019 deoxynivalenol and 15-acetyl-
cereal
Nivalenol deoxynivalenol), nivalenol T-2 toxin and HT-
products
T-2 toxin 2 toxin in cereals and cereal products by LC-
HT-2 toxin MS/MS
Aflatoxins
Deoxynivalenol
Foodstuffs - Multimethod for the
Fumonisins prEN 17641
determination of aflatoxins, deoxynivalenol,
Food Ochratoxin A (WI 00275369)
fumonisins, ochratoxin A, T-2 toxin, HT-2
T-2 toxin (Under Enquiry) toxin and zearalenone by LC-MS/MS
HT-2 toxin
Zearalenone
ergocornine
ergocorninine
ergocristine Animal feeding stuffs: Methods of sampling
Feed
ergocristinine and analysis - Determination of ergot
materials &
α-ergocryptine EN 17256:2019 alkaloids and tropane alkaloids in feed
compound
α-ergocryptinine materials and compound feeds by LC-
feed
ergometrine MS/MS
ergometrinine
ergosine
 04.1 Commercially Available Services Mycotoxins_2021 CEN-EN-ISO methods (food&feed).docx

ergosinine
ergotamine
ergotaminine
atropine
scopolamine
ergocornine
ergocorninine
ergocristine
ergocristinine
α-ergocryptine
Cereals & Foodstuffs - Determination of ergot
α-ergocryptinine EN 17425:2020
cereal alkaloids in cereals and cereal products
ergometrine (approved)
products by dSPE clean-up and HPLC-MS/MS
ergometrinine
ergosine
ergosinine
ergotamine
ergotaminine
Altenuene
Food: Alternariol
tomato, Alternariol prEN17521 Foodstuffs - Determination of Alternaria
wheat, monomethyl (WI=00275285) toxins in tomato, wheat and sunflower
sunflower ether (under approval) seeds by SPE clean-up and HPLC-MS/MS
seeds Tentoxin
Tenuazonic acid