Scribd
Upload
336 views1 page

RT-PCR Report

This document contains the results of a RT-PCR test performed on a nasopharyngeal and oropharyngeal sample collected from a 29-year old female patient. The test was negative for COVID-19 as both the E gene and RdRp/S gene markers were not detected. A single negative result does not rule out infection, and the report notes limitations including early or late sample collection as well as the possibility of false negatives.

Uploaded by

Anuj Sharma
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
336 views1 page

RT-PCR Report

This document contains the results of a RT-PCR test performed on a nasopharyngeal and oropharyngeal sample collected from a 29-year old female patient. The test was negative for COVID-19 as both the E gene and RdRp/S gene markers were not detected. A single negative result does not rule out infection, and the report notes limitations including early or late sample collection as well as the possibility of false negatives.

Uploaded by

Anuj Sharma
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 1

Date 24/06/2021 Patient ID 142077613

Name. Mrs. Shalini Gemini SP Coll.24/06/2021 14:42:44


Sex / Age Female/29 Yrs SP Rec. 24/06/2021 15:03:46
Ref by Other SELF Auth Dt 26/06/2021 16:25:27
SRF ID 0708200541078 Print Dt. 26/06/2021 16:30:15
Sample Nasopharyngeal & Oropharyngeal

Molecular Biology
RTPCR TEST FOR COVID 19 SAMPLE COLLECTED
AT CENTRE
E Gene / N Gene Negative
RdRp/S Gene Negative
Final Result Negative

INTERPRETATION
RESULT COMMENT
E gene Negative Absence of B Beta CoV RNA indicate no infection with lineage B-betacoronavirus
Both E gene AND Orf1A/RDRP gene Negative Absence of B-Beta CoV RNA and SARS-CoV RNA indicate no infection with lineage B-
betacoronavirus
E gene Positive AND Orf1A/RDRP gene Presence of B Beta-CoV RNA indicates infection with lineage betacoronavirus virus BUT
Negative absence of COVID-19
Both E gene AND Orf1A/RDRP gene Positive Presence of B Beta-CoV RNA and SARS-CoV RNA indicate infection with COVID-19
E gene Negative but Orf1A/RDRP gene Positive Inconclusive, As per guidelines a repeat sample is recommended.
A Single negative test result, particularly if this is from an upper respiratory tract specimen, does not exclude infection. False Negative results may be
seen in samples collected too early or late in the clinical course of the infection. Close monitoring of symptoms and retesting may be considered, as per
clinical evaluation. The repeat specimen may be considered after a gap of 2-4 days after the collection of the first specimen for additional testing, if
required.
TEST ATTRIBUTES AND LIMITATION
1. Samples must be received at the laboratory under appropriate conditions within 48 hours of collection to ensure preservation of viral RNA.
2. Negative result does not rule out the possibility of COVID-19 infection. Presence of inhibitors, mutations and insufficient organisms RNA can influence
the result. False Negative result may be seen in samples collected too early ore too late in the clinical course of the infection. Kindly refer to the
latest ICMR guidelines.
3. A single negative test result, particularly if this is from an upper respiratory tract specimen does not exclude infection. A positive alternate pathogen
does not necessarily rule out COVID-19 infection, as little is yet known about the role of co-infections.
4. Repeat sampling and testing of lower respiratory specimen is strongly recommended in severe or progressive disease. The repeat specimen may be
considered after a gap of 2-4 days after the collection of the first specimen for additional testing if required.
Test is done on: Real Time Polymerase Chain Reaction (RT-PCR).
ICMR Registration No GDICPLD.

*** End of Report ***

DR MANOJ KUMAR DR SHAHEEN BHAT DR. RADHIKA KHANNA


M.Sc, M.Phil,PhD (AIIMS) MBBS,MD MSc, Ph.D
Microbiologist Consultant Microbiologist Consultant Microbiology
www.busysoft.in

Checked By.: RADHIKA_KHANNA


Page No: 1 of 1

You might also like