Manual Do Microscópio
Manual Do Microscópio
.,
1
I
Olympus System M i m p e Series BH
transformer h i l t in the base, a unique c ~ d pr-s
q
applid to the prisms in tfie abservadan t u k ta ~~ light
ents such as microscope tranmi~iontnm than twice w much as in mnwntimal
observation tu& elk.
Every part of the Series BH mmpownts and a c m o r i s is
&signed with emphasis on durability; fix imtanwJs ~ o 8 t h
lunnink wear resistant ball guides are employed in the f w w
mudular, buil&rl~blwksystems provides ing and stage operatian mechanisms, and the light wm
m k r m p i c and pbtom icro- built in the km incorporates a thyristor circuit fw 5bMliza-
#a& ambhtatiom m a suitable for your requirements, to tion bf power wpply.
, <.-.yWr at a reatSstEc figure; and to further expand 3. Amesswies for Expanded Capabilities
e6,m.qsb@w of the micrmcuge by addig msonably I
Dqendng upon your requirements for not only general but
to specif~cpurposes. atso specific purposes, vwious accessories are available for
bri@tfield, darkfield, fluorescene, polarization, phase con-
. trast, d8fferential interference contrast fur transmitted and
~ ~ t light;e dsuper widefield, dual viewing; and p h o t ~
miwogaphy, automatic and manual.
4.. V-dIe Camera System
The System Camera Model PM- 'I0-A with automatic film
~'advana fadliiates your photomicrographic work, solving
, the dtficult color temperature adjustments often encounter-
Excellent Wfbrmance Tn
Ob~~atio uk
Rtvalving Nosepis&
sfiw
.
observation tube5 with ease. A binocular tube and a super
widefield observation tube are available optionally.
Base
W FOCUSiNG ADJUSTMENTS ~ ~ . ~ e h . n f i s u
The I* qPU#rtment
*The dual coarse and w@ehnhrn opewee a
~ P wm-
I O C W plnwn -
fine focusing knobs mow to thrs and goarser
a rutmefit motion, o h
are coaxial and aYour+tage -re#-
tdon a Y t t @ m TAb unC
designed to share fgn permla fins
a common guide-
fW 2
o c u I ow? #he e r r W
rnF offha r n 0
=us=# rnoktrrsr~ta n J i
way to give a g @ t e a l l ~+e h m
c-h.
smooth, even The e w n e ddwtment
~mschonitm wrorks dl-
motion through- m t l u on the foclrplng
wbiuh laQtas
art the whole range FCik r ttac+hg~
~krF mouutw block'
iq t b
of 40mm. b$ -I& the .red&
T h e coarse adjust- S,E*i;. zi:
ment knobs are
56mm in diameter for
easy operation.
The fine focusing adjustment i s
graduated in minimum increments o
Zp and incorporates special reduction
gears for smooth operation and life-
time reliability.
The automatic pre-fwusing lever is
provided to prevent possible contact
between specimen and objective as
well as to simplify coarse fmusing.
The lever is tocke
This prevents turther upward travel of the stage and auto-
matically provides a limiting stop if the stage is lowered and
then raised again, Ihe automatic prefocusing lever dms not
restrict fine focusing. lBASE
.A tens~onadjustment ring i s provided next to the right The dua-pfE10f 5quaM
hand coarse adjustment knob. With this device the tension of base incorporates a h ighllow magni-
the coarse adjustment is treely adjustable for either heavy or fic+tion selcctor system and a l o w vuluge iramformer. The
light movement depending on operator preference. "omoff" switch, voltmeter and sliding control lever for
continuously variable light inten~itiyare hit t in the bde Tur
effortless operation.
The highllow magnification selector lever enables Koehler
.CONDENSERS type illumination throughout the whale range of objective
A ring mount with clamping screw and centering device powcrs, from 4X t o 100X, without rnanipulatiur~uf the
accepts interchangeable condensers, standard or optional. auxiliary lens or interchange of condensers.
Rack and pinion height ddjustment, with condenser height .The in-base illuminator serves to direct light of the
displacement up to 32mm. appropriate quality and intensity, -emitted from a precenter-
*The standard condensers include the achromaticlaplanatic ed tungsten bulb, towards the microscopic object to ensure
% condenser N.A. 1.40, the Abbe condenser N.A. 1.25, and the even illumination o f the field, This in turn results in
super widefield condenser N.A. 0.95, depending on the photomicmgraphic negatives of optimum contrast, briltiance
I* . model of your choice, for optimum illumination of the
... and uniformity.
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specimen from low power 4X to oil immersion lOOX The built-in transformer, variable from 0 V to 10 V,
objectives. Furthermore, the achromaticlaplanatic condenser incorporates a thyristor circuit in its rheostat far smooth fine
&, is corrected for chromatic' aberration, spherical aberration adjustment of light intensity.
and curvature of field, and is provided with a graduated .The standard light source incoporates a 30 watt pre
aperture iris diaphragm, decenterable for oblique illurnin* centered tungsten filament bulb, provided with a socket for
tion. It is recommended for work with high quality apo- positive contact, el irninating the problems of defective
chromats and plan apwhrornats. contact and overheating. Interchangeable with optional light
Ei?her one of the condensers, ahave, is easily replaceable sources such as high intensity halogen bulbs, mercury burners
with other condensen such as darkfield, phase contrast, etc. and eltctronic flash bulbs. a. :F 1
r. d -
Wrn Chart of Interchangeable Components 1
BH-0145 BKB130 Bli-TR45 BH-TR30 BH-SWTR BWPT
Binocular t u b (45') Binocular tube (3@) Trinocular tube (45') Trinocular t u b (30') Super widefield Vertical photot~be
winocular tube (30")
1
BH-Do M A AKFA BWFA AWN..
Dual viewing
'
..........................................................
IIH.CD BH-AAC BKSWC BH-DCD BH-DCW
LOW power Abbe condenser Achromatic/aplanatic Super widefield Dry darkfield
condenser condenser condenser condenser conlmr
BKPC BH-NC
Abbe phase contrast Differential Polarizing condenser
condenser interference contrast
condenser
.. .I...i........... .....................
- r. I
-
kP7R
I r~noculart u h for
I
I
= PM-CP
3-114"~4ll4"
Polaroid@film holder
1
be' polarizing light (30")
PM-C4~5
BKPBI L lnttrrnediate adapter
for 4 * xS* holders
I
L b
PM-1OA M I
Photomicr?flaphic Photomicrographic
'., BKMPS system camera system camera
i r :Projection screen (automatic) (manual)
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....................................... b
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I) I I I
[ a -
T5 - j
4 I: AI+-PA BKWL BH-RFA
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Intermediate Polarizing filter Vert~calilluminator
Drawing attachment
A
i polarizing tube set for fluorexenre BH-mRF
F Iuorescenr~
BI-l-PRE
Revolving
................................. lamp house
nowpime BH-RE
for polarizing ReVblVing
Iight nosepiece
...........................
-................................k
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Microscope
stands
BHA-F BHBF BHGF
B A
I
dE1
I
I530
LS15
Tungsten bulb Tungsten bulb
BH-LH BHLSH 6V .- 8V, 30W 6V, I S W
30-watt tungsten lobwatt halogen
lamp house lamp hwse
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MICROSCOPE STANDS BHA-I
Extremely sturdy construction, functional design, ripple tone
finish, acid resistant. The differences between these three
stands are the interchangeability of nosepiece andfor light
source with the Models BHA and BHB, respectively.
Revolving nosepiece
Coarse and
fine adjuszment and fine adjustments 40rnm, on ball guides.
45mm diameter filttrs and a polarizer can be mounted on the light exlt on the baa.
Filter mount An analyzer can be inserted below the observation tube.
I
In-base Illuminator without
In-base IIIurninator, with high/low rnagniflcatlon selector, magnlflcation wlecror. L
lllumtnatlngsystem - -
Maximum
field number** 29 21
Dlopter Adjustable
diopter setting
Both observation eyepiece tubes equipped with variable diopter adjustment, -
adjustment compensating for tube length variations.
on SW cyepieccs.
Convergent angle O" (parallel optical axes in binocular tubes) -
InterpupilJary 56 - 74mm,
distance 53 - 72mm, graduated.
adjustment graduated.
B H E t are interchangeable.
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&phMlc*l lens
CoRecttsr
F W %erJaStobEt PwforW
Vartous filters availabld I Frosted f l i e r buift-in
-
efficiency and speed of microscopic
work but will not introduce undesir-
able eyestrain.
STANDARD EQUIPMENT
Super widefield trinmular tube, inclined 30a,
A with vertical phototube BH-SWTR ............................ 'I
Super widefield condenser, N.A.0.95, with
I graduated aperture iris diaphragm BH-SWC ................. 1
Super widefield eyepieces BiSW1OX, paired ..................... 1
Photo eyepiece FK3.3X .......................................... 1
'Objectives: Plan 4X, Plan IOX, Plan XIX (spring),
Plan 40X (spring), SW Plan 100X (oil,
spring) (set of tive) ................................... I
Optional Accessory
Super widefield eyepieces SW7X, paired
*If your BH Microscope is equipped with Plan objectives,
objectives mentioned abwe except SW Plan 100X may be
omitted.
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Fluorescence Vertical Illurninator Attachment (after Ploem)/MODEl BH-RFL
The Model BH-RFL incorporates a
hi$ pressure mercury burner, a wide
range of exciter filters and barrier
filters in addition to all or either of 4 '
dichroic; mirrors for ultraviolet, v i e
let, blue and green excitation. The
dichroic mirrors, originated by Dr. j.
S. Plaem, are hilt in mounts for
selection of the desired wavelengths for
excitation of the specimen. Radiation
energy emitted from a powerful 100W
high-pressure mercury burner is trans-
mitted through wllectw and exciter Cat Brain, Acridlne Orange
filter to the dichroic mirror to excite
the fluorochrome in the specimen in
the desired wavelength. After irradia-
tion with the excitation wavelengths,
the specimen emits a longer visible
wavelength, due to Stoke's law. A
barrier filter between objective and eyepiece passes tbe
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- ., ..., Interference Contrast Attachment for ansmitted Light/MODEL BHNIc
The Model BH-NIC is designed for the tours more difficult The Nomarski
qualitative study of transmitted li&t method does not cause halos, so that
phase objjcts with the System Mime a clear definition of image details is
=opes Series BH. This differential obtained.
interference contrast device incorpo- .The Nomarski method permits utili-
rater a pair of birefringnt crystal zation of the full numerical aperture
prisms as beam-splitters, with a shear of the objective, which results in ex-
between the t w o interfering beams ceptioml image brightness and re-
smaller than the resalution limit of the solution almost double that of phase
objective ussd in the microscope, w contrast.
that no double image is observed. q- .The depth of fwus is smaller in dif-
The interference contrast method ferential interference contrast than
r ~ n d e ra~ sharply defined, relief-like in phase contrast. This prevents dis-
image with excellent contrast in a wide turbing out-of-focus effects, and
ran@ d interference colors. This permits layer by layer scanning of
method pwmits obmvation of un- specimens, commonly called "&ti-
stained transparent objects like phase cal sectioning".
contrast, which makes i t useful for o b Interference colors, or grey shadings,
sewation in histology, cytology, biw can be seen in proportion with the
logy, anatomy, etc. Furthermore, when used with the Series gradient of path differences of the specimen. P h m con-
BH, this attachment permits detection of minute differences trast helps to determine a high or low refractive index of a
of thickness and changes of internal refractive indexe-s of specimen detail by the appearance of halos, wording to
unstained, tramparent specimens By operating the bi- the positive or negative contrast of the objective in use.
refrinant crystal p r i m built in the intermediate t u b , a most
suitable contrast color can be chosen ,in accordance with AE t h e shearing direction i s restricted in accordance with
purpose and specimen. The background w l o r can be w n - the sliding directinn nf the Wnllaston prism, it is preferable
tinuwsly changd in the order from dark to yellow, to to use a rotatable stage to wient the specimen in the
purplish red, to blue or in the reversed order. direction b e s t suited fob optimum msolution.
Features For the observation of anisotropic objects, it is re-
+Generally the larger the phase difference, the more con- commended tn apply phase contrast rather than inter-
spicuous the halos, which is a drawback of the ordinary ference contrast for better results.
phase contrast method, making distinct observation of m-
STANDARD EQUIPMENT
Differential interference contrast intermediate tube
AH-NA. .........
%- Differential interference contrast condenser BH-NC
1
.........1
I Phase contrast objectives: PC PL Plan 10X .....................
PC PL Plan 40X ..................... 1
1
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and 100X phase objectives, which are available in *Negative medium contrast
4 contrasts, PLL, YL, NM, NH, and an empty PC NM Ad1 1 OX, PC NM A G 2~0 X ,
PC NM Ach S-40X (spring), PC NM Ach
aperture for brightfield observation. 5-1 00X (spring, oil) ...............,.,.
*The Model A-PC-PA consists of all the 4
1 ea.
'Ihese letters designate the following contrasts as listed above, while one con-
contrasts: N = Negative; P = Positive; M = trast combination can also be available as
Medium; L = Low; LL = Low-Low; H = High. fulluws:
1
B-PC-P&PL positive low contrast)
This exceptionally wide selection o f combinations is B-PC-PB-PLt positive low low contrast)
I certaln to s u ~ the
t most demanding requiternenrs. &PC-PB-NH r~eg.ilive I~lgllL L H I ~ ~ ~ L )
B-PC-PB-NM negative medlum contrast)
II
hkroscope stand ,
1, Transformer
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Model BH-P-1 includes a binocular tube for mounted in a rotating turret, with a depolarizer
polarizing observation, while the Model which makes the binocular vision of the spe-
8H-P-2 indudes a trinocular tube for both cimen evenly black between crossed analyzer
polarizing observation and photomicr* and polarizer.
graphy, in addition to the standard equip-
ment commonly shared between them.
Each of these attachments facilitates mi-
b The multi-layer coating applied to the
optical elements in the observation
tube's BH-PB1 and BH-PTR assures ex-
croscopic work in polarized light not ceptionally bright binwular vision.
m l y in the fields of mineralogy and
petrography, but also in chemistry,
pharmacology, biology, medical science
and ceramics, as well as in the chemical
and textile industnies.
657 To those who do not yet own the BHA
BHA-751-P are
micrompe, Models *HA-651-P or
therecommended.
b *
6'
I Projection ScreeniMC!@@
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1
Photomicrographic System Camera 1 MODEL PM-10
The PM-10 System Camera combines the features and
advantages of many separate conventional attachment
cameras, ranging from 35 mm camera back to 4" x 5" film
holders. The Model PM-10 consists of two basic units-a fully
automatic version Model PM-1 @A and a popular manual
version Model PM-10.M.
The modular design concept of the system camera permits
easy interchangeability of many accessories. When these
modular accessories are attached to the automatic or manual
photomicrographic bodies in accordance with your require-
ments, sharply focused pictures on standard 35 mrn film,
3%" x 4%" Polaroid film or 4" x 5" size film can easily be
obtained. The data imprinting 35 mrn camera back can, be
easily attached on the exposure body. Once in place, the
back enables the user to imprint informarion such as
numhrs, sy mbots, etc., on the film.
The Model PM-IOM and the Attachment Camera Model
PM-6 incorporate a light measuring port to accept the
exposure and color temperature probes of the Exposure
Meter Model EMM-7
0 & * 5* lnPs;msdla& ~wqpw
l( 1 4*~rr 5' ~ckoid@filmb k , G M m i c fnm holder,
der, G r a p h k h t film holdor) ( P W )
Back [PMEP)
@ 35mm Crmsn Back vlilk Manull film M c o (G35J
@ 35$lm k c k ~ 4 t hAutomatlf F i h A&- (C-3M)
0 ~withRL*y~fworFwmt~oElu(PM-DL)
@ Ahptw for 3 5 m 1- Bpck wIUI Manual Fllm Muarm (PM-D35)
@ fff 35mm b m f a Back vrih Automtk FIlm Ad*- (PM-DSSAJ
@ Cbntrd Unit for Fully A u m a t l c 35mm G m r a Back {PMEBA)
@ Expmum M b t a (EMM-7)
t
W.. T
speed 0.75,0.85, 1,1.2, 1.5.
Automatic comptnsatfon for
reciprocity failure.
Automatic exposure range 32 min. B and 1 rec. to 11250 sec. with integrated mechanical shutter in vibration
1 to 11100 stc. Push button for proof rubber mount
tima automatic Buttar retern, to elirni-
k natc shutter vibration.
L a m format Poimid 3%"x 4%"' 4" x 5" sheet film holdon, film pack holder. Not available.
attachments
Optical Characteristics
I n keeping with the varied requirements of the users, EYEPIECES
Olyrnpus makes available objectives of high resolution from TYae 1 Initial Magnification I ~ i e l dNumb
1.3X to 1OOX magnifications. They include Achromats, Plan
Achromats, Fluorites, Apochromats, Plan Apochromats and Compensating K20X 7-5
Super Widefield PIan Achrornats. Especially, the PIan Apo- BiK2OX 7-5
chromats are capable of producing a flat image to the edge of WF 10X 18
the field, with excellent resolution, free of field curvature. WF 1OXMicro 18
Chromatic aberration is corrected for three colors, spherical
aberration for two colors. The best objectives suitable for the
Widefield
I (with micrometer 10]100rnrn)
WF 15X
BiWF 1 OX 18
m&t sophisticated demands. BiWF 15X 12
All eyepieces are computed to match the objectives for full
chromatic and distortion correction. They are Compensating, High Eyepoint H.E.P. WF 1OX 18
Flat Field High-Eyepoint, Super Widefield, Photo Eyepieces,
Widefield H.E.P. BiWF 10X . 18
etc. BiSW 7X 29
Super Widefield BiSW OX 26.5
Photo FK 25X, FK 3.3X
FK 5X. FK 6.7X
Initial Magnification
-
4) 0.10 I 19.87 mm
1ox
20X
40X
60X
1OOX (oil)
S4OX (spring)
S-60X (spring)
5-100X (spring, oil)
1-100X (iris diaphragm)
No cwer Ach 40X (for use without cover glass)
J
*- FL S-40X (spring)
'-' 3 FL S-60X (spring, dry, correcting collar)
F I u a e s (Semi apocbroma**) FL S-1 ODX (wring, oil]
NOcover FL 40X (for usa without covet glass)
1ox
Achromats
mx
S-40X Irprina)
Phase Contrast S-100X (spring, oil1
Fluorites FL 5-40X (spring)
(Semi apochromao) FL S-100X (spring, oil)
Apo #X (spring, dty, catrestlngcdlar)
Apo 40X (spring, ail, iris diaphragm)
Plan 1.3X
Plan 2X
Plan 4X
Plan 1OX
Plan A c R m a t s Plan 20X (spring)
Super Widefield Plan 4UX (spring)
sw W ~ I II oox isprina oil) 1 ,2S 0,08
Plan 100X (wring, oil ) 1.25 0.14
Plan ADO4X 0.16 4.35
Plan A ~ 1 OOX (s~rinel 0.32 0.16
Plan Apochrmts Plan Apo 2QX (spone) 0.65 0.14 .
1
Pian Apo 4&X [spring, correctingcollar) 0.95 0.10
PIan Apo 10 0 X (spring, oil) 1.30 0.09
*: "'
Combina-tionsof Various Components 4
I,.
BHA-F
BHGF
I
--
A.
Achromat 1OX
Ran~chrbmat 10X
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