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Manual Do Microscópio

1. Olympus introduces its new System Microscope Series BH, developed with an emphasis on advanced design, excellent durability, and unsurpassed performance. 2. The Series BH offers three basic models with interchangeable components like nosepices and light sources to provide flexible configurations. 3. A wide range of accessories are available to expand the microscope's capabilities for various applications like brightfield, darkfield, and photomicrography.

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0% found this document useful (0 votes)
123 views

Manual Do Microscópio

1. Olympus introduces its new System Microscope Series BH, developed with an emphasis on advanced design, excellent durability, and unsurpassed performance. 2. The Series BH offers three basic models with interchangeable components like nosepices and light sources to provide flexible configurations. 3. A wide range of accessories are available to expand the microscope's capabilities for various applications like brightfield, darkfield, and photomicrography.

Uploaded by

Johnny Javier
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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In response to numerous demands &am the scientific and

industrial communities, OLYMPUS, a leading manufacturer


of quality microscopes and other precisian imtrumen'ts, takes
p a t prides in introducingthe new System Microscope Series
Brl.
Development of the Series Bh .m~croscope$has been a~
cornplished by the joint efforts of industrial designers and
the ercperiened OLYMPUS a p t i d and mechanical engineer- .
irig staff, with particular emphasis on advanced design, exeeI-
bnt durability and unsurpased performance, and profiting
from the innovative fechnolugy OLYMPUS has applied in the
manufacture of the Universal Researcfi Microscope Mae[-
VANOX.
The Series BH offers a choice of three basic vmiom, Modds .
BHA, BHB and BBH. he
* gf
BHA. features an inter- '>-7
chmwable nosepi=, an inwchangrble 30W low v d t a g $!
light m um and a highllow magnification selector, and the alJ
MQdBl B f S features an interchangeable 3OW low
votbge tight wurce and a highhow rnagni-
Ficam sahtw, while h e Model
w has a: Fixed 1w low voltage
l i muwe md rn highllow
f m @ F k a h A *tor.

.,

1
I
Olympus System M i m p e Series BH
transformer h i l t in the base, a unique c ~ d pr-s
q
applid to the prisms in tfie abservadan t u k ta ~~ light
ents such as microscope tranmi~iontnm than twice w much as in mnwntimal
observation tu& elk.
Every part of the Series BH mmpownts and a c m o r i s is
&signed with emphasis on durability; fix imtanwJs ~ o 8 t h
lunnink wear resistant ball guides are employed in the f w w
mudular, buil&rl~blwksystems provides ing and stage operatian mechanisms, and the light wm
m k r m p i c and pbtom icro- built in the km incorporates a thyristor circuit fw 5bMliza-
#a& ambhtatiom m a suitable for your requirements, to tion bf power wpply.
, <.-.yWr at a reatSstEc figure; and to further expand 3. Amesswies for Expanded Capabilities
e6,m.qsb@w of the micrmcuge by addig msonably I
Dqendng upon your requirements for not only general but
to specif~cpurposes. atso specific purposes, vwious accessories are available for
bri@tfield, darkfield, fluorescene, polarization, phase con-
. trast, d8fferential interference contrast fur transmitted and
~ ~ t light;e dsuper widefield, dual viewing; and p h o t ~
miwogaphy, automatic and manual.
4.. V-dIe Camera System
The System Camera Model PM- 'I0-A with automatic film
~'advana fadliiates your photomicrographic work, solving
, the dtficult color temperature adjustments often encounter-

ed 5n photomicrography. A unique cdw temperature meter-


; I* and regulating device (CTR) baIanm the wlor tempera-
- ture of the film with the color temperature of the light
ice for ASA incorporated in
d uni& permits fine adjustmerntpf &A film speeds
crepwnt range. This modular sy&m prmits rapid
x 4%'' and 4" x 5" she fnm.
-

Excellent Wfbrmance Tn
Ob~~atio uk
Rtvalving Nosepis&
sfiw

The Series BH surpasses optional objectives or vice versa, according


conventional microscopes in 4 to various microscopic applications, without
removal of the objectives from t h e nosepiece.
its exceptionally functional
design. Objectives for different methods of micr*
scopic observation can easily be mounted on
e extra nosepiece in accordance with
W OBSERVATION TUBE your specific requiremen%. These
A trinocular tube (inclina- objectives include plan apochroma-
tion 45") is standard for the tic, phase contrast, metallurgical,
Mode[ BHA, for observation and and differential interference con-
photomicrography. The A
maximum field number is
21. The tube is rotatable
through 360°, with
clamping screw, permit-
ting fatigue-free observa-
4 e The standard sQge i s a
large, graduated, square
methanical stage, 142mm x
tion of an exceptionally 1 340mm, on dovetail stage
large field of view. bracket, with clamping
A unique coating process of lever and large coaxial low
the prisms in the observation tube drive controls on the right
reduces light loss to a negligible amount,
thus increasing tight transmission by more - 1 he tmversing area is 52mmx
than twice as much as with conventional Amm, with low positioned, coaxial
observation,tu bes. C- control knobs. Traversing movements on ball
Interpupillary distance adjustment from 53mm to guides, with verniers reading to 0.1 mm.
72mm. A diopter adjustment ring i s provided on both
eyepiece tubes to maintain objective parfocality a t different
interpupillary distances.
A light path selector lever directs either 100% of the light
to the observation tube, or 80% to the phototube and 20% to
the observation tube.
I
A circular dovetail mount permits interchange of other

.
observation tube5 with ease. A binocular tube and a super
widefield observation tube are available optionally.

REVOLVI NG NOSEPI ECE


Quintuple revolving nosepiece on ball races with dust-proof
carrier. Smooth rotation maintains parfocality of all five
objectives. The click stop is positive in action, so that each
objective comes to a common center when rotated into
working position.

Large specimen holder, capable of simulta-


neous use o f two 26mm x 76mm specimen
slides, i s removable to obtain a large un-
obstructed stage surface.
.The dovetail stage bracket permits easy
A dovetail slide mount o f the microscope stand Model attachment or removal of the stage. The horizontal dwe-
BHA-F permits removal of the quintuple nosepiece which tail mouQt facilitates interchange of optional stages in
facilitates interchange of the standard objective set with - accordance with various observation methods.
Excellent Ikdbmance
ICUTAWAY VIEW OF THE OPTICAL SYSTEM
Focusing Adjustments

Base

W FOCUSiNG ADJUSTMENTS ~ ~ . ~ e h . n f i s u
The I* qPU#rtment
*The dual coarse and w@ehnhrn opewee a
~ P wm-
I O C W plnwn -
fine focusing knobs mow to thrs and goarser
a rutmefit motion, o h
are coaxial and aYour+tage -re#-
tdon a Y t t @ m TAb unC
designed to share fgn permla fins
a common guide-
fW 2
o c u I ow? #he e r r W
rnF offha r n 0
=us=# rnoktrrsr~ta n J i
way to give a g @ t e a l l ~+e h m
c-h.
smooth, even The e w n e ddwtment
~mschonitm wrorks dl-
motion through- m t l u on the foclrplng
wbiuh laQtas
art the whole range FCik r ttac+hg~
~krF mouutw block'
iq t b
of 40mm. b$ -I& the .red&
T h e coarse adjust- S,E*i;. zi:
ment knobs are
56mm in diameter for
easy operation.
The fine focusing adjustment i s
graduated in minimum increments o
Zp and incorporates special reduction
gears for smooth operation and life-
time reliability.
The automatic pre-fwusing lever is
provided to prevent possible contact
between specimen and objective as
well as to simplify coarse fmusing.
The lever is tocke
This prevents turther upward travel of the stage and auto-
matically provides a limiting stop if the stage is lowered and
then raised again, Ihe automatic prefocusing lever dms not
restrict fine focusing. lBASE
.A tens~onadjustment ring i s provided next to the right The dua-pfE10f 5quaM
hand coarse adjustment knob. With this device the tension of base incorporates a h ighllow magni-
the coarse adjustment is treely adjustable for either heavy or fic+tion selcctor system and a l o w vuluge iramformer. The
light movement depending on operator preference. "omoff" switch, voltmeter and sliding control lever for
continuously variable light inten~itiyare hit t in the bde Tur
effortless operation.
The highllow magnification selector lever enables Koehler
.CONDENSERS type illumination throughout the whale range of objective
A ring mount with clamping screw and centering device powcrs, from 4X t o 100X, without rnanipulatiur~uf the
accepts interchangeable condensers, standard or optional. auxiliary lens or interchange of condensers.
Rack and pinion height ddjustment, with condenser height .The in-base illuminator serves to direct light of the
displacement up to 32mm. appropriate quality and intensity, -emitted from a precenter-
*The standard condensers include the achromaticlaplanatic ed tungsten bulb, towards the microscopic object to ensure
% condenser N.A. 1.40, the Abbe condenser N.A. 1.25, and the even illumination o f the field, This in turn results in
super widefield condenser N.A. 0.95, depending on the photomicmgraphic negatives of optimum contrast, briltiance
I* . model of your choice, for optimum illumination of the
... and uniformity.
.- -
specimen from low power 4X to oil immersion lOOX The built-in transformer, variable from 0 V to 10 V,
objectives. Furthermore, the achromaticlaplanatic condenser incorporates a thyristor circuit in its rheostat far smooth fine
&, is corrected for chromatic' aberration, spherical aberration adjustment of light intensity.
and curvature of field, and is provided with a graduated .The standard light source incoporates a 30 watt pre
aperture iris diaphragm, decenterable for oblique illurnin* centered tungsten filament bulb, provided with a socket for
tion. It is recommended for work with high quality apo- positive contact, el irninating the problems of defective
chromats and plan apwhrornats. contact and overheating. Interchangeable with optional light
Ei?her one of the condensers, ahave, is easily replaceable sources such as high intensity halogen bulbs, mercury burners
with other condensen such as darkfield, phase contrast, etc. and eltctronic flash bulbs. a. :F 1
r. d -
Wrn Chart of Interchangeable Components 1
BH-0145 BKB130 Bli-TR45 BH-TR30 BH-SWTR BWPT
Binocular t u b (45') Binocular tube (3@) Trinocular tube (45') Trinocular t u b (30') Super widefield Vertical photot~be
winocular tube (30")

1
BH-Do M A AKFA BWFA AWN..
Dual viewing
'

Magnification Barrier filter Barrier filter Differential !


bod!' changer attachment attachment Interference contnst (
(simple type) attachment for
transmitted light '
,

BH-SRP BH-SRG BH-SV , ;BKSVL BH-SH


Circular rotatable stage Circular rotatable Square rnshanical Square mechanical Square rotatable
stage graduated s t a s with right-hand stage with left-hand mechanical stane
low drive c0ntrol5 low drive controls with horizontal
drive controls

..........................................................
IIH.CD BH-AAC BKSWC BH-DCD BH-DCW
LOW power Abbe condenser Achromatic/aplanatic Super widefield Dry darkfield
condenser condenser condenser condenser conlmr

BKPC BH-NC
Abbe phase contrast Differential Polarizing condenser
condenser interference contrast
condenser
.. .I...i........... .....................
- r. I

-
kP7R
I r~noculart u h for
I
I
= PM-CP
3-114"~4ll4"
Polaroid@film holder
1
be' polarizing light (30")
PM-C4~5
BKPBI L lnttrrnediate adapter
for 4 * xS* holders
I
L b
PM-1OA M I
Photomicr?flaphic Photomicrographic
'., BKMPS system camera system camera
i r :Projection screen (automatic) (manual)

I
I
I
....................................... b

---,-
I) I I I
[ a -
T5 - j
4 I: AI+-PA BKWL BH-RFA
I
Intermediate Polarizing filter Vert~calilluminator
Drawing attachment
A
i polarizing tube set for fluorexenre BH-mRF
F Iuorescenr~
BI-l-PRE
Revolving
................................. lamp house
nowpime BH-RE
for polarizing ReVblVing
Iight nosepiece

...........................
-................................k
- 1

Microscope
stands
BHA-F BHBF BHGF
B A
I

dE1
I

I530
LS15
Tungsten bulb Tungsten bulb
BH-LH BHLSH 6V .- 8V, 30W 6V, I S W
30-watt tungsten lobwatt halogen
lamp house lamp hwse

- - . - 1-
MICROSCOPE STANDS BHA-I
Extremely sturdy construction, functional design, ripple tone
finish, acid resistant. The differences between these three
stands are the interchangeability of nosepiece andfor light
source with the Models BHA and BHB, respectively.

Revolving nosepiece

Coarse and
fine adjuszment and fine adjustments 40rnm, on ball guides.

45mm diameter filttrs and a polarizer can be mounted on the light exlt on the baa.
Filter mount An analyzer can be inserted below the observation tube.
I
In-base Illuminator without
In-base IIIurninator, with high/low rnagniflcatlon selector, magnlflcation wlecror. L

lllumtnatlngsystem - -

- Obieotive powers variable from 1.3X to 10OX with interchmgo of condensom.


Maximum field number 29.
30 watt pre-ccnterd tungsten fllamsnt bulb, Interchangeable with 15 watt pre-centered tungsten bulb,
Light source varlous light sources. cordlag, not Interchangeable.
. Incorporates a thyristor circuit for fine voltage adjuntmant
Transformer
built Into base I. Voltage variable from OV to 8V.

Dimensions 300mm (helght) x 21Smm (widthj x 280mm (length)


Wtiahts 5.6 h 5.9 k. 5.7 kg
OBSERVATION TUBES
The observation tubes are designed to
match the streamlined appearance of
the microscope stands for ease of
operation, reliable performance and
outstanding durability. The binocular
and uinocular tubes are available in
300 or 45' inclination.
A 30'-inclined super widefield tube is
also available to scan a flat field of
view 260% larger than with the stand-
ard WF widefield eyepieces. This is
very convenient in biological research
as well as routine work, for it will
increase the efficiency and speed of
microscopic work without undesirable
eyestrain.

Maximum
field number** 29 21

Dlopter Adjustable
diopter setting
Both observation eyepiece tubes equipped with variable diopter adjustment, -
adjustment compensating for tube length variations.
on SW cyepieccs.
Convergent angle O" (parallel optical axes in binocular tubes) -
InterpupilJary 56 - 74mm,
distance 53 - 72mm, graduated.
adjustment graduated.

Two position selector lever; to direct 100%o f the fight


Light path
selection to observation tube or 20% to observation tube and - - -
80%to the phototube.
Attachment I Circular dovetail.
Rotation Rotatable through 360°, on circular dovetail.

B H E t are interchangeable.
- -

&phMlc*l lens
CoRecttsr
F W %erJaStobEt PwforW
Vartous filters availabld I Frosted f l i e r buift-in

Microscope stands BHA-F, BHB-F 1 BHC-F


MAGNlf lCATlON CHANGER MODEL AH-CA
The Model AH-CA incorporates 1X, 1.25X, 1.5X and PH
(phase contrast) positions to permit magnification changes
without change of objectives or eyepieces, as well as easy,
accurate alignment of tight annulus and phase annulus
Lenses 1X, 1.25X and 1.5X are provided on a rotating turret.
Centering telescope built in "PH" position, focusabla
7
BARRIER F I L E R ATTACHWENT MODEL AH-FA
Barrier filter attachment, mounted &tween nosepikc and
observation tube, with revolving turret containing five
I;
apertures, permanent1y mounted barrier filters L-420 and
AFC on slide, and Y-495, 0 - 5 1 5, 0-530 and R-610 built in
turret.

r BARRIER FILTER ATTACHMENT MODEL BH-FA


This attachment incorporaks the barrier filter L-420
permanently, to be mounted between microscope stand and
observation tube for fluorescent microscopy with various
exciter filters and barrier filters, optionally available. Two
slots fw inwtion of barrier filters, and UV protective shade
provided.

DIFFERENTlAL INTERFERENCE CONTRAST 'I


AlTACHMENT MODEL BH-IYIC
The interference contrast intermediate tube incorporates the
m d i f i d Wolldston prisnl and iinalyrer with depolarizer for
1
Homarski differential interference contrast microscopy. ,

DUAL OBSERVATION ATTACHMENT MODEL BH-DC


The Model BH-DO was k i g n c d with the teacher, the
research team and the discussion group in mind, for simulta-
neous obxrvation with your partner in your research work,
education and on-thespot training, etc., as wdl as photo-
micrography.

r DRAWING A+ACHMENT MODEL BH-DA


This attachment .can be easily attached between microscope
stand and observation tube, M that you can simultaneously
observe the images of your drawing pen and the contour of
h e specimen to be traced.
Drawing dzc
Eyepiece Total magnification (diameter
FK2.5X Objective power x s 72 T
Objcctbc power x 6.6
F K5X Objective power x 10 144

FLUORESCENCE VERTICAL ILLUMINATOR


ATTACHMENT (afDar Ploem) MODEL BH-RFL
The Model BH-RFL comprises a vertical illuminator w i h a
1M3W high pressure mercury burner, to further extend the
versatility of the Series BH for incident light fluorescence
microscopy.
Super Widefield Attachment /MODEL BH-SW
The SW attachment consists of the A super widefield condenser N.A.
super widefield observation tube (in- 0.95, for use with SW objectives,
clined 3 0') with vertical phototube, eliminates necessity of immersion for
use even with lOOX objectives. If it is
paired BiSWl OX eyepieces, photo
eyepiece FK3.3X, Plan Achromats 4X, - desired to fully utilize the N.A. of the
10X, ZQX, 40X and SW Plan 100X(oil) 100X objective, it is recommended to
and SWC condenser, for a field of view use the achromaticlaplanatic con-
more than twice as large as the area denser B H-AAC.
previously considered to IE the ulti- Each eyepiece is provided with diopter
mate. The SW1 OX eyepiece scans a flat adjustment. lnterpupillary distance i s
fieid of view 217% larger than the variable from 56mm to 74mm.
standard WFlOX widefield, while an The super widefield objective SW Plan
optional SW7X scans a 26055 larger IOOX enables not only the obser-
vation of the widest field o f .view in
field than the WFIOX. No more time
consuming scanning of smatl specimen
areas with the SW attachment! And
4
u,d,A.+
D conjunction with the SW eyepieces,
but also produces a f l a t image to the
what is more important, working with edge of the field with excellent resolu-
the Made1 BH-SW will increase the tion.

-
efficiency and speed of microscopic
work but will not introduce undesir-
able eyestrain.
STANDARD EQUIPMENT
Super widefield trinmular tube, inclined 30a,
A with vertical phototube BH-SWTR ............................ 'I
Super widefield condenser, N.A.0.95, with
I graduated aperture iris diaphragm BH-SWC ................. 1
Super widefield eyepieces BiSW1OX, paired ..................... 1
Photo eyepiece FK3.3X .......................................... 1
'Objectives: Plan 4X, Plan IOX, Plan XIX (spring),
Plan 40X (spring), SW Plan 100X (oil,
spring) (set of tive) ................................... I
Optional Accessory
Super widefield eyepieces SW7X, paired
*If your BH Microscope is equipped with Plan objectives,
objectives mentioned abwe except SW Plan 100X may be
omitted.

'i
Fluorescence Vertical Illurninator Attachment (after Ploem)/MODEl BH-RFL
The Model BH-RFL incorporates a
hi$ pressure mercury burner, a wide
range of exciter filters and barrier
filters in addition to all or either of 4 '
dichroic; mirrors for ultraviolet, v i e
let, blue and green excitation. The
dichroic mirrors, originated by Dr. j.
S. Plaem, are hilt in mounts for
selection of the desired wavelengths for
excitation of the specimen. Radiation
energy emitted from a powerful 100W
high-pressure mercury burner is trans-
mitted through wllectw and exciter Cat Brain, Acridlne Orange
filter to the dichroic mirror to excite
the fluorochrome in the specimen in
the desired wavelength. After irradia-
tion with the excitation wavelengths,
the specimen emits a longer visible
wavelength, due to Stoke's law. A
barrier filter between objective and eyepiece passes tbe

-7

I
- ., ..., Interference Contrast Attachment for ansmitted Light/MODEL BHNIc
The Model BH-NIC is designed for the tours more difficult The Nomarski
qualitative study of transmitted li&t method does not cause halos, so that
phase objjcts with the System Mime a clear definition of image details is
=opes Series BH. This differential obtained.
interference contrast device incorpo- .The Nomarski method permits utili-
rater a pair of birefringnt crystal zation of the full numerical aperture
prisms as beam-splitters, with a shear of the objective, which results in ex-
between the t w o interfering beams ceptioml image brightness and re-
smaller than the resalution limit of the solution almost double that of phase
objective ussd in the microscope, w contrast.
that no double image is observed. q- .The depth of fwus is smaller in dif-
The interference contrast method ferential interference contrast than
r ~ n d e ra~ sharply defined, relief-like in phase contrast. This prevents dis-
image with excellent contrast in a wide turbing out-of-focus effects, and
ran@ d interference colors. This permits layer by layer scanning of
method pwmits obmvation of un- specimens, commonly called "&ti-
stained transparent objects like phase cal sectioning".
contrast, which makes i t useful for o b Interference colors, or grey shadings,
sewation in histology, cytology, biw can be seen in proportion with the
logy, anatomy, etc. Furthermore, when used with the Series gradient of path differences of the specimen. P h m con-
BH, this attachment permits detection of minute differences trast helps to determine a high or low refractive index of a
of thickness and changes of internal refractive indexe-s of specimen detail by the appearance of halos, wording to
unstained, tramparent specimens By operating the bi- the positive or negative contrast of the objective in use.
refrinant crystal p r i m built in the intermediate t u b , a most
suitable contrast color can be chosen ,in accordance with AE t h e shearing direction i s restricted in accordance with
purpose and specimen. The background w l o r can be w n - the sliding directinn nf the Wnllaston prism, it is preferable
tinuwsly changd in the order from dark to yellow, to to use a rotatable stage to wient the specimen in the
purplish red, to blue or in the reversed order. direction b e s t suited fob optimum msolution.
Features For the observation of anisotropic objects, it is re-
+Generally the larger the phase difference, the more con- commended tn apply phase contrast rather than inter-
spicuous the halos, which is a drawback of the ordinary ference contrast for better results.
phase contrast method, making distinct observation of m-
STANDARD EQUIPMENT
Differential interference contrast intermediate tube
AH-NA. .........
%- Differential interference contrast condenser BH-NC
1
.........1
I Phase contrast objectives: PC PL Plan 10X .....................
PC PL Plan 40X ..................... 1
1

PC P t Plan 100X (oil) ............. 1

I Centering telescope CT ................................................ 1


Filter 45G533 ............................................................ 1

phase Contrast Attachment/MODEL BH-PC


-
fiD.A. Rb'EQ"iP NT
Indispensable to observe the internal structures
Pha# contrast turret condenser,
of cells, nuclei, or minute living objects with N.A.1.25 ..................................
Centering telescope
1
...........................
1
various contrasts as desired, the Model BH-PC
Phase conwast objectives:
permits phase contrast microscopy by means of 7 *Positive low contrast
phase contrast objectives and a phase contrast PC PL ~ c 1hOX, PC PL ~ c ZOX,
h
PC PL F L 5-40X (spring] PC PL FL
condenser. Additionally, the attachment permits 5-100X (spring, oil] ...........,.....
*Posltlve low low contrast
t
normal brighttield and darkfield microscopy.
PC PLL Ach IOX, PC PLL Ach ZOX,
The Model BH-PC is equipped with an Abbe
type p k turret condenser, N.A.1.25, with
a PC PLL FL W O X (spring), PC PL FL
5 1 00X (spriri~,oil) ..................
1tam

saduated aperture iris diaphragm, a ring centering 7- *Nagatire high contrast


PC N Y Ach IOX, PC NH Ach 20X,.
&vice, and annular diaphragms for 1OX, 2W, 40X 1 PC NH ~ t S-h OX (spring), PC NH ~ c h
5-10DX (spring, oil) ...................
,...1 sa.

I
and 100X phase objectives, which are available in *Negative medium contrast
4 contrasts, PLL, YL, NM, NH, and an empty PC NM Ad1 1 OX, PC NM A G 2~0 X ,
PC NM Ach S-40X (spring), PC NM Ach
aperture for brightfield observation. 5-1 00X (spring, oil) ...............,.,.
*The Model A-PC-PA consists of all the 4
1 ea.
'Ihese letters designate the following contrasts as listed above, while one con-
contrasts: N = Negative; P = Positive; M = trast combination can also be available as
Medium; L = Low; LL = Low-Low; H = High. fulluws:

1
B-PC-P&PL positive low contrast)
This exceptionally wide selection o f combinations is B-PC-PB-PLt positive low low contrast)
I certaln to s u ~ the
t most demanding requiternenrs. &PC-PB-NH r~eg.ilive I~lgllL L H I ~ ~ ~ L )
B-PC-PB-NM negative medlum contrast)

ttter Contrast Specimen Purpow

PLL Positive- If thc aperture diaphragm is stopped Obwnation of


Low-low down slightly more than tho objective forms and inter-
N.A., image contrast may be Increaxd. nal structures of
I f further contrast Is needed, this PLL specimens wlth
contrast is recommended. relatively high
contrast.
PL en specimens require definite obsewa-
low tion of their internal structure more than
a t slightly stopped down aperture dia-
phragm of brightfield,

NM Negative- When more contrast is necessary for ob- Observation of


medlum servation o f objects so small that the forms, details,
extremely stoppeddown aperture dia- and motions of
phragm can barely detect their existence. low contrast ob-
jects.
NH Negative- When darkfield observation is preferable
hidl ta brightfield, since the obbc& are very
low in contrast and cat hardly be ob-
f 4 d in brightfield.
Dual Otrservation Attachment/MODEL BH-DO
Specially designed for the demands of teaching and disus-
sion, the Model BH-DO has two binocular tubes. Both tubes
have adjustable interpupillary distances and all four eyepiece
tubes have diopter adjustments for individual comfort.
The entire dual observation assembly rotates -through 360".
Discussion is facilitated by a built-in iIluminated pointer,
which orients or rotates at the tough of a manipulator, with
yellow and green filters to intensify pointer contrast. High
eyepoint widefield eyepieces WFlOX are supplied for maxi-
mum observer comfort, without standing color correction
and field flatness.

II
hkroscope stand ,

1, Transformer

Dual viewhg body w i t k i k g a l BH-Do 1 1 1 1


pointer and poi* manipulator
Binocular tube, inclined 30" BH-BI30 1 ---
Binocular tube, Indined 45" BH-B145 - 1 - 1
Trinocular tube, Inclined 30' -
BH-TR30 - 1 1
Eyepieces, H.EP. pairkd BiWFlOX 1 1 1 2
Photo eyeptwm FK33X -- 1 1
Transformer, voltage variablt at 2V-4V-6V TDO 1 1 1 1
Tungsten bulbs 6V I o w a 3 3 3 3
For those wko wish to use the standard observatbn tubs of *he
microscope Series 6H, ~ p 1& , e2 or 3 is r w e n d e d .
-
m . --
fl --%
Polarizing Attochmmt/MODE L BH-P-11 BH-P-2
The Polarizing Attachments Models BH-P-1 These attachments feature a removable quadruple revolv-
I
and BH-P-2 are designed to enable the user ing nosepiece which is provided with centering devices
of the System Microscope Model BHA to for all objectives except the 4 X objective.
microscopicatly observe the optical charac- The polarizing intermediate tube (magnification
teristics of a suitably prepared object in power l.OX), compatible with various test
polarized light. As tabulated below, the plates, incorporates a fmusable Bertrand lens

-
Model BH-P-1 includes a binocular tube for mounted in a rotating turret, with a depolarizer
polarizing observation, while the Model which makes the binocular vision of the spe-
8H-P-2 indudes a trinocular tube for both cimen evenly black between crossed analyzer
polarizing observation and photomicr* and polarizer.
graphy, in addition to the standard equip-
ment commonly shared between them.
Each of these attachments facilitates mi-
b The multi-layer coating applied to the
optical elements in the observation
tube's BH-PB1 and BH-PTR assures ex-
croscopic work in polarized light not ceptionally bright binwular vision.
m l y in the fields of mineralogy and
petrography, but also in chemistry,
pharmacology, biology, medical science
and ceramics, as well as in the chemical
and textile industnies.
657 To those who do not yet own the BHA

BHA-751-P are
micrompe, Models *HA-651-P or
therecommended.

b *
6'
I Projection ScreeniMC!@@
I.

A trinocular microscope can be fitted with this projectim


screen. It projects the magnified image of a specimen onto a
155 rnm d i m . s c r e e g a p viewing, consultation, cfC.
The image magnification on ween equals
objective power x photo eyepiece power x 3.
STANDARD EQUIPMENT
Projection screen AH-MPS ......................... -1
Photo eyepiece adapter PM-ADF .................... .I

1
Photomicrographic System Camera 1 MODEL PM-10
The PM-10 System Camera combines the features and
advantages of many separate conventional attachment
cameras, ranging from 35 mm camera back to 4" x 5" film
holders. The Model PM-10 consists of two basic units-a fully
automatic version Model PM-1 @A and a popular manual
version Model PM-10.M.
The modular design concept of the system camera permits
easy interchangeability of many accessories. When these
modular accessories are attached to the automatic or manual
photomicrographic bodies in accordance with your require-
ments, sharply focused pictures on standard 35 mrn film,
3%" x 4%" Polaroid film or 4" x 5" size film can easily be
obtained. The data imprinting 35 mrn camera back can, be
easily attached on the exposure body. Once in place, the
back enables the user to imprint informarion such as
numhrs, sy mbots, etc., on the film.
The Model PM-IOM and the Attachment Camera Model
PM-6 incorporate a light measuring port to accept the
exposure and color temperature probes of the Exposure
Meter Model EMM-7
0 & * 5* lnPs;msdla& ~wqpw
l( 1 4*~rr 5' ~ckoid@filmb k , G M m i c fnm holder,
der, G r a p h k h t film holdor) ( P W )
Back [PMEP)
@ 35mm Crmsn Back vlilk Manull film M c o (G35J
@ 35$lm k c k ~ 4 t hAutomatlf F i h A&- (C-3M)
0 ~withRL*y~fworFwmt~oElu(PM-DL)
@ Ahptw for 3 5 m 1- Bpck wIUI Manual Fllm Muarm (PM-D35)
@ fff 35mm b m f a Back vrih Automtk FIlm Ad*- (PM-DSSAJ
@ Cbntrd Unit for Fully A u m a t l c 35mm G m r a Back {PMEBA)
@ Expmum M b t a (EMM-7)

- Automatic txposum body PMPBA.


Adapter for 3Smm camera back wlth
-- Manual exposure body PMQBIW.
Adapter for 35mm camara back
3Smm attachment camera, ~omprlr-
ing manual expowre body.
automatic fflm advance PM-D35A. with manual film advance PM-D35.
35mm oamcra back with automatic 35mm camera back with manual
Components . film advance C-35A. '

Control unit for fully automatic


35mm camera back PY CBA.
- film advance C-35
Others: Focuslng tetescopa, F o c u ~
Ing magnifter, Eycplacc adapters,
Others: FocuJng telescope, Focus- Filters.
ing magnifier, Eyepiece adapters,
Filtars.

35mm cammra 1 Autamatk Rrn advance with built-in


motor.
I
I
Manual film advance.
back
Information Imprinting device, self-setting frame counter, rewinding knob, film size 24mm x 36mm.
-
Light path 3-pwltlon Ibght path selector.knobf6r obslrvaton, obseivation-and-photography, afi'd exposure measurement.
salection
- - - -

T h e control unit is a console type


with switches for automatic ex-
.The manual exposure body is provided with a IIght measuring port to
accept the exposure meter Model EMM-7 which facilitates the detsrrnina-
pwure, time exposure and flash tion of corrcct exposure time and color tempemura.
synchronization; ASA film speed
Exposure
' +selectordlal for different films - Tho exposure msmr Model EMM-7 with direct reading scales for 35mm
exposure measurerntne (High: 11250-112 m. Low: 1/2-32 wc,);
& color from ASA 6 to ASA 3200; ASA
tempraturc fine regulation dial, and zero- for large formats (High: 1130-4 sec. tow: 4-1 28 s.)
controls r a t t i n g cdor temperature meter- Calar temperature meter for light balancing.
Fins adjustment of ASA film

t
W.. T
speed 0.75,0.85, 1,1.2, 1.5.
Automatic comptnsatfon for
reciprocity failure.
Automatic exposure range 32 min. B and 1 rec. to 11250 sec. with integrated mechanical shutter in vibration
1 to 11100 stc. Push button for proof rubber mount
tima automatic Buttar retern, to elirni-
k natc shutter vibration.

L a m format Poimid 3%"x 4%"' 4" x 5" sheet film holdon, film pack holder. Not available.
attachments
Optical Characteristics
I n keeping with the varied requirements of the users, EYEPIECES
Olyrnpus makes available objectives of high resolution from TYae 1 Initial Magnification I ~ i e l dNumb
1.3X to 1OOX magnifications. They include Achromats, Plan
Achromats, Fluorites, Apochromats, Plan Apochromats and Compensating K20X 7-5
Super Widefield PIan Achrornats. Especially, the PIan Apo- BiK2OX 7-5
chromats are capable of producing a flat image to the edge of WF 10X 18
the field, with excellent resolution, free of field curvature. WF 1OXMicro 18
Chromatic aberration is corrected for three colors, spherical
aberration for two colors. The best objectives suitable for the
Widefield
I (with micrometer 10]100rnrn)
WF 15X
BiWF 1 OX 18
m&t sophisticated demands. BiWF 15X 12
All eyepieces are computed to match the objectives for full
chromatic and distortion correction. They are Compensating, High Eyepoint H.E.P. WF 1OX 18
Flat Field High-Eyepoint, Super Widefield, Photo Eyepieces,
Widefield H.E.P. BiWF 10X . 18
etc. BiSW 7X 29
Super Widefield BiSW OX 26.5
Photo FK 25X, FK 3.3X
FK 5X. FK 6.7X

Initial Magnification
-
4) 0.10 I 19.87 mm
1ox
20X
40X
60X
1OOX (oil)
S4OX (spring)
S-60X (spring)
5-100X (spring, oil)
1-100X (iris diaphragm)
No cwer Ach 40X (for use without cover glass)
J
*- FL S-40X (spring)
'-' 3 FL S-60X (spring, dry, correcting collar)
F I u a e s (Semi apocbroma**) FL S-1 ODX (wring, oil]
NOcover FL 40X (for usa without covet glass)
1ox
Achromats
mx
S-40X Irprina)
Phase Contrast S-100X (spring, oil1
Fluorites FL 5-40X (spring)
(Semi apochromao) FL S-100X (spring, oil)
Apo #X (spring, dty, catrestlngcdlar)
Apo 40X (spring, ail, iris diaphragm)
Plan 1.3X
Plan 2X
Plan 4X
Plan 1OX
Plan A c R m a t s Plan 20X (spring)
Super Widefield Plan 4UX (spring)
sw W ~ I II oox isprina oil) 1 ,2S 0,08
Plan 100X (wring, oil ) 1.25 0.14
Plan ADO4X 0.16 4.35
Plan A ~ 1 OOX (s~rinel 0.32 0.16
Plan Apochrmts Plan Apo 2QX (spone) 0.65 0.14 .
1
Pian Apo 4&X [spring, correctingcollar) 0.95 0.10
PIan Apo 10 0 X (spring, oil) 1.30 0.09
*: "'
Combina-tionsof Various Components 4
I,.

BHA-F

mrcrmcope stand BHB-F

BHGF
I

-Quintuple revolving nosepiece BH-RE


t
30 watt tunsten lamp house BH-LH

b u n g s t e n bulb 6V, 30W LS30

--
A.

Binocular tube @")#. *' BH-B145


. --
Trinocular tube (45') BH-TR45

Mechanical stage BH-SV

Abbe condenser BH-CD

AchlApla. condenser BH-AAC


_._ I.
Achromat 4X

Achromat 1OX

AcRrmat S-40X (spring)


R -
Achromat 5-1WX (spring, bil)
L

- Objectives Ran Achromat 4X

Ran~chrbmat 10X

I Plm Achrornat 20X (rprina)

I Plan Achrornat 40X (spring) I


I Plan Achromt 1M))( (spring, oil)

Eyepieces, H.E.P. BiWFl OX, paired


I
Phota eyepiece FKSX
Filter (45C)

Spare bulbs and fuses, 2 pieces each


Immersion oil and vinyl dust cover '
OLWMPUS OPTICAL Cd, um.
43-2. Ha-ya Zchome. Shibuybku, Tokyo. Japan
o~vmws
OPTK=ALco.(EUROPA) OM&.
2 Hamburg 1. Steindamm 105. West Germany
OLVM- CORPORATION OF AMERDCA
2 Nevada Drive, N m Hyde Park, N. Y. 11040, U.S.A.

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for m y pmpb the world of O l y m B is the wartd
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p m f o m e with f h b mrt- ..and hemiWM.
., l%i*~m'&rnst, -.k

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i R iki r Cgmimt to MI^
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frPm th&njpWOM-wstern SLRcamera
%athe r n ~ I t ? - ~ rB dn~ l ~ ~ ~ ~ i r n ~ .
At @vrnw, "Fra~m tFImgh Prekjsirn'' 1s
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