Scribd
Upload
164 views

Group No.: Total Score Group Members:: E. Coli Smear Preparation. On Microscopic Examination, How Would You Expect This

Basic dyes are more successful than acidic dyes in staining bacteria. Simple staining can identify morphological characteristics but not other properties of microorganisms. Without heat fixing, an E. coli smear may not adhere well to the slide. The steps in Gram staining are standardized to reliably distinguish between Gram-positive and Gram-negative bacteria. Heat or a surfactant is needed for the primary stain but not the counterstain in acid-fast staining. Knowing cellular structures like capsules, flagella, and endospores provides important information about bacteria. Nigrosin does not penetrate bacterial cells.

Uploaded by

Roan Eam Tan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
164 views

Group No.: Total Score Group Members:: E. Coli Smear Preparation. On Microscopic Examination, How Would You Expect This

Basic dyes are more successful than acidic dyes in staining bacteria. Simple staining can identify morphological characteristics but not other properties of microorganisms. Without heat fixing, an E. coli smear may not adhere well to the slide. The steps in Gram staining are standardized to reliably distinguish between Gram-positive and Gram-negative bacteria. Heat or a surfactant is needed for the primary stain but not the counterstain in acid-fast staining. Knowing cellular structures like capsules, flagella, and endospores provides important information about bacteria. Nigrosin does not penetrate bacterial cells.

Uploaded by

Roan Eam Tan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 2

Activity 3: Staining Microorganism for Microscopic Examination

GROUP NO.: TOTAL SCORE


GROUP MEMBERS:

YEAR & SECTION:

Answer the following questions. See rubrics below to be used in grading your output.

1. Why are basic dyes more successful in staining bacteria than acidic dyes?
2. Can simple staining techniques be used to identify more than the morphological
characteristics of microorganisms? Explain.
3. During the performance of the simple staining procedure, you failed to heat fix your
E. coli smear preparation. On microscopic examination, how would you expect this
slide to differ from the correctly prepared slides?
4. Why are the steps in Gram Staining so carefully standardized?
5. What color would the bacterial cells have when viewed microscopically after the
following treatments:

Gram Reaction Crystal Violet Gram Iodine Ethyl Alcohol Safranin


Gram-positive
Gram-negative

6. Why must you use heat or a surface-active agent when applying the primary stain
during acid-fast staining?
7. Why is the application of heat or a surface-active agent not required during the
application of the counterstain in acid-fast staining?
8. Why is it important to know whether or not bacterial cells possess capsules, flagella,
or endospores?
9. Why doesn’t nigrosin penetrate bacterial cells?

SCORE CRITERIA FOR QUESTIONS


5.0 Question is explicitly answered with the correct rationalization.
3.0 Question is answered correctly with some misconceptions.
1.0 Question is answered but incomplete.
0 Question is not answered.
MAXIMU
QUESTIONS SCORE
M POINTS
1. Why are basic dyes more successful in staining bacteria
5
than acidic dyes?
2. Can simple staining techniques be used to identify more
than the morphological characteristics of 5
microorganisms? Explain.
3. During the performance of the simple staining procedure,
you failed to heat fix your E. coli smear preparation. On
5
microscopic examination, how would you expect this
slide to differ from the correctly prepared slides?

1
Activity 3: Staining Microorganism for Microscopic Examination

4. Why are the steps in Gram Staining so carefully


5
standardized?
5. What color would the bacterial cells have when viewed
5
microscopically after the following treatments:
6. Why must you use heat or a surface-active agent when
5
applying the primary stain during acid-fast staining?
7. Why is the application of heat or a surface-active agent
not required during the application of the counterstain in 5
acid-fast staining?
8. Why is it important to know whether or not bacterial cells
5
possess capsules, flagella, or endospores?
9. Why doesn’t nigrosin penetrate bacterial cells? 5
TOTAL SCORE 45

You might also like