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Lab Request Form

The document is a laboratory request form and standard operating procedures for various tests conducted at St. Leo's Hospital. It includes the patient's information and physician details for the laboratory request form. The SOP describes procedures for conducting tests in chemistry, hematology, microbiology and serology. It outlines steps for electrolyte analysis, complete blood count, blood grouping, malaria parasite examination and more.

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Eze Stephanie
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© © All Rights Reserved
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Download as DOCX, PDF, TXT or read online on Scribd
228 views

Lab Request Form

The document is a laboratory request form and standard operating procedures for various tests conducted at St. Leo's Hospital. It includes the patient's information and physician details for the laboratory request form. The SOP describes procedures for conducting tests in chemistry, hematology, microbiology and serology. It outlines steps for electrolyte analysis, complete blood count, blood grouping, malaria parasite examination and more.

Uploaded by

Eze Stephanie
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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ST LEO’S HOSPITAL

No. 57 Nike Lake Road (Opposite Amazing Love Church)


Trans-Ekulu, Enugu Tel: 08033401643, 08054227816)

Laboratory Request Form. Insurance or Private patient: To: Dept. of Microbiology

SURNAME(BLOCK LETTERS) FIRST NAME(s) FOLDER NO. DATE OF REQUEST

Ward/Clinic Physician/Surgeon Age Sex Tribe Nationality Religion

Provisional Diagnosis-------------------------------------------------------------------------------------------------------------------------------------
Clinical Details---------------------------------------------------------------------------------------------------------------------------------------------
--------------------------------------------------------------------------------------------------------------------------------------------------------------
--------------------------------------------------------------------------------------------------------------------------------------------------------------
Nature of Specimen--------------------------------------------------------------------------------------------------------------------------------------
Examination Required------------------------------------------------------------------------------------------------------------------------------------

---------------
Report Signature

ST LEO’S HOSPITAL
No. 57 Nike Lake Road (Opposite Amazing Love Church)
Trans-Ekulu, Enugu Tel: 08033401643, 08054227816)

Laboratory Request Form. Insurance or Private patient: To: Dept. of Microbiology

SURNAME(BLOCK LETTERS) FIRST NAME(s) FOLDER NO. DATE OF REQUEST

Ward/Clinic Physician/Surgeon Age Sex Tribe Nationality Religion

Provisional Diagnosis-------------------------------------------------------------------------------------------------------------------------------------
Clinical Details---------------------------------------------------------------------------------------------------------------------------------------------
--------------------------------------------------------------------------------------------------------------------------------------------------------------
--------------------------------------------------------------------------------------------------------------------------------------------------------------
Nature of Specimen--------------------------------------------------------------------------------------------------------------------------------------
Examination Required------------------------------------------------------------------------------------------------------------------------------------

---------------
Report Signature
SOP
A) CHEMISTRY
1) Collect whole blood, get the serum
2) Perform each test according to procedure
3) On the chemistry analyzer
4) Select the test
5) Put the probe into the prepared solution
6) Print.

Ai)
1) Prime the Electrolyte machine
2) Change MBM
3) C02 calibration.
4) Insert probe into the serum (avoid air bubbles)
5) Print.
HAEMATOLOGY

B) CBC
1) Collect blood sample mix in a (2ml) in an Edta bottle.
2) Mix in a roller mixer for 60secs
3) Input patient’s I.D in the hematology analyzer.
4) Take the sample to the probe for suctioning.
5) Print out result.

Bi) ESR

1) Mix 1.6mls of whole blood with 0.4mls of sodium citrate


2) Stand the western green.
3) Read after 60mins.

Bii) Blood group


1) Collect whole blood
2) Put equal proportion of whole blood to ABO antigen on a tile.
3) Rock and look out for agglutination.
4) Read.
Biii) Mp
1) Make a thick film on a slide allow to air dry.
2) Flood the slide with field stain A (for 1sec).
3) Couther stain with field stain B(1sec)
4) Air dry and read with x 100 objective.

MICROBIOLOGY
C) HVS
1) Use speculum to open up the vagina.
2) Insert 2/3 of your swab stick.
3) Make a stab on your culture media and strike
4) Incubate for 24 hrs.
5) Do sensitivity if any growth (24hrs)
6) Read the cleared zone
7) Use normal saline and make a solution; put on a slide and read with x 40 objective.

Ci) U/A
1) Collect mid stream urine in a universal container
2) Do the macroscopic examination (combi 9)
3) Put urine in a tube and spin
4) Decant and put the sediment on a slide
5) Cover with cover slip and read with x 40 objective.

SEROLOGY
D) (VDRL,HCV,PT,HIV,H.Pylori)
1) Collect whole blood into Edta/plain tube.
2) Separate them and get plasma/serum.
3) Dip the strip and read.

Di) Widal
1) Collect whole blood into a plain tube
2) separate serum
3) Add equal drop of serum to salmonella antigens
4) Rock for 3-5mins
5) Check for agglutinations.

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